Article
Plant Sciences
Dongxiao Li, Guangying Ye, Jie Li, Zhenqin Lai, Siyou Ruan, Qi Qi, Zaihua Wang, Sujuan Duan, Hong-Lei Jin, Hong-Bin Wang
Summary: Dendrobium officinale, an edible plant, has medicinal and ornamental functions. Polysaccharides and flavonoids, including anthocyanins, are important components that determine its nutritional quality and consumer appeal. Understanding the molecular mechanisms regulating anthocyanin and polysaccharide biosynthesis can enhance the quality and market value of D. officinale.
Article
Biochemistry & Molecular Biology
Mingze Zhang, Zhenming Yu, Danqi Zeng, Can Si, Conghui Zhao, Haobin Wang, Chuanmao Li, Chunmei He, Jun Duan
Summary: Dendrobium officinale plants adapt to salt stress by enhancing the biosynthesis of secondary metabolites, including key substances such as jasmonic acid and flavonoids.
Article
Plant Sciences
Zhengfei Luo, Lian Liu, Qiong Nie, Mingjin Huang, Chunlii Luo, Yedong Sun, Yongyan Ma, Jianxin Yu, Fuqiang Du
Summary: This study analyzed the secondary metabolites and antioxidant activity of D. officinale from three regions using a metabolomics approach. A total of 411 metabolites were identified, including 136 differential metabolites. These differential metabolites were mainly enriched in secondary metabolic pathways, particularly flavonoids, phenolic acids, and amines. Furthermore, correlation analysis revealed that 48 differential metabolites were significantly positively correlated with antioxidant capacity, with flavonoids being the main influencing factors. These findings provide valuable guidance for variety screening, quality control, and product development of D. officinale.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Yingdan Yuan, Jiajia Zuo, Hanyue Zhang, Mengting Zu, Maoyun Yu, Sian Liu
Summary: This study examined the molecular regulation mechanism of flavonoid accumulation in Dendrobium officinale through RNA-sequencing and metabolome analysis. The results showed that flavonol metabolites were significantly increased in biennial samples, and 30 different genes involved in flavonoid synthesis were identified.
Article
Pharmacology & Pharmacy
Ming Lv, Qingqing Liang, Xiaofang He, Xiaocui Du, Yuhan Liu, Yan Liu, Chongye Fang
Summary: This study investigated the hypoglycemic effect and mechanism of D. officinale leaves. The results showed that D. officinale leaves significantly lowered blood glucose levels in mice on a high-fat diet, potentially by activating the insulin signaling pathway. Further examination revealed that the >3 kDa ethanol-soluble fraction of D. officinale leaves played a major role in lowering blood glucose.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Genetics & Heredity
Yaling Chen, Benchang Hu, Fantao Zhang, Xiangdong Luo, Jiankun Xie
Summary: Dendrobium officinale is a rare and traditional medicinal plant with high pharmacological and nutritional value. Self-incompatibility mechanism is gametophyte self-incompatibility. Transcriptome analysis revealed a large number of differentially expressed genes in self-pollination and cross-pollination samples, showing that self-incompatibility in D. officinale involves multiple genes and pathways.
Article
Biochemistry & Molecular Biology
Yajuan Wang, Ning Jia, Peiyu Wang, Jiameng Liu, Jing Sun, Wei Ye, Bei Fan
Summary: This study comprehensively analyzed the metabolites and transcriptomes of four Dendrobium species, revealing a positive correlation between the expression levels of structural genes and the accumulation of flavonols in different species. The results deepen the understanding of the molecular mechanism of flavonoid biosynthesis in Dendrobium and provide novel insights into the synthesis and accumulation of flavonoids in Dendrobium.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Zhaojian Wang, Weimin Jiang, Yingying Liu, Xiaoxi Meng, Xinglong Su, Mengyang Cao, Liping Wu, Nianjun Yu, Shihai Xing, Daiyin Peng
Summary: Protocorm-like bodies (PLBs) of Dendrobium officinale were obtained through tissue culture. The optimal medium formula for PLB propagation was determined. Stems, PLBs, and leaves of D. officinale had the highest content of different bioactive ingredients. Comparative transcriptome analysis revealed differential gene expression related to biosynthetic pathways of polysaccharides, alkaloids, and flavonoids in D. officinale.
