Article
Fisheries
Jingxian Sun, Li Liu, Yaoyao Zhan, Jian Song, Weijie Zhang, Bin Wang, Yaqing Chang
Summary: This study provides complete genome descriptions of two Vibrio strains isolated from diseased sea urchins, revealing differences in genetic features, virulence factors, and evolutionary relationships between the causative Vibrio species. The findings enrich current knowledge of the genus Vibrio and highlight the infection efficiency variation and evolutionary diversity of spotting disease causative Vibrio species in sea urchins.
Article
Fisheries
Ruijun Li, Yuxi Huang, Junmin Wu, Huifeng Dang, Luo Wang, Weijie Zhang, Jun Ding
Summary: This study identified a pathogenic strain of Vibrio coralliilyticus, Rb102, from farmed sea urchins with red spotting disease and developed a specific PCR detection method for V. coralliilyticus. Comparative analysis of the transcriptome and metabolome of sea urchins affected by red spotting disease revealed significant differences in the purine metabolic pathway.
AQUACULTURE REPORTS
(2022)
Article
Environmental Sciences
Boqiong Wu, Renhe Jiao, Dongyao Cui, Tanjun Zhao, Jian Song, Yaoyao Zhan, Yaqing Chang
Summary: Comparative miRNA and mRNA transcriptome analyses of sea urchins with different gonadal colors revealed genes and miRNAs associated with sex and gonadal coloration. These findings are important for understanding the process of sea urchin gonad coloration and improving the quality of sea urchins.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Fisheries
Jingxian Sun, Li Liu, Jian Song, Yaoyao Zhan, Weijie Zhang, Bin Wang, Baojing Zhang, Yaqing Chang
Summary: Disease outbreaks are common in cultured sea urchins from China, with two new strains of Vibrio species identified in diseased sea urchins. These strains exhibit different virulence and antimicrobial susceptibility, with one strain showing greater pathogenicity and specific sensitivity to certain antibiotics.
AQUACULTURE REPORTS
(2021)
Article
Fisheries
Panpan Gou, Zhicheng Wang, Jin Yang, Xiuli Wang, Xuemei Qiu
Summary: In this study, the differences in gonad quality between male and female Strongylocentrotus intermedius sea urchins were investigated through transcriptome analysis. A total of 6736 differentially expressed genes (DEGs) were identified, and five DEGs related to gonadal quality were found through enrichment analysis. This study provides fundamental information about the nutrition and taste of S. intermedius gonads.
Article
Environmental Sciences
Lingshu Han, Pengfei Hao, Wenpei Wang, Yanglei Wu, Shuchao Ruan, Chuang Gao, Wanrong Tian, Ye Tian, Xiaonan Li, Luo Wang, Weijie Zhang, Heng Wang, Yaqing Chang, Jun Ding
Summary: In this study, heat-resistant S. intermedius and its control group were used to investigate the molecular mechanisms of heat stress response in sea urchins. Whole-transcriptome RNA sequencing was applied to identify differentially expressed mRNAs, microRNAs, and long noncoding RNAs. A ceRNA regulatory network was constructed, revealing the involvement of ceRNAs in various biological processes. This research provides insight into the potential functions of noncoding RNAs and miRNAs in the heat stress response of S. intermedius.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Genetics & Heredity
Lingshu Han, Zijiao Quan, Yanglei Wu, Pengfei Hao, Wenpei Wang, Yuanxin Li, Xianglei Zhang, Peng Liu, Chuang Gao, Heng Wang, Luo Wang, Weijie Zhang, Donghong Yin, Yaqing Chang, Jun Ding
Summary: This study examines the antioxidant enzyme activities of sea urchins under heat stress and compares their transcriptome and miRNA profiles. The results show that sea urchins' antioxidant enzyme activities are altered by thermal stress, with significant changes observed in the peroxidase activities of red tube-footed sea urchins. The study identifies differentially expressed genes and miRNAs in response to high temperature, suggesting their important functions in the sea urchins' heat response.
FRONTIERS IN GENETICS
(2022)
Article
Fisheries
Fangyuan Hu, Chong Zhao, Peng Ding, Yongchao Li, Ruihuan Tian, Yihai Qiao, Yaqing Chang
Summary: The mass mortality of small sea urchins in summer greatly decreases the production efficiency of longline culture. A newly designed facility was found to significantly decrease morbidity and improve disease resistance and thermal tolerance in cultured sea urchins, suggesting its potential for improving production efficiency at high water temperatures.
Article
Environmental Sciences
Fanshuang Zeng, Luo Wang, Hao Zhen, Chao Guo, Anzheng Liu, Xinglong Xia, Honglin Pei, Changkun Dong, Jun Ding
Summary: Nanoplastics in the ocean have caused severe environmental pollution and worsened the health of aquatic organisms. This study investigated the toxic effects of NPs on marine invertebrates and found that NPs significantly decreased the growth rate and enzyme activity of the organisms, as well as caused atrophy in the intestine and changes in gut bacteria community structure.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biotechnology & Applied Microbiology
Anzheng Liu, Fanshuang Zeng, Luo Wang, Hao Zhen, Xinglong Xia, Honglin Pei, Changkun Dong, Yanmin Zhang, Jun Ding
Summary: In this study, the impact of high temperature on DNA methylation and gene expression in sea urchin was investigated using MethylRAD-seq and RNA-seq. DNA methylation driver genes were identified and it was found that high temperature significantly affected DNA methylation, transcriptome, and growth. The findings suggest that DNA methylation regulates important metabolic processes such as energy metabolism, immune response, and antioxidant functions.
