Article
Biochemistry & Molecular Biology
Marta Toth, Szabolcs Muzsai, Krzysztof Regulski, Timea Szendi-Szatmari, Zsolt Czimmerer, Eva Rajnavolgyi, Marie-Pierre Chapot-Chartier, Attila Bacsi
Summary: The human gut symbiont Lacticaseibacillus (L.) casei has a wide range of immunomodulatory effects on the human host. This study demonstrates that human monocyte-derived dendritic cells (moDCs) can engulf L. casei BL23, and the intact bacterial cell wall and morphology play a key role in this process. The absence of the bacterial cell-wall-degrading enzyme Lc-p75 leads to defective internalization and impaired cytokine secretion by moDCs, resulting in reduced T-cell activation capacity. Formation of short bacterial chains helps to exert the potent immunomodulatory properties of L. casei BL23.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Nelio A. J. Oliveira, Handan Sevim
Summary: Dendritic cells are important cells in the immune system, and researchers are looking for efficient methods to obtain dendritic cells from human induced pluripotent stem cells. Despite some challenges, progress has been made and there is potential for clinical applications.
STEM CELLS AND DEVELOPMENT
(2022)
Review
Biochemistry & Molecular Biology
Alice Coillard, Elodie Segura
Summary: This study reviews the research on mouse mo-DC, evaluates the in vivo evidence for monocyte-derived antigen-presenting cells, and proposes that mo-DC may present antigens to effector T cells locally in tissues, playing a complementary role to cDCs.
MOLECULAR IMMUNOLOGY
(2021)
Article
Immunology
Wanjing Cen, Felix Umrath, Antonio Jose Salgado, Siegmar Reinert, Dorothea Alexander
Summary: The secretomes derived from osteogenically induced jaw periosteum-derived mesenchymal stem cells (JPCs) have inhibitory effects on the maturation of monocyte-derived dendritic cells (MoDCs), as they reduce the formation of MoDC clusters and down-regulate the expression of CD80, CD86, and CD197 on MoDCs. The secretomes also enhance the uptake capacity of MoDCs and promote the expansion of regulatory T cells. Furthermore, the secretomes affect the gene expression of MoDCs, up-regulating CD209, HLA-DRA, CSF3, IL10, and IL8, and down-regulating IFN gamma and IL12B.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Dermatology
Lydia Bellmann, Claudia Zelle-Rieser, Paul Milne, Anastasia Resteu, Christoph H. Tripp, Natascha Hermann-Kleiter, Viktoria Zaderer, Doris Wilflingseder, Paul Hoertnagl, Maria Theochari, Jessica Schulze, Mareike Rentzsch, Barbara Del Frari, Matthew Collin, Christoph Rademacher, Nikolaus Romani, Patrizia Stoitzner
Summary: Langerhans cells in the skin, critical for immune response and skin homeostasis, can be generated from monocytes with specific cytokines and ligands. These monocyte-derived LCs show potential for vaccine testing and can be an effective tool for future research.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Article
Rheumatology
Hiroki Yamada, Takanori Sasaki, Kotaro Matsumoto, Katsuya Suzuki, Masaru Takeshita, Shuhei Tanemura, Noriyasu Seki, Hideto Tsujimoto, Tsutomu Takeuchi
Summary: The study found that HLA-DR+ Tph cells were correlated with DAS28-ESR, while HLA-DR- Tph cells accurately reflected disease activity during treatment. MTX treatment independently reduced the proportion of HLA-DR+ Tph cells.
Article
Cell Biology
Thijs L. J. van Osch, Juulke Steuten, Jan Nouta, Carolien A. M. Koeleman, Arthur E. H. Bentlage, Sebastiaan Heidt, Arend Mulder, Jan Voorberg, S. Marieke van Ham, Manfred Wuhrer, Anja Ten Brinke, Gestur Vidarsson
Summary: Immune-mediated platelet refractoriness (PR) is a significant issue in platelet transfusion, mainly caused by alloantibodies against human leukocyte antigens (HLA). Variations in HLA-specific IgG responses have been observed between alloimmunized patients. This study found that different Fc glycosylation patterns did not affect phagocytosis of opsonized platelets by monocyte-derived human macrophages.
Article
Hematology
Sierra H. Root, Hector L. Aguila
Summary: Osteoclast progenitors capable of generating mature osteoclast cells have been identified in mice and humans, with the ability to differentiate into macrophages and dendritic cells as well. CD11b and CD14 expression can be used to identify cells with monocyte and osteoclast progenitor activity. Within the CD15(-) CD115(+) population, cells with the highest monocytic progenitor and osteoclastic potential were identified.
