Review
Biotechnology & Applied Microbiology
Kai Ma, Chun-Liu Mi, Xiang-Xiang Cao, Tian-Yun Wang
Summary: Gene delivery systems are crucial for gene therapy and recombinant protein production, with non-viral vector gene delivery reagents offering advantages such as large packaging load capacity and low immunogenicity. Liposomes and non-liposome cationic polymers are commonly used as gene delivery reagents, with different head structures including quaternary ammonium salt, amine, amino acid, polypeptide, guanidine salt, and a heterocyclic ring.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Daniil V. Gladkikh, Aleksandra V. Sen'kova, Ivan V. Chernikov, Tatyana O. Kabilova, Nelly A. Popova, Valery P. Nikolin, Elena V. Shmendel, Mikhail A. Maslov, Valentin V. Vlassov, Marina A. Zenkova, Elena L. Chernolovskaya
Summary: The study shows that liposome F is not toxic in healthy mice and can accumulate rapidly in tumors with high folate receptor levels. Combined therapy including anti-MDR1 siRNA/F complexes and polychemotherapy can significantly inhibit the growth of multidrug-resistant tumors without inducing liver toxicity.
Article
Pharmacology & Pharmacy
Min Tang, Sho Sakasai, Hiraku Onishi, Kumi Kawano, Yoshiyuki Hattori
Summary: In this study, modified siRNA/cationic liposome complexes with enhanced stability and selective uptake into tumor cells were prepared. The modified complexes showed selective growth inhibition of human nasopharyngeal carcinoma cells and reduced accumulation in mouse lungs. These findings suggest the potential of PEGylated siRNA/cationic liposome complexes as an effective tool for tumor therapy.
JOURNAL OF DRUG TARGETING
(2023)
Article
Pharmacology & Pharmacy
Upendra Chitgupi, Yiru Qin, Sanjana Ghosh, Breandan Quinn, Kevin Carter, Xuedan He, Ulas Sunar, Jonathan F. Lovell
Summary: This study developed folic acid-decorated light-sensitive liposomes for targeted delivery of chemotherapeutic drugs and demonstrated the efficacy of chemophototherapy in treating tumors. In vitro and in vivo experiments showed that the light treatment could delay tumor growth and improve survival rates. Additionally, the results suggested that the folic acid decoration was not essential for the anti-tumor response.
Article
Chemistry, Medicinal
Shuang Wu, Meiyan Liu, Xiang Hu, Chengxi He, Chunyan Zhao, Shuanglin Xiang, Youlin Zeng
Summary: The chemical and physical structure of cationic liposomes significantly affect their gene transfection efficiency; the design of novel cationic liposomes is guided by the structure-function relationship investigation; the study shows that different lipid structures play a crucial role in gene transfection efficiency.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Sepanta Hosseinpour, Chun Xu, Laurence J. Walsh
Summary: This study investigated the impact of photobiomodulation (PBM) on cationic liposome plasmid DNA transfection efficiency and toxicity. The results showed that using 810 nm and 970 nm lasers significantly enhanced GFP transfection efficiency, while 445 nm and 685 nm lasers reduced efficiency. Treatment with 685, 810, and 970 nm lasers at 12 J maintained cell viability and prevented toxicity of cationic liposomes, supporting the concept that PBM using near infrared laser wavelengths can improve transfection efficiency and cell viability in gene therapy.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Muhammad Aiman Irfan Ibrahim, Rozana Othman, Chin Fei Chee, Faisalina Ahmad Fisol
Summary: Targeting folate receptors is a potential solution for addressing low tumor selectivity in conventional chemotherapeutics. Folate-functionalized nanoparticle drug delivery systems show promise due to their advantages, although none have entered clinical trials. In vitro evidence supports their efficacy compared to non-targeting carriers and free drug formulations. However, in vivo biodistribution studies reveal issues with rapid liver uptake and reduced tumor uptake, potentially influenced by the expression of folate receptor β by activated macrophages in the liver, as well as the route of administration and tumor location. Generalizing nanoparticles reported in various publications is challenging due to different formulations, lack of characterization, and experimental settings, making it difficult to determine the accurate factors influencing targeting effectiveness.
Article
Pharmacology & Pharmacy
Die Hu, Shintaro Fumoto, Naoki Yoshikawa, Jianqing Peng, Hirotaka Miyamoto, Masakazu Tanaka, Koyo Nishida
Summary: Cationic lipid-based lipoplexes were prepared with different sizes and incubated with plasmid DNA at different temperatures to investigate the relationship between physicochemical characteristics and transfection efficiency. The diffusion coefficient of liposomes during lipoplex formation was found to strongly correlate with the physicochemical characteristics of lipoplexes, accessibility of plasmid DNA in lipoplexes, and gene expression per metabolic activity. Clathrin-mediated endocytosis was the major route for lipoplexes comprising 100 nm-liposomes, but as liposome size increased, the major route shifted to lipid raft-mediated endocytosis, with macropinocytosis observed for all liposome sizes. The role of reactive oxygen species may depend on liposome size and endocytosis. This study provides valuable information for understanding cationic lipoplex-mediated transfection.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Chaoyu Liu, Yuancai Xie, Xiaohua Li, Xumei Yao, Xuanbin Wang, Min Wang, Zongxian Li, Fengjun Cao
Summary: Polymeric vectors with different functional groups were synthesized and found to have lower cytotoxicity and adjustable transfection efficiency. Increasing the N/P ratio led to enhanced transfection efficiency, with a significant improvement observed in transfection efficiency above specific critical N/P ratios.
