标题
Cell-free gene expression: an expanded repertoire of applications
作者
关键词
-
出版物
NATURE REVIEWS GENETICS
Volume -, Issue -, Pages -
出版商
Springer Science and Business Media LLC
发表日期
2019-11-29
DOI
10.1038/s41576-019-0186-3
参考文献
相关参考文献
注意:仅列出部分参考文献,下载原文获取全部文献信息。- Distinct timescales of RNA regulators enable the construction of a genetic pulse generator
- (2019) Alexandra Westbrook et al. BIOTECHNOLOGY AND BIOENGINEERING
- Deconstructing cell-free extract preparation for in vitro activation of transcriptional genetic circuitry
- (2019) Adam Silverman et al. ACS Synthetic Biology
- A simple, robust, and low-cost method to produce the PURE cell-free system.
- (2019) Barbora Lavickova et al. ACS Synthetic Biology
- PERSIA for Direct Fluorescence Measurements of Transcription, Translation, and Enzyme Activity in Cell-Free Systems
- (2019) Scott Wick et al. ACS Synthetic Biology
- BioBitsTM Health: Classroom activities exploring engineering, biology, and human health with fluorescent readouts
- (2019) Jessica C. Stark et al. ACS Synthetic Biology
- Construction of Incoherent Feedforward Loop Circuits in a Cell-Free System and in Cells
- (2019) Shaobin Guo et al. ACS Synthetic Biology
- PLANT-Dx: A Molecular Diagnostic for Point-of-Use Detection of Plant Pathogens
- (2019) M. Verosloff et al. ACS Synthetic Biology
- Artificial photosynthetic cell producing energy for protein synthesis
- (2019) Samuel Berhanu et al. Nature Communications
- Characterization of the all‐ E. coli transcription‐translation system myTXTL by mass spectrometry
- (2019) David Garenne et al. RAPID COMMUNICATIONS IN MASS SPECTROMETRY
- Cell-free gene-regulatory network engineering with synthetic transcription factors
- (2019) Zoe Swank et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- A paper-based, cell-free biosensor system for the detection of heavy metals and date rape drugs
- (2019) Alexander Gräwe et al. PLoS One
- Spatiotemporal control of coacervate formation within liposomes
- (2019) Siddharth Deshpande et al. Nature Communications
- Plug-and-play metabolic transducers expand the chemical detection space of cell-free biosensors
- (2019) Peter L. Voyvodic et al. Nature Communications
- Metabolic Profiling of Escherichia coli-Based Cell-Free Expression Systems for Process Optimization
- (2019) April M. Miguez et al. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
- Multiplex transcriptional characterizations across diverse bacterial species using cell‐free systems
- (2019) Sung Sun Yim et al. Molecular Systems Biology
- Optimizing Cell-Free Biosensors to Monitor Enzymatic Production
- (2019) Amir Pandi et al. ACS Synthetic Biology
- Quantification of Interlaboratory Cell-Free Protein Synthesis Variability
- (2019) Stephanie D. Cole et al. ACS Synthetic Biology
- Accurate high-throughput screening based on digital protein synthesis in a massively parallel femtoliter droplet array
- (2019) Yi Zhang et al. Science Advances
- Point-of-care biomarker quantification enabled by sample-specific calibration
- (2019) Monica P. McNerney et al. Science Advances
- A cell-free biosynthesis platform for modular construction of protein glycosylation pathways
- (2019) Weston Kightlinger et al. Nature Communications
- Quantitative modeling of transcription and translation of an all-E. coli cell-free system
- (2019) Ryan Marshall et al. Scientific Reports
- Strand Displacement Probes Combined with Isothermal Nucleic Acid Amplification for Instrument-Free Detection from Complex Samples
- (2018) Elizabeth A. Phillips et al. ANALYTICAL CHEMISTRY
- Expanding the palette of Streptomyces -based cell-free protein synthesis systems with enhanced yields
- (2018) Jian Li et al. BIOCHEMICAL ENGINEERING JOURNAL
- Endotoxin-free E. coli -based cell-free protein synthesis: Pre-expression endotoxin removal approaches for on-demand cancer therapeutic production
- (2018) Kristen M. Wilding et al. Biotechnology Journal
- A Pressure Test to Make 10 Molecules in 90 Days: External Evaluation of Methods to Engineer Biology
- (2018) Arturo Casini et al. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
- Self-assembling functional programmable protein array for studying protein–protein interactions in malaria parasites
- (2018) Gabriela Arévalo-Pinzón et al. MALARIA JOURNAL
