Article
Biotechnology & Applied Microbiology
Shasha Yuan, Sixian Guo, Xihao Huang, Fangang Meng
Summary: This study investigated the interspecies interactions during biofilm development in a moving bed biofilm reactor using regular and time-series phylogenetic molecular ecological networks. The results showed that positive interactions dominated both regular and time-series networks, indicating extensive cooperative behaviors. Time-lagged interspecies interactions prevailed in the biofilm development process, with a higher proportion of directional negative interactions than positive interactions. Keystone species with more positive interactions were positively correlated with biofilm biomass and removal efficiencies, while those targeted negatively by their neighbors showed positive correlations with specific metabolite concentrations.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Food Science & Technology
Jing-Guo Xu, Hui-Xue Hu, Bei-Zhong Han, Jing-Yu Chen
Summary: This study investigated the behavior of Salmonella Enteritidis (SE) in the presence of the environmental bacterium Bacillus paramycoides B5 (Bp5) during dual-species biofilm formation. The results showed that SE initial adhesion was significantly enhanced when cocultured with Bp5. Transcriptome analysis revealed differentially expressed genes (DEGs) in SE during dual-species biofilm formation. Genes involved in chemotaxis, flagellar assembly, and two-component system pathways were upregulated, while genes involved in amino acid biosynthesis and metabolism were downregulated. This study provides insights into the enhanced biofilm formation and environmental resistance of SE in dual-species biofilms.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Masoumeh Moradi, Yi Yang, Dake Xu, Zhenlun Song, Fuhui Wang
Summary: The study found that the presence of marine Vibrio azureus and Jeotgalibacillus alkaliphilus together significantly enhances the growth rate and biomass of biofilm, with V. azureus showing dominance in competition. Electrochemical results showed increased corrosion rates of duplex stainless steel in presence of bacterial mixture.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2021)
Article
Multidisciplinary Sciences
Robert Zarnowski, Hiram Sanchez, Anna Jaromin, Urszula J. Zarnowska, Jeniel E. Nett, Aaron P. Mitchell, David Andes
Summary: Extracellular vesicles play an important role in mediating community interactions among cells. In this study, researchers investigated the composition and function of biofilm-associated vesicles in different Candida species. They found similarities in vesicle size and release over the biofilm lifespan, and identified a group of proteins enriched in these vesicles that are important for biofilm processes. By delivering wild-type vesicle cargo, they were able to restore mutant phenotypes and observed functional complementation in cross-species biofilm interactions. These findings highlight the significance of vesicles in shaping protective biofilm communities.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Engineering, Chemical
Anh Q. Nguyen, Luong N. Nguyen, Zhicheng Xu, Wenhai Luo, Long D. Nghiem
Summary: This study investigated fouling-associated microbial communities in a laboratory-scale MBR at different fouling stages, revealing differences in microbial structure between mixed liquor and biofilm, with specific taxa like Victivallales and Blastocatellia 11-24 playing key roles in fouling propensity. Despite their low abundances, these key players positively correlated with fouling indicators, contributing to the development of new fouling control strategies. The biofilm community showed a more complex structure and higher inter-species interaction compared to the mixed liquor community, indicating greater stability and synergy among microbial taxa in the biofilm.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Article
Orthopedics
Ida Gitajn, Paul Werth, Robert O'Toole, Mandarin Joshi, David Jevsevar, Brent Wise, Ajinya Rane, Steven Horton, Emily A. McClure, Benjamin Ross, Carey Nadell
Summary: This study found that common microbial taxa, such as Enterobacter, Enterococcus, and Pseudomonas, tend to co-occur with other microbes in fracture-related infections over 75% of the time. These co-occurring microbes have demonstrated interactive relationships in other diseases, suggesting that similar important interactions may exist in fracture-related infections. Understanding and potentially disrupting these intermicrobial relationships can improve the treatment of established infections and prevent infection in high-risk patients.
