Article
Plant Sciences
Cristiane do Carmo Cesario, Jimmy Soares, Jamile Fernanda Silva Cossolin, Allan Victor Martins Almeida, Jose Jovanny Bermudez Sierra, Mauricio de Oliveira Leite, Maria Clara Nunes, Jose Eduardo Serrao, Marcio Aredes Martins, Jane Selia dos Reis Coimbra
Summary: This study describes the biochemical and morphological characteristics of Tetradesmus obliquus BR003 isolated from freshwater reservoirs in Brazil. The strain was found to have high unsaturated fatty acids content, soluble proteins, carotenoids, chlorophylls, and specific chemical elements. The cells of BR003 were elliptically curved, with various organelles present, making it the first combined biochemical and ultrastructural morphological characterization of this strain.
Article
Biotechnology & Applied Microbiology
Yu-Chen Cheng, Shih-Chung Chang
Summary: The study prepared two monoclonal antibodies, 7H6C and YC8, with subtype specificity for H5N8 and H5Nx avian influenza viruses, which can be used for specific detection of HA proteins.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Applied
Sivan Gelley, Hodaya Lankry, Jovana Glusac, Ayelet Fishman
Summary: Results suggest that yeast-derived patatin properties are comparable to those from potatoes, indicating potential use in various plant-based products.
Article
Biochemistry & Molecular Biology
Mahasakthy-Vijeyasri Satyaveanthan, Chyan Leong Ng, Alias Awang, Kok Wai Lam, Maizom Hassan
Summary: This study described the bioinformatic analysis and biochemical characterization of a NADP(+)-dependent farnesol dehydrogenase from the cocoa pod borer (CPB) moth, as well as determined the farnesol analogues that may act as a potent analogue inhibitor for CPB infestations. The findings suggest that hexahydroxyfarnesyl acetone could serve as the most effective active ingredient for future biorational pesticide management for CPB infestation.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ingie Elchennawi, Philippe Carpentier, Christelle Caux, Marine Ponge, Sandrine Ollagnier de Choudens
Summary: Iron-sulfur (Fe-S) clusters are essential for cellular pathways and require proteins to mobilize sulfur and iron for their assembly. The SUF system in Mycobacterium tuberculosis is the primary Fe-S biogenesis system and a potential target for fighting tuberculosis. In this study, two proteins of the Mtb SUF system, Rv1464(sufS) and Rv1465(sufU), were characterized and their interaction and activities in Fe-S biogenesis were investigated.
Article
Biochemistry & Molecular Biology
Pengjiao Hou, Wei Hao, Bo Qin, Mengyun Li, Rong Zhao, Sheng Cui
Summary: Schlafen11 is a well-studied protein that plays important roles in cancer therapy and virus-host interactions. The crystal structure and biochemical characteristics of the N-terminal domain (NTD) of Schlafen11 were determined, revealing its potent RNase activity towards type I and II tRNAs and rRNAs. These findings enhance our understanding of the Schlafen family.
NUCLEIC ACIDS RESEARCH
(2023)
Review
Food Science & Technology
Qiaozhi Zhang, Zhouzhou Cheng, Yanbo Wang, Linglin Fu
Summary: The interactions between dietary proteins and phenolic compounds play a critical role in the physiochemical characteristics, nutritional, and quality attributes of food products. These interactions can impact stability, quality, and bioavailability of bioactive substances in different systems, and can be tailored to develop functional food and nutraceuticals with maximized functionality and quality attributes. The influences of these interactions are largely dependent on the chemical structures, environmental conditions, and preparation steps, highlighting the importance of understanding the mechanisms and applications of protein-phenolic interactions in food science and human nutrition.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2021)
Article
Biochemistry & Molecular Biology
Miquel Saumell-Esnaola, Diego Delgado, Gontzal Garcia del Cano, Maider Beitia, Joan Salles, Imanol Gonzalez-Burguera, Pello Sanchez, Maider Lopez de Jesus, Sergio Barrondo, Mikel Sanchez
Summary: This study explored the molecular and morphological characteristics of platelet-derived exosomes and validated a method for isolating highly pure platelet exosomes. It was found that activated platelets released more exosomes, which exhibited the molecular and morphological features of platelet exosomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Marcus Stabel, Tabea Schweitzer, Karoline Haack, Pascal Gorenflo, Habibu Aliyu, Katrin Ochsenreither
Summary: The study investigates the isolation and identification of five strains of anaerobic fungi, revealing their main metabolic products with a preference for hydrogen, acetate, and formate. Differences in growth and metabolic products on different carbon sources, as well as reactions to elevated sugar concentrations, were discussed.
Article
Chemistry, Medicinal
Zhilin Pan, Baolin Ge, Mingjun Wei, Jeevithan Elango, Wenhui Wu
Summary: This study successfully extracted CII from blue shark cartilage with intact molecular structure. Blue shark cartilage could serve as a potential source for CII extraction with applications in biomedicine.
