Review
Cell Biology
Chunfeng Xu, Mingjie Wang, Behrouz Zandieh-Doulabi, Wei Sun, Lingfei Wei, Yuelian Liu
Summary: BMP-2, while potentially useful in repairing bone defects after OS resection, has limited application due to concerns about its tumorigenicity. Studies are focusing on understanding the impact of BMSCs' response to BMP-2 on OS development to pave the way for safer and broader clinical use of BMP-2 in the future.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Juan C. Henao, Adriana Grismaldo, Alfonso Barreto, Viviana M. Rodriguez-Pardo, Claudia Camila Mejia-Cruz, Efrain Leal-Garcia, Rafael Perez-Nunez, Patricio Rojas, Ramon Latorre, Ingrid Carvacho, Yolima P. Torres
Summary: The study demonstrates that TRPM8 channels are expressed in human bone marrow MSCs and are involved in osteogenic differentiation, with agonists promoting osteogenic differentiation and antagonists leading to a decrease in osteogenic differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Medicinal
Zhikang Su, Ding Chen, Jiangyon Huang, Zitian Liang, Wen Ren, Zeyu Zhang, Qianzhou Jiang, Tao Luo, Lvhua Guo
Summary: This study found that isoliquiritin (ISL) can improve osteoporosis by promoting autophagy and regulating the MAPK signaling pathway. The experimental results showed that ISL has a therapeutic effect in promoting bone formation without inducing toxicity to vital organs.
PHYTOTHERAPY RESEARCH
(2023)
Article
Chemistry, Medicinal
Yanran Yang, Daiyun Chen, Yilin Li, Jinghua Zou, Ruiqi Han, Hongkun Li, Jun Zhang
Summary: Puerarin has a positive impact on the proliferation and osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells, and promotes new bone formation. In a rat RME model, puerarin solution enhances osteogenesis.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2022)
Article
Biotechnology & Applied Microbiology
Min Wang, Tianming Dai, Qingqi Meng, Wen Wang, Siming Li
Summary: This study aimed to investigate the regulatory effects of miR-28 on osteogenic differentiation of hBMMSCs. It was found that miR-28 could inhibit STAT1 expression, upregulate AKP and RUNX2 expression, and promote osteogenic differentiation.
Article
Cell Biology
Dawoon Baek, Kwang Hwan Park, Kyoung-Mi Lee, Sujin Jung, Soyeong Joung, Jihyun Kim, Jin Woo Lee
Summary: The study revealed that overexpression of USP53 can enhance osteogenic differentiation of hBMSCs by promoting the degradation of β-catenin through interaction with FBXO31, potentially promoting bone regeneration. Therefore, USP53 may be a promising target for gene therapy in bone regeneration.
CELL DEATH & DISEASE
(2021)
Article
Cell Biology
Jing Wang, Qiang Fu, Jian Yang, Jin-Long Liu, Shu-Ming Hou, Xing Huang, Jia-Shi Cao, Tie -Long Liu, Kun-Zheng Wang
Summary: This study revealed that the RNA N6-methyladenosine demethylase FTO promotes osteoporosis by demethylating runx2 mRNA and inhibiting osteogenic differentiation. The up-regulation of m6A methylated RNA and down-regulation of FTO were observed in BMSCs from OP patients, leading to compromised osteogenic potential. Inhibition of FTO effectively improved bone formation in ovariectomized osteoporotic mice.
Article
Dentistry, Oral Surgery & Medicine
Shuzuo Zhou, Gang Zhang, Kun Wang, Zhong Yang, Yinghui Tan
Summary: This study revealed the critical role of METTL3 in osteogenic differentiation of BMSCs through the m(6)A/IGF2BP1/RUNX2 signaling axis. This finding provides a potential therapeutic target for maxillofacial bone defect treatment.
