Article
Biophysics
Jayeon Song, Hyo Yong Kim, Soohyun Kim, Yujin Jung, Hyun Gyu Park
Summary: A novel technology, SP-HAMP, is described for target nucleic acid detection under isothermal conditions. By designing a unique hairpin probe, it is possible to detect target DNA with outstanding discrimination capability using only a single primer, without the need for complex multiple primers.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Chemistry, Analytical
Poramin Boonbanjong, Kiatnida Treerattrakoon, Wassa Waiwinya, Piyawat Pitikultham, Deanpen Japrung
Summary: Isothermal amplification (IA) is a nucleic acid amplification technology that has significantly contributed to the healthcare system by improving disease diagnosis through higher sensitivity, specificity, and rapidity. Certain IA techniques provide a rapid, sensitive, specific, and simple method for disease diagnosis. However, not all IA techniques have been widely used in clinical applications due to the influence of different biomarkers and sample types on the IA system.
Article
Chemistry, Analytical
Daehan Nam, Seokjoon Kim, Jung Ho Kim, Seungjin Lee, Daneub Kim, Jinseo Son, Doyeon Kim, Byung Seok Cha, Eun Sung Lee, Ki Soo Park
Summary: Loop-mediated isothermal amplification (LAMP) is a widely used molecular diagnostics technology. This study introduces a new version of LAMP, called low-temperature LAMP, which operates at 37 degrees C for on-site diagnosis. The low-temperature LAMP differs from conventional LAMP in terms of MgSO4 and dNTPs concentrations, as well as the lengths of DNA probes. Under optimal conditions, low-temperature LAMP shows comparable amplification efficiency to conventional LAMP. Additionally, the method enables the detection of miR-21 with high sensitivity and selectivity at 37 degrees C, without requiring expensive DNA modifications or special additives.
Article
Multidisciplinary Sciences
Radhika Biyani, Kirti Sharma, Kenji Kojima, Madhu Biyani, Vishnu Sharma, Tarun Kumawat, Kevin Maafu Juma, Itaru Yanagihara, Shinsuke Fujiwara, Eiichi Kodama, Yuzuru Takamura, Masahiro Takagi, Kiyoshi Yasukawa, Manish Biyani
Summary: This new isothermal nucleic acid amplification assay, called RICCA, allows for rapid and direct detection of viruses with low viral loads. It has shown high sensitivity in detecting RNA viruses directly from saliva samples and synthetic templates of SARS-CoV-2. The assay can be used in real-time point-of-care testing and has a total assay time of 15-30 minutes for detecting heat-inactivated SARS-CoV-2 virus in human saliva.
SCIENTIFIC REPORTS
(2021)
Article
Nanoscience & Nanotechnology
Shiyao Wang, Ailin Qin, Li Yin Chau, Eunice W. T. Fok, Mei Yue Choy, Christopher J. Brackman, Gilman K. H. Siu, Chien-Ling Huang, Shea Ping Yip, Thomas M. H. Lee
Summary: Loop-mediated isothermal amplification (LAMP) has received substantial attention for decentralized nucleic acid testing due to its simple temperature control and short assay time. However, the existing amplification result reporter probes have limitations. This study demonstrates a new reporter probe based on CdSeS/ ZnS quantum dots with superior photostability, enabling single-copy sensitivity. The amine-QD-based one-step LAMP assay advances the field of QD-based nucleic acid amplification assays by achieving compatibility and universality.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Nanoscience & Nanotechnology
Shiyao Wang, Ailin Qin, Li Yin Chau, Eunice W. T. Fok, Mei Yue Choy, Christopher J. Brackman, Gilman K. H. Siu, Chien-Ling Huang, Shea Ping Yip, Thomas M. H. Lee
Summary: In this study, CdSeS/ZnS quantum dots with surface modification of cysteamine were used as a new reporter probe for LAMP, enabling single-copy sensitivity. The positively charged amine-QDs remained dispersed in negative LAMP samples, while they became precipitated in positive LAMP samples. This method has the advantages of compatibility and universality.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Siwenjie Qian, Yanju Chen, Cheng Peng, Xiaofu Wang, Hui Wu, Yang Che, Huanying Wang, Junfeng Xu, Jian Wu
Summary: This article introduces a simple and cost-effective method for detecting African swine fever virus (ASFV). The method utilizes filter paper to purify nucleic acids from swine blood and employs CRISPR/Cas12a-mediated loop-mediated isothermal amplification (LAMP) reaction to detect ASFV. The method does not require complex laboratory conditions, and has the advantages of rapid and visualized detection, making it suitable for on-site application.
