Article
Engineering, Chemical
Xing Yang, Tina Hsia, Andrea Merenda, Riyadh AL-Attabi, Ludovic F. Dumee, San H. Thang, Lingxue Kong
Summary: The purification of biopharmaceutical streams is crucial for the production of high-quality therapeutic bioproducts. In this study, a novel polyacrylonitrile (PAN)-based nanofibrous membrane with strong anion exchange functionality was developed using electrospinning. The resulting composite membranes showed positive surface charge and different surface properties due to changes in surface micro/nano morphology.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Mechanics
Carlos D. Luzi, Osvaldo M. Martinez, Guillermo F. Barreto
Summary: The influence of radial velocity profiles on trans-column dispersion in a packed bed during single-phase flow was investigated. The magnitude of the dispersive effect was evaluated for a long bed, and the results showed satisfactory agreement. It was found that the trans-column dispersion coefficient is affected by the vessel-to-particle diameter ratio.
Article
Biochemical Research Methods
Boxin Chen, Fei Feng, Yangyang Zhao, Qiyong Liu, Bin Zhao, Lei Li, Haimei Zhou, Xinxin Li
Summary: A new type of semi-packed column, SC -HECP, with high-density elliptic cylindrical posts was reported in this paper, showing improvements in surface area and aspect ratio compared to the traditional SC -CP. Experimental results demonstrated better separation performance in SC -HECP due to the increase in surface area and aspect ratio, leading to a significant increase in theoretical plates and decrease in tailing factor for nonane.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Engineering, Chemical
Qirong Ke, Mathias Ulbricht
Summary: This study focuses on the in situ modification of commercial polyethersulfone (PES) microfiltration membranes to efficiently remove boron in seawater desalination. By coating the pore surface with a boron affinity polymer-based hydrogel, the modified membranes showed superior adsorber properties. The trade-off between permeance and boron binding capacity was studied to identify promising materials for competitive overall separation performance.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Article
Environmental Sciences
Rameez Ahmad Aftab, Sadaf Zaidi, Mohd Danish, Sayed Mohammad Adnan, Khursheed B. Ansari, Mohammad Danish
Summary: This study applied support vector regression (SVR) to predict phenol adsorption on activated carbon in RPB, finding that the SVR model and ANN model had similar predictive performance, with the ANN model slightly outperforming the SVR model. In the test dataset, the coefficient of determination (R-2) and root mean square error (RMSE) values for the MR RPB adsorption model were 0.934 and 0.149, while for the SVR and ANN-based models, these values were 0.996 and 0.045, and 0.998 and 0.027, respectively.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Environmental Sciences
Jose M. Obon, Jose A. Fernandez-Lopez, Mercedes Alacid, Jose M. Angosto
Summary: This study compared the performance of a spinning submerged filter adsorber and a fixed bed adsorber in continuous antibiotic removal. It was found that the fixed bed adsorber packed with coarse powder activated carbon was the most efficient adsorber for treating wastewater spiked with high concentrations of antibiotics. The spinning submerged filter adsorber using a lower concentration of powder activated carbon also achieved high removal effectiveness. This adsorption unit can be adapted to the effluent micropollutant concentrations, making it a promising alternative for adsorption processes.
Review
Engineering, Chemical
Xing Yang, Andrea Merenda, Riyadh AL-Attabi, Ludovic F. Dumee, Xiwang Zhang, San H. Thang, Hung Pham, Lingxue Kong
Summary: Membrane chromatography is considered a potential solution for downstream processing in protein purification. Despite limited commercial adoption in large-scale production, significant progress has been made. However, robust evaluation of its performance is still needed, and the disconnection between academic and commercial efforts should be addressed.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Article
Chemistry, Analytical
Bert Vankeerberghen, Francois Parmentier, Gert Desmet
Summary: The efficiency of liquid chromatography separations can be greatly improved by using a bundle of parallel capillary tubes instead of packed bed columns. However, the presence of polydispersity caused by small differences in capillary diameter ruins this potential. The concept of diffusional bridging that introduces a cross talk between adjacent capillaries has been proposed to solve this issue, and this study provides the first experimental proof and validation of the theory behind it. The observed reduction in dispersion aligns well with theoretical predictions, suggesting that this theory could lead to the design of a new generation of chromatographic beds with unprecedented performance.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yuan Huan, Du Xiao-Song, Xu Ming, Zhang Chuan-Wu, Chen Xiang-Dong
Summary: A 200-cm-long, 300 μm-depth and 80 μm-wide gas chromatographic column was fabricated on a silicon chip using MEMS technique. The semi-packed GC column achieved even flow velocity and improved flow uniformity at zones between bends, with a more nonsymmetric distribution at the bends. Benzene and toluene were separated within 1.5 min, and the column achieved a plate number of 4790 plates/m for N-alkane components.
