Article
Agriculture, Multidisciplinary
Andrea Castellaneta, Ilario Losito, Beniamino Leoni, Pietro Santamaria, Cosima Damiana Calvano, Tommaso R. Cataldi
Summary: Microgreens are a special type of vegetal product with potentially high content of various nutrients, including glycerophospholipids. The study found that glycerophospholipids in different microgreens are rich in essential fatty acids, which can serve as additional sources in vegetarian/vegan diets.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Irma Magaly Rivas Serna, Michal Sitina, Gorazd B. Stokin, Jose R. Medina-Inojosa, Francisco Lopez-Jimenez, Juan P. Gonzalez-Rivas, Manlio Vinciguerra
Summary: The study found that good cardiovascular health metrics are associated with a specific pattern of phospho- and sphingolipids. Circulatory lipid profiling may be a potential biomarker to refine cardiovascular health status in primary prevention strategies.
Article
Food Science & Technology
Rui Sun, Tingting Wu, Hao Guo, Jiamin Xu, Jiahui Chen, Ningping Tao, Xichang Wang, Jian Zhong
Summary: Using transactional analysis, lipid profile migration from tilapia muscle to juice during the steaming process was revealed. The developed analysis procedure efficiently decreased workload and eliminated false data, compensating for the current lipidomics method's drawbacks. Results showed significant differences in lipid classes among different steaming times, confirming the lipid migration during the process.
NPJ SCIENCE OF FOOD
(2021)
Article
Food Science & Technology
Cheng Li, Zhiqian Liu, Leah Marett, Jennie Pryce, Simone Rochfort
Summary: Milk is a rich source of lipids, and LC-MS is the predominant technique for lipid identification and quantification. However, no standard methods are currently available for the quantification of milk phospholipids. This study aims to examine the factors influencing the quantification outcome of bovine milk phospholipids and discuss the pros and cons of current LC-MS methods.
Article
Food Science & Technology
Yunfeng Zhang, Min Zhang, Linpei Dong, Jing Chang, Haixing Wang, Qing Shen
Summary: The study successfully extracts and quantifies phospholipid molecular species in the muscular tissue of swimming crab, finding high levels of beneficial phospholipids like eicosapentaenoic acid and plasmalogens. The method used was validated to be efficient and accurate, making the muscular tissue of swimming crab a valuable source of nutrition.
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Sha Yan, Xuan Wang, Minghui Sun, Wenqian Wang, Liming Wu, Xiaofeng Xue
Summary: Royal jelly is a popular nutraceutical with unique lipid composition, including phospholipids and trans-10-hydroxy-2-decenoic acid. This study found that the lipid composition of royal jelly is influenced by its botanical origin.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Spectroscopy
Thomas Zuellig, Harald C. Koefeler
Summary: The rapid growth of lipidomics research in the past decade is closely linked to advancements in mass spectrometry instrumentation. The shift from low resolution to high-resolution lipidomics platforms is a key trend in the field.
MASS SPECTROMETRY REVIEWS
(2021)
Article
Biochemical Research Methods
Eva-Maria Harrieder, Fleming Kretschmer, Sebastian Boecker, Michael Witting
Summary: Metabolomics and lipidomics involve the large-scale analysis of metabolites, requiring advanced analytical methods. Liquid Chromatography-Mass Spectrometry (LC-MS) is a commonly used technique that provides different selectivities in separation and high sensitivity in detection. Due to the huge chemical diversity, there is no single analysis method that can cover the entire range of metabolites or lipids, leading to the use of different separation methods. This review explores the current use of LC-MS in metabolomics and lipidomics using data from public databases, and highlights potential future trends and improvements needed.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Chemistry, Applied
Jie Wang, Jie Liao, Honghai Wang, Xiaofang Zhu, Linqiu Li, Weibo Lu, Gongshuai Song, Qing Shen
Summary: A method for analyzing plasmalogens in shellfish was developed, with mussel identified as having the highest content and being a good choice for large-scale preparation.