Article
Immunology
Tanjun Zhao, Li Liu, Chengda Li, Yang Zou, Hanyu Yan, Yaoyao Zhan, Yaqing Chang
Summary: This study isolated and characterized the predominant bacteria strains causing black mouth disease in farmed Strongylocentrotus intermedius. The strains were identified as novel Vibrio species and showed dosage-dependent and temperature-dependent pathogenesis in sea urchins. The study also provided insights into the metabolic characteristics and antimicrobial susceptibility of these strains.
MICROBIAL PATHOGENESIS
(2022)
Article
Fisheries
Xiaomei Chi, Xiyuan Huang, Fangyuan Hu, Mingfang Yang, Donghong Yin, Ruihuan Tian, Xiang Li, Yaqing Chang, Chong Zhao
Summary: The present study found that the segregation with two layers of PVC net is an effective approach to preventing the transmission of black mouth disease of S. intermedius. However, the effectiveness of the complete segregation is greatly reduced if the diseased/dead sea urchins are not removed in time. Air exposure deteriorates the transmission of the black mouth disease.
Article
Fisheries
Mingfang Yang, Fangyuan Hu, Xiaofei Leng, Xiaomei Chi, Donghong Yin, Jingyun Ding, Xiang Li, Rantao Zuo, Yaqing Chang, Chong Zhao
Summary: The study found that red light significantly inhibits the growth of Strongylocentrotus intermedius, while sea urchins under blue light have higher righting response time and foraging time.
Article
Fisheries
Li Liu, Yaoyao Zhan, Jingxian Sun, Yingying Li, Wenlu Yin, Weijie Zhang, Yaqing Chang
Summary: The study investigated the effects of morphological traits and body weight on gonadal development in juvenile Strongylocentrotus intermedius using correlation, path, histological, and sex-specific gene expression analyses. Results showed a positive correlation between test diameter, test height, body weight, and gonadal development score, with test diameter being the most important indirect factor affecting gonadal development score. Juvenile Strongylocentrotus intermedius with test diameters greater than 10 mm showed sex differentiation, marking the appearance of reproductive cells and differential expression of sex-specific genes.
Article
Environmental Sciences
Alfabetian Harjuno Condro Haditomo, Masanori Yonezawa, Juanwen Yu, Sayaka Mino, Yuichi Sakai, Tomoo Sawabe
Summary: Sea urchin serves as an indicator of coastal environmental changes and a model organism in developmental biology and evolution. The gut microbiome plays a crucial role in shaping the host's physiology, with significant effects on host growth influenced by diet and rearing environments.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Fisheries
Zhimeng Lv, Guanghui Han, Chenghua Li
Summary: The TIMP1 gene plays a crucial role in inflammatory diseases in vertebrates, responding to bacterial challenge and LPS exposure. AjTIMP1 is expressed in all tissues, with the highest levels in the body wall. Imbalance in AjMMP1/AjTIMP1 ratio was observed in skin ulceration syndrome patients, leading to an inflammatory response with ROS formation and collagen degradation.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Immunology
Zhimeng Lv, Ming Guo, Xuelin Zhao, Yina Shao, Weiwei Zhang, Chenghua Li
Summary: This study provides functional evidence that IL-17 is a conserved cytokine in invertebrates, specifically in the sea cucumber Apostichopus japonicus. The expression of A. japonicus IL-17 (AjIL-17) was positively correlated with the development of inflammation, and the administration of purified recombinant AjIL-17 protein directly promoted inflammation. A novel IL-17R counterpart was identified in the sea cucumber genome and shown to bind AjIL-17. AjIL-17/AjIL-17R regulated inflammatory responses by promoting TRAF6 ubiquitination, p65 nuclear translocation, and coelomocyte proliferation and migration.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Fisheries
Siyuan Zhang, Yina Shao, Dongdong Li, Chenghua Li
Summary: m6A modified lncRNAs in sea cucumbers may serve as potential regulators associated with skin ulceration syndrome (SUS) and provide a promising avenue for disease therapy through targeting METTL3.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Fangyuan Zhou, Lianlian Sun, Yina Shao, Xiumei Zhang, Chenghua Li
Summary: Glutaminolysis plays an important role in the immune response in Apostichopus japonicus. Decreased levels of glutaminolysis metabolic substrates and products were observed, accompanied by an increase in glutathione. The key genes in glutaminolysis were upregulated in response to pathogen infection. AMPK beta can regulate glutaminolysis by influencing the uptake of glutamine. Silencing AjAMPK beta leads to increased levels of glutamine and glutamate, decreased production of glutathione, and a reduction in redox homeostasis indexes.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Junjie Wan, Xuelin Zhao, Jiqing Liu, Kaiyu Chen, Chenghua Li
Summary: In this study, a novel SFK homologue (AjSrc) was identified in Apostichopus japonicus, which mediated Vibrio splendidus-induced coelomocytes' phagocytosis via interacting with focal adhesion kinase (AjFAK) and co-phosphorylation. This study enriched the mechanism of phagocytosis in echinoderm and provided a new theoretical foundation for disease control of sea cucumber.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Cell Biology
Chuili Zeng, Ming Guo, Yangxi Xiang, Mingshan Song, Ke Xiao, Chenghua Li
Summary: This study provides evidence that AjFGF4-AjFGFR2-ERK-cell cycle axis mediated cell proliferation is the driving force for mesentery-derived intestine regeneration in echinoderms.