- Controlling cell-free metabolism through physiochemical perturbations
- (2018) Ashty S. Karim et al. METABOLIC ENGINEERING
- Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System
- (2018) Ryan Marshall et al. MOLECULAR CELL
- Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases
- (2018) Weston Kightlinger et al. Nature Chemical Biology
- The E. coli S30 lysate proteome: A prototype for cell-free protein production
- (2018) Daniel Foshag et al. New Biotechnology
- Oligonucleotide-mediated tRNA sequestration enables one-pot sense codon reassignment in vitro
- (2018) Zhenling Cui et al. NUCLEIC ACIDS RESEARCH
- Rapid acquisition and model-based analysis of cell-free transcription–translation reactions from nonmodel bacteria
- (2018) Simon J. Moore et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Field-deployable viral diagnostics using CRISPR-Cas13
- (2018) Cameron Myhrvold et al. SCIENCE
- Long-lived protein expression in hydrogel particles: towards artificial cells
- (2018) Xiaoyu Zhou et al. Chemical Science
- Cell-free prediction of protein expression costs for growing cells
- (2018) Olivier Borkowski et al. Nature Communications
- Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
- (2018) Thapakorn Jaroentomeechai et al. Nature Communications
- Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids
- (2018) Rey W. Martin et al. Nature Communications
- Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin
- (2018) Anne Zemella et al. Scientific Reports
- Rapid recombinant protein expression in cell-free extracts from human blood
- (2018) David Burgenson et al. Scientific Reports
- Cell-Free Optogenetic Gene Expression System
- (2018) Premkumar Jayaraman et al. ACS Synthetic Biology
- Engineering a Functional Small RNA Negative Autoregulation Network with Model-Guided Design
- (2018) Chelsea Y. Hu et al. ACS Synthetic Biology
- Quantitative Characterization of Translational Riboregulators Using an in Vitro Transcription–Translation System
- (2018) Anis Senoussi et al. ACS Synthetic Biology
- Sequence Specific Modeling of E. coli Cell-Free Protein Synthesis
- (2018) Michael Vilkhovoy et al. ACS Synthetic Biology
- Endotoxin-free E. coli -based cell-free protein synthesis: Pre-expression endotoxin removal approaches for on-demand cancer therapeutic production
- (2018) Kristen M. Wilding et al. Biotechnology Journal
- Cell-Free Protein Synthesis From Fast-Growing Vibrio natriegens
- (2018) Jurek Failmezger et al. Frontiers in Microbiology
- Point-of-care production of therapeutic proteins of good-manufacturing-practice quality
- (2018) Rajani Adiga et al. Nature Biomedical Engineering
- Systematic discovery of natural CRISPR-Cas12a inhibitors
- (2018) Kyle E. Watters et al. SCIENCE
- A low-cost paper-based synthetic biology platform for analyzing gut microbiota and host biomarkers
- (2018) Melissa K. Takahashi et al. Nature Communications
- Towards synthetic cells using peptide-based reaction compartments
- (2018) Kilian Vogele et al. Nature Communications
- Establishing a Cell-Free Vibrio natriegens Expression System
- (2018) Daniel J. Wiegand et al. ACS Synthetic Biology
- Establishing a High-Yielding Cell-Free Protein Synthesis Platform Derived from Vibrio natriegens
- (2018) Benjamin J. Des Soye et al. ACS Synthetic Biology
- BioBits™ Explorer: A modular synthetic biology education kit
- (2018) Ally Huang et al. Science Advances
- BioBits™ Bright: A fluorescent synthetic biology education kit
- (2018) Jessica C. Stark et al. Science Advances
- Artificial Gel-Based Organelles for Spatial Organization of Cell-Free Gene Expression Reactions
- (2018) Lukas Aufinger et al. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
- Cell-free supplement mixtures: Elucidating the history and biochemical utility of additives used to support in vitro protein synthesis in E. coli extract
- (2018) By Jared L. Dopp et al. BIOTECHNOLOGY ADVANCES
- Expanding biological applications using cell-free metabolic engineering: An overview
- (2018) James R. Swartz METABOLIC ENGINEERING
- Cell-free microcompartmentalised transcription–translation for the prototyping of synthetic communication networks
- (2018) Emilien Dubuc et al. CURRENT OPINION IN BIOTECHNOLOGY
