期刊
MICROBIAL CELL FACTORIES
卷 18, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12934-019-1259-x
关键词
Yarrowia lipolytica; Metabolic engineering; Synthetic biology; Genetic tools; Genome editing; CRISPR-Cas9; Transposon; Functional genomics
资金
- NSF [CBET-1403099, CBET-1706134]
- National Institute of General Medical Sciences [NIH-R35GM133803]
- Clemson University
- NASA's Space Technology Research Grants Program [NNX15AU46G]
- NASA [NNX15AU46G, 796761] Funding Source: Federal RePORTER
Yarrowia lipolytica has emerged as a biomanufacturing platform for a variety of industrial applications. It has been demonstrated to be a robust cell factory for the production of renewable chemicals and enzymes for fuel, feed, oleochemical, nutraceutical and pharmaceutical applications. Metabolic engineering of this non-conventional yeast started through conventional molecular genetic engineering tools; however, recent advances in gene/genome editing systems, such as CRISPR-Cas9, transposons, and TALENs, has greatly expanded the applications of synthetic biology, metabolic engineering and functional genomics of Y. lipolytica. In this review we summarize the work to develop these tools and their demonstrated uses in engineering Y. lipolytica, discuss important subtleties and challenges to using these tools, and give our perspective on important gaps in gene/genome editing tools in Y. lipolytica.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据