JOURNAL OF ORTHOPAEDIC TRAUMA
(2022)
Article
Chemistry, Multidisciplinary
Rasmus Munter, Camilla Stavnsbjerg, Esben Christensen, Mikkel E. Thomsen, Allan Stensballe, Anders E. Hansen, Ladan Parhamifar, Kasper Kristensen, Jens B. Simonsen, Jannik B. Larsen, Thomas L. Andresen
Summary: Coating nanoparticles with PEG does not effectively make the particles stealth to surface active proteins. A microscopy-based method is introduced to directly observe opsonization at the single nanoparticle level. The study reveals that different surface coatings have significant effects on opsonization on liposomes and that opsonization is heterogeneous, with both opsonized and non-opsonized liposomes co-existing. This assay provides mechanistic details about opsonization at the single nanoparticle level previously inaccessible to established bulk assays.
Article
Multidisciplinary Sciences
Charlotte Carrier-Belleau, David Drolet, Christopher W. McKindsey, Philippe Archambault
Summary: The increasing number and diversity of anthropogenic stressors in marine habitats have negative impacts on biological systems, biodiversity, and ecosystem functions. Methods to assess cumulative effects include experimental manipulations, which may identify non-linear responses. Salinity variation and nutrient enrichment had strong impacts on a bivalve assemblage, with effects varying through time and showing antagonistic interactions.
SCIENTIFIC REPORTS
(2021)
Article
Engineering, Manufacturing
Xing Zhang, Wenye Ye, Leslie Mushongera, Yiliang Liao
Summary: This study investigated the heterogeneities of a Ti-35Nb alloy during laser powder bed fusion (LPBF) process and developed a computational framework to understand the process-microstructure-property relationship. The experimental results showed significant changes in the heterogeneities across the melt pool under LPBF conditions. The spatial distribution maps of cell size and microhardness demonstrated the heterogeneities within the melt pool. The effects of process-induced thermal conditions on sub-grain cellular structure and micromechanical response were evaluated.
ADDITIVE MANUFACTURING
(2022)
Review
Microbiology
Susana Patricia Lopes, Paula Jorge, Ana Margarida Sousa, Maria Olivia Pereira
Summary: Antimicrobial therapy is facing a worrying challenge known as heteroresistance (HR), which has been gradually documented in clinically relevant pathogens. This phenomenon complicates chronic infections mediated by polymicrobial biofilms, with organisms displaying diverse physiological states and metabolic pathways. The review clarifies terminologies related to resistance and discusses recent HR incidences, potential mechanisms, and the link between HR and biofilms, providing critical insights into the clinical implications of HR in antimicrobial resistance spreading.
CRITICAL REVIEWS IN MICROBIOLOGY
(2021)
Article
Biology
Diana P. Lopez, Amy L. Freestone
Summary: Biotic interactions in assembly processes vary across time and latitude, affecting trait assembly in marine assemblages. Competition is stronger in later stages and predation is more intense at lower latitudes. Different regions show different patterns of trait assembly, with trait divergence occurring in late assembly at higher latitudes and relaxed predation causing trait divergence in tropical regions.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Article
Microbiology
Xiaozhen Liu, Qinmeng Liu, Sihuai Sun, Hengxi Sun, Yao Wang, Xihui Shen, Lei Zhang
Summary: This study identified genes related to quorum sensing (QS) in rumen microbial genomes and found that these genes were prevalent and highly expressed in the rumen, suggesting a universal cell-to-cell communication and social behavior mediated by QS in rumen microbes.
