Review
Biotechnology & Applied Microbiology
Govinda R. Navale, Mahesh S. Dharne, Sandip S. Shinde
Summary: Isoprenoids, also known as terpenoids, are diverse organic compounds found abundantly in nature, with various applications in pharmaceutical, nutraceutical, and chemical industries. Recent advancements in metabolic engineering have enabled the production of isoprenoids in heterologous host systems like Escherichia coli and Saccharomyces cerevisiae. Challenges and strategies for scale-up and engineering of isoprenoids in heterologous host systems have been discussed in detail.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ziling Ye, Yanglei Huang, Bin Shi, Zilei Xiang, Zhu Tian, Man Huang, Liangliang Wu, Zixin Deng, Kun Shen, Tiangang Liu
Summary: In this study, we achieved high production of (+)-valencene by engineering Saccharomyces cerevisiae, and successfully chemically synthesized (+)-Nootkatone from it. This research achievement is of great importance for addressing the insufficient market demand and stabilizing the supply of (+)-Nootkatone.
METABOLIC ENGINEERING
(2022)
Article
Agriculture, Multidisciplinary
Xiao Kong, Yaokang Wu, Wenwen Yu, Yanfeng Liu, Jianghua Li, Guocheng Du, Xueqin Lv, Long Liu
Summary: In this study, efficient biosynthesis of limonene in Saccharomyces cerevisiae was achieved through metabolic engineering strategies. The metabolic flow was directed towards limonene synthesis, and the titer reached 2.63 g/L, the highest reported in S. cerevisiae.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Lijuan Zhang, Xue Liu, Jiawei Li, Yonghui Meng, Guang-Rong Zhao
Summary: This study successfully constructed a Saccharomyces cerevisiae strain for betanin production, achieving high efficiency through optimizing metabolic pathways, screening and modifying enzymes, among other methods. This work is important for the development of synthetic yeast cell factories for the production of valuable betanin and other betacyanins.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Eline D. Postma, Else-Jasmijn Hassing, Venda Mangkusaputra, Jordi Geelhoed, Pilar de la Torre, Marcel van den Broek, Christiaan Mooiman, Martin Pabst, Jean-Marc Daran, Pascale Daran-Lapujade
Summary: This study demonstrates the feasibility of extensive genome reprogramming using modular, specialized de novo-assembled neochromosomes in yeast, leading to the first de novo production of anthocyanins in a microbial cell factory. The neochromosomes mimic native chromosomes in terms of mitotic and genetic stability, serving as exclusive expression platforms for heterologous and essential metabolic routes.
METABOLIC ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Panpan Wei, Chuanbo Zhang, Xueke Bian, Wenyu Lu
Summary: In this study, an efficient biosynthetic pathway was constructed to produce carnosic acid (CA) in Saccharomyces cerevisiae. The CA titer was increased through metabolic engineering and synthetic biology strategies, providing a theoretical basis for microbial synthesis of other diterpenoids.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Zhan-Tao Zhu, Meng-Meng Du, Bei Gao, Xin-Yi Tao, Ming Zhao, Yu-Hong Ren, Feng-Qing Wang, Dong-Zhi Wei
Summary: Harnessing mitochondria for terpene biosynthesis is promising but can lead to metabolic burden and poor cell growth. Analysis shows toxicity from MVA pathway phosphorylated intermediates within mitochondria inhibits cellular functions. Combining cytoplasmic and mitochondrial engineering strategies can alleviate metabolic burden and enhance terpene production.
METABOLIC ENGINEERING
(2021)
Article
Endocrinology & Metabolism
Tao Yu, Quanli Liu, Xiang Wang, Xiangjian Liu, Yun Chen, Jens Nielsen
Summary: This study demonstrates the establishment of a synthetic decarboxylation cycle in the yeast cytosol, which enhances the production of reduced chemicals. By implementing rational design, metabolic rewiring, and recombinant expression, the researchers achieved a significant increase in free fatty acid yield.
Article
Agriculture, Multidisciplinary
Chunyang Cao, Xuan Cao, Wei Yu, Yingxi Chen, Xinping Lin, Beiwei Zhu, Yongjin J. Zhou
Summary: This study reports the rewiring of cellular metabolism in yeast to achieve high-level synthesis of (+)-valencene. The results show that metabolic transformation and multi-copy expression of sesquiterpenoid synthase can enhance the metabolic flux and precursor supply for product synthesis, leading to significantly increased production of (+)-valencene.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Review
Biotechnology & Applied Microbiology
Shiyun Li, Qiyue Zhang, Jing Wang, Yingli Liu, Yunying Zhao, Yu Deng
Summary: In order to reduce reliance on petroleum, utilizing industrial microorganisms for the biosynthesis of important chemicals from simple substrates has become a focus of interest. This review explores strategies to increase intracellular malonyl-CoA levels and advances in metabolic engineering for directing malonyl-CoA to desired derivatives, including strengthening supply, reducing consumption, and controlling levels effectively. These strategies offer new insights for enhancing malonyl-CoA derivative synthesis in microorganisms.
