Article
Engineering, Biomedical
Wenhao Cheng, Sundol Kim, Sandra Zivkovic, Hoyong Chung, Yi Ren, Jingjiao Guan
Summary: This study develops a technique for specifically labeling phagosome-derived vesicles in macrophages using microfabricated microparticles. The technique has the potential to advance our understanding of phagocytosis and generate widespread interest in the field of biomaterials science and engineering.
ACTA BIOMATERIALIA
(2022)
Article
Multidisciplinary Sciences
Takayuki Ueno, Yumi Yamamoto, Kiyoshi Kawasaki
Summary: The phagocytosis of polystyrene latex beads by macrophage-like U937 and THP-1 cells enhances pro-inflammatory responses induced by bacterial components, regardless of the uptake mechanism. Additionally, phagocytosis enhances LPS-induced NF-kappa B activation, suggesting that Toll-like receptor (TLR) 4 signaling is enhanced by phagocytosis. Overall, microparticle phagocytosis enhances the innate immune response induced by bacterial components.
SCIENTIFIC REPORTS
(2021)
Article
Engineering, Biomedical
Jiaqi Li, Huangliang Zheng, En-Yu Xu, Michael Moehwald, Linc Chen, Xin Zhang, Shirui Mao
Summary: Polyethylene glycol (PEG) modification is a promising approach to overcome barriers in the deep lung for sustained therapy of pulmonary diseases. PEG-modified microspheres showed enhanced mucus permeability and reduced macrophage uptake, especially the PEG(2000)-DSPE/PLGA 1:1 group. The PEG 2000-based group had significantly prolonged lung retention in vivo and reduced systemic exposure of drugs compared to other groups, making it a promising candidate for sustained lung delivery in pulmonary disease treatment.
ACTA BIOMATERIALIA
(2021)
Article
Materials Science, Biomaterials
Emerson L. Grey, Jazalle McClendon, Joshita Suresh, Scott Alper, William J. Janssen, Stephanie J. Bryant
Summary: Polymeric microparticles formed by a thiol-Michael addition step-growth polymerization reaction were studied for their potential in targeting macrophages. The size of the particles, treatment time, and particle chemistry were found to significantly affect their uptake by macrophages. Amide-terminated particles were non-inflammatory, while carboxyl- and thiol-terminated particles induced inflammation and cytokine production. A lung-specific application of amide-terminated particles was also explored, showing efficient uptake without causing inflammation in vitro and in vivo.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Oncology
Zhilei Cui, Dengfei Xu, Fafu Zhang, Jinyuan Sun, Lin Song, Wenjing Ye, Junxiang Zeng, Min Zhou, Zhengshang Ruan, Linlin Zhang, Rongrong Ren
Summary: The study found that cisplatin (CDDP) increased the expression of CD47 in lung cancer cells, while blocking CD47 enhanced the phagocytic activity of macrophages on CDDP-treated tumor cells. The combination treatment of CDDP and aCD47 exhibited anti-tumor effects and modulated immune cell populations and cytokine levels in the tumor-bearing model.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Environmental Sciences
Jith Thomas, Andrew Stalker, Dalibor Breznan, Errol M. Thomson
Summary: Research shows that different rat strains have varying stress responses to O3 and differences in lung corticosterone levels, which in turn affect the inflammatory gene response of macrophages.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Sylwia Lukasiewicz, Antoni Mikolajczyk, Ewa Blasiak, Ewelina Fic, Marta Dziedzicka-Wasylewska
Summary: The investigation into the interactions between bio-polymeric nanoparticles and a mouse macrophage cell line revealed that non-pegylated PCL NPs were internalized more effectively and utilized the clathrin-mediated pathway. The synthesized NPs show promise as nanocarriers for therapeutic compounds.
Review
Immunology
Christa Decker, Sudeshna Sadhu, Gabrielle Fredman
Summary: The resolution of inflammation is a tissue protective program governed by factors such as specialized pro-resolving mediators. Phagocytes like neutrophils and macrophages play key roles in this process by removing debris, microbes, and dead cells. Enhancing factors and mechanisms associated with phagocytosis and efferocytosis are critical for understanding and treating prevalent human diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Wei Cai, Mengyan Hu, Chunyi Li, Ruizhen Wu, Danli Lu, Chichu Xie, Wei Zhang, Tiemei Li, Shishi Shen, Huipeng Huang, Wei Qiu, Quentin Liu, Yan Lu, Zhengqi Lu
Summary: Proper termination of cell-death-induced neural inflammation is crucial for tissue repair in acute ischemic stroke. FOXP3+ macrophages, a distinct subset of macrophages, display superactive phagocytic capacity and enhance macrophage metabolism through the facilitation of cargo metabolism. FOXP3+ macrophages could be a potential target for immunomodulatory therapy against acute ischemic stroke.
