Article
Optics
Ning Ma, Changfeng Wu, Zihui Meng
Summary: This review discusses various methods for in vivo imaging and tracking of exosomes, highlighting the importance of understanding exosomal behaviors in vivo for their clinical application. Efforts in labeling and in vivo imaging techniques are crucial for expanding knowledge of exosomes and promoting their use in disease theranostics.
JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Maria Isabel Gonzalez, Mario Gonzalez-Arjona, Ana Santos-Coquillat, Javier Vaquero, Elena Vazquez-Ogando, Antonio de Molina, Hector Peinado, Manuel Desco, Beatriz Salinas
Summary: A novel chemical approach for robust attachment of commercial fluorescent dyes to exosome surface was reported, demonstrating no alteration of original physicochemical properties. Successful validation of the methodology in cell cultures and healthy mice indicates its potential for in vitro and in vivo applications. The fluorescently labeled exosomes were effectively visualized with optical imaging.
Article
Biochemistry & Molecular Biology
Tianqi Liu, Zhenlin Li, Xiaodong Li, Ruiting Zhao, Xinhua Wei, Zixin Wang, Sherman Xuegang Xin
Summary: Exosomes play a significant role in promoting tumor progression, and magnetic resonance imaging (MRI) is an ideal technique to visualize their dynamics. A novel self-label method was proposed for imaging labeled exosomes of tumor cells in vivo through MRI.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2022)
Review
Immunology
Lifei Liang, Xiaoqing Xu, Jiawei Li, Cheng Yang
Summary: This review summarizes and discusses the bidirectional regulation between miRNAs and MDSCs in the tumor microenvironment, revealing the impact of miRNAs and MDSCs on tumor immune escape and metastasis.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Endocrinology & Metabolism
Dian R. Arifin, Jeff W. M. Bulte
Summary: Pancreatic islet transplantation has the potential to provide life-long blood glucose management for diabetic patients, with imaging technology playing a crucial role in monitoring and evaluating the success of cell implants in vivo. Various imaging modalities are being developed to improve the precision and efficacy of islet therapy.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biochemical Research Methods
Qin Zhang, Dennis K. Jeppesen, James N. Higginbotham, Jeffrey L. Franklin, Robert J. Coffey
Summary: There is increasing recognition of the diverse nature of extracellular vesicles (EVs). Moreover, two nonvesicular extracellular nanoparticles (NVEPs), exomeres and supermeres, have been identified as carriers of cargo previously attributed to EVs. This article provides a detailed protocol for the sequential isolation of distinct populations of EVs and NVEPs using methods such as ultracentrifugation, filtration, and density-gradient fractionation. The protocol offers advantages in terms of purity, reproducibility, and applicability to various sample sources. It has the potential to advance translational research and deepen our understanding of EV and NVEP biogenesis and function.
Article
Chemistry, Multidisciplinary
Zichao Luo, Dehong Hu, Duyang Gao, Zhigao Yi, Hairong Zheng, Zonghai Sheng, Xiaogang Liu
Summary: This study introduces the use of bioorthogonal nanoprobes with high tumor-targeting specificity for in vivo NIR-IIb luminescence imaging and MRI, improving imaging quality and reducing toxicity.
