Article
Biotechnology & Applied Microbiology
Jueun Kim, Yeong-Jin Choi, Honghyun Park, Hui-suk Yun
Summary: Novel alginate-hydroxyapatite hybrid microspheres were developed for simultaneous delivery of drugs and cells for osteoporotic bone tissue regeneration. The microspheres enhanced osteogenesis and carried and delivered quercetin, a phytoestrogen, through sustained release. The presence of hydroxyapatite boosted the osteogenic differentiation behavior of cells encapsulated in the hybrid microspheres. These multi-biofunctional microspheres have great potential for the regeneration of osteoporotic bone tissue.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Physical
Youping Gong, Honghao Chen, Wenxin Li, Chuanping Zhou, Rougang Zhou, Haiming Zhao, Huifeng Shao
Summary: In this study, a multi-channel dynamic micromixer was developed to achieve rapid mixing and fabrication of heterogeneous gel microspheres with adjustable cell concentration. The micromixer demonstrated efficient mixing and enabled gradient printing, reducing the demand for biological reagents and materials. This novel method has the potential to be utilized in various fields such as analytical chemistry, microarray, drug screening, and tissue culture.
Article
Peripheral Vascular Disease
Akram Salami Ghaleh, Sepideh Saghati, Reza Rahbarghazi, Ayla Hassani, Leila Shafiei Kaleybar, Mohammad Hossein Geranmayeh, Mehdi Hassanpour, Jafar Rezaie, Hossein Soltanzadeh
Summary: The study demonstrated that encapsulated human endothelial cells cultured under static conditions showed increased survival rates compared to conventional 2D culture. The dynamic bioreactor system did not affect cell viability. Both dynamic and static culture models enhanced the expression of VEGFR-2 and von Willebrand factor in encapsulated cells, indicating functional maturation. Dynamic encapsulated cells also showed a significant increase in vWF levels and a reduction in apoptosis markers compared to the static culture system. Additionally, both dynamic and static culture models exhibited increased levels of SOD and GPx enzymes, suggesting enhanced antioxidant activity. The in vitro tubulogenesis assay indicated a significant induction of angiogenesis in dynamic encapsulated HUVECs, demonstrating the potential of the bioreactor dynamic system for expanding encapsulated human ECs in a 3D environment.
MICROVASCULAR RESEARCH
(2021)
Article
Chemistry, Physical
Lilith M. Caballero Aguilar, Serena Duchi, Carmine Onofrillo, Cathal D. O'Connell, Claudia Di Bella, Simon E. Moulton
Summary: Microfluidic systems show great potential in delivering bioactive agents for tissue engineering applications. The fine-tuning of parameters in microfluidic systems is crucial for maximizing encapsulation efficiency and generating bioactive microspheres.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Engineering, Biomedical
Fabiola Munarin, Carly Kabelac, Kareen L. K. Coulombe
Summary: A biomimetic approach was developed to enhance the binding affinity of the extracellular matrix for growth factors, allowing for controlled and localized release of proangiogenic cues such as VEGF. The chemically modified alginate microspheres showed sustained release of VEGF and promoted primitive vessel-like network formation, even in the absence of VEGF. These findings suggest that sulfate- and heparin-alginate hydrogels are promising adaptive and bioactive delivery systems for revascularization therapy and translational vascular tissue engineering.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Biochemistry & Molecular Biology
Chenxi Zhang, Yuxin Xu, Shuang Wu, Weiyun Zheng, Shuang Song, Chunqing Ai
Summary: Ax-enriched colon targeted alginate particles (Ax-Alg) with good structural integrity and colon targeting can alleviate ulcerative colitis. Ax-Alg exerts its effects by regulating the gut microbiota composition and releasing ax through gut microbiota fermentation. Ax-Alg shows better therapeutic effect on ulcerative colitis compared to oil-in-water emulsion.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Chemistry, Multidisciplinary
Zhenyu Zhao, Zhen Wang, Gen Li, Zhengwei Cai, Jiezhou Wu, Lei Wang, Lianfu Deng, Ming Cai, Wenguo Cui
Summary: Microfluidic hydrogel microspheres have been widely studied and used in various industries, with a focus on their applications in the medical field for controlled delivery of cells and drugs. These materials enable efficient encapsulation and control of functionalities, promoting collaboration across disciplines and advancing precision medicine and applied materials.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Pharmacology & Pharmacy
Tamara Athamneh, Adil Amin, Edit Benke, Rita Ambrus, Pavel Gurikov, Irina Smirnova, Claudia S. Leopold
Summary: The study utilized aerogel technology to prepare pulmonary drug carriers with high porosity, low density, and large surface area suitable for pulmonary drug delivery, demonstrating a non-Fickian drug release mechanism.
PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
M. Cirri, F. Maestrelli, S. Scuota, V Bazzucchi, P. Mura
Summary: Metronidazole-loaded vaginal multiparticulate systems were developed to improve drug release, with the best formulation demonstrating efficacy in treating local diseases based on various performance tests conducted.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Polymer Science
Maria Inmaculada Garcia-Briega, Joaquin Rodenas-Rochina, Luis Amaro Martins, Senentxu Lanceros-Mendez, Gloria Gallego Ferrer, Amparo Sempere, Jose Luis Gomez Ribelles
Summary: This study enhances the stability of alginate microspheres by grafting specific biomolecules onto their surface to form microgels, mimicking the natural microenvironment. The stability of the microgel in different culture media and the formation of polyelectrolyte multilayers are investigated, and the proliferation of multiple myeloma cells is tested in a bioreactor under gentle agitation.
Review
Biochemical Research Methods
Marta M. Maciel, Tiago R. Correia, Mariana Henriques, Joao F. Mano
Summary: The modulation of cell response in tissue engineering is challenging, but the potential of microparticles in controlling cellular response has been overlooked. However, microparticles designed with biophysical and biochemical cues can effectively modulate cell response.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Concetta Di Natale, Elena Lagreca, Valeria Panzetta, Marianna Gallo, Francesca Passannanti, Michele Vitale, Sabato Fusco, Raffaele Vecchione, Roberto Nigro, Paolo Netti
Summary: The intestinal microbiota, composed of various bacterial species and strains, plays a crucial role in immune system development and function. Probiotics, through modulation of the microbiota, offer potential in the pharmaceutical and healthcare market. Encapsulation strategies have shown promise in protecting probiotic viability, with a need for optimization to ensure stability.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Ole Tietz, Fernando Cortezon-Tamarit, Rod Chalk, Sarah Able, Katherine A. Vallis
Summary: This study identifies a method to deliver antibodies and antibody fragments into the cytosol and nucleus of cells using trimeric cell-penetrating peptides (CPPs). By utilizing these peptides, it overcomes a major obstacle in intracellular targeting, which is the entrapment of biomacromolecules in the endosome.
Article
Multidisciplinary Sciences
Alexandra Mun, Haneen Simaan Yameen, Giora Edelbaum, Dror Seliktar
Summary: This study aimed to create a novel agricultural controlled release (CR) device using alginate hydrogel beads embedded with drug-bearing Polycaprolactone (PCL) microspheres, demonstrating the potential for sustained growth inhibition through the controlled release formulation of Paclobutrazol (PBZ) in oatmeal plants.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Yulong Kong, Kui Zeng, Ying Zhang, Jinning Shao, Jiaqi Yan, Jia-Yu Liao, Wenchao Wang, Xiaoyang Dai, Qinjie Weng, Shao Q. Yao, Su Zeng, Linghui Qian
Summary: The pH-responsive CPDIMD achieves tumor targeting delivery by protonation of imidazole groups within the acidic tumor microenvironment.
CHEMICAL COMMUNICATIONS
(2022)
Article
Physiology
D. Merrill Dane, Cuneyt Yilmaz, Dipendra Gyawali, Rashni Iyer, Jyothi Menon, Kytai T. Nguyen, Priya Ravikumar, Aaron S. Estrera, Connie C. W. Hsia
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2019)
Article
Chemistry, Multidisciplinary
Joshua Owen, Eloise Thomas, Jyothi Menon, Michael Gray, Irini Skaripa-Koukelli, Martin R. Gill, Sheena Wallington, Rebecca L. Miller, Katherine A. Vallis, Robert Carlisle
JOURNAL OF CONTROLLED RELEASE
(2020)
Review
Biotechnology & Applied Microbiology
Duong Le, Dinesh Dhamecha, Andrea Gonsalves, Jyothi U. Menon
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Dinesh Dhamecha, Duong Le, Rachel Movsas, Andrea Gonsalves, Jyothi U. Menon
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2020)
Review
Pharmacology & Pharmacy
Arya Bhushan, Andrea Gonsalves, Jyothi U. Menon
Summary: Breast cancer is a leading cause of cancer-related morbidity and mortality in women worldwide, with early diagnosis and effective treatment being crucial for positive outcomes. Theranostics has emerged as a new paradigm for simultaneous diagnosis, imaging, and treatment of cancers, offering potential for personalized therapy. Nanotheranostics, embedding specific targeting moieties, imaging agents, and therapeutic agents within a single formulation, is an effective treatment approach for breast cancer.