CELL PROLIFERATION
(2023)
Article
Fisheries
Yuanlan Wu, Xuelin Zhao, Yangxi Xiang, Ming Guo, Chenghua Li
Summary: In this study, the echinoderm MAPK family was identified and analyzed, revealing insights into its evolutionary history and immune function.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Fisheries
Kangrong Yu, Xuelin Zhao, Yangxi Xiang, Chenghua Li
Summary: The hemocytes of invertebrates have different cell subsets with different morphologies and structures, which have different immune functions. In this study, two coelomocyte subpopulations of Apostichopus japonicus were identified and found to have functional differences. Spherical cells showed higher phagocytic capacity and lysosomal activity, suggesting they play a more critical role in immune responses. Transcriptome sequencing analysis revealed significantly different gene expression patterns between the two cell subsets, with spherical cells participating in immune defense and lymphocyte-like cells participating in energy metabolism.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biology
Xianmu Fu, Ming Guo, Jiqing Liu, Chenghua Li
Summary: Circ432, a circRNA identified from sea cucumber with skin ulcer syndrome, has been found to be induced by Vibrio splendidus challenge and LPS exposure. It acts as a sponge for miR-2008 and regulates coelomocytes phagocytosis through the miR-2008-AjELMO1 axis.
COMMUNICATIONS BIOLOGY
(2023)
Article
Microbiology
Yanan Li, Weikang Liang, Chenghua Li
Summary: Vibrio splendidus is an opportunistic pathogen causing aquaculture disease infections globally. The formation of bacterial persister cells contributes to antibiotic treatment difficulties and exogenous adenosine or guanosine can restore susceptibility to tetracycline and effectively kill these persisting cells. Mechanistically, these nucleosides improve intracellular ATP levels, reduce protein aggresomes, and disrupt membrane stability. Furthermore, cells with a small nucleocytoplasmic ratio have higher chances of survival when exposed to tetracycline. Overall, exogenous adenosine or guanosine could be an effective strategy for treating antibiotic-persisting infections.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Microbiology
Fa Dai, Ming Guo, Yina Shao, Chenghua Li
Summary: The study identified a novel T3SS effector protein, STPKLRR, in Vibrio splendidus AJ01. It was found that STPKLRR could phosphorylate AjTmod and promote cytoskeleton rearrangement, leading to self-internalization of the bacteria.
Article
Fisheries
Dongdong Li, Ming Guo, Weikang Liang, Chunhua Jin, Chenghua Li
Summary: This study revealed the functional role of CHOP in sea cucumbers and the mechanism by which it regulates apoptosis. It was found that in sea cucumbers, CHOP mediates apoptosis induced by V. splendidus through the activation of the p38-MAPK pathway rather than the JNK/ERK-MAPK pathway.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Chuili Zeng, Wenjun Tao, Xianmu Fu, Chenghua Li, Ming Guo
Summary: In this study, the researchers investigated the role of circular RNAs (circRNAs) in regulating coelomocyte apoptosis in Apostichopus japonicus. They found that a specific circRNA, circ254, could sponge miR-375 and regulate the expression of AjBAG2, which is involved in coelomocyte apoptosis. Knockdown of circ254 promoted coelomocyte apoptosis, while circ254 silencing down-regulated AjBAG2 expression. These findings suggest that circ254 functions as a competing endogenous RNA (ceRNA) to regulate coelomocyte apoptosis in A. japonicus.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Meixiang You, Lianlian Sun, Chenghua Li, Si Zhu
Summary: Cholesterol metabolism in Apostichopus japonicus is dynamically altered in response to LPS stimulation, with lipophagy playing a crucial role in balancing cholesterol-induced inflammation. This metabolic response is important for regulating various functions including skeletal, muscular, and immune systems.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Review
Cell Biology
Sikou Shen, Yina Shao, Chenghua Li
Summary: Cell death is the irreversible cessation of life and a fundamental physiological process in all organisms, involved in embryonic development, organ maintenance, and autoimmunity. Advances in understanding the mechanisms of cell death have identified different types of programmed cell death and key genes involved. However, little research has focused on the conversion between different cell death modes and their application in diseases. Despite significant discoveries, there are still many unknowns in this field.
CELL DEATH DISCOVERY
(2023)