Article
Food Science & Technology
Nicola Landi, Sara Ragucci, Lucia Citores, Angela Clemente, Hafiza Z. F. Hussain, Rosario Iglesias, Jose M. Ferreras, Antimo Di Maro
Summary: In this study, a group of new ribosome-inactivating proteins (RIPs) called sodins were isolated from Salsola soda L., including five proteins from seeds, one protein from edible leaves, and one protein from roots. The sodins exhibited ribosome-inactivating and protein synthesis inhibiting activities, as well as polynucleotide:adenosine glycosylase activity and cytotoxicity towards cell lines. Particularly, sodin 5 showed antifungal activity against Penicillium digitatum.
Article
Biochemistry & Molecular Biology
Stefania Mitola, Cosetta Ravelli, Michela Corsini, Alessandra Gianoncelli, Federico Galvagni, Kurt Ballmer-Hofer, Marco Presta, Elisabetta Grillo
Summary: In this study, we propose a multi-step approach for the expression and purification of histidine-tagged recombinant dimeric Gremlin-1 protein, which can act as a BMP antagonist and a VEGFR2 agonist, using HEK293T cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Francesco Rinaldi, Fabrizio Schipani, Beatrice Balboni, Federico Catalano, Roberto Marotta, Samuel H. Myers, Viola Previtali, Marina Veronesi, Luigi Scietti, Valentina Cecatiello, Sebastiano Pasqualato, Jose Antonio Ortega, Stefania Girotto, Andrea Cavalli
Summary: This study successfully developed a monomeric recombinant form of human RAD51 and identified the most suitable conditions to stabilize the monomer using different buffers. The monomer of human RAD51 provides a crucial biological tool for investigating RAD51-mediated homologous recombination and paves the way for more reliable studies on structure, mechanism, and drug discovery.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Environmental Sciences
Priyanka Kulkarni, Mithun Maniyar, Megha Nalawade, Prashant Bhagwat, Santhosh Pillai
Summary: Collagen extracted from fish scales showed good solubility and emulsifying potential, making it suitable for the preparation of antimicrobial films. These results suggest that collagen from fish waste can be effectively utilized in the synthesis of novel biodegradable films with antimicrobial properties when combined with chitosan and neem extract.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Microbiology
Francisco Massot, Panagiotis Gkorezis, Jonathan Van Hamme, Damian Marino, Bojana Spirovic Trifunovic, Gorica Vukovic, Jan D'Haen, Isabel Pintelon, Ana Maria Giulietti, Luciano Merini, Jaco Vangronsveld, Sofie Thijs
Summary: The study isolated bacteria from a site chronically exposed to glyphosate, evaluated their plant-colonizing, glyphosate-tolerant, and -degrading abilities, and demonstrated their potential for remediation of glyphosate contaminated agronomic soils through experimental tests.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Raniele Oliveira Alves, Rodrigo Lira de Oliveira, Osmar Soares da Silva, Ana Lucia Figueiredo Porto, Camila Souza Porto, Tatiana Souza Porto
Summary: The study evaluated the influence of PEG molar mass, pH, PEG concentration, and sodium citrate concentration on the extractive fermentation production of protease. The protease demonstrated stability at 60 degrees C, with optimal temperature at 40 degrees C and pH 8.0. It showed metalloprotease characteristics, being enhanced by Fe2+, Ca2+, and Zn2+ ions but inhibited by EDTA.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Jose Manoel Wanderley Duarte Neto, Jonatas de Carvalho Silva, Flavia Sousa, Odete Sofia Lopes Goncalves, Maria Carolina de Albuquerque Wanderley, Bruno Sarmento, Carolina de Albuquerque Lima, Maria Teresa Neves-Petersen, Ana Lucia Figueiredo Porto
Summary: This study characterized a stable extracellular alkaline peptidase produced by Penicillium aurantiogriseum through various methods, revealing its structural and stability characteristics at different pH values. The findings suggest that the protease exhibits higher thermal stability and alpha-helix content at pH 9.0, indicating its potential applicability in practical production.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Alexsandra Frazao de Andrade, Ana Lucia Figueiredo Porto, Raquel Pedrosa Bezerra
Summary: This article discusses the potential of using plant compounds from photosynthetic microorganisms as a source of therapeutic molecules for treating wounds. Cyanophyte Spirulina and Chlorophyte Chlorella are more promising microorganisms due to their beneficial substances. However, there is limited knowledge about the specific functions and characteristics of these substances on the skin.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Microbiology
Arthur Hipolito Pereira Leite, Italo Henrique Alves da Silva, Lorenzo Pastrana, Thiago Pajeu Nascimento, Adriana Maria da Silva Telles, Ana Lucia Figueiredo Porto
Summary: This review provides an overview of different Bacillus species used for the production of fibrinolytic proteases, highlighting purification techniques, biochemical characteristics, enzymatic activity, and toxicological evaluations.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Plant Sciences
Sebastiana Lidielda Albuquerque da Silva, Jhullyrson Osman Ferreira Brito, Sonia Barreto Pereira, Watson Arantes Gama, Wilson Jose da Silva Junior, Ana Maria Benko-Iseppon, Valeria Cassano
Summary: This study investigated the genus Gayralia using ITS sequencing of field collections in tropical northeastern Brazil. The results showed low intraspecific divergence of G. brasiliensis and expanded its distribution to the northeastern Brazilian coast and India.