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Edvaldo V. S. Maciel, Joao V. B. Borsatto, Karen Mejia-Carmona, Fernando M. Lancas
Summary: Graphene oxide-based LC stationary phases were developed for separating hormones from urine using capillaryLC-MS/MS. Two stationary phases with different particle diameters (5μm and 10μm) were produced, and the 10μm phase showed better performance. The SiGO-C18 capillary column successfully separated hormones in urine.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Applied
Lucile Lecas, Vincent Dugas, Claire Demesmay
Summary: Affinity chromatography, often overlooked in receptor/ligand studies, offers new perspectives with its simplicity and ability to provide diverse information. Different immobilization strategies have been developed to encompass a wide range of applications, from ligand-ranking to fragment screening and identification of active components from natural extracts.
SEPARATION AND PURIFICATION REVIEWS
(2021)
Article
Engineering, Chemical
Ziqi Wang, Guiru Zhu, Qi Wang, Kaiyue Ding, Yunbo Tong, Congjie Gao
Summary: The improvement of the molding process of adsorbent powders, utilizing the liquid marble phenomenon, leads to the successful preparation of millimeter-sized spherical particles SSG, which shows significant progress in the practical application of uranium adsorption. SSG demonstrates high adsorption capacity and shows behavior consistent with the pseudo-second-order model and Langmuir model. Moreover, SSG proves its capability in uranium extraction, offering a new approach for practical applications.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Engineering, Geological
Andreas Nicolas Berntsen, Euripides Papamichos, Xiyang Xie, Gang Li, Ziheng Yao
Summary: In a cased hole gravel pack completion, good packing of perforations significantly improves productivity by preventing shear failure and erosion. Partial packing can also maintain productivity well, depending on perforation orientation. Compaction failure is unlikely, while damage at the proppant-formation interface or proppant filtering of produced sand are more probable explanations for productivity reduction.
ROCK MECHANICS AND ROCK ENGINEERING
(2023)
Article
Environmental Sciences
Mohd Danish, Khursheed B. Ansari, Mohammad Danish
Summary: In this study, models were used to analyze the adsorption of Pb(II) from an aqueous solution using a nanostructured gamma-alumina adsorbent in a packed bed adsorber. The simulation results successfully predicted various parameters of the adsorption process and were validated using statistical measures.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Materials Science, Multidisciplinary
Zhou Hu, Minchang Wang, Liqiang Zhang, Jiwei Jiao, Daohan Ge
Summary: The paper proposes a hybrid-lattice array of micro post structure to effectively improve the velocity field distribution in the channel of traditional semi-packed micro gas chromatography column. By analyzing the flow field of six types of microarray structures, a triangular-quadrangular hybrid lattice array structure 1 is determined as the most effective in improving the uniformity of the velocity field. This provides a theoretical basis for the structural design of new micro gas chromatographic columns.
ECS JOURNAL OF SOLID STATE SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Matilde De Pascale, Maria Grazia De Angelis, Cristiana Boi
Summary: Mixed Matrix Membrane Adsorbers (MMMA) have been developed to remove urea, creatinine, and uric acid from dialysate water in order to reduce water consumption and enable the development of closed-loop hemodialysis devices.
Correction
Biochemical Research Methods
Cristiana Boi, Andrea Malavasi, Ruben G. Carbonell, Gary Gilleskie
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemistry & Molecular Biology
Eleonora Lalli, Giulio C. Sarti, Cristiana Boi
Summary: Characterization and modeling of membrane chromatography processes require consideration of the axial dispersion coefficient as an intrinsic property of porous media, rather than arbitrary assumptions on pore size distribution. The dispersion coefficient can be easily measured through independent experiments. This paper presents the prediction of breakthrough curves using experimentally obtained data and demonstrates that alternative explanations based on unverified assumptions about pore size distribution are not feasible. In contrast, the axial dispersion coefficient is the only measurable parameter that accounts for all contributions to dispersion in membrane chromatography processes.
Article
Engineering, Chemical
Steffen Fahr, Samantha Ayde Pena-Benavides, Lukas Thiel, Carl Sengoba, Kaan Karacasulu, Nina Ihling, Juan Eduardo Sosa-Hernandez, Gary Gilleskie, John M. Woodley, Roberto Parra-Saldivar, Seyed Soheil Mansouri, Kosan Roh
Summary: This study explores the importance of Mobile on Demand (MOD) vaccine manufacturing units in addressing the production and supply chain issues in the Global South. By designing two units based on yeast and mRNA expression, it enables rapid production of a large number of vaccines daily, with reduced production costs, minimized vaccine wastage, and earlier protection for vulnerable populations.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2022)
Review
Biotechnology & Applied Microbiology
Sara Giancaterino, Cristiana Boi
Summary: Extracellular vesicles (EVs) are naturally secreted nanoparticles by all cell types, and they are being researched as biocompatible carriers for drug delivery due to their beneficial properties. The scientific focus is on developing techniques for isolating EVs that can be produced on an industrial scale and meet clinical requirements. Researchers are exploring alternative sources of EVs, such as plant EVs, and alternative techniques for EVs processing, including membrane-based processes for isolation and purification.
