期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 199, 期 -, 页码 -出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2019.110779
关键词
Ferrocenyl compounds; Platinum; Hydroxyquinoline derivatives; Trypanosoma brucei; Cancer cells; Anti-proliferative
资金
- Agencia Nacional de Investigation e Innovation (ANII, Uruguay) [POS_NAC_2016_1_129899, POS_NAC_2018_1_151206, POS_NAC_2015_1_110215]
- PEDECIBA Uruguay
- ANII [PRFMV2009_12617]
- FOCEM (MERCOSUR Structural Convergence Fund) [COF 03/11]
- Fundacao para a Ciencia e a Tecnologia [UID/MULTI/04349/2013, UID/BIO/04565/2013]
- [LISBOA-01-0145-FEDER-007317]
Searching for a more effective chemotherapy for the treatment of Human African trypanosomiasis, the disease caused by the parasite Trypanosoma brucei, and cancer, in the current work five new [Pt-II(L)(dppf)](PF6) compounds, with HL = 8-hydroxyquinoline derivatives and dppf = 1,1'-bis(diphenylphosphino)ferrocene, were synthesized and fully characterized. Crystal structures of three compounds were solved by XRD. The compounds displayed fairly good activity against bloodstream T. brucei, with IC50 values in the submicromolar range (IC50: 0.14-0.93 mu M), and good selectivity towards the pathogen (SI: 11- 48) with respect to mammalian macrophages (cell line J774). Coordination to the {Pt-dppf} moiety led, in most cases, to an enhancement of the activity in respect to the bioactive ligands (11 to 41 fold). Cytotoxicity was assessed against wildtype (A2780) and cisplatinresistance (A2780cisR) ovarian cancer cell lines. Four [Pt-II(L)(dppOl(PF6) compounds were more active (IC50: 1.2-4.4 mu M) than cisplatin (IC50: 26.0 mu M) on A2780 cells and showed far superior activity than the reference drug against A2780cisR cells. Platinum levels in A2780 cells showed poor correlation between cellular uptake and the cytotoxic activity. All the complexes interacted with DNA and the most active ones induced reactive oxygen species (ROS) formation which suggested that the mechanism of action for these complexes may be mediated by oxidative stress and interaction with DNA that could act as a potential molecular target for this type of complexes. Some complexes of this series could be considered new hits for the development of prospective agents against trypanosomatid parasites and ovarian cancer.
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