Article
Genetics & Heredity
Amalia Namath, Samad Jahandideh, Kate Devine, Caleb B. Kallen, Jeanne E. O'Brien
Summary: The purpose of this study was to assess the rate, rationale, and characteristics of patients who discarded their cryopreserved oocytes, and compare them to patients who continued to preserve their oocytes. The results showed that childbirth was the most common reason for discarding oocytes, and patients who discarded their oocytes had lower AMH levels compared to those who did not.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Article
Reproductive Biology
Maria McClam, Shuo Xiao
Summary: With the significant rise in cancer survival rates, the focus has shifted towards the side effects of treatment and the long-term quality of life for cancer survivors. Ovarian toxicity is a major concern for female cancer patients, and preserving oocytes is crucial for maintaining their reproductive ability. Oncofertility is an emerging field that aims to provide fertility preservation options for cancer patients.
BIOLOGY OF REPRODUCTION
(2022)
Article
Reproductive Biology
Qiaochu Wang, Karla J. Hutt
Summary: Cisplatin can decrease mitochondrial membrane potential in oocytes, leading to cell death. Five weeks after treatment, the number of mature oocytes harvested from cisplatin treated mice was significantly lower, but the surviving oocytes had normal mitochondrial parameters.
JOURNAL OF OVARIAN RESEARCH
(2021)
Article
Immunology
Magdiel Perez-Cruz, Bettina P. Iliopoulou, Katie Hsu, Hsin-Hsu Wu, Tom Erkers, Kavya Swaminathan, Sai-Wen Tang, Cameron S. Bader, Neeraja Kambham, Bryan Xie, Rosemarie H. Dekruyff, Gordon J. Freeman, Everett Meyer
Summary: The study suggests that RGMb may play a role in the pathogenesis of GvHD and IBD. Treatment with an anti-RGMb monoclonal antibody in mouse models prevented GvHD and IBD, while maintaining the graft-versus-tumor effect. The findings highlight the potential of targeting RGMb as a therapeutic strategy for these conditions.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Joon Seok Park, Francesca S. Gazzaniga, Meng Wu, Amalia K. Luthens, Jacob Gillis, Wen Zheng, Martin W. LaFleur, Sarah B. Johnson, Golnaz Morad, Elizabeth M. Park, Yifan Zhou, Stephanie S. Watowich, Jennifer A. Wargo, Gordon J. Freeman, Dennis L. Kasper, Arlene H. Sharpe
Summary: The gut microbiota plays a vital role in regulating anti-tumor immunity during immune checkpoint inhibitor therapy. Certain bacteria have been found to promote an anti-tumor response to immune checkpoint inhibitors in mice, and transplanting fecal samples from responders can improve the efficacy of anti-PD-1 therapy in melanoma patients. However, the effectiveness of fecal transplants varies, and the mechanisms by which gut bacteria promote anti-tumor immunity are still unclear.
Article
Cell Biology
Siu Yu A. Chow, Kazuki Nakayama, Tatsuya Osaki, Maki Sugiyama, Maiko Yamada, Hirotaka Takeuchi, Yoshiho Ikeuchi
Summary: It is discovered that there is more contact between melanocytes and nociceptive neurons in human skin color patch tissue. Co-culture with iPSC-derived sensory neurons promotes morphogenesis and pigmentation of melanocytes. RGMB is identified as a melanocyte-stimulating factor released from sensory neurons. Transcriptome analysis suggests that RGMB can control the melanosome transport machinery of melanocytes.
Article
Multidisciplinary Sciences
Yi Luan, Seok-Yeong Yu, Amirhossein Abazarikia, Rosemary Dong, So-Youn Kim
Summary: Cyclophosphamide and doxorubicin cause premature ovarian insufficiency, and this study clarifies the mechanism behind it. Dormant oocytes are more sensitive to cyclophosphamide, and TAp63 plays a pivotal role in oocyte death induced by cyclophosphamide and doxorubicin, contrary to previous reports.