Review
Pharmacology & Pharmacy
Devaraj Ezhilarasan, Karthik Shree Harini
Summary: Kupffer cells (KCs) are resident macrophages of the liver that play a crucial role in the innate immunity. Strategies such as PEGylation, transient clearance of KCs, and surface modification of nanoparticles have shown promise in reducing liver accumulation of nanoparticles. However, the safety of KCs depletion strategies and their impact on the immune system need further investigation.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Wenhao Cheng, Masahiro Fukuda, Sundol Kim, Yuan Liu, Xingchi Chen, Christina Holmes, Yan Li, Hoyong Chung, Yi Ren, Jingjiao Guan
Summary: This study presents a robust engineering method for rupturing phagosomes based on a well-defined mechanism. The method uses microfabricated microparticles composed of uncrosslinked linear poly-(N-isopropylacrylamide) (PNIPAM) as phagocytic objects. By exposing the cells to a cold shock, the microparticle-containing phagosomes rupture. The osmotic pressure generated by dissolved microparticles is the probable cause of phagosomal rupture, as supported by modeling and experimental results.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Pharmacology & Pharmacy
Xinlei Tian, Min Guo, Xiaoya Zhang, Lingfeng Guo, Nan Lan, Yaojun Cheng, Yannan Han, Mingxin Wang, Zhonglu Peng, Changlin Zhou, Hongye Fan
Summary: This study investigated whether Strongylocentrotus nudus eggs polysaccharide (SEP), a non-toxic extract from seafood, contributes to host defense against bacterial infection. The results showed that SEP enhanced bacterial clearance by promoting phagocytosis by macrophages and protected mice from bacterial infection.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Jing-Yang Zhang, Fang -Man Chen, Rong Liu, Jia-Qi Luo, Yong-Cong Huang, Na Shu, Sui-Juan Zheng, Dan Shao, Kam W. Leong, Jin-Zhi Du
Summary: This study identified that the use of the toll-like receptor 7/8 agonist resiquimod (R848) can promote macrophage phagocytosis and reprogram them into an anti-tumoral phenotype. The combination with the anti-SIRP alpha antibody (aSIRP alpha) further enhances the pro-phagocytic effect. A nanoparticle-based delivery system was developed to deliver R848 and aSIRP alpha simultaneously, leading to improved macrophage-mediated cancer immunotherapy.
Review
Biochemistry & Molecular Biology
Maurice B. Hallett
Summary: Phagocytosis is a highly polarised cellular activity where the stimulus and response are focused at one specific part of the cell. The mechanisms for restricting signalling and achieving effective phagocytosis are still not fully understood.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Clinical Neurology
Keun Woo Park, Hyunwoo Ju, Il-Doo Kim, John W. Cave, Yang Guo, Wei Wang, Zhuhao Wu, Sunghee Cho
Summary: The study found that monocyte-derived macrophages (MDMs) play a role in the progressive stroke-induced injury and repair processes. Manipulating monocyte entry at different stroke stages may be an effective immune-based strategy to limit injury propagation in chronic stroke.