Article
Chemistry, Applied
Shengchang Tao, Yuchen Song, Shaobo Ding, Ruirong He, Qiankun Shi, Fang Hu
Summary: Photodynamic therapy (PDT) eradicates tumors by generating toxic reactive oxygen species (ROS) using a photosensitizer (PS) and appropriate light. We enhanced the immune response to tumors after PDT by using a biocompatible herb polysaccharide as the carrier of the PS. The use of Dendrobium officinale polysaccharide (DOP) and positively charged nanoparticles significantly improved the immune response after PDT.
CARBOHYDRATE POLYMERS
(2023)
Article
Horticulture
Yang Liu, Shan-De Duan, Yin Jia, Li-Hong Hao, Di-Ying Xiang, Duan-Fen Chen, Shan-Ce Niu
Summary: Wild D. officinale is endangered, while cultivated D. officinale has inferior quality and low medicinal components. In this study, tetraploids of D. officinale were successfully generated using colchicine treatment. Morphological observations showed that tetraploids had smaller size, different leaf shape, and larger stem diameter. Cytological observations revealed larger stomata and fewer stomata per unit area in polyploid plants. Chromosomal observations showed that treatment with 0.2% colchicine for 48 hours resulted in the highest tetraploid induction rate.
Article
Biochemistry & Molecular Biology
Wenbo Shu, Meirong Shi, Qiqi Zhang, Wenyu Xie, Liwei Chu, Mingxuan Qiu, Linyan Li, Zhixin Zeng, Lei Han, Zhenyuan Sun
Summary: In this study, two species of Dendrobium were selected to investigate the differences in flower color formation during vernalization. Metabolic profiling and transcriptome sequencing revealed that many differentially accumulated metabolites and differentially expressed genes were enriched not only in the general pathway of flavonoid biosynthesis, but also in multiple subpathways. Putrescine hydroxycinnamoyl transferase 1 (LOC110093422) was identified as a main gene responsible for the differences in flavonoid accumulation during vernalization. This study provides insights into the molecular mechanisms of flavonoid synthesis during vernalization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Wei Zhao, Ji Li, Cheng Zhong, Xuyu Zhang, Yixi Bao
Summary: A novel gold nanoparticle Do-AuNP was synthesized from traditional Chinese medicine Dendrobium officinale extracts without using extra chemicals, showing better anti-tumor efficiency without increasing toxicity in both in vitro and in vivo studies. This study provides valuable information for Do-AuNP as a new nanomedicine for liver cancer.
Article
Food Science & Technology
Ju Ye, Di Wu, Xingdong Wu, Lin Qin, Mengting Yang, Yan-liu Lu, Dao-peng Tan, Yu-qi He
Summary: This study demonstrated that the Dendrobium officinale aqueous extract (DOAE) has a protective effect on acute alcoholic liver injury (AALI) by altering bile acid (BA) synthesis and metabolism, thus reducing the elevation of serum biochemical indicators. These findings suggest that BA metabolism may be a potential target for the protective effect of DOAE.
Article
Chemistry, Medicinal
Yi-Fang Chen, Ji-Feng Luo, Yan-Ni Zhang, Yue-Hu Wang
Summary: Four flavonoid C-glycosides, including two new diglycosides, were isolated from the leaves of Dendrobium officinale. One of the compounds, apigenin 6-C-beta-D-glucopyranosyl-(1 -> 2)-alpha-L-arabinopyranoside, showed inhibitory activity against tyrosinase.