ADVANCED MATERIALS
(2021)
Article
Nanoscience & Nanotechnology
Juan Manuel Arango, Daniel Padro, Jorge Blanco, Sonia Lopez-Fernandez, Pilar Castellnou, Palmira Villa-Valverde, Jesus Ruiz-Cabello, Abraham Martin, Monica Carril
Summary: A novel fluorine labeling strategy was proposed in this study, which successfully synthesized potential fluorinated nanoparticles through conjugation of small fluorinated building blocks with gold nanoparticles. These nanoparticles exhibited potential applications in biological imaging in vivo using F-19 magnetic resonance spectroscopy in mice.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Review
Cardiac & Cardiovascular Systems
Amankeldi A. Salybekov, Aidyn D. Kunikeyev, Shuzo Kobayashi, Takayuki Asahara
Summary: Extracellular vesicles (EVs) secreted by nucleated cells, especially endothelial progenitor cell-derived EVs (EPC-EVs), have shown beneficial effects in various diseases such as cardiovascular and lung disorders, accumulating at injury sites with angiogenic and restorative effects surpassing their ancestors. This review article focuses on the biogenesis, delivery routes, and long-term storage methods of EPC-EVs, highlighting their anti-inflammatory, angiogenic, and tissue protection effects in diseases, and discussing the therapeutic potential of EPC-EVs in clinical applications.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Evgeniya E. Burkova, Sergey E. Sedykh, Georgy A. Nevinsky
Summary: Exosomes are small nanovesicles that play a crucial role in intercellular communication and are involved in transferring various bioactive substances. During pregnancy, the placenta releases exosomes into the maternal circulation, affecting cell behavior. Their composition and concentration can impact fetal development and pregnancy outcomes. Research on exosome composition changes may lead to early diagnosis methods for pregnancy pathologies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Risa Azuma, Toshihide Yamasaki, Kohei Sano, Masayuki Munekane, Yuta Matsuoka, Ken-ichi Yamada, Takahiro Mukai
Summary: A radioiodinated nitroxide derivative with resistance to bioreduction was successfully developed for in vivo detection of lipid radicals, displaying high stability and reactivity towards lipid radicals. The compound showed significant uptake by cells under lipid radical-producing conditions and accumulated more in organs where lipid peroxidation was promoted, making it a candidate probe for in vivo detection of lipid radicals.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Review
Chemistry, Inorganic & Nuclear
Lukasz Kiraga, Paulina Kucharzewska, Stephen Paisey, Lukasz Cheda, Anita Domanska, Zbigniew Rogulski, Tomasz P. Rygiel, Alberto Boffi, Magdalena Krol
Summary: This review paper discusses the pivotal role of nuclear imaging techniques in evaluating the effectiveness of immune cell-based therapies, focusing on tracking cells and their ability to home to the target tissue. It compares different types of radiotracers, outlines various ways of immune cell labeling, and provides the latest examples for the use of nuclear imaging techniques in both preclinical studies and clinical settings.
COORDINATION CHEMISTRY REVIEWS
(2021)
Article
Chemistry, Multidisciplinary
Lin Zhu, Yuanfeng Xu, Xinyu Wei, Haoting Lin, Mengjiao Huang, Bingqian Lin, Yanling Song, Chaoyong Yang
Summary: The study developed a dual labeling strategy to visualize the glycosylation of specific proteins on exosomes, enabling in situ visualization and biological function study. Exosomal PD-L1 glycosylation was confirmed to be required in interaction with PD-1 and participated in inhibiting CD8(+) T cell proliferation, offering a powerful tool for exosomal glycoproteomics research.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Gastroenterology & Hepatology
Liyuan Lin, Jia Song, Jian Li, Xiaolei Zuo, Hong Wei, Chaoyong Yang, Wei Wang
Summary: Studying unculturable bacteria in the human gut is challenging and the lack of knowledge hinders further understanding of the microbiota. Through the integrative use of STAMP and FISH, growth and division of gut bacteria can be characterized in an animal model. The findings reveal that many bacterial species in the human microbiota are significantly shorter, and an imaging database for summarizing bacterial imaging has been established.
Review
Chemistry, Multidisciplinary
Yingying Jing, Chenshuang Zhang, Bin Yu, Danying Lin, Junle Qu
Summary: Super-resolution microscopy has significantly improved the resolution for visualizing biological activities in cells, but observing cell structures and dynamics in live cells and organisms remains challenging. Recent advancements in SRM have shown promise in live imaging and offer new opportunities for studying biological processes at high spatiotemporal resolution.