Article
Multidisciplinary Sciences
Parinaz Kazemi, Teruko Taketo
Summary: The coexistence of metacentric and acrocentric chromosomes in mouse oocytes delays the progression of Meiotic Prophase I and reduces the efficiency of homologous crossover, resulting in a higher frequency of aneuploidy.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Raoul Orvieto, Jacob Farhi, Ravit Nahum, Shani Basch, Jigal Haas, Adva Aizer
Summary: The study found that patients who did not retrieve any oocytes in their first IVF cycle attempt may achieve a 14% cumulative live-birth rate after 5 IVF cycle attempts. Moreover, those who conceived in subsequent IVF cycle attempts were typically those achieving 2 or more fertilized oocytes/TQE.
Article
Developmental Biology
Shawn M. Briley, Avery A. Ahmed, Tessa E. Steenwinkel, Peixin Jiang, Sean M. Hartig, Karen Schindler, Stephanie A. Pangas
Summary: This study demonstrates the importance of UBE2I in oocyte development during folliculogenesis. Knockout of UBE2I in oocytes leads to decreased meiotic competence, abnormalities in spindle architecture and chromosome alignment, and premature arrest in metaphase I. Additionally, UBE2I plays a role in transcriptional regulation in mouse oocytes.
Article
Cell Biology
Shiwen Ni, Teng Zhang, Chenmin Zhou, Min Long, Xuan Hou, Liji You, Hui Li, Lanying Shi, You-Qiang Su
Summary: The formation of IMPDH cytoophidium in mouse ovaries affects oocyte meiosis and development competence, and treatment with IMPDH inhibitor in vitro shows similar effects.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Kyeoung-Hwa Kim, Eun-Young Kim, Kyung-Ah Lee
Summary: The decrease in Gas6 expression in oocytes can lead to adverse effects, but age-related oocyte aging can be improved through GAS6 protein treatment. Restoration of GAS6 can enhance mitochondrial function, reduce oxidative stress levels, increase glutathione levels, and improve the quality of aged oocytes.
Article
Agriculture, Dairy & Animal Science
Ozge Ozmen, Kardelen Karaman
Summary: This study used a bioinformatics approach to investigate the effects of heat stress on different developmental stages of cow oocytes and identified key genes related to heat resistance. The results suggest that different heat response mechanisms may be activated to protect cow oocytes from elevated temperatures.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Endocrinology & Metabolism
Qijing Lei, Eden Zhang, Ans M. M. van Pelt, Geert Hamer
Summary: Although in vitro-derived spermatocytes reached the MI stage and displayed complete chromosome synapsis and normal XY-body formation, meiotic recombination was still insufficient with few crossovers formed, resulting in MI-spermatocytes displaying univalent chromosomes. This indicates the importance of closely monitoring meiotic crossover formation when mimicking gametogenesis in vitro to prevent the generation of aneuploid gametes, as meiotic checkpoints may not function fully in vitro.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Genetics & Heredity
Quynh-Nhu Nguyen, Nadeen Zerafa, Jock K. Findlay, Martha Hickey, Karla J. Hutt
Summary: The research found that mice lacking the apoptosis gene Puma retained 100% of their follicles 24 hours after chemotherapy treatment and showed efficient repair of DNA damage 8 hours post-treatment. Investigation into DNA repair pathways revealed induction of the homologous recombination pathway in damaged cell types, while non-homologous end joining played an insignificant role in primordial follicle oocytes.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Oncology
Thomas Denize, Yue Hou, Jean-Christophe Pignon, Emily Walton, Destiny J. West, Gordon J. Freeman, David A. Braun, Catherine J. Wu, Saurabh Gupta, Robert J. Motzer, Michael B. Atkins, David McDermott, Toni K. Choueiri, Sachet A. Shukla, Sabina Signoretti
Summary: The study explores the molecular correlates of TC PD-L1 expression in ccRCC and its predictive role in anti-PD-1 monotherapy. The results show that TC PD-L1 expression is associated with overexpression of immune-and cell proliferation-related pathways, upregulation of T-cell activation signatures, and increased tumor-infiltrating immune cells. Additionally, a specific molecular RCC subtype enriched in TC PD-L1 positive tumors is found to have longer progression-free survival and higher objective response rate.