CHEMISTRY OF NATURAL COMPOUNDS
(2023)
Article
Horticulture
Yifan Zhang, Wenwen Huo, Jiayi Hou, Lei Liu, Xiaoying Yu, Lu Xu
Summary: This study found that co-culturing with Tulasnella calospora significantly improves the growth and cultivation of Dendrobium officinale, increasing resistance indicators and the content of functional components.
Article
Plant Sciences
Jianmin Yue, Yanhuang You, Liang Zhang, Zhiyuan Fu, Jinping Wang, Jinchi Zhang, Robert D. Guy
JOURNAL OF PLANT GROWTH REGULATION
(2019)
Article
Forestry
Jinping Wang, Zhiyuan Fu, Qiong Ren, Lingjun Zhu, Jie Lin, Jinchi Zhang, Xuefei Cheng, Jieyi Ma, Jianmin Yue
Article
Agronomy
Cuiyu Liu, Yujie Zhao, Xueqing Zhao, Jinping Wang, Mengmeng Gu, Zhaohe Yuan
Article
Plant Sciences
Jinping Wang, Lu Zhai, Jieyi Ma, Jinchi Zhang, G. Geoff Wang, Xin Liu, Shuifeng Zhang, Juan Song, Yingkang Wu
Article
Forestry
Jinping Wang, Shilin Ma, G. Geoff Wang, Linhao Xu, Zhiyuan Fu, Juan Song, Jinchi Zhang
Summary: This study investigated the occurrence, community composition, and diversity of arbuscular mycorrhizal fungi (AMF) associated with common wild plants on a coastal beach in North Jiangsu, China. Thirty-seven AMF species were isolated from the rhizosphere, with Glomus being the dominant genus and Funneliformis mosseae the dominant species. Soil factors such as sodium ions and pH significantly affected the colonization rate and spore density of AMF, while a positive correlation was found between species richness and total organic carbon.
JOURNAL OF FORESTRY RESEARCH
(2021)
Article
Forestry
Xin Liu, Xuefei Cheng, Nan Wang, Miaojing Meng, Zhaohui Jia, Jinping Wang, Shilin Ma, Yingzhou Tang, Chong Li, Lu Zhai, Bo Zhang, Jinchi Zhang
Summary: The shear strength of soil is significantly influenced by different types of vegetation, leading to variations in shear resistance indices among different stands. Broad-leaved forests have the highest internal friction angle and the lowest cohesion, while coniferous broad-leaved mixed forests have higher soil moisture content and porosity. Root characteristics and soil particle size indirectly affect soil water content, ultimately impacting shear strength.
Article
Plant Sciences
Jinping Wang, Jihong Yuan, Qiong Ren, Bo Zhang, Jinchi Zhang, Rongzhen Huang, G. Geoff Wang
Summary: The study found that arbuscular mycorrhizal fungi (AMF) can enhance the salinity tolerance of Gleditsia sinensis in saline and coastal areas. This is achieved by enhancing stomatal conductance, promoting photosynthesis, increasing antioxidant enzyme activity and membrane stability, reducing harmful substances, and regulating ion balance.
PLANT GROWTH REGULATION
(2022)
Article
Microbiology
Qiong Ren, Jihong Yuan, Jinping Wang, Xin Liu, Shilin Ma, Liyin Zhou, Lujun Miao, Jinchi Zhang
Summary: This study explored the response of soil microbial communities in the Poyang Lake wetland to water level and vegetation type, and revealed the significant impact of water level on soil organic carbon characteristics. Furthermore, water level and vegetation type were found to significantly influence the diversity and community structure of bacteria and fungi. Soil water content, soluble organic carbon, C/N ratio, and microbial biomass carbon were identified as key factors affecting the wetland microbial community.