Article
Pharmacology & Pharmacy
Xiang Luo, Ling Hu, Huangliang Zheng, Mingqi Liu, Xinrong Liu, Cong Li, Qiujun Qiu, Zitong Zhao, Xiaobo Cheng, Chaoyang Lai, Yuqing Su, Yihui Deng, Yanzhi Song
Article
Oncology
Huangliang Zheng, Jiaqi Li, Xiang Luo, Cong Li, Ling Hu, Qiujun Qiu, Junqiang Ding, Yanzhi Song, Yihui Deng
CANCER CHEMOTHERAPY AND PHARMACOLOGY
(2019)
Article
Pharmacology & Pharmacy
Huangliang Zheng, Jiaqi Li, Mengjing Wang, Xiang Luo, Qiujun Qiu, Ling Hu, Cong Li, Yanzhi Song, Yihui Deng
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2019)
Article
Pharmacology & Pharmacy
Cong Li, Chaoyang Lai, Qiujun Qiu, Xiang Luo, Ling Hu, Huangliang Zheng, Yi Lu, Min Liu, Hongxia Zhang, Xinrong Liu, Yihui Deng, Yanzhi Song
Article
Engineering, Biomedical
Qiujun Qiu, Cong Li, Yanzhi Song, Tao Shi, Xiang Luo, Hongxia Zhang, Ling Hu, Xinyang Yan, Huangliang Zheng, Mengyang Liu, Mingqi Liu, Min Liu, Shuaishuai Yang, Xinrong Liu, Guoliang Chen, Yihui Deng
ACTA BIOMATERIALIA
(2019)
Article
Chemistry, Multidisciplinary
Ling Hu, Xiang Luo, Songlei Zhou, Jingyang Zhu, Mingyue Xiao, Cong Li, Huangliang Zheng, Qiujun Qiu, Chaoyang Lai, Xinrong Liu, Yihui Deng, Yanzhi Song
PHARMACEUTICAL RESEARCH
(2019)
Article
Engineering, Biomedical
Jiaqi Li, Huangliang Zheng, Lu Qin, En-Yu Xu, Linglong Yang, Lan Zhang, Xiaofei Zhang, Linlin Fan, Moritz Beck-Broichsitter, Uwe Muenster, Linc Chen, Yuyang Zhang, Shirui Mao
ACTA BIOMATERIALIA
(2019)
Article
Engineering, Biomedical
Jiaqi Li, Huangliang Zheng, En-Yu Xu, Michael Moehwald, Linc Chen, Xin Zhang, Shirui Mao
Summary: Polyethylene glycol (PEG) modification is a promising approach to overcome barriers in the deep lung for sustained therapy of pulmonary diseases. PEG-modified microspheres showed enhanced mucus permeability and reduced macrophage uptake, especially the PEG(2000)-DSPE/PLGA 1:1 group. The PEG 2000-based group had significantly prolonged lung retention in vivo and reduced systemic exposure of drugs compared to other groups, making it a promising candidate for sustained lung delivery in pulmonary disease treatment.
ACTA BIOMATERIALIA
(2021)
Article
Pharmacology & Pharmacy
Huangliang Zheng, Hai Tao, Jinzhao Wan, Kei Yan Lee, Zhanying Zheng, Sharon Shui Yee Leung
Summary: The performance of the multi-inlet vortex mixer (MIVM) in liposome preparation was investigated, and it was found that the aqueous/organic flow rate ratio and total flow rate have significant effects on liposome size and encapsulation efficiency. Modified MIVMs were able to improve the encapsulation efficiency of lysozyme-loaded liposomes. Therefore, the MIVM shows great potential in liposome preparation.
Review
Chemistry, Multidisciplinary
Jiaqi Li, Huangliang Zheng, Sharon Shui Yee Leung
Summary: Bacterial infections in the respiratory tract pose a major global public health challenge. Pulmonary delivery is an attractive approach for managing these infections, and novel biological antibacterial agents show promising results. This review highlights the causal effects of bacterial infections on pulmonary infectious diseases and provides an overview of emerging antibacterial therapies through nebulization/inhalation of dried powders. Although research on inhaled therapy is still in its infancy, significant progress has been made.
PHARMACEUTICAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Jiaqi Li, Huangliang Zheng, Sharon Shui Yee Leung
Summary: Respiratory Staphylococcus aureus infection is a common problem in lung cancer patients and can invade the tumor progressively and recurrently. The effectiveness of bacteriophages in managing infections during cancer chemotherapy is unknown. This study investigated the interactions between four anticancer drugs and bacteriophage K, and found that Cisplatin reduced phage titers while Gemcitabine and Doxorubicin partially inhibited its propagation. Doxorubicin enhanced the antibacterial capacity of phage K and reduced S. aureus migration. Overall, this work suggests that Doxorubicin has synergistic effects with phage K in combating S. aureus infection and may provide new options for phage clinical transformation and adjunctive application of chemo drugs in infection management.
SCIENTIFIC REPORTS
(2023)