期刊
MICROBIAL PATHOGENESIS
卷 136, 期 -, 页码 -出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2019.103706
关键词
Riemerella anatipestifer; Cas9; Virulence; Immunity; RNAseq
资金
- Natural Science Foundation of China [31872498]
- Natural Science Foundation of Hubei Province [2015CFB268]
- Wuhan Science and Technology Bureau [2015020101010070]
Riemerellosis, a Riemerella anatipestifer infection, can cause meningitis, pericarditis, parahepatitis, and airsacculitis in ducks, leading to serious economic losses in the duck meat industry. However, the molecular mechanism of the pathogenesis and virulence factors of this infection are poorly understood. In the present study, we created a mutant strain RA-YM Delta Cas9 using trans-conjugation. Bacterial virulence tests indicated that the median lethal dose (LD50) of RA-YM Delta Cas9 was 5.01 x 10(7) CFU, significantly lower than that of the RA-YM strain, which was 1.58 x 10(3) CFU. The distribution and blood bacterial load from the infection groups showed no significant difference in the brain between the RA-YM Delta Cas9 mutant and the wild-type RA-YM strains, however, the number of mutant strains were significantly reduced in the liver, heart, and blood. Animal immunization experiments demonstrated that the intranasal administration of RA-YM Delta Cas9 in ducklings provided 80% protection after challenge with the wild-type strain, showing potential use as a live mucosal vaccine. RNAseq analysis indicated that Cas9 protein played a regulatory role in gene expression. This study is the first to report on the involvement of Cas9 in the regulation and pathogenesis of R. anatipestifer, and provides a theoretical basis for the development of relevant genetic engineering vaccines.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据