JOURNAL OF BIOTECHNOLOGY
(2021)
Review
Agriculture, Multidisciplinary
Wenjuan Li, Liuwei Cui, Jie Mai, Tian-Qiong Shi, Lu Lin, Zhi-Gang Zhang, Rodrigo Ledesma-Amaro, Weiliang Dong, Xiao-Jun Ji
Summary: This review summarizes the important roles and engineering strategies of Saccharomyces cerevisiae and Yarrowia lipolytica in terpene production, discusses the future prospects and challenges, and emphasizes guidelines for future studies.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Review
Biotechnology & Applied Microbiology
Mingkai Li, Mengyu Ma, Zhenke Wu, Xiqin Liang, Qiusheng Zheng, Defang Li, Tianyue An, Guoli Wang
Summary: Rare ginsenosides, which are deglycosylated derivatives of major ginsenosides, have better absorption and biological activity. Traditional preparation methods hindered their potential applications. Recent advances in understanding ginsenoside biosynthetic pathways and the use of synthetic biology techniques have allowed for large-scale production of rare ginsenosides. This review summarizes the identification of key enzymes involved in their biosynthesis and the construction of microbial chassis for their production, highlighting the importance of discovering more enzymes and improving their catalytic efficiencies for metabolic engineering of rare ginsenosides.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Yonghui Meng, Xue Liu, Lijuan Zhang, Guang-Rong Zhao
Summary: This study successfully constructed Saccharomyces cerevisiae to produce genistein from sugar using a modular engineering strategy. The researchers optimized various modules to enhance the production and conversion of intermediates, resulting in the de novo biosynthesis of genistein. This work shows promising potential for producing flavonoids and isoflavonoids through modular metabolic engineering.
Review
Biotechnology & Applied Microbiology
Ziqian Yu, Zhao Chang, Yinhua Lu, Han Xiao
Summary: Metabolic engineering of Saccharomyces cerevisiae for efficient glycerol utilization is vital for the manufacturing industry. Glycerol, a widely available alternative feedstock, is considered promising for microbial fermentation. This review introduces the metabolic pathway and regulation of glycerol in S. cerevisiae, and summarizes various strategies for improving glycerol utilization, including metabolic engineering, adaptive evolution, and reverse metabolic engineering. Proposed methods for further enhancing glycerol utilization provide insights for designing engineered S. cerevisiae.
FEMS YEAST RESEARCH
(2023)
Article
Biochemical Research Methods
Huayi Liu, Xinxin Wu, He Ma, Jian Li, Zhenyu Liu, Xufan Guo, Jia Dong, Shaolan Zou, Yunzi Luo
Summary: Efficient production of hydroxytyrosol in engineered Saccharomyces cerevisiae was achieved through a series of modifications, resulting in the highest reported titer for de novo biosynthesis of hydroxytyrosol in microorganisms.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Muzi Zhu, Ling Zhang, Fang Yang, Yaping Cha, Shuang Li, Min Zhuo, Shaobin Huang, Jianjun Li
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2020)
Article
Agriculture, Multidisciplinary
Yaping Cha, Wen Li, Tao Wu, Xia You, Hefeng Chen, Chaoyi Zhu, Min Zhuo, Bo Chen, Shuang Li
Summary: In this study, an effective screening system based on cell wall destruction was developed to explore the optimal ratio of HPO/AtCPR, leading to successful (+)-nootkatone production in Saccharomyces cerevisiae. By constructing an engineered strain and employing metabolic engineering strategies, high yields of nootkatone were achieved.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Lingling Ma, Tao Wu, Peiling Liu, Dongying Chen, Shengliang Cai, Hefeng Chen, Jingtao Zhou, Chaoyi Zhu, Shuang Li
Summary: This study developed a fermentation process using seaweed hydrolysate as a carbon source to produce nootkatone. The high yield and purity of nootkatone were achieved. Mosquito-repellent testing showed that nootkatone has a comparable effect to DEET, making it a potential eco-friendly mosquito repellent.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Physical
Xianfeng Huang, Bo Zhang, Cong Guo, Guangxing Yang, Qiao Zhang, Shuang Li, Hao Yu, Feng Peng
Summary: Noble-metal high-entropy-alloy nanoparticles (HEA NPs) synthesized via the co-reduction of noble-metal precursors in triethylene glycol demonstrate activity in the electrocatalytic oxidation of 5-hydroxymethylfurfural (HMF). The composition of the HEA NPs significantly affects the product selectivity by regulating the oxidation pathways.
SUSTAINABLE ENERGY & FUELS
(2023)
Article
Chemistry, Multidisciplinary
Chaoyi Zhu, Xia You, Tao Wu, Wen Li, Hefeng Chen, Yaping Cha, Min Zhuo, Bo Chen, Shuang Li
Summary: This study describes the efficient biotransformation of mannitol into the high value-added sesquiterpenoid valencene using an evolved strain of Saccharomyces cerevisiae. The evolved strain exhibited superior performance in mannitol assimilation and showed significantly higher valencene titer and carbon conversion efficiency compared to glucose. Further engineering strategies increased the valencene titer, and the maximum titer obtained in a bioreactor was 5.61 g L-1. This research presents a novel strategy for terpenoid production in S. cerevisiae and expands the application of renewable biomass marine macroalgae.
Article
Chemistry, Multidisciplinary
Pengfei Yuan, Junqing Dong, Weibin Zhao, Min Zhuo, Shuang Li, Shaobin Huang, Jianjun Li
Article
Chemistry, Multidisciplinary
Xiaodan Ouyang, Yapinq Cha, Wen Li, Chaoyi Zhu, Muzi Zhu, Shuang Li, Min Zhuo, Shaobin Huang, Jianjun Li
Article
Agriculture, Multidisciplinary
Tianwang Xu, Xiongliang Huang, Zhe Li, Carol Sze Ki Lin, Shuang Li
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2018)