Article
Pharmacology & Pharmacy
Moez Ghumman, Dinesh Dhamecha, Andrea Gonsalves, Lauren Fortier, Parand Sorkhdini, Yang Zhou, Jyothi U. Menon
Summary: Idiopathic pulmonary fibrosis is a serious and fatal disease with unclear etiology and limited treatment options. Current oral therapies can alleviate symptoms but may cause severe side effects, while nanoparticle-based sustained drug delivery strategies offer promising treatment options.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2021)
Article
Materials Science, Biomaterials
Dinesh Dhamecha, Duong Le, Tomali Chakravarty, Kalindu Perera, Arnob Dutta, Jyothi U. Menon
Summary: The study introduces a unique biomimetic hydrogel microwell array platform for generation and stress-free isolation of cancer spheroids. Optimized for high-throughput generation, the platform produces cancer cell spheroids with spherical morphology and hypoxic cores, serving as models for drug research.
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Review
Biotechnology & Applied Microbiology
Kalindu Perera, Ryan Ivone, Evelina Natekin, Cheryl. A. Wilga, Jie Shen, Jyothi U. Menon
Summary: Cartilage defects cause joint pain, swelling, and stiffness, affecting patients' quality of life. Current treatment options have limitations, leading to an increased interest in cartilage tissue engineering. 3D bioprinting is a promising method to generate functional structures for cartilage repair, providing flexibility and support needed for implantation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Kalindu Perera, Dat X. Nguyen, Dingbowen Wang, Aneetta E. Kuriakose, Jian Yang, Kytai T. Nguyen, Jyothi U. Menon
Summary: Two novel theranostic nanoparticle formulations, one pH-responsive and the other pH and thermo-responsive, were developed for anticancer drug delivery and therapeutic efficacy tracking. These formulations demonstrated promising properties as inherently fluorescent, stimuli-responsive platforms for targeted anti-cancer therapy.
PHARMACEUTICAL RESEARCH
(2022)
Article
Biochemical Research Methods
Christopher L. Hemme, Rachel Carley, Arielle Norton, Moez Ghumman, Hannah Nguyen, Ryan Ivone, Jyothi U. Menon, Jie Shen, Matthew Bertin, Roberta King, Elizabeth Leibovitz, Roy Bergstrom, Bongsup Cho
Summary: The Rhode Island IDeA Network of Biomedical Research Excellence Molecular Informatics Core at the University of Rhode Island developed virtual and augmented reality apps to teach biomedical science concepts. The apps were positively reviewed and are being implemented into the curriculum.
Article
Materials Science, Biomaterials
Andrea Gonsalves, Pranjali Tambe, Duong Le, Dheeraj Thakore, Aniket S. Wadajkar, Jian Yang, Kytai T. Nguyen, Jyothi U. Menon
Summary: This study presents a novel in situ-forming nanocomposite hydrogel for localized pH-responsive skin cancer therapy and wound healing, showing excellent swelling and compressive strengths, as well as promising therapeutic efficacy against skin cancer cells and bacteria.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Materials Science, Multidisciplinary
Sivaraj Ramasamy, Dinesh Dhamecha, Kiruthiga Kaliyamoorthi, Archana Sumohan Pillai, Aleyamma Alexander, Premnath Dhanaraj, Jyothi U. Menon, Israel V. Muthu Vijayan Enoch
Summary: This study reports novel Sr-HAp and Sr,Fe-HAp hydroxyapatite nanoparticles for delivering doxorubicin to bone cancer cells, with high drug loading percentage and sustained release at physiological pH. In vitro and in vivo tests demonstrate strong anticancer activity of the drug-loaded nanocarriers, particularly on osteosarcoma cell lines. Additionally, the platinum derivative polymer plays a therapeutic role and enhances the overall effectiveness of the nanohybrid material for osteosarcoma treatment.
MATERIALS ADVANCES
(2021)
Meeting Abstract
Oncology
Martin R. Gill, Jyothi U. Menon, Robert Carlisle, Katherine A. Vallis
Article
Medicine, Research & Experimental
Eloise Thomas, Jyothi U. Menon, Joshua Owen, Irini Skaripa-Koukelli, Sheena Wallington, Michael Gray, Christophoros Mannaris, Veerle Kersemans, Danny Allen, Paul Kinchesh, Sean Smart, Robert Carlisle, Katherine A. Vallis
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)