Article
Pharmacology & Pharmacy
Li-Jian Chen, Jie-Tao He, Ming Pan, Jia-Li Liu, Kai-Kai Zhang, Jia-Hao Li, Li-Bin Wang, Ling-Ling Xu, Yu-Kui Chen, Qin-Yao Zhang, Dong-Ri Li, Jing-Tao Xu, Xiao-Li Xie
Summary: The study found that antibiotics can attenuate METH-induced hepatotoxicity by regulating oxidative stress and the TLR4/MyD88/Traf6 axis, suggesting a potential protective role of antibiotics in preventing liver injury caused by METH.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Environmental Sciences
Bingjian Cui, Chuncheng Liu, Chao Hu, Shengxian Liang
Summary: This study explores the physiological and gene expression characteristics of perennial ryegrass under cadmium (Cd) stress. High concentrations of Cd significantly inhibit the growth of ryegrass, while lower concentrations have minor effects. The activity levels of antioxidant enzymes increase in Cd-treated ryegrass leaves. The transcriptomic analysis identifies 1103 differentially expressed genes (DEGs) and reveals significant changes in molecular regulatory pathways.
Article
Biotechnology & Applied Microbiology
C. Covas, A. Vaz, B. Almeida, J. Lourenco, G. Figueiredo, O. L. Franco, S. Mendo, T. Caetano
Summary: Secondary metabolites are bioactive compounds that are challenging to produce in the laboratory. This study used RNA-seq to investigate the cellular pathways of Pedobacter lusitanus NL19 modulated by high concentrations of peptone from casein (PC). The results showed that high PC concentrations significantly repressed the biosynthesis of biotin and the production of certain nonribosomal peptides, highlighting the effect of PC on gene expression and metabolic pathways.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Genetics & Heredity
Wenwu Chen, Haiming Ma, Biao Li, Fang Yang, Yu Xiao, Yan Gong, Zhi Li, Ting Li, Qinghua Zeng, Kang Xu, Yehui Duan
Summary: This study systematically analyzed circRNAs in the liver of Ningxiang pigs and revealed their expression profiles at different developmental stages. The results showed that these circRNAs are involved in pathways related to lipid metabolism and signal transduction, and a regulatory network was constructed.
Article
Veterinary Sciences
Janne G. Lyngby, Marta Godia, Louise Brogaard, Annemarie T. Kristensen, Merete Fredholm, Ellen Skancke, Joanna Morris, Nana Dupont, Silke Salavati Schmitz, David Argyle, Armand Sanchez, Charlotte R. Bjornvad, Susanna Cirera, Lise N. Nielsen
Summary: This study found that specific microRNAs in feces and serum of dogs can be used as noninvasive biomarkers to differentiate gastrointestinal cancer from chronic inflammatory enteropathy. MiR-451 and miR-223 showed the best diagnostic performance, and combining them slightly improved the diagnostic effectiveness.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2022)
Article
Agriculture, Multidisciplinary
Suzana Tiemi Ivamoto-Suzuki, Jose Miguel Celedon, Macaire M. S. Yuen, Ci Kitzberger, Douglas Silva Domingues, Jorg Bohlmann, Luiz Filipe Protasio Pereira
Summary: The biochemical profile of coffee beans determines the quality characteristics and health benefits of the coffee beverage. Ent-kaurene, a precursor of gibberellin synthesis in plants, also serves as an intermediate in specialized diterpenoid metabolism in coffee, leading to the production of more than 1,000 different metabolites. Diterpenes in coffee, including cafestol and kahweol, have nutraceutical effects such as antioxidant, anticarcinogenic, and anti-inflammatory properties. This study identified and characterized genes involved in the production of ent-kaurene in Coffea arabica, providing insights into the biosynthesis of cafestol and kahweol.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Cell Biology
Athina Markou, E. Tzanikou, G. Kallergi, E. Pantazaka, V. Georgoulias, A. Kotsakis, E. Lianidou
Summary: The study revealed a high frequency of MCT4 overexpression in CTCs from early NSCLC patients, which was significantly associated with disease progression, suggesting its importance in the metastatic process and potential as a target for cancer therapy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Environmental Sciences