FRONTIERS IN CHEMISTRY
(2021)
Article
Oncology
Bhagelu R. Achyut, Hao Zhang, Kartik Angara, Nahid F. Mivechi, Ali S. Arbab, Lan Ko
Article
Multidisciplinary Sciences
Kumar Vaibhav, Molly Braun, Katelyn Alverson, Hesam Khodadadi, Ammar Kutiyanawalla, Ayobami Ward, Christopher Banerjee, Tyler Sparks, Aneeq Malik, Mohammad H. Rashid, Mohammad Badruzzaman Khan, Michael F. Waters, David C. Hesss, Ali S. Arbab, John R. Vender, Nasrul Hoda, Babak Baban, Krishnan M. Dhandapani
Article
Oncology
Mohammad H. Rashid, Thaiz F. Borin, Roxan Ara, Raziye Piranlioglu, Bhagelu R. Achyut, Hasan Korkaya, Yutao Liu, Ali S. Arbab
Summary: MDSC-derived exosomes carry proteins that play a crucial role in tumor growth, invasion, angiogenesis, and immunomodulation. They are capable of hyper-activating or exhausting CD8 T-cells and inducing activation-induced cell death. In vivo experiments showed a depletion of CD8 T-cells and a reduction in pro-inflammatory M1-macrophages in the spleen after treatment with MDSC exosomes.
Article
Multidisciplinary Sciences
Sehar Ali, Thaiz F. Borin, Raziye Piranlioglu, Roxan Ara, Iryna Lebedyeva, Kartik Angara, Bhagelu R. Achyut, Ali Syed Arbab, Mohammad H. Rashid
Summary: This study aimed to investigate the effects of different treatments on TME-associated cells in GBM patients, and the results showed that TME-targeting therapy improved the survival of animals. It is suggested to revisit current GBM therapies and adopt a sequential strategy targeting TME-associated cells.
Article
Immunology
Mahmoud Elashiry, Ranya Elsayed, Mohamed M. Elashiry, Mohammad H. Rashid, Roxan Ara, Ali S. Arbab, Ahmed R. Elawady, Mark Hamrick, Yutao Liu, Wenbo Zhi, Rudolf Lucas, Jose Vazquez, Christopher W. Cutler
Summary: Dendritic cell-derived exosomes are being investigated for their potential in treating inflammatory diseases, with the ability to customize immune functions and utilize TGF beta 1 signaling for downstream effects. Proteomic analysis revealed their biodistribution in tissues and their role in regulating immune responses in acceptor cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nicklaus A. Sparrow, Faizan Anwar, Ambart E. Covarrubias, Padmesh S. Rajput, Mohammad Harun Rashid, Peyton L. Nisson, Michael M. Gezalian, Shahed Toossi, Maranatha O. Ayodele, S. Ananth Karumanchi, E. Wesley Ely, Shouri Lahiri
Summary: Mechanical ventilation is a risk factor for cognitive impairment, with upregulation of IL-6 potentially playing a role. Inhibiting systemic IL-6 could mitigate neuropathological effects associated with cognitive impairment, providing a potential novel neuroprotective strategy worth further investigation.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Ahmet Alptekin, Mohammad B. Khan, Roxan Ara, Mohammad H. Rashid, Fengchong Kong, Mahrima Parvin, Joseph A. Frank, Rajiv Chopra, Krishnan Dhandapani, Ali S. Arbab
Summary: Exosomes have been shown to carry important biomolecules and can be delivered to stroke areas using pulsed focused ultrasound, but the administration of nanobubbles has detrimental effects on brain structures.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2021)
Article
Immunology
Mohammad Harun Rashid, Nicklaus A. Sparrow, Faizan Anwar, Gena Guidry, Ambart E. Covarrubias, Haoming Pang, Chandrakumar Bogguri, S. Ananth Karumanchi, Shouri Lahiri
Summary: The study identified the crucial role of IL-6 in mediating delirium-like phenotypes in a mouse model of UTI, with systemic anti-IL-6 treatment showing complete reversal of neural functional impairments induced by UTI. These findings provide pre-clinical justification for further clinical investigations on the use of IL-6 inhibitors to treat UTI-induced delirium.
JOURNAL OF NEUROINFLAMMATION
(2021)
Review
Neurosciences
Elliot Hogg, Samuel Frank, Jillian Oft, Brian Benway, Mohammad Harun Rashid, Shouri Lahiri
Summary: Urinary tract infection (UTI) is a common precipitant of acute neurological deterioration in patients with Parkinson's disease (PD). It can lead to delirium, functional decline, falls, and hospitalization. PD patients are at an increased risk of UTI due to various clinical features, such as autonomic dysfunction and altered urodynamics, frailty and cognitive impairment, and the need for bladder catheterization. Sepsis resulting from untreated or undertreated UTI is a feared consequence and a major cause of morbidity in PD. Immune-mediated brain injury may be involved in the pathogenesis of UTI-induced deterioration of PD symptoms. Existing strategies for preventing UTI in PD patients include the use of topical estrogen, prophylactic supplements, antibiotic bladder irrigation, clean catheterization techniques, and prophylactic oral antibiotics.