CLINICAL CANCER RESEARCH
(2022)
Article
Medicine, Research & Experimental
Sarah C. Cowles, Allison Sheen, Luciano Santollani, Emi A. Lutz, Brianna M. Lax, Joseph R. Palmeri, Gordon J. Freeman, K. Dane Wittrup
Summary: Monoclonal antibodies targeting PD-1 show varied efficacy depending on their affinity, and a threshold affinity is required for maximum efficacy in the treatment of MC38 adenocarcinoma.
Article
Multidisciplinary Sciences
Vijayakumar Velu, Kehmia Titanji, Hasan Ahmed, Ravi Dyavar Shetty, Lakshmi S. Chennareddi, Gordon J. Freeman, Rafi Ahmed, Rama Rao Amara
Summary: The study demonstrated that PD-1 blockade following ART interruption significantly enhances the function of anti-viral CD8 T cells and improves viral control. It suggests the potential synergistic effects of PD-1 blockade with other immunotherapies inducing functional CD8 T-cell responses.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Correction
Immunology
Christopher S. Garris, Sean P. Arlauckas, Rainer H. Kohler, Marcel P. Trefny, Seth Garren, Cecile Piot, Camilla Engblom, Christina Pfirschke, Marie Siwicki, Jeremy Gungabeesoon, Gordon J. Freeman, Sarah E. Warren, SuFey Ong, Erica Browning, Christopher G. Twitty, Robert H. Pierce, Mai H. Le, Alain P. Algazi, Adil I. Daud, Sara I. Pai, Alfred Zippelius, Ralph Weissleder, Mikael J. Pittet
Article
Oncology
Liya Ding, Qiwei Wang, Antons Martincuks, Michael J. Kearns, Tao Jiang, Ziying Lin, Xin Cheng, Changli Qian, Shaozhen Xie, Hye-Jung Kim, Inga-Maria Launonen, Anniina Faerkkilae, Thomas M. Roberts, Gordon J. Freeman, Joyce F. Liu, Panagiotis A. Konstantinopoulos, Ursula Matulonis, Hua Yu, Jean J. Zhao
Summary: This study reveals an adaptive immunosuppression mechanism that leads to resistance to PARP inhibition in BRCA1-mutant ovarian tumors. This resistance is mediated by an increased population of protumor tumor-associated macrophages (TAMs) and activation of the STAT3 signaling pathway. The use of STING agonists can reshape the immunosuppressive tumor microenvironment and overcome PARPi resistance in ovarian cancer.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Chemistry, Multidisciplinary
Min-chun Jiang, Huan-yu Ding, Yu-hong Huang, Chak Kwong Cheng, Chi Wai Lau, Yin Xia, Xiao-qiang Yao, Li Wang, Yu Huang
Summary: Atherosclerotic diseases are a major cause of adult mortality and strain healthcare systems worldwide. A study found that the drug thioridazine can inhibit YAP activity and alleviate endothelial inflammation and atherosclerosis. Thioridazine was shown to have anti-inflammatory effects mediated by inhibiting YAP and regulating RhoA. Furthermore, thioridazine reduced atherosclerosis in two mouse models. This research suggests the potential of repurposing thioridazine for treating atherosclerotic diseases.