- Preservation of protein expression systems at elevated temperatures for portable therapeutic production
- (2017) David K. Karig et al. Journal of the Royal Society Interface
- Cell-free extract based optimization of biomolecular circuits with droplet microfluidics
- (2017) Yutaka Hori et al. LAB ON A CHIP
- Multi-dimensional studies of synthetic genetic promoters enabled by microfluidic impact printing
- (2017) Jinzhen Fan et al. LAB ON A CHIP
- Digital-to-biological converter for on-demand production of biologics
- (2017) Kent S Boles et al. NATURE BIOTECHNOLOGY
- Experimentally Validated Model Enables Debottlenecking of in Vitro Protein Synthesis and Identifies a Control Shift under in Vivo Conditions
- (2017) Alexander Nieß et al. ACS Synthetic Biology
- Cell-Free and In Vivo Characterization of Lux, Las, and Rpa Quorum Activation Systems in E. coli
- (2017) Andrew D. Halleran et al. ACS Synthetic Biology
- Cell-Free Protein Synthesis Enhancement from Real-Time NMR Metabolite Kinetics: Redirecting Energy Fluxes in Hybrid RRL Systems
- (2017) Baptiste Panthu et al. ACS Synthetic Biology
- Rapid and Scalable Preparation of Bacterial Lysates for Cell-Free Gene Expression
- (2017) Andriy Didovyk et al. ACS Synthetic Biology
- Development of a CHO-Based Cell-Free Platform for Synthesis of Active Monoclonal Antibodies
- (2017) Rey W. Martin et al. ACS Synthetic Biology
- A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples
- (2017) Ke Yan Wen et al. ACS Synthetic Biology
- Streptomyces venezuelae TX-TL - a next generation cell-free synthetic biology tool
- (2017) Simon J. Moore et al. Biotechnology Journal
- Cell-free synthesis of functional antibodies using a coupled in vitro transcription-translation system based on CHO cell lysates
- (2017) M. Stech et al. Scientific Reports
- High-yield production of “difficult-to-express” proteins in a continuous exchange cell-free system based on CHO cell lysates
- (2017) Lena Thoring et al. Scientific Reports
- Portable, On-Demand Biomolecular Manufacturing
- (2016) Keith Pardee et al. CELL
- Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components
- (2016) Keith Pardee et al. CELL
- A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery
- (2016) Ashty S. Karim et al. METABOLIC ENGINEERING
- Development of a Bacillus subtilis cell-free transcription-translation system for prototyping regulatory elements
- (2016) Richard Kelwick et al. METABOLIC ENGINEERING
- Mass spectrometry-based absolute quantification reveals rhythmic variation of mouse circadian clock proteins
- (2016) Ryohei Narumi et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology
- (2016) Jonathan Garamella et al. ACS Synthetic Biology
- Cell-Free Mixing of Escherichia coli Crude Extracts to Prototype and Rationally Engineer High-Titer Mevalonate Synthesis
- (2016) Quentin M. Dudley et al. ACS Synthetic Biology
- LyophilizedEscherichia coli-based cell-free systems for robust, high-density, long-term storage
- (2016) Mark Thomas Smith et al. BIOTECHNIQUES
- Robust Strand Exchange Reactions for the Sequence-Specific, Real-Time Detection of Nucleic Acid Amplicons
- (2015) Yu Sherry Jiang et al. ANALYTICAL CHEMISTRY
- Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system
- (2015) Amin S. M. Salehi et al. Biotechnology Journal
- A cell-free expression and purification process for rapid production of protein biologics
- (2015) Challise J. Sullivan et al. Biotechnology Journal
- Improving Cell-Free Protein Synthesis through Genome Engineering ofEscherichia coliLacking Release Factor 1
- (2015) Seok Hoon Hong et al. CHEMBIOCHEM
- In vitro gene expression within membrane-free coacervate protocells
- (2015) T.-Y. Dora. Tang et al. CHEMICAL COMMUNICATIONS
- Lysate of engineered Escherichia coli supports high-level conversion of glucose to 2,3-butanediol
- (2015) Jennifer E. Kay et al. METABOLIC ENGINEERING
- Characterizing and prototyping genetic networks with cell-free transcription–translation reactions
- (2015) Melissa K. Takahashi et al. METHODS
- Automated physics-based design of synthetic riboswitches from diverse RNA aptamers
- (2015) Amin Espah Borujeni et al. NUCLEIC ACIDS RESEARCH
- Toward Microfluidic Reactors for Cell-Free Protein Synthesis at the Point-of-Care
- (2015) Andrea C. Timm et al. Small