Honghong Li, Sheng Liu, Lichao Chen, Jie Luo, Dongqiang Zeng, Xuesheng Li
Summary: Thiamethoxam significantly increased JH3 levels in honey bee larvae without affecting the transcript levels of major metabolic enzymes. Exposure to thiamethoxam resulted in 140 differentially expressed genes, with upregulation of P450 CYP6AS5 and alterations in ion-related, odourant-related, and gustatory receptors. Amino acid metabolism and protein digestion and absorption were influenced by thiamethoxam exposure, potentially affecting honey bee caste differentiation and foraging behavior.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biochemistry & Molecular Biology
Wei Xi, Chenyang Hao, Tian Li, Huajun Wang, Xueyong Zhang
Summary: In this study, stress-related genes related to drought tolerance response were identified in two common wheat cultivars. The results showed that these genes are valuable resources for wheat breeding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Maria-Alexandra Papadimitriou, Konstantinos Soureas, Aristea-Maria Papanota, Panagiotis Tsiakanikas, Panagiotis G. G. Adamopoulos, Ioannis Ntanasis-Stathopoulos, Panagiotis Malandrakis, Maria Gavriatopoulou, Diamantis C. C. Sideris, Efstathios Kastritis, Margaritis Avgeris, Meletios-Athanasios Dimopoulos, Evangelos Terpos, Andreas Scorilas
Summary: The overexpression of miR-25 is associated with poor prognosis and disease progression in multiple myeloma patients, and it can be used as a powerful predictive tool for personalized treatment decisions.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Review
Oncology
Marcel Kemper, Carolin Krekeler, Kerstin Menck, Georg Lenz, Georg Evers, Arik Bernard Schulze, Annalen Bleckmann
Summary: Liquid biopsy is a novel method in cancer diagnostics that is less invasive for patients compared to conventional tissue biopsies. This review provides an overview of lung cancer biomarkers identified in liquid biopsy assays and discusses the differences, current applications, and future perspectives of liquid biopsies in lung cancer.
Article
Microbiology
Tamirat Tefera Temesgen, Kristoffer Relling Tysnes, Lucy Jane Robertson
Summary: This study presents a novel approach for evaluating the effectiveness of inactivating Cryptosporidium oocysts and can be applied to similar investigations with other pathogens.
Article
Oncology
Elena Espinosa Garcia, Macarena Arroyo Varela, Rafael Larrosa Jimenez, Josefa Gomez-Maldonado, Manuel Angel Cobo Dols, M. Gonzalo Claros, Rocio Bautista Moreno
Summary: This study expands the list of useful biomarkers for early diagnosis of lung cancer through liquid biopsies. Potential miRNA biomarkers in liquid biopsies were identified and validated in large cohorts. These candidate miRNA biomarkers can be utilized to form diagnostic predictive biomarkers for lung cancer in liquid biopsies.
CLINICAL & TRANSLATIONAL ONCOLOGY
(2023)
Article
Plant Sciences
Xin Liu, Yingbo Gao, Xinyi Zhao, Xiaoxiang Zhang, Linli Ben, Zongliang Li, Guichun Dong, Juan Zhou, Jianye Huang, Youli Yao
Summary: This study identified suitable reference genes for different rice tissues under drought and salt stress by using RNA-seq datasets. The results showed that OsMED7, OsACT1, and OsOS-9 were the robust reference genes for leaf samples, OsACT1, OsZOS3-23, and OsGDCP were recommended for shoots, and OsMED7, OsOS-9, and OsGDCP were the most reliable reference genes for roots. These findings are valuable for improving the accuracy of gene expression in rice under similar conditions through the selection of appropriate reference genes.
Article
Medicine, General & Internal
Eleonora De Martino, Ilaria Gandin, Eros Azzalini, Cesare Massone, Maria Antonietta Pizzichetta, Erika Giulioni, Sanja Javor, Caterina Pinzani, Claudio Conforti, Iris Zalaudek, Serena Bonin
Summary: This study analyzed the expression of 7 miRNAs in plasma-derived exosomes and found that 3 miRNAs were differentially expressed in melanoma patients compared to controls. The expression of these miRNAs may serve as a promising ancillary tool for melanoma biomarkers, and even for discriminating between nevi and melanoma.