Article
Chemistry, Analytical
Rui Mao, Tianzuo Wang, Yue Zhao, Xinyao Wu, Shun Zhang, Ting Cai
Summary: The article presents a novel isothermal amplification method called closed dumbbell mediated isothermal amplification (CDA) for nucleic acids. The method offers the advantages of simple primer design, high amplification efficiency, and successful detection of virus genes and bacterial pathogens. Real-time and colorimetric CDA detection methods have also been developed and proven effective.
Review
Food Science & Technology
Jie Zhou, Teng-Yu Wang, Zhi Lan, Han-Jie Yang, Xing-Jian Ye, Rui Min, Zhao-Hui Wang, Qing Huang, Jing Cao, Yu-E Gao, Wen-Long Wang, Xiu-Lan Sun, Yi Zhang
Summary: Foodborne microbial contamination (FMC) is the primary cause of food poisoning and illness. Isothermal amplification methods with functional nucleic acids (FNAs) have enabled the detection of mycotoxins, making biosensors for FMC more sensitive, cost-effective, and less reliant on nanomaterials for signal output.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Analytical
Shengjun Bu, Qianyu Zhou, Liming Liu, Chunyang Zhou, Zhuo Hao, Jiayu Wan, Chunying Pang
Summary: The present study describes a novel technology called TPIA that enables target nucleic acid detection. By using specific design strategy and fluorescence techniques, this system provides a versatile tool with great potential for biomedical research and clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Jacob Amos Hambalek, Janay Elise Kong, Calvin Brown, Hector Enrique Munoz, Thomas Horn, Michael Bogumil, Eleni Quick, Aydogan Ozcan, Dino Di Carlo
Summary: The study demonstrates a simple workflow for detecting methylated CpG dinucleotides in DNA samples using restriction enzyme digestion and loop-mediated isothermal amplification. Additionally, a cost-effective portable fluorescence reader is shown to transduce fluorescence signals associated with DNA amplification.
Article
Biotechnology & Applied Microbiology
Rui Mao, Xinyao Wu, Qing Miao, Ting Cai
Summary: Nucleic acid-based assays, such as Loop-mediated isothermal amplification (LAMP), have been widely used in clinical diagnostics, food safety, and environmental monitoring. A novel method called asymmetric stem-loop-mediated isothermal amplification (ASLAMP) was developed to overcome the challenges of specific primer design and non-specific amplification associated with LAMP. ASLAMP showed promising results in detecting the H1N1 gene fragment with simple primer design, short target sequence, and high amplification efficiency, offering potential for point-of-care DNA isothermal amplification.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Seokhwan Kim, Jung Ho Kim, Seokjoon Kim, Jung Soo Park, Byung Seok Cha, Eun Sung Lee, Jinjoo Han, Jiye Shin, Youngjun Jang, Ki Soo Park
Summary: A loop-mediated isothermal amplification-based nucleic acid lateral flow assay (LAMP-NALFA) system was developed for specific and multiplex detection of genetic markers at a low cost. The system demonstrated high selectivity in discriminating target amplification products and non-specific amplification products, and showed good practical applicability in different samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Food Science & Technology
Yu Liu, Yilin Zhang, Man Wang, Guoqiang Shi, Zhenguo Dong, Lei Ye, Lei Shi
Summary: In this study, we developed and validated an improved loop-mediated isothermal amplification (LAMP) method combined with a TaqMan probe for detecting Mycoplasma gallinis (MG). The LAMP primer set was screened using a test plasmid constructed with a conserved sequence of the specific gene vlhA of MG. The loop primer modified by fluorescent/quenching dyes was used as the TaqMan probe to establish the LAMP-TaqMan detection method. The results showed that LAMP-TaqMan had good specificity in detecting MG nucleic acids, with a low detection limit and a high detection rate.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Hongbo Shan, Yang Wang, Tao Wu, Binwu Ying, Gaolian Xu
Summary: Asymmetric hairpins mediated amplification (HMA) is a novel isothermal amplification method developed for the diagnosis of Mycobacterium Tuberculosis (MTB). It has high sensitivity, specificity, and direct visualization, making it suitable for point-of-care (POC) diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Endocrinology & Metabolism
Brian T. Steffen, Weihong Tang, Pamela L. Lutsey, Ryan T. Demmer, Elizabeth Selvin, Kunihiro Matsushita, Alanna C. Morrison, Weihua Guan, Mary R. Rooney, Faye L. Norby, Nathan Pankratz, David Couper, James S. Pankow
Summary: Proteomics analysis identified plasma proteins associated with the risk of type 2 diabetes. These proteins may serve as potential targets for intervention or pharmacological approaches to prevent, reverse, or slow down the progression of type 2 diabetes.