MOLECULAR BIOTECHNOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Ali Shakeri-Zadeh, Abolhasan Rezaeyan, Abolfazl Sarikhani, Hamed Ghaffari, Hadi Samadian, Sara Khademi, Habib Ghaznavi, Jeff W. M. Bulte
Summary: Molecular imaging of cancer is a promising field for visualizing, characterizing, and quantifying cancer biology in vivo. Targeted nanoprobes designed for folate receptors show potential for early cancer detection, but face challenges such as non-specific uptake in the liver and spleen.
Article
Biophysics
Bruna G. Carvalho, Bianca B. M. Garcia, Antonio A. Malfatti-Gasperini, Sang W. Han, Lucimara G. de la Torre
Summary: The association between chondroitin sulfate (CS) and cationic liposomes (CLs)/lipoplex (CL-pDNA) was analyzed in this study. The results showed that the electrostatic interaction between CS and CL/CL-pDNA could reorganize the lipid bilayers and form nanoscale vesicles. These findings have implications for the development of novel carriers for drug and gene delivery applications.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Nutrition & Dietetics
Marisa A. Patti, Joseph M. Braun, Tye E. Arbuckle, Amanda J. MacFarlane
Summary: Maintaining optimal folate levels during early gestation helps reduce the risk of neural tube defects. While inadequate folate intake remains a concern, consuming higher than recommended doses of folic acid does not offer much additional benefit. In the context of mandatory fortification, factors such as socioeconomic status, maternal age, parity, and pre-pregnancy BMI are associated with adequate folate intake and reduced risk of neural tube defects.
AMERICAN JOURNAL OF CLINICAL NUTRITION
(2022)
Article
Nanoscience & Nanotechnology
Liefeng Hu, Shuting Li, Yonggang Lv
Summary: In this study, a pH-sensitive linear poly(ortho ester) polycation was successfully prepared and used for gene transfection in neuroma cells. The polymer showed good biocompatibility and highly specified transfection efficiency on human neuroblastoma cells, with serum tolerance and deeper transfection depth. Compared with the control group, this polymer demonstrated great potential in neuroma therapy.
ACS APPLIED NANO MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Anton V. Lopukhov, Zigang Yang, Matthew J. Haney, Tatiana K. Bronich, Marina Sokolsky-Papkov, Elena V. Batrakova, Natalia L. Klyachko, Alexander V. Kabanov
Summary: The use of mannose-decorated polyplexes has shown significantly enhanced gene transfection efficiency in macrophages, especially in the presence of Man-PEG-b-pAsp(DET). While cross-linked polyplexes reduced toxicity, the impact on transfection enhancement was moderate. This suggests that Man-PEG-b-pAsp(DET)/pDNA polyplex may be a potential vector for immune cells-based gene therapy.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Chemistry, Medicinal
Zhongjuan Wang, Shaobin Xu, Hongying Xia, Yanqiu Liu, Bin Li, Yueqin Liang, Zhongkun Li
Summary: Genetic medicines show great potential in treating various diseases, but the development of effective gene delivery carriers remains challenging. In this study, lipid-polymer hybrid nanoparticles (LHNPs) were developed as a novel drug delivery system that combines the advantages of polymeric nanoparticles and liposomes. The alpha-cyclodextrin-conjugated generation-2 polyamidoamine dendrimers-lipids hybrid nanoparticles (CDG2-LHNPs) demonstrated superior stability and transfection efficiency compared to traditional carriers. The mechanism of high transfection efficiency was found to involve clathrin-mediated endocytosis, caveolae-mediated endocytosis, and macropinocytosis. CDG2-LHNPs have the potential to overcome the limitations of liposomes and polymers for gene drug delivery.
