期刊
BIOMOLECULAR NMR ASSIGNMENTS
卷 10, 期 1, 页码 189-192出版社
SPRINGER
DOI: 10.1007/s12104-015-9664-5
关键词
DCL-1 (CD302); C-type lectin like receptor; Protein NMR; Heteronuclear NMR spectroscopy; Resonance assignment
资金
- Institutional Research Concept of the Institute of Microbiology [RVO61388971]
- Ministry of Education Youth and Sports of the Czech Republic [LO1509]
- European Regional Development Funds [CZ.1.07/2.3.00/20.0055, CZ.1.07/2.3.00/30.0003, CZ.1.05/1.1.00/02.0109]
- Charles University in Prague [GAUK 797213, UNCE 204025/2012]
- Czech Science Foundation [P207/10/1040]
- EU [CZ.2.16/3.1.00/24023]
DCL-1 (CD302) is a single-pass type one transmembrane protein which is predominantly expressed on myeloid cell lines. It possess the ability of endocytosis and is assumed to play a role in cell adhesion and migration. It has been also connected to several illnesses but more on the level of mRNA than on the protein expression level. More interestingly it is alternatively expressed in the form of a fusion protein with another single-pass type one transmembrane protein DEC205 (CD205) which is normally involved in antigen-uptake and endocytosis. The fusion protein has been assigned to have altered function compared to the wild type proteins. We have performed NMR structural analysis of the 16.2 kDa extracellular domain of DCL-1 to get a better insight onto this molecule. We have been able to assign nearly 97 % of resonance frequencies for the N-15 and C-13 labeled recombinant protein. The assignments have been deposited into Biological Magnetic Resonance Data Bank under the accession number 25802.
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