Article
Thermodynamics
Shuai Yuan, Mingyong Zhou, Xijiang Liu, Qiang Li, Bingyan Jiang
Summary: Microfluidic-based enrichment techniques have been extensively studied to enhance the sensitivity of detection by increasing the concentration of analytes. In this study, a numerical model is developed to investigate the complex analyte focusing process in a microfluidic electrophoresis chip. The results show that factors such as electrode location and the number of mass transfer channels are crucial for improving the enrichment performance. After considering the Joule heating effect, the final microchip achieves significant concentration enrichment. Moreover, the method is successfully applied to the experimental enrichment of Rhodamine B and cadmium (II) ions.
INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER
(2022)
Article
Biochemical Research Methods
Gyula Kajner, Albert Keri, Adam Belteki, Sandor Valkai, Andras Der, Zsolt Geretovszky, Gabor Galbacs
Summary: This study explored the potential of online sample pretreatment steps on microfluidic chips for single particle ICP-MS measurements. By optimizing the design and functions of the chips, efficient analysis of nanoparticles with high efficiency and precision was achieved.
Review
Biotechnology & Applied Microbiology
Kaimin Wu, Xuliang He, Jinglei Wang, Ting Pan, Ran He, Feizhi Kong, Zhenmin Cao, Feiye Ju, Zhao Huang, Libo Nie
Summary: Microfluidic chip technology is a technology platform that integrates basic operation units such as processing, separation, reaction, and detection into a microchannel chip to achieve low consumption, fast and efficient sample analysis. It has the characteristics of small sample and reagent volume, fast analysis, low cost, automation, portability, high throughput, and good compatibility with other techniques. The future development of microfluidic chips lies in point-of-care testing and high throughput equipment, but there are still challenges in the design and integration of microfluidic chips, as well as the analysis of actual samples.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Jonathan Cottet, Josephine O. Oshodi, Jesse Yebouet, Andrea Leang, Ariel L. Furst, Cullen R. Buie
Summary: Surface charge is a critical feature of microbes that affects their interactions with other cells and their environment. This study presents a cost-effective method for characterizing surface charge using commercial microfluidic chips and open-source workflows, eliminating the need for expensive equipment and specialized facilities. The method enables facile electrokinetic characterization of particles and cells and improves measurement reproducibility.
Review
Biotechnology & Applied Microbiology
Sasan Jalili-Firoozinezhad, Claudia C. Miranda, Joaquim M. S. Cabral
Summary: Organ-on-chip culture technology is a promising tool for studying tissue development and homeostasis, bringing us closer to conducting human experimentation in vitro. By linking on-chip models of multiple organs, a functional human body-on-chip platform can be created, with advantages in disease modeling, therapeutic development, and personalized medicine. Current limitations of the body-on-chip approach are discussed, along with new ideas to address pending issues.
TRENDS IN BIOTECHNOLOGY
(2021)
Article
Spectroscopy
Abdallah M. Zeid, Jenny Jeehan M. Nasr, Fathalla Belal, Mohamed I. Walash, Yoshinobu Baba, Noritada Kaji
Summary: A lab-on-a-chip electrophoretic method coupled with LED-induced fluorescence detection was developed for the assay of three antiepileptic drugs in pharmaceutical formulations. The method involves derivatizing analytes with FITC offline, optimizing separation conditions, and using specific compounds to enhance separation efficiency. The sensitivity of the method was greatly enhanced through stacking, allowing for successful validation in pharmaceutical formulations.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Biochemical Research Methods
Jennifer Grant, Alican Ozkan, Crystal Oh, Gautam Mahajan, Rachelle Prantil-Baun, Donald E. Ingber
Summary: A combined simulation and experimental approach is described to predict the spatial and temporal concentration profile of a drug in a PDMS Organ Chip, allowing for drug testing without prior knowledge of partition coefficient or diffusion rate in PDMS.
Article
Biochemical Research Methods
Idris Salmon, Sergei Grebenyuk, Abdel Rahman Abdel Fattah, Gregorius Rustandi, Thomas Pilkington, Catherine Verfaillie, Adrian Ranga
Summary: The generation of tissue and organs requires close interaction with vasculature. This study presents a 3D printing-based platform that allows pluripotent stem cells to interact with vascular cells in a spatially determined manner, resulting in the formation of organized vascular networks. This method provides a means to vascularize organoids and opens new avenues for understanding and manipulating the co-development of tissue-specific organoids with vasculature.
