Article
Engineering, Biomedical
Carolina Paganini, Britta Hettich, Marie R. G. Kopp, Adam Eordogh, Umberto Capasso Palmiero, Giorgia Adamo, Nicolas Touzet, Mauro Manno, Antonella Bongiovanni, Pablo Rivera-Fuentes, Jean-Christophe Leroux, Paolo Arosio
Summary: This microfluidic technique allows for rapid detection and quantification of components associated with EV subtypes and impurities, measuring EV purity in minutes with minimal sample handling and less than 5 μL of sample. It accelerates screening of EV samples, aids in evaluating sample reproducibility, and serves as an important complementary tool to current biophysical methods for EV characterization.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Chemistry, Analytical
Kazuhiro Morioka, Hina Sato, Minori Kuboyama, Akio Yanagida, Atsushi Shoji
Summary: The developed handheld fluorescence detection system for capillary-based ELISA successfully minimized background signals and achieved a lower detection limit for resorufin of 0.13 μM. It was also applied to quantify C-reactive protein (CRP) in human serum, with a lower detection limit of 31 ng/ml, showing comparable results to a conventional ELISA system.
Article
Chemistry, Applied
Minglu Wang, Ying Wang, Xiangyang Li, Hongyan Zhang
Summary: The photothermal-sensing microfluidic paper-based analytical chip (PT-Chip) developed in this study enables accurate and sensitive screening of diethylstilbestrol (DES) residue in foods, which is of significant importance for food safety warning.
Article
Biochemical Research Methods
Fatemeh Haghayegh, Razieh Salahandish, Azam Zare, Mahmood Khalghollah, Amir Sanati-Nezhad
Summary: This study develops a self-powered microfluidic chip that can automate the entire electrochemical immuno-biosensing assay, allowing for controlled and sequential delivery of biofluid samples and sensing reagents to the embedded electrochemical biosensor surface.
Article
Chemistry, Analytical
Ali Khodayari Bavil, Drago Sticker, Mario Rothbauer, Peter Ertl, Jungkyu Kim
Summary: An impedimetric biosensor platform was developed to enhance the sensitivity of immunoassays using microparticles as labels by measuring electrical impedance changes. Through testing various microparticles, it was found that magnetic microparticles provided high signal enhancement and stability on the sensor array. By incorporating magnetic microparticles, over ten times improvement of LOD was achieved in sandwich immunoassays.
Article
Chemistry, Analytical
Hamid Aghamohammadi, Seied Ali Hosseini, Sanjana Srikant, Alexander Wong, Mahla Poudineh
Summary: This paper introduces a computational model for the rational design and optimization of immunobead-based assays in microfluidic mixing channels. The model examines various factors affecting the efficiency of analyte capture on the surface of microbeads and is validated experimentally. It can be adapted to a wide range of target molecules and different device designs.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Yuan Zhou, Longping Ma, Lei Luo, Dongliu Xiang, Qing Wang, Yanan Luan, Jin Huang, Jianbo Liu, Xiaohai Yang, Kemin Wang
Summary: A portable method for on-site detection of three mycotoxins was developed using a sonic toothbrush, microfluidic chip, and smartphone. The method allows for complete detection procedures without the need for sophisticated instruments, including sample pretreatment, signal conversion, and processing. The limits of detection for these mycotoxins were lower than the limit values in cereals in the standards of China and the European Union.
CHEMICAL COMMUNICATIONS
(2023)
Review
Chemistry, Analytical
Subramanian Nellaiappan, Pavan Kumar Mandali, Amrish Prabakaran, Uma Maheswari Krishnan
Summary: Human procalcitonin (PCT) serves as a biomarker for severe bacterial infections, sepsis, and potentially COVID-19. Development of electrochemical sensors has enhanced sensitivity and rapid response for PCT detection. Current focus is on finding superior electrode materials and nanointerfaces for the development of portable point-of-care devices.
Article
Engineering, Industrial
Xuefei Guan
Summary: The Sparse Moment Quadrature (SMQ) method is a new technique for quantifying uncertainty in high-dimensional complex computational models. It extends the existing moment quadrature method by incorporating the Smolyak rule to reduce the full tensor formula to a sparse tensor formula. The SMQ method provides a practical solution for uncertainty quantification in high-dimensional problems involving complex computational models.
RELIABILITY ENGINEERING & SYSTEM SAFETY
(2024)
Article
Environmental Sciences
Hamze Dokoohaki, Marissa S. Kivi, Rafael Martinez-Feria, Fernando E. Miguez, Gerrit Hoogenboom
Summary: This study aims to explore and quantify different sources of uncertainties by conducting regional simulations for maize and soybean crops in the U.S. Midwest. The research analyzes the impact of uncertainties related to initial conditions, soil input, meteorological forcing, management practices, and model parameters on crop yields.
ENVIRONMENTAL RESEARCH LETTERS
(2021)
Article
Engineering, Mechanical
Menghao Ping, Xinyu Jia, Costas Papadimitriou, Xu Han, Chao Jiang
Summary: A new Bayesian modeling framework is proposed to account for the uncertainties in model parameters arising from various factors. The framework incorporates uncertainty using a single level hierarchy with Normal distributions. The likelihood function is constructed based on the discrepancy between model predictions and measurements, and the posterior PDF of model parameters depends on the lower two moments of the respective PDFs.
MECHANICAL SYSTEMS AND SIGNAL PROCESSING
(2022)
Article
Chemistry, Analytical
Hilal Torul, Zeynep Caglayan Arslan, Tugba Tezcan, Elif Calik Kayis, Merve Calimci, Aysen Gumustas, Ender Yildirim, Haluk Kulah, Ugur Tamer
Summary: Microfluidics allows for the integration of various laboratory protocols on a single system, offering advantages such as efficient transportation, reduced sample volumes, fast analysis time, and improved sensitivity. The combination of immunoassays and microfluidic technology has great potential as a biosensor system for blood samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Amir Osman Abdelrazig, Bao Thai Tran, Patsamon Rijiravanich, Werasak Surareungchai
Summary: A microfluidic analytical device based on wax-patterned Fusion 5 paper was developed for rapid nucleic acid detection of the main causal agent of chili anthracnose in Asia. The biosensor used DNA-conjugated gold nanoparticles for detection with a detection limit down to 17.42 femtomoles, offering a specific and on-site detection method within 15 minutes. The device shows potential for protein assays and has a significant impact on on-site diagnostics.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Analytical
Hiroto Okuyama, Takanori Tamaki, Yuhei Oshiba, Hiroshi Ueda, Takeo Yamaguchi
Summary: A numerical model was presented to systematically design an improved recognition system for a flow-through biosensor, successfully addressing a bottleneck step in sandwich-type detection and achieving a signal comparable to that of a conventional enzyme-linked immunosorbent assay within a short time. The problem can be solved by adopting appropriate receptors and analytical conditions, fulfilling the sensitivity requirements for POCT.
ANALYTICAL CHEMISTRY
(2021)
Article
Physics, Multidisciplinary
Sameh Kaziz, Yosra Saad, Mohamed Hichem Gazzah, Hafedh Belmabrouk
Summary: In this study, 3D finite element simulations were conducted to investigate the binding reaction kinetics of SARS-CoV-2 S protein and its immobilized antibody in a microfluidic immunoassay. The application of ACET force was found to enhance the binding efficiency by reducing the diffusion boundary layer and shortening the detection time of the biosensor.
EUROPEAN PHYSICAL JOURNAL PLUS
(2022)