- Robust production of recombinant phosphoproteins using cell-free protein synthesis
- (2015) Javin P. Oza et al. Nature Communications
- High-throughput preparation methods of crude extract for robust cell-free protein synthesis
- (2015) Yong-Chan Kwon et al. Scientific Reports
- Engineering Transcriptional Regulator Effector Specificity Using Computational Design and In Vitro Rapid Prototyping: Developing a Vanillin Sensor
- (2015) Emmanuel L. C. de los Santos et al. ACS Synthetic Biology
- Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system
- (2015) Amin S. M. Salehi et al. Biotechnology Journal
- A cell-free expression and purification process for rapid production of protein biologics
- (2015) Challise J. Sullivan et al. Biotechnology Journal
- Rapid cell-free forward engineering of novel genetic ring oscillators
- (2015) Henrike Niederholtmeyer et al. eLife
- Synthesis of 2.3 mg/ml of protein with an all Escherichia coli cell-free transcription–translation system
- (2014) Filippo Caschera et al. BIOCHIMIE
- Production of Site-Specific Antibody–Drug Conjugates Using Optimized Non-Natural Amino Acids in a Cell-Free Expression System
- (2014) Erik S. Zimmerman et al. BIOCONJUGATE CHEMISTRY
- Toehold Switches: De-Novo-Designed Regulators of Gene Expression
- (2014) Alexander A. Green et al. CELL
- Paper-Based Synthetic Gene Networks
- (2014) Keith Pardee et al. CELL
- DNA-functionalized hydrogels for confined membrane-free in vitro transcription/translation
- (2014) J. Thiele et al. LAB ON A CHIP
- Engineering toward a bacterial “endoplasmic reticulum” for the rapid expression of immunoglobulin proteins
- (2014) Dan Groff et al. mAbs
- Programmable on-chip DNA compartments as artificial cells
- (2014) E. Karzbrun et al. SCIENCE
- Gene Circuit Performance Characterization and Resource Usage in a Cell-Free “Breadboard”
- (2014) Dan Siegal-Gaskins et al. ACS Synthetic Biology
- Stochasticity in Gene Expression in a Cell-Sized Compartment
- (2014) Kazuya Nishimura et al. ACS Synthetic Biology
- Rapidly Characterizing the Fast Dynamics of RNA Genetic Circuitry with Cell-Free Transcription–Translation (TX-TL) Systems
- (2014) Melissa K. Takahashi et al. ACS Synthetic Biology
- Optimized extract preparation methods and reaction conditions for improved yeast cell-free protein synthesis
- (2013) C. Eric Hodgman et al. BIOTECHNOLOGY AND BIOENGINEERING
- Broadening Horizons and Teaching Basic Biology Through Cell-Free Synthesis of Green Fluorescent Protein in a High School Laboratory Course
- (2013) Cem Albayrak et al. Journal of Science Education and Technology
- Cell-free unnatural amino acid incorporation with alternative energy systems and linear expression templates
- (2013) Prashanta Shrestha et al. New Biotechnology
- Cell-free co-production of an orthogonal transfer RNA activates efficient site-specific non-natural amino acid incorporation
- (2013) Cem Albayrak et al. NUCLEIC ACIDS RESEARCH
- Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology
- (2013) James Chappell et al. NUCLEIC ACIDS RESEARCH
- Implementation of cell-free biological networks at steady state
- (2013) H. Niederholtmeyer et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Enhanced transcription rates in membrane-free protocells formed by coacervation of cell lysate
- (2013) E. Sokolova et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Linear DNA for Rapid Prototyping of Synthetic Biological Circuits in an Escherichia coli Based TX-TL Cell-Free System
- (2013) Zachary Z. Sun et al. ACS Synthetic Biology
- Cell-free Protein Synthesis from a Release Factor 1 Deficient Escherichia coli Activates Efficient and Multiple Site-specific Nonstandard Amino Acid Incorporation
- (2013) Seok Hoon Hong et al. ACS Synthetic Biology
- Adapting Enzyme-Free DNA Circuits to the Detection of Loop-Mediated Isothermal Amplification Reactions
- (2012) Bingling Li et al. ANALYTICAL CHEMISTRY
- In silico assessment of cell-free systems
- (2012) Matthias Bujara et al. BIOTECHNOLOGY AND BIOENGINEERING
- A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution
- (2012) Ali Fallah-Araghi et al. LAB ON A CHIP
- Aglycosylated antibodies and antibody fragments produced in a scalable in vitro transcription-translation system
- (2012) Gang Yin et al. mAbs