FRONTIERS IN MEDICINE
(2023)
Review
Medicine, General & Internal
Sarka Benesova, Mikael Kubista, Lukas Valihrach
Summary: miRNAs are small RNA molecules with regulatory roles in physiological and pathological processes, serving as non-invasive biomarkers. Small RNA-seq methods enable comprehensive analysis of miRNAs, yet face challenges in technical bias and capturing true miRNA expression accurately in applications. New approaches have been introduced to minimize bias in small RNA-seq protocols, enhancing their potential in miRNA research.
Article
Pathology
Peter Androvic, Sarka Benesova, Eva Rohlova, Mikael Kubista, Lukas Valihrach
Summary: This study compared the performance of commercially available small RNA-Seq library preparation methods for quantification of miRNAs in biofluids. The results showed that there was no single protocol outperforming others across all metrics, and different methods exhibited biases in measuring miRNA profiles, with varying performance in minimizing biases.
JOURNAL OF MOLECULAR DIAGNOSTICS
(2022)
Article
Geriatrics & Gerontology
Jana Tureckova, Monika Kamenicka, Denisa Kolenicova, Tereza Filipi, Zuzana Hermanova, Jan Kriska, Lenka Meszarosova, Barbora Pukajova, Lukas Valihrach, Peter Androvic, Daniel Zucha, Martina Chmelova, Lydia Vargova, Miroslava Anderova
Summary: This study reveals the impact of amyloid-beta toxicity and tau pathology on astrocyte swelling, volume recovery, and extracellular space diffusion in a mouse model of Alzheimer's disease. AD progression affects astrocyte ionic/neurotransmitter uptake, leading to enlarged extracellular space and increased obstacles. These changes may disrupt intercellular communication and contribute to cytotoxicity.
FRONTIERS IN AGING NEUROSCIENCE
(2022)
Article
Biology
Yoichiro Shibuya, Akishige Hokugo, Hiroko Okawa, Takeru Kondo, Daniel Khalil, Lixin Wang, Yvonne Roca, Adam Clements, Hodaka Sasaki, Ella Berry, Ichiro Nishimura, Reza Jarrahy
Summary: Attempts to minimize scarring after surgery remain challenging for surgeons. This study identified a compound (Dwn1) that suppressed the expression of Npas2, a protein involved in wound healing. Treatment with Dwn1 resulted in faster and stronger healing of dermal wounds in mice, with reduced scarring.
Article
Neurosciences
Rocio Valle-Bautista, Berenice Marquez-Valadez, Gabriel Herrera-Lopez, Ernesto Griego, Emilio J. Galvan, Nestor-Fabian Diaz, Jose-Antonio Arias-Montano, Anayansi Molina-Hernandez
Summary: The transient histaminergic system, activated through H-1 receptor, plays a role in the differentiation of deep-layer cortical neurons. Blocking H-1 receptors with chlorpheniramine during neurogenesis leads to decreased levels of deep-layer cortical neurons and impairs learning, wakefulness, and nociception. This study evaluated the long-lasting effects of chlorpheniramine on the differentiation of deep-layer cortical neurons in neonates and 21-day-old pups. The results showed altered expression and distribution of cortical markers, as well as impaired dendritic arborization and lower excitability in response to histamine. These findings suggest the importance of H1 receptors in cortical neurogenesis and its impact on motor activity and cognition.
FRONTIERS IN NEUROSCIENCE
(2022)
Review
Cell Biology
Daniel Zucha, Mikael Kubista, Lukas Valihrach
Summary: Reverse transcription quantitative PCR (RT-qPCR) is a valuable tool for understanding gene expression, particularly in single-cell analysis. This article discusses the limitations of single-cell collection methods, emphasizes the importance of reverse transcription, provides recommendations for preamplification and primer design, and summarizes essential data processing steps. The detailed protocol in the appendix allows any researcher to conduct high-standard scRT-qPCR measurements.