ACTA PHARMACOLOGICA SINICA
(2023)
Article
Multidisciplinary Sciences
Heng-Jia Liu, Heng Du, Damir Khabibullin, Mahsa Zarei, Kevin Wei, Gordon J. Freeman, David J. Kwiatkowski, Elizabeth P. Henske
Summary: This study reveals that B7-H3 expression is correlated with immunosuppressive phenotypes and worse clinical outcomes in human tumors with high mTORC1 activity. The authors show that mTORC1 upregulates B7-H3 expression by phosphorylating the transcription factor YY2. Inhibiting B7-H3 enhances T-cell activity and induces tumor cell expression of MHC-II, suppressing tumor growth with mTORC1 hyperactivity.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Jing Liu, Xia Bu, Chen Chu, Xiaoming Dai, John M. Asara, Piotr Sicinski, Gordon J. Freeman, Wenyi Wei
Summary: The protein arginine methyltransferase PRMT1 suppresses the anti-tumor immune response by methylating cGAS and preventing its dimerization. Inhibition of PRMT1 activates the cGAS/STING-dependent DNA sensing signaling and increases the expression of interferon response genes, tumor-infiltrating lymphocytes, and tumoral PD-L1. Combination therapy of PRMT1 inhibitor with anti-PD-1 antibody enhances the anti-tumor therapeutic efficacy.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Enyi Zhu, Yang Liu, Ming Zhong, Yu Liu, Xi Jiang, Xiaorong Shu, Na Li, Hui Guan, Yin Xia, Jinhong Li, Hui-yao Lan, Zhihua Zheng
Summary: The study reveals that neurokinin-1 receptor (NK-1R) promotes renal inflammation and fibrosis in chronic kidney disease (CKD), suggesting that NK-1R could be a potential therapeutic target for CKD patients.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell & Tissue Engineering
Lixia Dong, Huaqi Liao, Linchun Zhao, Jingnan Wang, Congcong Wang, Bowen Wang, Yanqi Sun, Lijun Xu, Yin Xia, Shizhang Ling, Xin Lou, Jinzhong Qin
Summary: The interaction between H3K9 methylation writers and readers is crucial for maintaining the identity of embryonic stem cells.
Article
Biochemistry & Molecular Biology
Lijing Kang, Juanjuan Yi, Chi-Wai Lau, Lei He, Qinghua Chen, Suowen Xu, Jun Li, Yin Xia, Yuanting Zhang, Yu Huang, Li Wang
Summary: Hyperglycemia activates Yes-associated protein 1 (YAP) by suppressing AMPK activity, leading to obesity-associated endothelial dysfunction and inflammation. Metformin improves vascular dysfunction by activating AMPK and promoting YAP phosphorylation.
Article
Medicine, Research & Experimental
Satomi Ando, Charles M. Perkins, Yamato Sajiki, Chase Chastain, Rajesh M. Valanparambil, Andreas Wieland, William H. Hudson, Masao Hashimoto, Suresh S. Ramalingam, Gordon J. Freeman, Rafi Ahmed, Koichi Araki
Summary: T cell exhaustion is associated with dysfunction and expression of PD-1. mTOR inhibition during the expansion phase enhances T cell response, while inhibition after exhaustion progresses causes immunosuppression with decreased TIM3(+) cells and increased viral load. mTOR signaling is essential for differentiation of stem-like T cells into TIM3(+) cells.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Physiology
Yang Liu, Chunhua Xu, Jinhong Li, Yu Zhang, Xiaohua Wang, Yang Wang, Jinzhong Qin, Zhihua Zheng, Yin Xia
Summary: Chronic kidney disease (CKD) is a major health problem characterized by kidney fibrosis. In this study, the researchers investigated the role of the Hippo/yes-associated protein (YAP) pathway in CKD and found that YAP regulates activator protein-1 (AP-1) expression, with Fosl1 being the primary target in renal tubular cells. These findings provide new insights into the underlying mechanisms of CKD and may have implications for potential therapeutic targets.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2023)
Article
Urology & Nephrology
Yu Zhang, Huihui Huang, Yonglun Kong, Chunhua Xu, Liujiang Dai, Xiaoqiang Geng, Yujie Deng, Yang Wang, Yang Liu, Chenling Meng, Xiaoyi Zhang, Jinhong Li, Jinzhong Qin, Bo Feng, Kingston Kinglun Mak, Li Wang, Yu Huang, Weidong Wang, Hui-Yao Lan, Baoxue Yang, Hua A. Jenny Lu, Yin Xia
Summary: Final urine volume and concentration are determined by the regulation of water channels aquaporin (AQP)-2, -3, and -4 in the collecting duct. The Hippo/Yes-associated protein 1 (YAP) pathway and its interaction with various transcription factors play a crucial role in the transcriptional regulation of these AQPs. YAP promotes the transcription of Aqp2 and Aqp4, interacts with GATA2, GATA3, and NFATc1 to control Aqp2 expression, and exploits overlapping mechanisms with Aqp-2, -3, and -4 for their baseline regulation.
KIDNEY INTERNATIONAL
(2023)