Article
Biochemical Research Methods
Ines M. S. Guerra, Luisa Diogo, Marisa Pinho, Tania Melo, Pedro Domingues, M. Rosario Domingues, Ana S. P. Moreira
Summary: Phenylketonuria (PKU) is a metabolic disorder caused by impaired function of phenylalanine hydroxylase. Treatment involves restricting phenylalanine intake through dietary modifications. Analysis showed significant differences in plasma phospholipid composition between PKU children and healthy children, with higher levels of phosphatidylcholines containing polyunsaturated fatty acids (PUFA) in PKU group.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Domenico Masuero, Domen Skrab, Giulia Chitarrini, Mar Garcia-Aloy, Pietro Franceschi, Paolo Sivilotti, Graziano Guella, Urska Vrhovsek
Summary: A new mass spectrometry method was developed in this study, successfully analyzing 412 compounds in grapes. The results showed that certain lipid components were positively correlated with grape maturity, while others were negatively correlated.
Article
Food Science & Technology
Yiwei Cui, Honghai Wang, Qiaoling Zhao, Xiaofang Zhu, Pingya Wang, Jing Xue, Kang Chen, Qing Shen
Summary: A novel in-situ method using rapid evaporative ionization mass spectrometry (REIMS) with an electric heating probe was developed for real-time detection of authenticity and adulteration of krill phospholipids with soybean phospholipids. Commercial samples could be directly analyzed without preparation, and accurate differentiation was achieved using characteristic ions.
Article
Food Science & Technology
Silvia Guazzotti, Cristina Pagliano, Francesco Dondero, Marcello Manfredi
Summary: This study evaluated the lipidomic profile of rice bran from the Italian rice variety, Roma, using ultra performance liquid chromatography-tandem mass spectrometry. Ethanol and methyl tert-butyl ether/methanol solvents were found to be excellent solvents for lipid extraction from rice bran, with ethanol being the most effective for triglycerides and N-acylethanolamines, and methyl tert-butyl ether/methanol being the most effective for diglycerides, phospholipids, and ceramides. Additionally, extraction with ethanol at 20 degrees C yielded similar results to extraction at 4 degrees C. Therefore, ethanol and methyl tert-butyl ether/methanol can be used to efficiently extract lipids from rice bran, contributing to the valorization of this by-product in a circular economy perspective.
Article
Ophthalmology
Igor A. Butovich, Tomo Suzuki
Summary: The study found that chronological aging itself seems to have only minor effect on meibogenesis in healthy, non-MGD/non-DE subjects.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Chemistry, Applied
Jie Chen, Qing Kong, Ziting Sun, Jiayuan Liu
Summary: The study using liquid chromatography-mass spectrometry found that lipid composition in salmon muscle changes over time when stored at 4 degrees Celsius, with four lipids showing significant increase from the tenth day. Traditional freshness index also indicated spoilage of salmon slices by the tenth day, suggesting they may serve as indicators of raw salmon freshness.
Review
Biochemistry & Molecular Biology
Wided Najahi-Missaoui, Robert D. Arnold, Brian S. Cummings
Summary: The field of nanotechnology has rapidly grown over the past two decades, leading to the emergence of nanoparticles as promising tools in various applications. However, questions about the toxicity and safety of nanoparticles remain unanswered, despite efforts to understand them. Several approaches to minimize and prevent nanoparticle toxicity have been identified through research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nutrition & Dietetics
Andrew D. Fruge, Kristen S. Smith, Aaron J. Riviere, Rachel Tenpenny-Chigas, Wendy Demark-Wahnefried, Anna E. Arthur, William M. Murrah, William J. van der Pol, Shanese L. Jasper, Casey D. Morrow, Robert D. Arnold, Kimberly Braxton-Lloyd
Summary: Increased intake of green leafy vegetables may reduce the risk of DNA damage and colorectal cancer caused by red meat. In a 12-week intervention trial, it was found that plasma Vitamin K1 levels increased, while levels of 8OHdG and TNF alpha decreased during the green leafy vegetable intervention. Further research is needed to explore the effects of increased green leafy vegetable intake on colorectal cancer risk.
Article
Dermatology
Arden M. Klinczar, Joel D. Griffies, Fiona L. Bateman, Robert D. Arnold, Shanese L. Jasper, Austin R. Brown
Summary: The study aimed to determine the stability of amikacin at 10 and 30 mg/mL concentrations in four topical solutions over a 56 day period. The results showed that amikacin maintained stability within TE, TC, NA, and ND solutions over 56 days, except when formulated at 10 mg/mL within TE.