Article
Neurosciences
Nilton Akio Muto, Moises Hamoy, Chryslen Brenda da Silva Ferreira, Akira Otake Hamoy, David Cristian Rodrigues Lucas, Vanessa Joia de Mello, Herve Rogez
Summary: This study demonstrated the anticonvulsant protective properties of Euterpe oleracea stone extract (EEOS) through EEG profiling in induced convulsing rats. The mechanism of action of EEOS is similar to diazepam (DZP) and involves the benzodiazepine (BDZ) subunit of the GABAA receptor.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Microbiology
Maria Gisele Goncalves, Lucila Okuyama Fukasawa, Karoline Rodrigues Campos, Fabio Takenori Higa, Adele Caterino-de-Araujo
Summary: Brazil currently has the highest number of individuals infected with human T-lymphotropic virus 1- and 2- (HTLV-1 and HTLV-2) globally. Molecular protocols and commercial assays for HTLV-1/-2 diagnosis are not available or validated by the Brazilian regulatory agency. In this study, two in-house multiplex quantitative real-time PCR assays were developed and validated for HTLV-1/-2 diagnosis. The assays showed high sensitivity and specificity and could resolve issues with indeterminate and untyped results in HTLV western blotting.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Benjamin Hackl, Peter Lukacs, Janine Ebner, Krisztina Pesti, Nicholas Haechl, Matyas C. Foeldi, Elena Lilliu, Klaus Schicker, Helmut Kubista, Anna Stary-Weinzinger, Karlheinz Hilber, Arpad Mike, Hannes Todt, Xaver Koenig
Summary: Ivabradine acts as an atypical inhibitor of voltage-gated sodium channels, affecting heart rate and cardiac conduction system, which may provide potential basis for anti-arrhythmic effects.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Agnieszka Smieszek, Klaudia Marcinkowska, Ariadna Pielok, Mateusz Sikora, Lukas Valihrach, Elaine Carnevale, Krzysztof Marycz
Summary: This study investigated the effects of obesity on the cellular features of equine endometrial progenitor cells. It found that obesity leads to a decline in cell proliferation, metabolic activity, and an increase in oxidative stress. Obesity also alters the expression of functional markers and impairs the regenerative capacity of endometrial progenitor cells.
Article
Orthopedics
Bernhard Springer, Robin Dreisbach, Klaus-Dieter Schatz, Bernd Kubista, Wenzel Waldstein
Summary: This study evaluated the information quality of YouTube videos regarding postoperative patient information and rehabilitation after arthroscopic rotator cuff repair. Most of the videos were of poor quality, with videos uploaded by medically trained professionals offering better information. The findings suggest that physicians should provide alternative sources of high-quality information to patients.
ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY
(2023)
Article
Multidisciplinary Sciences
Romana Bohuslavova, Valeria Fabriciova, Ondrej Smolik, Laura Lebron-Mora, Pavel Abaffy, Sarka Benesova, Daniel Zucha, Lukas Valihrach, Zuzana Berkova, Frantisek Saudek, Gabriela Pavlinkova
Summary: NEUROD1 is a transcription factor that plays an important role in pancreatic development and is crucial for the differentiation of endocrine cells. Its deficiency disrupts endocrine cell identity acquisition and compromises their differentiation and functional properties.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Kieran D. O'Malley, Henry Schofield, Patrick G. R. Wright, Daniel Hargreaves, Tom Kitching, Marina Bollo Palacios, Fiona Mathews
Summary: Identifying peaks of bat activity through acoustic monitoring can help locate bat colonies, and a triage system based on this method can be used to prioritize survey efforts. This study successfully developed a methodology for predicting woodland occupancy by barbastelle bats using acoustic data, and validated it through citizen science surveys and specialist tracking surveys.
Article
Agriculture, Dairy & Animal Science
Jianfei Chen, Ziwei Chen, Jilong Zhang, Wuchao Zhuang, Xiaotong Zheng
Summary: This study utilized different algorithms to evaluate the stability of internal reference genes in the gastrointestinal tract of chickens. The most suitable reference genes for each type of GI tissue were identified. Commonly used internal reference genes exhibited poor stability and are not recommended for future gene expression studies in the GI region.
Article
Mycology
Relber Aguiar Goncales, Igor Godinho Portis, Thaila Fernanda dos Reis, Luiz Roberto Basso Junior, Roberto Martinez, Heng Zhu, Maristela Pereira, Celia Maria de Almeida Soares, Paulo Sergio Rodrigues Coelho
Summary: In this study, expression analysis of GPI-anchored proteins in Paracoccidioides brasiliensis was conducted. The findings revealed increased expression levels of certain proteins in the mycelium and yeast forms, and these proteins showed immune reactivity against sera obtained from PCM patients. These results provide valuable information about the function of GPI-anchored proteins and their potential as diagnostic markers.
FRONTIERS IN FUNGAL BIOLOGY
(2023)