Article
Biophysics
Zhibiao Bai, Kai Hu, Zeyu Shou, Han Zhou, Jiahuan Yu, Hongming Meng, Liangyan Chen, Tiantian Yu, Ruofei Lu, Na Li, Chun Chen
Summary: This study investigated the effect of PC/Mucin multilayers on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). The results showed that PC/Mucin multilayers promoted the differentiation of BMSCs into osteoblasts and this effect was mediated by the activation of the Wnt/β-catenin pathway. These findings have important implications for the development of materials and coatings for bone regeneration.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Engineering, Biomedical
Meng Li, Jing Wu, Wenbo Geng, Yulu Yang, Xuan Li, Kun Xu, Ke Li, Yan Li, Qiaojian Duan, Pengfei Gao, Kaiyong Cai
Summary: Localized corrosion of 316L stainless steel reduces the viability, adhesion, proliferation, and osteogenic differentiation abilities of BMSCs. It causes cytotoxicity through the release of metallic ions and generates reactive oxygen species (ROS) around the corrosion pits, leading to oxidative stress and cell membrane depolarization. This study provides insights into corrosion-mediated degradation of cell function and material-cell interactions.
Article
Endocrinology & Metabolism
Chengyu Zhu, Haili Ding, Liang Shi, Shihua Zhang, Xiaoyang Tong, Mei Huang, Lifei Liu, Xiaotian Guan, Jun Zou, Yu Yuan, Xi Chen
Summary: This study aimed to investigate the impact of running exercise on bone health in aging mice and explore the role of SIRT1 in controlling autophagy and osteogenic differentiation of BMSCs. The results showed that aging mice had lower bone mass, BMD, and autophagy compared to young mice, while running exercise improved BMD and bone mass and increased autophagy in bone cells. Mechanical loading enhanced osteogenic differentiation and autophagy in BMSCs, and the knockdown of SIRT1 in BMSCs demonstrated that SIRT1-mediated autophagy was involved in the activation of osteogenic differentiation by mechanical loading.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Jia Li, Juan Zhao, Yangbo Xu, Antian Xu, Fuming He
Summary: This study investigates the effect of implant surface on blood-material interactions and subsequent BMSC cellular behaviors. The SLA surface induces earlier and stronger blood clot formation, and enhances the adhesion, proliferation, and migration of BMSCs. The SLA surface also shows higher levels of osteogenic-related genes, ALP activity, and calcium nodule formation. These findings highlight the importance of SLA titanium surfaces in influencing blood clot formation and coordinating surface-blood interactions and cell biological processes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Laura Alves, Vanessa Machado, Joao Botelho, Jose Joao Mendes, Joaquim M. S. Cabral, Claudia L. da Silva, Marta S. Carvalho
Summary: Cell-based therapies using PDLSC may be a promising alternative to MSC(M) and MSC(AT) for periodontal regeneration, as PDLSC showed enhanced proliferative and osteogenic potential. PDLSC expressed osteogenic markers and had higher calcium content and upregulated osteogenic/periodontal genes compared to MSC(M) and MSC(AT). CD146 was specifically expressed by PDLSC, which also had a higher proliferative capacity. However, alkaline phosphatase activity did not increase in PDLSC.
Article
Immunology
Mingjun Li, Xiangwen Shi, Yipeng Wu, Baochuang Qi, Chaoqun Zhang, Bin Wang, Bihuan Zhang, Yongqing Xu
Summary: This study explores the mechanisms of BMSCs injury and osteogenic differentiation inhibition in osteomyelitis, and identifies a potential therapeutic target. The results show that knocking down the Pmepa1 gene can alleviate infection-induced cell injury and promote osteoblast differentiation. The findings are supported by results from a rat model.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Materials Science, Biomaterials
Bangguo Wei, Wenrui Wang, Xiangyu Liu, Chenxi Xu, Yanan Wang, Ziqi Wang, Jinnuo Xu, Jianzhong Guan, Pinghui Zhou, Yingji Mao
Summary: This study developed an innovative bone engineering scaffold based on GelMA hydrogel loaded with Res-SLNs, which demonstrated improved osteogenic differentiation of BMSCs, particularly with the optimal 0.02 Res-SLNs/GelMA scaffold. The slow and steady release of Res from the scaffold showed potential for application in bone tissue engineering.
REGENERATIVE BIOMATERIALS
(2021)