BIOTECHNOLOGY ADVANCES
(2023)
Review
Biotechnology & Applied Microbiology
Joseph Lavoie, Jinxin Fan, Behnam Pourdeyhimi, Cristiana Boi, Ruben G. Carbonell
Summary: Nonwoven membranes, as highly engineered fibrous materials, can be manufactured on a large scale and modified on their surfaces using various chemistries and ligands. They have great potential for the development of novel membranes with unique properties. This article summarizes the methods of manufacturing nonwoven fabrics, reviews the use of nonwoven fabric devices in membrane chromatography, and discusses the challenges to overcome for their application in downstream processing operations.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Review
Biotechnology & Applied Microbiology
Laurie Overton, Cristiana Boi, Shriarjun Shastry, Caroline Smith-Moore, John Balchunas, Deepa Sambandan, Gary Gilleskie
Summary: The manufacturing of gene therapy products is a rapidly growing industry due to its potential in treating rare and complex genetic diseases. However, there is a shortage of skilled staff in this field. To address this, more education and training opportunities are needed, such as the hands-on cGMP Biomanufacturing of Vectors for Gene Therapy course offered by BTEC at NC State.
HUMAN GENE THERAPY
(2023)
Article
Biotechnology & Applied Microbiology
Wenning Chu, Shriarjun Shastry, Eduardo Barbieri, Raphael Prodromou, Paul Greback-Clarke, Will Smith, Brandyn Moore, Ryan Kilgore, Christopher Cummings, Jennifer Pancorbo, Gary Gilleskie, Michael A. Daniele, Stefano Menegatti
Summary: This study introduces peptide ligands that selectively capture AAVs and release them under mild conditions, providing an alternative to commercially available adsorbents based on camelid antibodies. These peptide-based adsorbents efficiently purify AAVs while maintaining high transduction activity.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biochemical Research Methods
Shriarjun Shastry, Wenning Chu, Eduardo Barbieri, Paul Greback-Clarke, William K. Smith, Christopher Cummings, Arianna Minzoni, Jennifer Pancorbo, Gary Gilleskie, Kimberly Ritola, Michael A. Daniele, Thomas F. Johnson, Stefano Menegatti
Summary: This study introduces a new peptide ligand for the purification of AAV, which has better capture capacity and higher product yield. This new ligand can elute under near-physiological conditions, has an extended lifetime, and can capture AAV from different clones/clades.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Engineering, Chemical
Jinxin Fan, Sobhana A. Sripada, Dan N. Pham, Marina Y. Linova, John M. Woodley, Stefano Menegatti, Cristiana Boi, Ruben G. Carbonell
Summary: A high-capacity, multimodal cation exchange chromatographic membrane (MMC-MPCA) was developed using a polybutylene terepthalate (PBT) nonwoven fabric conjugated with 2-mercaptopyridine-3-carboxylic acid (MPCA) ligand. The membrane exhibited significantly higher binding capacities and clearance of impurities compared to commercial multimodal resins. It was also effective in protein elution under mild conditions. Proteomics analysis confirmed the clearance of high-risk impurities from the protein A eluate when using the MMC-MPCA membrane.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Chemistry, Physical
Thomas Fabiani, Eleonora Ricci, Cristiana Boi, Simone Dimartino, Maria Grazia De Angelis
Summary: In this study, a comprehensive computational evaluation was conducted on various adsorbents, demonstrating that fluorine-containing materials in covalent organic frameworks (COFs) exhibit stronger urea adsorption and urea/water selectivity. Two COFs, COF-F6 and Tf-DHzDPr, were further explored through molecular dynamics simulations, confirming the urea binding sites and the contributions of electrostatic and van der Waals interactions. This study provides valuable insights for future experiments and accelerates the development of wearable artificial kidneys.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Engineering, Chemical
Sobhana A. Sripada, Driss Elhanafi, Leonard B. Collins, Taufika I. Williams, Marina Y. Linova, John M. Woodley, Cristiana Boi, Stefano Menegatti
Summary: This study presents the development and validation of a novel adsorbent, PichiaGuard, which is functionalized with peptide ligands and targets the whole spectrum of K. phaffii HCPs for protein purification. The PichiaGuard adsorbent has high HCP binding capacity and successfully purified monoclonal antibodies and fragments, showing its potential for modern biopharmaceutical processing.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Engineering, Chemical
C. Boi, F. Borsetti, T. M. Brugo, M. Cappelletti, M. G. De Angelis, S. Fedi, S. Di Giacomo, T. Fabiani, G. Foli, A. Garelli, U. Genchi, D. Ghezzi, C. Gualandi, E. Lalli, M. Magnani, A. Maurizzi, F. Mazzi, N. Mehrabi, M. Minelli, R. Montalbano, L. Morelli, S. Nici, R. Onesti, A. Paglianti, K. Papchenko, S. Pappalardo, N. F. Parisi, S. Rapino, M. Reggio, M. Roselli, E. Ruggeri, L. Sabatini, E. Saracino, G. E. Scarponi, L. Serra, V. Signorini, A. Storione, M. Torsello, E. Tugnoli, C. M. Vargiu, G. Vidali, F. S. Violante
Summary: The outbreak of the SARS-CoV-2 pandemic revealed the global shortage of surgical masks and personal protective equipment. A multidisciplinary team from the University of Bologna established a laboratory to test surgical masks and found that compliant masks typically have three layers and filtration efficiency depends on mechanisms beyond size exclusion.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)