期刊
FEBS LETTERS
卷 594, 期 3, 页码 519-529出版社
WILEY
DOI: 10.1002/1873-3468.13632
关键词
ceramide; CERT; Chlamydia trachomatis; HeLa cells; inclusion; SGMS1; SGMS2
资金
- JSPS KAKENHI [JP17K07357, JP18K06649]
- MEXT KAKENHI [JP17H06417]
- AMED J-PRIDE [JP19fm0208005j0103]
- AMED-CREST [JP19gm0910005j0005]
The obligate intracellular bacterium Chlamydia trachomatis proliferates in the membranous compartment inclusion formed in host cells. The host ceramide transport protein CERT delivers ceramide from the endoplasmic reticulum to the Golgi complex for the synthesis of sphingomyelin (SM). Chlamydia trachomatis has been suggested to employ CERT to produce SM in the inclusion by host SM synthases (SMSs). Here, we found that C. trachomatis proliferates and produces infective progeny even in SMS1 and SMS2 double-knockout HeLa cells, but not in the SMS1/SMS2/CERT triple-knockout cells. Interestingly, infected cells convert ceramide to SM without host SMSs. These results suggest that C. trachomatis-infected cells can convert ceramide to SM without host SMSs after CERT-mediated transfer of ceramide to the inclusions.
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