期刊
MECHANISMS OF AGEING AND DEVELOPMENT
卷 183, 期 -, 页码 -出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mad.2019.111151
关键词
N-glycans; Placental glycoproteins; Placental glycans; Lectin microarray; MALDI-TOF MS
资金
- Ministry of Education, Science and Technological Development of the Republic of Serbia [337-00-107/2019-09/12, SK-SRB-18-0028, 173042]
- Slovak Grant Agency for Science VEGA [VEGA 2/0137/18, 2/0130/18]
- Slovak Research and Development Agency [APVV-14-0753]
Structural changes of glycans are observed in different (patho)physiological conditions. Human placental membrane (glyco)proteins were isolated from the first and third trimester placentas of mothers at different ages. By using lectin microarray, we demonstrated that the placental membrane N-glycome contains several N-glycan groups: high mannose, asialylated and sialylated biantennary moieties, bisected, core fucosylated, fucosylated at other positions (bearing terminal and/or antennary Fuc), alpha 2-6 and alpha 2-3 sialylated structures. Employing MALDI-TOF MS enabled identification of over sixty different N-glycan structures in all samples, with 17 moieties exceeding the relative abundance of 2%. The major MS peaks originated from: 1) biantennary complex type N-glycan with a bisecting GlcNAc residue and 2) a core Fuc paucimannosidic and high mannose type structures M3-M9. Age of mothers and the stage of placental development affected N-glycome. The work presented in this article is the first comprehensive mass spectrometric study of the N-glycome of human placental membrane proteins. Our results may be seen as the baseline which can serve for future MALDI MS profiling of the placental membrane N-glycome in different pathophysiological conditions.
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