期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 140, 期 -, 页码 1116-1125出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.08.136
关键词
Polysaccharide; Mytilus coruscus; Ultrasonic-assisted enzymatic extraction; Antioxidant activity
资金
- Zhejiang Provincial Natural Science Foundation of China [LGN18C200006, LGN18C200025]
This study aimed to obtain the purified fractions of Mytilus coruscus polysaccharides (MCPs) and investigate their antioxidant activities. MCPs were prepared through ultrasonic-assisted enzymatic extraction optimized by employing the response surface methodology. A single-factor experiment was conducted using the Box-Behnken design to determine the optimum extraction conditions of MCPs. The ultrasonic power was 60 W, liquid-to-material ratio was 30 mL/g, extraction time was 36 min, extraction temperature was 64 degrees C, enzyme concentration was 3.2%, and polysaccharide extraction yield was 12.86% +/- 0.12%. A novel polysaccharide (MCP1-2) was obtained after the purification with AB-8 macroporous resin, DEAE Sepharose Fast Flow, and Sepharose CL-6B column. The molecular weight of MCP1-2 was estimated to be 134.9 kDa according to highperformance gel permeation chromatography. High-pressure liquid-phase chromatography results showed that MCP1-2 contained mannose, rhamnose, glucuronic acid, glucose, galactose, and L-Fuc at a molar ratio of 1.53:1:4.83:81.82:2.36:1.51. Infrared and NMR spectroscopies confirmed that MCP1-2 possessed alpha- and beta- configurations. The antioxidant activities of MCP1-2 were investigated in vitro, and the results showed that MCP1-2 had good antioxidant activity and can be used as a natural antioxidant in food. (C) 2019 Elsevier B.V. All rights reserved.
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