Review
Chemistry, Analytical
Soojung Kim, Hyerin Song, Heesang Ahn, Taeyeon Kim, Jihyun Jung, Soo Kyung Cho, Dong-Myeong Shin, Jong-ryul Choi, Yoon-Hwae Hwang, Kyujung Kim
Summary: Electrical impedance biosensors combined with microfluidic devices offer high-throughput analysis of biological processes at the single-cell scale, enabling sensitive determination of drug effectiveness and toxicity. The efficiency and performance of the sensors are ultimately determined by the methods of single-cell trapping, while identifying the latest trends opens up opportunities for technological advancement. This leading technology in cell biology, pathology, and pharmacology allows for further understanding of complex cellular functions and mechanisms through precise analysis capabilities.
Review
Chemistry, Analytical
Jianyu Shi, Yating Pan, Xudong Liu, Wenjian Cao, Ying Mu, Qiangyuan Zhu
Summary: Spatial profiling technologies have promising application prospects in filling the gap left by the loss of spatial information in traditional single-cell sequencing. Within a few years of development, these technologies have made significant progress in resolution and simplicity. This review discusses the development of spatial omics sequencing based on microfluidic array chips, barcoding strategies using various microfluidic designs, and the pros and cons of each strategy. Commercialized solutions for spatial profiling and future research directions are also presented.
Article
Chemistry, Analytical
Wan Zhou, Guanglei Fu, Xiujun Li
Summary: This study introduces nanomaterial-mediated photothermal effects to develop a new type of photothermal bar-chart microfluidic chip (PT-Chip) for visual quantitative detection of biochemicals without the need for bulky and expensive analytical instruments. The PT-Chip was able to simultaneously detect four different human serum samples, showing good analytical performance and a limit of detection of 2.1 ng/mL.
ANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Anyu Wang, Xiao Feng, Guangyun He, Ying Xiao, Tian Zhong, Xi Yu
Summary: Food safety issues are a global concern, and traditional detection methods cannot meet the demands for timely and accurate food safety detection due to their limitations. Digital microfluidic chips offer a promising solution with their ultra-high sensitivity, wide applicability, and real-time detection capabilities.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Zhiguang Wang, Ruipeng Chen, Yue Hou, Yingkai Qin, Shuang Li, Shiping Yang, Zhixian Gao
Summary: In this study, a portable and sensitive method for detecting melamine using stimuli-responsive DNA hydrogels and microfluidic chips was developed. The method utilized melamine aptamer and DNA hydrogel-coated AuNPs for colorimetric detection and quantitative analysis. The combination of microfluidic chips and DNA hydrogels enabled quantitative detection of melamine by taking photos and analyzing the gray value.
ANALYTICA CHIMICA ACTA
(2022)
Article
Materials Science, Multidisciplinary
Santiago Franco Corredor, Fadoua Mayoussi, Manuel Luitz, Andrea Kick, Andreas Goralczyk, David Boecherer, Grace Vera, Dorothea Helmer, Frederik Kotz-Helmer, Bastian E. Rapp
Summary: Polystyrene (PS) is a commonly used material in medical, biological, and biomedical applications due to its beneficial properties. However, rapid prototyping and high-resolution shaping of PS, particularly on a micron scale, are still limited. This study introduces a novel photocurable PS photoresin that enables the fabrication of microfluidic chips with high optical transparency and comparable properties to commercial PS. This method allows researchers in various fields to produce suitable PS structures using commercial equipment.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Chemistry, Multidisciplinary
Na Zhao, Zehua Yu, Jun Huang, Yuxi Liu, Yifan Zhao, Xiangqian Fu, Peihua Yang, Kang Liu
Summary: In this study, a hydrogel-assisted microfluidic chip is proposed for non-invasive monitoring of chemicals in microfluidics. A nanoporous hydrogel acts as a sealing film on top of a microchannel, encapsulating liquid and allowing for non-invasive analysis. This open microchannel can be integrated with various methods for accurate detection, suggesting the potential of hydrogel microfluidic chips for non-invasive clinical diagnostics and smart healthcare.