Article
Biochemistry & Molecular Biology
Maria Teresa Pagano, Katia Fecchi, Marina Pierdominici, Elena Ortona, Daniela Peruzzu
Summary: Silibinin exhibits immunosuppressive activity by inhibiting the maturation and activation of human dendritic cells, suggesting its potential application as an adjuvant therapy for autoimmune diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sei Muraoka, Kaichi Kaneko, Kaori Motomura, Junko Nishio, Toshihiro Nanki
Summary: Inflammatory conditions promote osteoclast differentiation with the help of CX3CL1, which plays a crucial role not only in accumulating inflammatory cells but also in regulating osteoclastogenesis in rheumatoid arthritis.
Article
Cell Biology
Dylan Kwart, Jing He, Subhashini Srivatsan, Clarissa Lett, Jacquelynn Golubov, Erin M. Oswald, Patrick Poon, Xuan Ye, Janelle Waite, Arielle Glatman Zaretsky, Sokol Haxhinasto, Elsa Au-Yeung, Namita T. Gupta, Joyce Chiu, Christina Adler, Samvitha Cherravuru, Evangelia Malahias, Nicole Negron, Kathryn Lanza, Angel Coppola, Min Ni, Hang Song, Yi Wei, Gurinder S. Atwal, Lynn Macdonald, Nicole Stokes Oristian, William Poueymirou, Vladimir Jankovic, Matthew Fury, Israel Lowy, Andrew J. Murphy, Matthew A. Sleeman, Bei Wang, Dimitris Skokos
Summary: This study identifies endogenous cancer cell-derived type I interferons (IFNs) as regulators of monocyte functional polarization, which in turn influences the efficacy of immunotherapies. By analyzing single-cell transcriptomic data from human and mouse tumors, the researchers distinguish and separate immunostimulatory from immunosuppressive tumor monocytes based on surface marker expression. They show that cancer cell-derived IFNs regulated by cGAS-STING pathway can polarize immunostimulatory monocytes and demonstrate that immunosuppressive monocytes can convert into immunostimulatory monocytes upon cancer cell-intrinsic cGAS-STING activation. Moreover, the study finds that human cancer cells can produce type I IFNs that polarize monocytes, and the immunostimulatory monocyte gene signature is enriched in patient tumors that respond to anti-PD-1 immunotherapy.
Article
Immunology
Sylvain Raimbault, Guillaume Monneret, Morgane Gossez, Fabienne Venet, Alexandre Belot, Franck Zekre, Solene Remy, Etienne Javouhey
Summary: In this study, the researchers examined the use of monocyte human leucocyte antigen (mHLA)-DR as a potential diagnostic marker for secondary HLH (sHLH). They found that levels of mHLA-DR were significantly higher in patients with sHLH compared to healthy children and those with septic shock. Additionally, mHLA-DR levels decreased after treatment initiation. The findings suggest that elevated mHLA-DR could aid in the diagnosis of sHLH and distinguish it from septic shock.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Frederic Coutant, Jean-Jacques Pin, Pierre Miossec
Summary: Inflammatory monocyte-derived dendritic cells exhibit a distinct phenotype with C-type lectin receptors and intracellular proteins. The interaction between infiltrating monocytes and local mesenchymal cells is crucial for the generation of Mo-DCs in rheumatoid arthritis patients.
Article
Immunology
Yongqiang Liao, Ni Xiao, Xiaoming Wang, Senhua Dai, Guiliang Wang
Summary: In this study, we found that the expression of Thymosin beta 4 x (Tmsb4x) is decreased in patients with septicemia, leading to increased inflammatory response and reduced proportion of dendritic cells (DCs). Restoration of Tmsb4x expression facilitated the differentiation of mononuclear cells into DCs.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Dermatology
Shahrzad Alimohammadi, Zsofia Penzes, Dorottya Horvath, Agnes Gyetvai, Attila Bacsi, Nikoletta Greta Kis, Akos Nemeth, Jozsef Arany, Attila Olah, Erika Lisztes, Balazs Istvan Toth, Tamas Biro, Attila Gabor Szollosi
Summary: This study compared the transcriptome of monocyte-derived Langerhans cells (moLCs) with that of immature dendritic cells using RNA sequencing. The results showed that moLCs express genes characteristic of LC2 cells and TRPV4. Activation of TRPV4 increased the expression of Langerin and intracellular calcium concentration in moLCs. Furthermore, TRPV4 activation mitigated the inflammatory responses of moLCs by decreasing cytokine production and T-cell activating capability.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