Article
Ecology
Xin Liu, Shilin Ma, Zhaohui Jia, Muhammad Ramzan, Miaojing Meng, Jinping Wang, Chong Li, Yinlong Zhang, Jinchi Zhang
Summary: The effects of simulated sulfuric, nitric, and mixed acid rain on the root systems of Quercus acutissima and Cunninghamia lanceolata were investigated. The study found that different tree species had varied responses to acid rain, with the sulfur/nitrogen ratio being an important factor affecting plant root growth. This research may contribute to new strategies for the cultivation and protection of plantations in the future.
ECOLOGICAL PROCESSES
(2022)
Article
Forestry
Shilin Ma, Lingjun Zhu, Jinping Wang, Xin Liu, Zhaohui Jia, Chong Li, Jing Liu, Jingyi Zeng, Jinchi Zhang
Summary: This study investigates the effects of different arbuscular mycorrhizal fungi (AMF) and NaCl levels on the growth and physiological responses of Gleditsia sinensis Lam., finding that inoculations with Funneliformis mosseae and Corymbiglomus tortuosum significantly improve root dry weight, morphology, and nutrient content. The results suggest that the use of suitable AMF, such as Funneliformis mosseae, may effectively enhance the production of economic tree species in saline soils, optimizing the utilization of these areas.
Article
Forestry
Jinping Wang, Rongzhen Huang, Liqin Zhu, Hongzhi Guan, Lijing Lin, Huanying Fang, Mengjia Yang, Shaohui Ji, Xianhua Zou, Xin Li
Summary: The study found that the bacterial diversity in biological soil crusts is high, dominated by Acidobacteria, Proteobacteria, Actinobacteria, and Chloroflexi. Below the BSCs, biochar addition has some impact on soil bacterial communities but minimal effect on soil fungal communities.
Article
Forestry
Shilin Ma, Jianmin Yue, Jinping Wang, Zhaohui Jia, Chong Li, Jingyi Zeng, Xin Liu, Jinchi Zhang
Summary: Coastal erosion has caused increasing damage to coastal shelterbelts. In this study, it was found that inoculation with different AMF strains can improve the growth and nitrogen utilization of Gleditsia sinensis Lam. and Zelkova serrata (Thunb.) Makino under different salt concentrations. Funneliformis mosseae (FM) showed the most significant positive effects on plant growth.
Article
Biodiversity Conservation
Liqin Zhu, Rongzhen Huang, Jinping Wang, Guomin Huang, Hongzhi Guan, Lijing Lin, Mengjia Yang, Yanyan Li, Xianhua Zou
Summary: The study investigates the effects of different organic carbon inputs on soil microbial community structure in a degraded red soil ecosystem. It found that organic carbon inputs significantly changed the species and quantity of soil microorganisms, with a greater impact on fungal community structure than bacterial community structure.
Article
Agronomy
Jin-Ping Wang, Rong-Zhen Huang, Jian-Min Zhao, Ji-Hong Yuan, Guo-Min Huang, Rong-Xiu Xie, Liang Hu, Yue-Fei Xie, G. Geoff Wang
Summary: This study employed Illumina MiSeq sequencing to reveal the effects of cultivars and habitats on the rhizospheric AMF community associated with navel oranges. The results showed that Glomus and Glomus_sp were the dominant genus and species, respectively. Cultivars had a greater impact on AMF diversities compared to habitats, while habitats significantly influenced AMF community compositions. The diversity of AMF was negatively correlated with organic matter content, but positively correlated with available potassium content. Organic matter and available potassium were identified as the most important soil factors affecting the AMF community structure.
ARCHIVES OF AGRONOMY AND SOIL SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Zutan Li, Bingbing Jin, Jingya Fang
Summary: In this study, we propose MetaAc4C, an advanced deep learning model for accurate identification of N4-acetylcytidine (ac4C) sites using pre-trained BERT and various optimization techniques. By adapting generative adversarial networks to address data imbalance and augmenting training RNA samples, our model outperforms existing methods in terms of ACC, MCC, and AUROC.