Article
Biochemistry & Molecular Biology
Kristen J. Polinski, Sonia L. Robinson, Diane L. Putnick, Weihua Guan, Jessica L. Gleason, Sunni L. Mumford, Rajeshwari Sundaram, Pauline Mendola, Stephanie London, Edwina H. Yeung
Summary: DNAm GA may better reflect biological maturation and is associated with decreased odds of developmental delay in early childhood. However, GAA is not associated with developmental delays.
HUMAN MOLECULAR GENETICS
(2023)
Article
Ophthalmology
Natalie L. Weir, Weihua Guan, Amy B. Karger, Barbara E. K. Klein, Stacy M. Meuer, Mary Frances Cotch, Xiuqing Guo, Xiaohui Li, Jingyi Tan, Pauline Genter, Yii-Der Ida Chen, Jerome I. Rotter, Eli Ipp, Michael Y. Tsai
Summary: The purpose of this study was to determine if omega-3 polyunsaturated fatty acids, specifically docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), are associated with decreased risk and severity of retinopathy in individuals with type 2 diabetes. The results showed an inverse association between DHA and the presence and severity of diabetic retinopathy. Therefore, increasing dietary intake of DHA may provide some protection against retinopathy in individuals with type 2 diabetes.
RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES
(2023)
Review
Chemistry, Analytical
Anthony J. Politza, Reza Nouri, Weihua Guan
Summary: Digital CRISPR (dCRISPR) assays are a new and emerging platform for molecular diagnostics that provide absolute quantification and increased sensitivity compared to traditional CRISPR assays. With features such as ultra-specific targeting, isothermal operation, and rapid detection, dCRISPR systems are well-positioned to lead the field of molecular diagnostics.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Aneesh Kshirsagar, Gihoon Choi, Vishaka Santosh, Tara Harvey, Robert Cory Bernhards, Weihua Guan
Summary: In order to achieve the World Health Organization's goal of eliminating malaria from developing/resource-limited economies, there is a need for a low-cost, highly sensitive, and specific screening method. We have developed a handheld nucleic acid testing device that can detect malaria infection (specifically Plasmodium falciparum) from a whole blood sample using on-chip automated sample preparation. The device utilizes a simple purification-free protocol and a piezo pump pressure-driven microfluidic cartridge for sample preparation. By monitoring the fluorescence generated from the loop mediated isothermal amplification reaction, the parasite genomic DNA concentration can be estimated. The device has achieved a sensitivity of approximately 0.42 parasites/μL of whole blood, making it suitable for detecting asymptomatic malaria parasite carriers.
Article
Nanoscience & Nanotechnology
Ming Dong, Reza Nouri, Zifan Tang, Weihua Guan
Summary: Laser-assisted controlled dielectric breakdown (LaCBD) is a promising method for nanopore fabrication due to its localization capability. However, we discovered the formation of debris around the nanopore region in LaCBD, which is not observed in conventional methods. The debris is more evident at higher laser power and voltage stress and is asymmetrically distributed on the membrane. Unexpected rectified ionic and molecular transport was also found in LaCBD nanopores with debris. Based on these findings, a model describing the debris formation kinetics in LaCBD was developed and validated.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Reza Nouri, Yuqian Jiang, Anthony J. Politza, Tianyi Liu, Wallace H. Greene, Yusheng Zhu, Jonathan J. Nunez, Xiaojun Lian, Weihua Guan
Summary: This study reported a new digital CRISPR-Cas13 assay (dCRISPR) using automated membrane-based partitioning to achieve absolute quantification of HIV-1 viral RNAs. The method does not require amplification and can rapidly quantify RNA samples over a wide range in a short period of time.
