Article
Biophysics
Sodikdjon A. Kodirov
Summary: The patch-clamp technique is an advanced method in electrophysiology that allows the study of channel activation within cells and their impact on cell function. With proper parameter inference and recording, patch-clamp research yields reliable results.
BIOPHYSICAL REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Thomas E. Hall, Nicholas Ariotti, Harriet P. Lo, James Rae, Charles Ferguson, Nick Martel, Ye-Wheen Lim, Jean Giacomotto, Robert G. Parton
Summary: Plasma membrane rupture can lead to cell death. This study uses an active trapping model to investigate the structural changes of sarcolemma, showing the essential role of caveolae in maintaining sarcolemmal integrity and quantifying the contribution of caveolar-derived membrane to surface expansion.
Article
Chemistry, Multidisciplinary
Ming Yi, Frederic Heraly, Jian Chang, Atefeh Khorsand Kheirabad, Jiayin Yuan, Yan Wang, Miao Zhang
Summary: The application of organic phosphonic acids as multifunctional spacers in carbide MXene laminates improves permeance, rejection, and stability through the creation of pillared structures. This strategy offers a universal method for regulating transport channels in MXene-based membranes.
CHEMICAL COMMUNICATIONS
(2021)
Article
Engineering, Electrical & Electronic
Weihao Zheng, Yunlong Zhao, Hongcheng Xu, Yangbo Yuan, Weidong Wang, Libo Gao
Summary: We report a stretchable iontronic pressure sensor (SIPS) array with low proximate crosstalk for epidermal monitoring. The SIPS exhibits high sensitivity, low limit of detection, broad linear sensing range, rapid response time, and long-term stable working durability. The serpentine island bridge structure enables the SIPS array to stretch, allowing pulse and grip monitoring of a bent wrist and finger.
IEEE ELECTRON DEVICE LETTERS
(2023)
Article
Biophysics
Yang Yang, Aofei Liu, Ching -Ting Tsai, Chun Liu, Joseph C. Wu, Bianxiao Cui
Summary: The unintended binding of small-molecule drugs to ion channels can cause arrhythmia and heart failure. However, the low throughput of current intracellular recording techniques limits the assessment of drug toxicity. To address this challenge, we have developed the NcEA technology, which enables robust, parallel, and long-duration recording of intracellular action potentials. This technology has the potential to be used in preclinical drug screening.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Plant Sciences
Amit Kumar Goswami, Naveen Kumar Maurya, Suneha Goswami, Kirti Bardhan, Sanjay Kumar Singh, Jai Prakash, Satyabrata Pradhan, Amarjeet Kumar, Viswanathan Chinnusamy, Prabhat Kumar, Radha Mohan Sharma, Stuti Sharma, Deepak Singh Bisht, Chavlesh Kumar
Summary: Low-temperature stress has a significant negative impact on the growth and yield quality of fruit crops. While temperate fruit crops have developed mechanisms to tolerate low temperatures, tropical and sub-tropical fruit crops are more vulnerable to low-temperature stress. Fruit crops respond to low-temperature stress by activating stress-responsive genes, leading to changes in physiological, biochemical, and molecular mechanisms that enhance their tolerance and alleviate damage. However, the understanding of cold tolerance mechanisms is limited to a few fruit crops. Further research is needed to better understand the physiological, biochemical, and molecular components of cold tolerance in fruit crops.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Donald G. Puro
Summary: The preocular tear film is crucial for optimal vision but can be affected by environmental and pathologic conditions. Activation of P2X(7) receptor/channels may impair goblet cell viability, yet the adaptive benefits of this activation are not fully understood. P2X(7) activation can enhance supply of tear-stabilizing mucin but may endanger goblet cell survival.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Physical
LianDong Sha, Jin Lin, Ruomei Qi, Yonghua Song
Summary: In recent years, there has been significant attention on the production of green hydrogen through water electrolysis, with a focus on the dynamic operation of electrolysis stacks. The electric double-layer capacitance (EDLC) is a key factor in determining stack performance and influencing dynamic behavior. High-frequency testing methods commonly used in laboratories are not suitable for cost-efficiency and pose a bottleneck for engineering power electronic devices. This study proposes a novel square-wave pulse current (SPC) method to determine EDLCs of multi-cell electrolysis stacks using industrial rectifiers. Experimental verification on a 2 Nm3/h alkaline water electrolysis stack achieved an accuracy of 2.67%.