Article
Engineering, Environmental
Ishaan Markale, Maxence Carrel, Dorothee L. Kurz, VeronicaL. Morales, Markus Holzner, Joaquin Jimenez-Martinez
Summary: Internal heterogeneity of biofilms plays a crucial role in enhancing fluid mixing and reactions within porous media, which has significant implications for industrial and environmental applications. This study utilizes advanced imaging techniques to investigate the impact of internal heterogeneity on fluid flow and solute transport in bacterial biofilms. The results show that internal heterogeneity mainly affects intermediate velocities and significantly influences biologically driven reactions. Understanding and considering the internal heterogeneity of biofilms is important for predicting reactivity in bioclogged porous systems.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Microbiology
Eva M. Voglauer, Benjamin Zwirzitz, Sarah Thalguter, Evelyne Selberherr, Martin Wagner, Kathrin Rychli
Summary: This study reveals the presence of biofilms in water hoses in the food processing environment, which are mainly dominated by Proteobacteria, represented mainly by Comamonadaceae and Pseudoxanthomonas. The microbial community of biofilms is less diverse than the water microbial community, and those from different sample sites are distinct from each other.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Diego A. Esquivel-Hernandez, Jonathan S. Garcia-Perez, Xiaoyin Xu, Sanya Metha, Juan Maldonado, Siqing Xia, He-Ping Zhao, Bruce E. Rittmann, Aura Ontiveros-Valencia
Summary: The reduction of selenate in hydrogen-fed membrane biofilm reactors was studied in combination with other common electron acceptors. Results showed that the microbial communities had a high diversity and resilience in terms of selenate and nitrate reductions, influenced by the rare biosphere. Changes in electron acceptor or donor loading altered the phylogenetic structure of the communities, but biofilm communities were resilient.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Ecology
Wenzheng Liu, Jakob Russel, Mette Burmolle, Soren J. Sorensen, Jonas S. Madsen
Review
Food Science & Technology
Lei Yuan, Mads Frederik Hansen, Henriette Lyng Roder, Ni Wang, Mette Burmolle, Guoqing He
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2020)
Article
Environmental Sciences
Henriette Lyng Roder, Wenzheng Liu, Soren Johannes Sorensen, Jonas Stenlokke Madsen, Mette Burmolle
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2019)
Article
Ecology
Demeng Tan, Mads Frederik Hansen, Luis Nunes de Carvalho, Henriette Lyng Roder, Mette Burmolle, Mathias Middelboe, Sine Lo Svenningsen
Article
Biotechnology & Applied Microbiology
Urvish Trivedi, Cody Fell, Jonas S. Madsen, Jake Everett, Mette Burmolle, Kendra P. Rumbaugh, Soren J. Sorensen
NPJ BIOFILMS AND MICROBIOMES
(2020)
Review
Microbiology
Faizan Ahmed Sadiq, Mette Burmolle, Marc Heyndrickx, Steve Flint, Wenwei Lu, Wei Chen, Jianxin Zhao, Hao Zhang
Summary: Most bacterial species exist in the form of surface-adhered communities or biofilms in various environments. Researchers are now focusing on studying multispecies biofilms and revealing interspecific interactions due to the coexistence of numerous bacterial species. Emergent properties in multispecies biofilms are triggered by bacterial social interactions, which are important for controlling and manipulating microbial communities.
CRITICAL REVIEWS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Henriette Lyng Roder, Urvish Trivedi, Jakob Russel, Kasper Norskov Kragh, Jakob Herschend, Ida Thalso-Madsen, Tim Tolker-Nielsen, Thomas Bjarnsholt, Mette Burmolle, Jonas Stenlokke Madsen
Summary: Plasmids can be stored in biofilms, allowing them to persist even under non-selective conditions. However, under these conditions, cells carrying plasmids may promote the dispersal of cells without plasmids, turning biofilms into plasmid sinks.
NPJ BIOFILMS AND MICROBIOMES
(2021)
Article
Biotechnology & Applied Microbiology
Nan Yang, Joseph Nesme, Henriette Lyng Roder, Xuanji Li, Zhangli Zuo, Morten Petersen, Mette Burmolle, Soren Johannes Sorensen
Summary: The study found that introducing a symbiotic bacterial consortium SPMX comprising four bacterial species can significantly enhance drought tolerance in Arabidopsis plants. Additionally, the addition of SPMX helps stabilize the diversity and structure of root-associated microbiomes, contributing to overall plant health under drought stress. These SPMX-induced changes collectively result in increased drought tolerance in plants, highlighting the potential for engineering emergent bacterial community properties to improve plant resilience to drought.