Article
Chemistry, Applied
Jose Noe da Silva Junior, Ana Carla da Silva, Kamila Kassia dos Santos Oliveira, Leyllane Rafael Moreira, Silvana de Fatima Ferreira da Silva Caires, Aldeni Jose da Silva, Yanara Alessandra Santana Moura, Daniela de Araujo Viana Marques, Raquel Pedrosa Bezerra, Virginia Maria Barros de Lorena, Ana Lucia Figueiredo Porto
Summary: Microalgae, such as Chlorella vulgaris and Tetradesmus obliquus, have been found to have trypanocidal activity against Trypanosoma cruzi without cytotoxic effects, making them potential candidates for the development of natural antichagasic drugs.
NATURAL PRODUCT RESEARCH
(2023)
Article
Biochemical Research Methods
Elizianne Pereira Costa, Romero Marcos Pedrosa Brandao-Costa, Wendell Wagner Campos Albuquerque, Thiago Pajeu Nascimento, Amanda Emmanuelle Sales Conniff, Kethylen Barbara Barbosa Cardoso, Anna Gabrielly Duarte Neves, Juanize Matias da Silva Batista, Ana Lucia Figueiredo Porto
Summary: The aim of this research was to purify and characterize a collagenase from Streptomyces antibioticus that can degrade native and denatured collagen. High collagenase activity (377.5 U/mL) was obtained using anion-exchange chromatography. The collagenase showed stability across a range of pH and temperature, with optimal conditions at pH 7.0 and 60°C. Coffee grounds were found to be an interesting source for obtaining collagenase with anticoagulant activity.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Romario da S. Santana, Felipe de S. Mendes, Barbara J. Paula da Silva, Emerson S. Lima, Thiago P. Nascimento, Marcia N. Carneiro da Cunha, Ana Lucia F. Porto, Maria Francisca S. Teixeira, Rosany P. Carvalho, Waldireny R. Gomes
Summary: This study investigated the production of crude extracts from two species of edible mushrooms through submerged fermentation and evaluated their antioxidant, cytotoxic, and fibrinolytic activities. The results showed that the mushroom extracts had low cytotoxicity, good antioxidant activity, and the supplementation of malt broth favored the production of fibrinolytic proteases.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Beatriz de Aquino Marques da Costa, Ana Claudia Vaz de Araujo, Ligia Maria Goncalves Fernandes, Ana Lucia Figueiredo Porto, Vagne de Melo Oliveira, Tatiana Souza Porto
Summary: Collagenolytic proteases produced by Aspergillus heteromorphus URM0269 were extracted using a PEG/sulfate aqueous two-phase system (ATPS). The enzyme preferentially partitioned to the PEG-rich phase and exhibited good stability at various pH levels.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Tulio A. F. Da Silva, Pablo E. Da C. E. Silva, Thiago P. Nascimento, Romero M. P. B. Costa, Attilio Converti, Ana Lucia F. Porto, Raquel P. Bezerra
Summary: A fibrinolytic enzyme from the microalga Dunaliella tertiolecta was produced, purified, and characterized. The enzyme showed high fibrinolytic activity, stability at a wide pH and temperature range, and belonged to the serine metalloprotease family. These properties make it a potential candidate for industrial applications.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2023)
Article
Multidisciplinary Sciences
Kessia P. S. Souza, Marcia N. C. Cunha, Juanize M. S. Batista, Vagne M. Oliveira, Thiago P. Nascimento, Amanda E. S. Conniff, Romero M. P. B. Costa, Tatiana S. Porto, Camila S. Porto, Ana Lucia F. Porto
Summary: This study compared the production of collagenolytic proteases using different fermentation methods, with solid-state fermentation showing higher yields and better extraction in the aqueous two-phase system.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2022)
Article
Multidisciplinary Sciences
Anamaria S. Diniz, Silvano L. N. Filho, Watson A. Gama, Ariadne N. Moura
Summary: This study evaluated the effects of temporal and vertical variation of the water column on phytoplankton and zooplankton dynamics in two tropical reservoirs in Northeastern Brazil. The results showed that nitrogen directly influenced phytoplankton, and Calanoida copepods demonstrated the ability to adapt to different trophic conditions and phytoplankton compositions.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2022)
Review
Multidisciplinary Sciences
Andreza P. De Amorim, Gabrielly H. Da Silva, Romero M. P. Brandao, Ana Lucia F. Porto, Raquel P. Bezerra
Summary: This review highlighted the potential of algae-derived ACEi peptides as antihypertensive agents, with macroalgae being more commonly studied for their ACEi activity and thermolysin or bromelain hydrolysates showing the highest ACEi activity.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2022)