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
(2022)
Article
Materials Science, Biomaterials
Y. N. Zhao, Y. Z. Piao, C. M. Zhang, Y. M. Jiang, A. Liu, S. H. Cui, D. F. Zhi, Y. H. Zhen, S. B. Zhang
JOURNAL OF MATERIALS CHEMISTRY B
(2017)
Article
Pharmacology & Pharmacy
Xiaodong Xu, An Liu, Yuchao Bai, Yinan Li, Chuanmin Zhang, Shaohui Cui, Yongzhe Piao, Shubiao Zhang
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2019)
Article
Multidisciplinary Sciences
Huiying Chen, Shaohui Cui, Yinan Zhao, Chuanmin Zhang, Shubiao Zhang, Xiaojun Peng
Article
Biophysics
Yanyan Zhu, Yao Meng, Yinan Zhao, Jie Zhu, Hong Xu, Enxia Zhang, Lei Shi, Linying Du, Guoliang Liu, Chuanmin Zhang, Xiaodong Xu, Xiaohui Kang, Yuhong Zhen, Shubiao Zhang
COLLOIDS AND SURFACES B-BIOINTERFACES
(2019)
Article
Biophysics
Qin-Qin Gao, Chuan-Min Zhang, En-Xia Zhang, Hui-Ying Chen, Yu-Hong Zhen, Shu-Biao Zhang, Shu-Fen Zhang
COLLOIDS AND SURFACES B-BIOINTERFACES
(2019)
Article
Chemistry, Physical
Chuanmin Zhang, Shubiao Zhang, Defu Zhi, Yinan Zhao, Shaohui Cui, Jingnan Cui
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2020)
Article
Pharmacology & Pharmacy
Chuanmin Zhang, Yinan Zhao, Enxia Zhang, Meilin Jiang, Defu Zhi, Huiying Chen, Shaohui Cui, Yuhong Zhen, Jingnan Cui, Shubiao Zhang
Article
Polymer Science
Hui-ying Chen, Shu-biao Zhang, Xiao-jun Peng, Shao-hui Cui, Yi-nan Zhao, Chuan-min Zhang, Ying Han
ACTA POLYMERICA SINICA
(2014)
Article
Chemistry, Medicinal
Shibin Zhao, Julian Maceren, Mia Chung, Samantha Stone, Raphael Geiben, Melissa L. Boby, Bradley S. Sherborne, Derek S. Tan
Summary: Antibiotic resistance is a major threat to public health, with Gram-negative bacteria presenting unique challenges due to their low permeability and efflux pumps. Limited understanding of the chemical rules for overcoming these barriers hinders antibacterial drug discovery. Efforts to address this issue, such as screening compound libraries and using cheminformatic analysis, have led to the design of sulfamidoadenosines with diverse substituents, showing potential utility in accumulation in Escherichia coli.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Jichun Li, Qing Li, Shuai Xia, Jiahuang Tu, Longbo Zheng, Qian Wang, Shibo Jiang, Chao Wang
Summary: This study successfully developed a short peptide mimetic as a MERS-CoV fusion inhibitor by reproducing the key recognition features of the HR2 helix. The resulting 23-mer lipopeptide showed comparable inhibitory effect to the 36-mer HR2 peptide HR2P-M2. This has important implications for developing short peptide-based antiviral agents to treat MERS-CoV infection.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Krista Jaunsleine, Linda Supe, Jana Spura, Sten van Beek, Anna Sandstrom, Jessica Olsen, Carina Halleskog, Tore Bengtsson, Ilga Mutule, Benjamin Pelcman
Summary: Beta(2)-adrenergic receptor agonists can stimulate glucose uptake by skeletal muscle cells and are therefore potential treatments for type 2 diabetes. The chirality of compounds has a significant impact on the activity of these agonists. This study found that certain synthesized compounds showed higher glucose uptake activity. These findings provide important information for the design of novel beta(2)AR agonists for T2D treatment.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Xin Xu, Jia Chen, Guan Wang, Xiaojuan Zhang, Qiang Li, Xiaobo Zhou, Fengying Guo, Min Li
Summary: The study focuses on EZH2, a promising therapeutic target for various types of cancers. Researchers designed and synthesized a series of novel derivatives aiming to enhance the EZH2 inhibition activity. Among them, compound 28 displayed potent EZH2 inhibition activity and showed high anti-proliferative effects in lymphoma cell lines and xenograft mouse models. The study suggests that compound 28 has potential as a therapeutic candidate for EZH2-associated cancers.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Wei Zhang, Wei Liu, Ya-Dong Zhao, Li-Zi Xing, Ji Xu, Rui-Jun Li, Yun-Xiao Zhang
Summary: This study developed a series of aromatic amide derivatives based on Rhein and investigated their inhibitory activity against alpha-Syn aggregation. Two of these compounds showed promising potential in treating Parkinson's disease by stabilizing alpha-Syn's conformation and disassembling alpha-Syn oligomers and fibrils.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Mani Sharma, S. S. S. S. Sudha Ambadipudi, Neeraj Kumar Chouhan, V. Lakshma Nayak, Srihari Pabbaraja, Sai Balaji Andugulapati, Ramakrishna Sistla
Summary: Therapeutically active lipids in drug delivery systems can enhance the safety and efficacy of treatment. The liposome formulation created using synthesized biologically active lipids showed additive anti-cancer effects and reduced tumorigenic potential.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