VETERINARY DERMATOLOGY
(2022)
Article
Engineering, Biomedical
Nicole L. Habbit, Benjamin Anbiah, Luke Anderson, Joshita Suresh, Iman Hassani, Matthew Eggert, Andrew Brannen, Joshua Davis, Yuan Tian, Balabhaskar Prabhakarpandian, Peter Panizzi, Robert D. Arnold, Elizabeth A. Lipke
Summary: In this study, a three-dimensional in vitro prostate cancer tissue model was established and characterized based on xenograft tumor stiffness. The model can mimic the progression of prostate cancer and provide a foundation for future studies on cancer progression and drug development.
ACTA BIOMATERIALIA
(2022)
Article
Pharmacology & Pharmacy
Hamad Alrbyawi, Ishwor Poudel, Manjusha Annaji, Sai H. S. Boddu, Robert D. Arnold, Amit K. Tiwari, R. Jayachandra Babu
Summary: In this study, a pH-sensitive liposomal system was used to deliver daunorubicin to melanoma cells, resulting in enhanced cytotoxic effects. The liposomes exhibited high drug encapsulation efficiency and rapid release at acidic pH, leading to significantly higher cytotoxicity compared to conventional daunorubicin or liposomes.
Article
Biochemistry & Molecular Biology
Hamad Alrbyawi, Sai H. S. Boddu, Ishwor Poudel, Manjusha Annaji, Nur Mita, Robert D. Arnold, Amit K. Tiwari, R. Jayachandra Babu
Summary: This study developed a co-delivery system of daunorubicin and cardiolipin encapsulated in thermosensitive liposomes for the treatment of breast cancer. The liposomes exhibited high drug encapsulation efficiency, small size, and rapid release under mild hyperthermia, resulting in significantly higher cytotoxicity against breast cancer cells compared to daunorubicin or conventional liposomes. This research lays the foundation for future development of liposomal co-delivery systems for breast cancer therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Reproductive Biology
Jean M. Feugang, Ghassan M. Ishak, Matthew W. Eggert, Robert D. Arnold, Orion S. Rivers, Scott T. Willard, Peter L. Ryan, Eduardo L. Gastal
Summary: Despite advancements in assisted reproductive techniques, there is still a need for rapid and minimally invasive in situ approaches to enhance female fertility. Researchers synthesized clinically relevant liposome nanoparticles and injected them into ovarian follicles of mares, achieving successful intrafollicular binding and migration of liposomes between different cell layers. Furthermore, the potential for intracellular delivery of nanoparticles was confirmed in porcine follicle wall cell types.
Article
Biochemistry & Molecular Biology
Ishwor Poudel, Manjusha Annaji, Fajar Setyo Wibowo, Robert D. Arnold, Oladiran Fasina, Brian Via, Vijaya Rangari, Maria Soledad Peresin, Forrest Smith, Muralikrishnan Dhanasekaran, Amit K. Tiwari, R. Jayachandra Babu
Summary: This study investigates the preparation of an inclusion complex of Hispolon with Sulfobutylether-beta-cyclodextrin (SBE beta CD) and the formulation of Hispolon-SBE beta CD trapped within sterically stabilized liposomes (HSC-SL). The results show that HSC-SL exhibits enhanced anticancer activity against melanoma cell lines and sustained drug release compared to Hispolon liposomes and Hispolon-SBE beta CD liposomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Suman Mazumder, Taraswi Mitra Ghosh, Ujjal K. Mukherjee, Sayak Chakravarti, Farshad Amiri, Razan S. Waliagha, Farnaz Hemmati, Panagiotis Mistriotis, Salsabil Ahmed, Isra Elhussin, Ahmad-Bin Salam, Windy Dean-Colomb, Clayton Yates, Robert D. Arnold, Amit K. Mitra
Summary: Prostate cancer (PCa) is a major cause of cancer deaths in US men. Drug resistance and specific cancer subtypes, such as epithelial-to-mesenchymal transdifferentiation (EMT) and neuroendocrine PCa (NEPC), pose significant challenges in PCa treatment. In this study, a computational prediction algorithm called secDrug identified potential secondary drugs for advanced-stage PCa. The proof-of-concept drug FK866, which targets NAMPT, showed promise in inhibiting drug resistance and stemness in PCa. The study also demonstrated the effects of FK866 on tumor metastasis, cell migration, and invasion, as well as regulation of genes associated with cancer stemness. The development pipeline introduced in this study has the potential to improve the management of lethal PCa.
