期刊
MOLECULAR BIOLOGY REPORTS
卷 47, 期 2, 页码 1099-1106出版社
SPRINGER
DOI: 10.1007/s11033-019-05206-7
关键词
Sogatella furcifera; Kruppel-homolog 1; Reproduction; Juvenile hormone; RNA interference
资金
- National Natural Science Foundation of China [31572005]
- Double First-class Construction Project of Hunan Agricultural University [SYL2019029]
Juvenile hormone (JH) plays a pivotal role in insect reproduction. The Kruppel-homolog 1 (Kr-h1) is a JH-inducible zinc finger transcription factor that has also been found to play a role in insect reproduction, however, its function varies across species. In this study, we cloned SfKr-h1 from Sogatella furcifera and investigated its role in ovarian development. The open reading frame of SfKr-h1 is 1 800 bp encoding 599 amino acids. The putative amino acid sequence of SfKr-h1 contains eight putative C2H2-type zinc finger domains and is highly homologous with the Kr-h1s of other hemipteran species. Expression of SfKr-h1 peaked 96 h after adult emergence and was highest in the ovary. RNA interference (RNAi) knockdown of SfKr-h1 substantially reduced the transcription of SfVg, and arrested ovarian development. These results suggest that SfKr-h1 is critical for normal ovarian development in S. furcifera.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据