Article
Biophysics
Wenchang Zhang, Ya Li, Bing Chen, Yuan Zhang, Ziqiang Du, Feibin Xiang, Yu Hu, Xiaochen Meng, Chunliang Shang, Shengfa Liang, Xiaonan Yang, Weihua Guan
Summary: In this study, a fully integrated point-of-care testing (POCT) device for blood cell count (BCC) was developed using in-flow imaging. The device showed improved identification and counting accuracy of BCC analysis. The integration of a miniaturized magnetic stirring module ensured temporal stability of cell concentration. Multi-frame in-flow imaging and multi-angle morphology analysis were used to improve identification accuracy. The device was validated with healthy whole blood samples and clinical patients, showing good agreement with commercial clinical equipment. The device is user-friendly and cost-effective, making it a potential tool for diagnosing abnormal blood cell morphology or concentration in field settings.
BIOSENSORS & BIOELECTRONICS
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
Debora Kamin Mukaz, Aixin Li, Pamela L. Lutsey, Neil A. Zakai, Weihua Guan, Aaron R. Folsom, Mary Cushman
Meeting Abstract
Cardiac & Cardiovascular Systems
Aixin Li, Ingrid Jacobson, Brian T. Steffen, Jiaqi Xie, Nathan Pankratz, John Lane, Dan E. Arking, Thomas Mosley, Weihua Guan, Sanaz Sedaghat, Jim S. Pankow, Pamela L. Lutsey, Weihong Tang
Meeting Abstract
Cardiac & Cardiovascular Systems
Ethan Moser, Brian T. Steffen, Jiaqi Xie, Nathan Pankratz, John Lane, Dan E. Arking, Thomas Mosley, Weihua Guan, Sanaz Sedaghat, Jim S. Pankow, Pamela L. Lutsey, Weihong Tang
Meeting Abstract
Cardiac & Cardiovascular Systems
Andres V. Ardisson Korat, Frank Qian, Vasanti Malik, Marcus Lehr, Fumiaki Imamura, Nathan Tintle, Markku Laakso, Rob van Dam, William Harris, Matti Marklund, Cecilia Samieri, MacKenzie Senn, Karin Leander, Nita Forouhi, Ulf Riserus, Kou-Liong Chien, Amelia Chen, Alexis Wood, Weihua Guan, Michael Y. Tsai, Rachel Murphy, David S. Siscovick, Rozenn Lemaitre
Article
Public, Environmental & Occupational Health
Siobhan K. McMahon, Kait Macheledt, Elizabeth A. Choma, Beth A. Lewis, Weihua Guan, Jean F. Wyman, Alexander J. Rothman
Summary: This case study assessed the behavior change content of an intervention under development by integrating existing frameworks. The findings provide procedures for collecting, coding, and analyzing behavior change techniques in an intervention. Applying these procedures yielded detailed information on behavior change ingredients, which can strengthen fidelity evaluations and future intervention development. This early, integrated approach has potential benefits and raises questions about incorporating and disseminating behavior change information in research outcomes reporting.
TRANSLATIONAL BEHAVIORAL MEDICINE
(2023)
Article
Physics, Applied
Ming Dong, Zifan Tang, Weihua Guan
Summary: Evaluation of nanopore quality is crucial in developing solid-state nanopore sensors for single molecule detection. Existing studies commonly compare noise values to assess the superiority of nanopores. However, relying solely on noise comparisons can lead to inaccurate evaluations. This work emphasizes the importance of reporting baseline current and suggests using signal-to-noise ratio (SNR) as a primary evaluation tool for nanopore sensors.
APPLIED PHYSICS LETTERS
(2023)
Meeting Abstract
Oncology
Shuo Wang, Anne H. Blaes, Josef Coresh, Corinne E. Joshu, James S. Pankow, Bharat Thyagarajan, Elizabeth A. Platz, Weihua Guan, Anna Prizment