JOURNAL OF PARKINSONS DISEASE
(2022)
Article
Chemistry, Multidisciplinary
Rameshwar Patil, Tao Sun, Mohammad Harun Rashid, Liron L. Israel, Arshia Ramesh, Saya Davani, Keith L. Black, Alexander Ljubimov, Eggehard Holler, Julia Y. Ljubimova
Summary: This study aimed to develop multifunctional nanomedicines for treating glioblastoma using combination therapy targeting multiple molecular markers. By utilizing multifunctional nanopolymers, the growth of GBM cells was effectively inhibited, significantly increasing the survival rate of tumor-bearing animals.
Article
Oncology
Mujibur Khan, Md Mahmudul Hasan, Anne Barnett, Raziye Piranlioglu, Mohammad Rashid, Ahmet Alptekin, Ali Arbab
Summary: A novel coaxial electrospraying technique was developed to produce multi-cancer drug nanocapsules, which showed prolonged and sustained drug release patterns in vitro and in vivo studies. The nanocapsules containing multiple drugs were effective in controlling tumor cell growth and increasing survival rates in animals with breast cancers.
CANCER NANOTECHNOLOGY
(2022)
Meeting Abstract
Clinical Neurology
Mohammad B. Khan, Shahneela Siddiqui, Amna Rehman, Rolf A. Blauenfeldt, Haroon Alam, Muhammad F. Shaikh, Abhinav Sharma, Kumar Vaibhav, Molly Braun, Bhagelu R. Achyut, Mohammad H. Rashid, Hesamoldin Khodadadi, Babak Baban, Krishnan M. Dhandapani, David C. Hess
Article
Pharmacology & Pharmacy
Mohammad Harun Rashid, Thaiz F. Borin, Roxan Ara, Ahmet Alptekin, Yutao Liu, Ali S. Arbab
ADVANCED THERAPEUTICS
(2020)
Article
Multidisciplinary Sciences
Kartik Angara, Emily Ling-Lin Pai, Stephanie M. Bilinovich, April M. Stafford, Julie T. Nguyen, Katie X. Li, Anirban Paul, John L. Rubenstein, Daniel Vogt
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Nanoscience & Nanotechnology
Daniela Rubanova, Svitlana Skoroplyas, Alena Libanska, Eva Randarova, Josef Bryja, Michaela Chorvatova, Tomas Etrych, Lukas Kubala
Summary: This study explored the use of nanocarriers to deliver dexamethasone for treating rheumatoid arthritis. The results showed that this method increased the accumulation of dexamethasone in the arthritic area, had a dose-dependent healing effect, and reduced the accumulation of the RA mediator RANKL. This study provides a feasible strategy for improving rheumatoid arthritis therapy.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2024)
Article
Nanoscience & Nanotechnology
Thanpisit Lomphithak, Apiwit Sae-Fung, Simone Sprio, Anna Tampieri, Siriporn Jitkaew, Bengt Fadeel
Summary: In this study, iron-doped hydroxyapatite (FeHA) was evaluated as a potential nanomedicine approach for treating PDAC. The results showed that FeHA, in combination with a sublethal dose of the GPX4 inhibitor RSL3, could trigger ferroptosis in KRAS mutant PANC-1 cells while sparing normal human cells. The induction of ferroptosis by FeHA plus RSL3 was found to be dependent on the metalloreductase STEAP3 in PDAC cells.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2024)
Review
Nanoscience & Nanotechnology
Marco Felipe Salas-Orozco, Ana Cecilia Lorenzo-Leal, Idania de Alba Montero, Nuria Patino Marin, Miguel Angel Casillas Santana, Horacio Bach
Summary: The emergence of antibiotic-resistant bacteria in severe infections is a growing concern, particularly in hospital environments. Metal-based nanomaterials have shown potential as an alternative to combat these bacteria, but there is a risk of bacterial resistance and environmental accumulation.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2024)