Article
Biochemistry & Molecular Biology
Joshua T. T. Davis, Taraswi Mitra Ghosh, Suman Mazumder, Amit Mitra, Richard Curtis Bird, Robert D. D. Arnold
Summary: Maximum tolerable dosing (MTD) has been the traditional standard for aggressive cancers, but alternative dosing strategies are gaining attention due to their improved toxicity profiles and unique mechanisms. This study investigated whether extended exposure (EE) topotecan could prevent drug resistance and improve long-term drug sensitivity. The findings showed that EE topotecan had a higher barrier to resistance compared to MTD topotecan, maintaining consistent efficacy and a less aggressive malignant phenotype. Transcriptomic analysis revealed differences in epithelial-mesenchymal transition (EMT), efflux pumps, and topoisomerases between the two dosing strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Veterinary Sciences
Rebecca Kennerly, Amanda E. Coleman, Shanese L. Jasper, Robert D. Arnold, Benjamin M. Brainard
Summary: This study evaluated the effect of orally administered pimobendan on platelet function in healthy adult cats. The results showed that pimobendan did not have a significant inhibitory effect on platelet function in cats at two clinically relevant dosages.
AMERICAN JOURNAL OF VETERINARY RESEARCH
(2023)
Article
Veterinary Sciences
Annabelle Vigneault, Natalie D. Mylniczenko, Robert D. Arnold, Shanese L. Jasper-Trotter, Noemie Summa, Catherine Rousseau, Claire Vergneau-Grosset
Summary: Two adult male leopard sharks (Triakis semifasciata) under managed care were diagnosed with suspected dilated cardiomyopathy. Treatment with oral pimobendan resulted in improved cardiac function, increased appetite, and weight gain in the sharks. Long-term use of pimobendan did not cause any adverse effects.
JOURNAL OF ZOO AND WILDLIFE MEDICINE
(2023)
Article
Medicine, Research & Experimental
Ishwor Poudel, Manjusha Annaji, Chu Zhang, Peter R. Panizzi, Robert D. Arnold, Amal Kaddoumi, Rajesh H. Amin, Seungjong Lee, Nima Shamsaei, R. Jayachandra Babu
Summary: A surgically implantable device is a necessary treatment option for millions worldwide with orthopedic injuries. The use of additive manufacturing has enabled the customized fabrication of orthopedic implants with surface modifications. This study investigates the application of laser powder bed fusion to create 3D-printed implants functionalized with antimicrobial coatings to treat postsurgical infections.
MOLECULAR PHARMACEUTICS
(2023)
Article
Engineering, Biomedical
Nicole L. Habbit, Benjamin Anbiah, Joshita Suresh, Luke Anderson, Megan L. Davies, Iman Hassani, Taraswi M. Ghosh, Michael W. Greene, Balabhaskar Prabhakarpandian, Robert D. Arnold, Elizabeth A. Lipke
Summary: Using a 3D-engineered prostate cancer (PCa) tissue model, this study reveals that the incorporation of fibroblasts promotes PCa aggression by increasing proliferation, significant matrix remodeling, and enrichment of tumorigenic hallmark gene sets. Fibroblasts play an elevated role in indolent disease states and may contribute to the switch from androgen-dependent to castration-resistant PCa.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Elizabeth W. Covington, Shaneese L. Jasper-Trotter, Robert D. Arnold, Raj Amin, Susan Egbert, Allison Chung
Summary: High-dose vitamin D3 supplementation (10 000 IU/kg, max. 400 000 IU) significantly increased 25(OH)D3 levels in critically ill pediatric patients.
FUNDAMENTAL & CLINICAL PHARMACOLOGY
(2023)