Vogesella urethralis sp. nov., isolated from human urine, and emended descriptions of Vogesella perlucida and Vogesella mureinivorans

A novel Vogesella strain, YM-1(T), was recovered from human urine in PR China in 2017. Cells of strain YM-1(T) were Gram-stain-negative, rod-shaped, aerobic, motile, non-spore-forming and poly-beta-hydroxybutyrate-accumulating. The strain contained C-16:1 omega 6c/C-16:1 omega 7c, C-16:0 and C-18:0 omega 7c as major fatty acids; phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified phospholipid as major polar lipids; and ubiquinone-8 as the predominant respiratory quinone. Comparison of 16S rRNA gene sequences indicated that this strain had highest similarities to Vogesella perlucida DS-28(T) (98.8%) and Vogesella mureinivorans 389(T) (98.1%). The results of phylogenetic analysis based on the 16S rRNA gene sequences revealed that the novel strain was clustered and well separated with V. perlucida DS-28(T) and V. mureinivorans 389(T) within the genus Vogesella. The average nucleotide identity (ANI) and amino acid identity (AAI) analyses showed that this strain was not identified as V. perlucida DS-28(T) or V. mureinivorans 389(T), with values well below the threshold limit for species demarcation (ANI <88.1%, AAI <88.6 %). Based on the above results, strain YM-1(T) is proposed to be a novel species of the genus Vogesella with the name Vogesella urethralis sp. nov. (YM-1(T)=NBRC 113779=CGMCC 1.17135).