JOURNAL OF POWER SOURCES
(2023)
Article
Multidisciplinary Sciences
Etienne de Coulon, Christian Dellenbach, Stephan Rohr
Summary: The LSSE system allows for comprehensive intracellular electrical recordings on cells undergoing strain events, providing stable conditions for studying mechanically activated ion currents. This system offers advanced insights into mechanosensitive ion channel function and allows for quantitative comparisons among different types of channels and cells.
Article
Chemistry, Analytical
Shengjie Chen, Shengsen Zhang, Rong Zhu
Summary: Fast and precise measurements of live single-cell biophysical properties are crucial for disease diagnosis and cytopathologic analysis. A computer vision method is proposed to track cell dielectrophoretic movements on a microchip, enabling efficient and accurate measurement of biophysical parameters and in situ extraction of cell texture features. The method achieves high measurement accuracy and efficiency, as well as high classification accuracies for various cell types.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
William M. Stoy, Bo Yang, Ali Kight, Nathaniel C. Wright, Peter Y. Borden, Garrett B. Stanley, Craig R. Forest
Summary: The new method utilizes the dependency on the distance between cell membrane and glass pipette to develop an autonomous synchronization method for high-yield whole-cell recordings in the deep brain of living mice. This synchronization nearly doubles the gigaseal yield and significantly improves whole-cell recording yield, making pan-brain whole-cell electrophysiology practical in the living mouse brain.
JOURNAL OF NEUROSCIENCE METHODS
(2021)
Article
Biophysics
Pengkun Xia, Jiawei Zuo, Pravin Paudel, Shinhyuk Choi, Xiahui Chen, Md Ashiqur Rahman Laskar, Jing Bai, Weisi Song, JongOne Im, Chao Wang
Summary: Solid-state nanopores have various applications in single-molecule biosensing and sequencing, but high capacitive noise from conventional silicon substrates limits their performance. A new approach using insulating sapphire wafers for nanopore membranes is proposed, demonstrating scalable formation of small membranes with low noise sensing capabilities.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Nanoscience & Nanotechnology
Xiaofeng Luan, Pengbin Liu, Di Huang, Haiping Zhao, Yuang Li, Sheng Sun, Wenchang Zhang, Lingqian Zhang, Mingxiao Li, Tian Zhi, Yang Zhao, Chengjun Huang
Summary: Real-time transformation is crucial for impedance flow cytometry in practice, but the translation of raw data to cellular intrinsic electrical properties is time-consuming. We proposed a fast parallel physical fitting solver that characterizes single cells' electrical properties within 0.62 ms/cell. Based on this solver, we implemented physics-informed real-time impedance flow cytometry (piRT-IFC), which showed comparable speed but higher accuracy than the fully connected neural network predictor. In addition, we demonstrated the advantages of piRT-IFC in handling unfamiliar samples without pretraining.
MICROSYSTEMS & NANOENGINEERING
(2023)
Article
Biology
Paul Pfeiffer, Federico Jose Barreda Tomas, Jiameng Wu, Jan-Hendrik Schleimer, Imre Vida, Susanne Schreiber
Summary: This study introduces a new technique called capacitance clamp, which allows for simulation of altered capacitance in biological neurons. Through mathematical modeling and experimental validation, the feasibility of this technique is demonstrated, providing a new avenue for probing the mechanisms of neuronal signaling.
Article
Neurosciences
Kun Zhang, Yanfei Han, Peng Zhang, Yuqiong Zheng, Aobing Cheng
Summary: The study developed a multiplexed method to measure neurotransmitter dynamics using simultaneous whole-cell patch clamp recordings and genetically encoded fluorescence sensor imaging. Results showed that the genetically encoded sensors and electrophysiological recordings did not interfere with each other, and each technique had its own strengths and weaknesses in detecting different neurotransmitters. This study highlights the potential of combined techniques for future multianalyte monitoring.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)