期刊
NANO LETTERS
卷 19, 期 11, 页码 8109-8117出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.9b03331
关键词
Photoacoustic imaging; microbubbles; background-free imaging; ultrasound-responsive
类别
资金
- National Natural Science Foundation of China [21927803, 51525203, 51803146, 51761145041]
- National Basic Research Programs of China [2016YFA0201200]
- Collaborative Innovation Center of Suzhou Nano Science and Technology
- 111 program from the Ministry of Education of China
- China Postdoctoral Science Foundation [7131703518]
Photoacoustic (PA) imaging based on the photon-to-ultrasound conversion allows the imaging of optical absorbers in deep tissues with high spatial resolution. However, the inherent optical absorbance of biomolecules (e.g., hemoglobin, melanin, etc.) would show up as tissue background signals to interfere with signals from the contrast agent during in vivo PA imaging, limiting the imaging sensitivity. Herein, an ultrasound (US)-responsive PA imaging probe based on microbubbles (MBs) containing gold nanoparticles (Au NPs) is designed for in vivo background-free PA imaging. The obtained Au@lip MBs with separated Au NPs decorated within the lipid shell of MBs show low PA signals under near-infrared (NIR) excitation. Interestingly, under exposure to US pulses, those Au@lip MBs would burst to form nanoscale aggregates of Au@lip NPs, which exhibit significantly enhanced NIR PA signals due to their red-shifted surface plasmon resonance. Therefore, by subtracting the PA image captured pre-US burst from that captured post-US burst, the tissue background PA signals could be deducted to enable background-free PA imaging with high sensitivities as demonstrated by multiple ex vivo and in vivo experiments. This work presents a simple yet effective strategy to deduct background signals during PA imaging, which is promising for accurate PA detection of targets in tissues with a strong background.
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