- An engineered eukaryotic protein glycosylation pathway in Escherichia coli
- (2012) Juan D Valderrama-Rincon et al. Nature Chemical Biology
- Cell-free protein synthesis and assembly on a biochip
- (2012) Yael Heyman et al. Nature Nanotechnology
- Massively parallel measurements of molecular interaction kinetics on a microfluidic platform
- (2012) M. Geertz et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Real-Time mRNA Measurement during an in Vitro Transcription and Translation Reaction Using Binary Probes
- (2012) Henrike Niederholtmeyer et al. ACS Synthetic Biology
- Genome Replication, Synthesis, and Assembly of the Bacteriophage T7 in a Single Cell-Free Reaction
- (2012) Jonghyeon Shin et al. ACS Synthetic Biology
- Cell-free protein synthesis: Applications come of age
- (2011) Erik D. Carlson et al. BIOTECHNOLOGY ADVANCES
- Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines
- (2011) James F. Zawada et al. BIOTECHNOLOGY AND BIOENGINEERING
- Cell-free synthesis of a functional G protein-coupled receptor complexed with nanometer scale bilayer discs
- (2011) Jian-Ping Yang et al. BMC BIOTECHNOLOGY
- Fabrication optimization of a miniaturized array device for cell-free protein synthesis
- (2011) Ruba Khnouf et al. ELECTROPHORESIS
- A prokaryote-based cell-free translation system that efficiently synthesizes glycoproteins
- (2011) Cassandra Guarino et al. GLYCOBIOLOGY
- Cell-free synthetic biology: Thinking outside the cell
- (2011) C. Eric Hodgman et al. METABOLIC ENGINEERING
- Optimization of a blueprint for in vitro glycolysis by metabolic real-time analysis
- (2011) Matthias Bujara et al. Nature Chemical Biology
- Expression optimization and synthetic gene networks in cell-free systems
- (2011) David K. Karig et al. NUCLEIC ACIDS RESEARCH
- Coarse-Grained Dynamics of Protein Synthesis in a Cell-Free System
- (2011) Eyal Karzbrun et al. PHYSICAL REVIEW LETTERS
- An E. coli Cell-Free Expression Toolbox: Application to Synthetic Gene Circuits and Artificial Cells
- (2011) Jonghyeon Shin et al. ACS Synthetic Biology
- Miniaturized fluid array for high-throughput protein expression
- (2010) Ruba Khnouf et al. BIOTECHNOLOGY PROGRESS
- Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70
- (2010) Jonghyeon Shin et al. Journal of Biological Engineering
- Synthetic translational regulation by an L7Ae–kink-turn RNP switch
- (2009) Hirohide Saito et al. Nature Chemical Biology
- Rapid Screening of Quorum-Sensing Signal N-Acyl Homoserine Lactones by an In Vitro Cell-Free Assay
- (2008) T. Kawaguchi et al. APPLIED AND ENVIRONMENTAL MICROBIOLOGY
- High yield cell-free production of integral membrane proteins without refolding or detergents
- (2008) Jessica J. Wuu et al. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
- Tuning the expression level of recombinant proteins by modulating mRNA stability in a cell-free protein synthesis system
- (2008) Jin-Ho Ahn et al. BIOTECHNOLOGY AND BIOENGINEERING
- An Integrated Device for Monitoring Time-Dependent in vitro Expression From Single Genes in Picolitre Droplets
- (2008) Fabienne Courtois et al. CHEMBIOCHEM
- Cell-free protein expression in a microchannel array with passive pumping
- (2008) Ruba Khnouf et al. LAB ON A CHIP
- An integrated cell-free metabolic platform for protein production and synthetic biology
- (2008) Michael C Jewett et al. Molecular Systems Biology
- Printing protein arrays from DNA arrays
- (2008) Mingyue He et al. NATURE METHODS
- Escherichia coli-based cell-free synthesis of virus-like particles
- (2007) Bradley C. Bundy et al. BIOTECHNOLOGY AND BIOENGINEERING
- An Economical Method for Cell-Free Protein Synthesis using Glucose and Nucleoside Monophosphates
- (2005) Kara A. Calhoun et al. BIOTECHNOLOGY PROGRESS
- Streamlining Escherichia Coli S30 Extract Preparation for Economical Cell-Free Protein Synthesis
- (2005) David V. Liu et al. BIOTECHNOLOGY PROGRESS
Publish scientific posters with Peeref
Peeref publishes scientific posters from all research disciplines. Our Diamond Open Access policy means free access to content and no publication fees for authors.
Learn MoreBecome a Peeref-certified reviewer
The Peeref Institute provides free reviewer training that teaches the core competencies of the academic peer review process.
Get Started