NPJ BIOFILMS AND MICROBIOMES
(2021)
Review
Microbiology
Karin Sauer, Paul Stoodley, Darla M. Goeres, Luanne Hall-Stoodley, Mette Burmolle, Philip S. Stewart, Thomas Bjarnsholt
Summary: This review introduces a model of bacterial biofilm formation and points out that the current five-step model fails to capture many aspects of biofilm physiology. The authors propose a more inclusive model that can accommodate various scenarios of biofilm formation.
NATURE REVIEWS MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Nathalie N. S. E. Henriksen, Mads Frederik Hansen, Heiko T. Kiesewalter, Jakob Russel, Joseph Nesme, Kevin R. Foster, Birte Svensson, Gunnar Oregaard, Jakob Herschend, Mette Burmolle
Summary: This study shows that cultivation in a structured environment facilitates coexistence, evolution, and adaptation of bacterial communities. Co-cultivation in a biofilm model resulted in stable coexistence of two bacterial species and drove the evolution of high-yield variants. This study emphasizes the importance of structured environments for the cultivation and evolution of industrially important bacterial communities.
NPJ BIOFILMS AND MICROBIOMES
(2022)
Article
Microbiology
Jiapeng Hou, Lutian Wang, Martin Alm, Peter Thomsen, Tor Monsen, Madeleine Ramstedt, Mette Burmolle
Summary: Catheter-associated urinary tract infections (CAUTI) are a common clinical concern, especially in long-term catheterized patients. Previous strategies to tackle CAUTI mainly focused on mono- or dual-species biofilms, which do not accurately represent the complexity of long-term CAUTI cases involving multiple species. In this study, a four-species biofilm model on catheters was developed to evaluate the interactions and responses to antibiotics. The results showed complex interactions between species, which affected their individual abundances and susceptibility to antibiotics. This highlights the importance of considering the multispecies nature of CAUTI in treatment and control strategies.
Review
Infectious Diseases
Andreia R. R. Tome, Fabio M. Carvalho, Rita Teixeira-Santos, Mette Burmolle, Filipe J. M. Mergulhao, Luciana C. C. Gomes
Summary: This review discusses the effects of probiotics and their metabolites on pre-established biofilms in the food industry. It shows that probiotics can effectively disrupt biofilms formed by various foodborne microorganisms, making it a promising approach.
Article
Biotechnology & Applied Microbiology
Henriette Lyng Roder, Eleni Christidi, Cristina I. Amador, Samra Music, Asmus Kalckar Olesen, Birte Svensson, Jonas Stenlokke Madsen, Jakob Herschend, Jan-Ulrich Kreft, Mette Burmolle
Summary: The study revealed that flagella play a significant role in impacting plasmid transfer and uptake in bacterial biofilms, with mechanisms such as steric hindrance of pilus movement and binding reducing the frequency of contact and plasmid uptake. This finding adds a new layer of complexity to understanding bacterial conjugation in biofilms beyond traditional factors like distance and lag times.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Ecology
Faizan Ahmed Sadiq, Koen De Reu, Hans Steenackers, Ann van de Walle, Mette Burmolle, Marc Heyndrickx
Summary: This study investigated interspecies interactions in mixed-species biofilms and revealed dynamic social interactions among bacteria, including commensalism, exploitation, and amensalism. The keystone species M. lacticum was found to enhance the growth of other species, leading to synergy in biofilm mass. The findings can be applied to cultivate, engineer, and design synthetic bacterial communities based on interaction-mediated approaches.
ISME COMMUNICATIONS
(2023)