Article
Chemistry, Analytical
Brandon Hayes, Lawrence Smith, Heiko Kabutz, Austin C. Hayes, Gregory L. Whiting, Kaushik Jayaram, Robert MacCurdy
Summary: Thermal bubble-driven micro-pumps are actuation technology that can displace fluid without any moving parts. This study presents a low-cost approach using commercial substrates and tooling to accelerate the research and development of thermal bubble-driven micro-pumps.
Review
Engineering, Multidisciplinary
Dianming Li, Guichu Yue, Shuai Li, Jing Liu, Huaike Li, Yuan Gao, Jingchong Liu, Lanlan Hou, Xiaofeng Liu, Zhimin Cui, Nu Wang, Jie Bai, Yong Zhao
Summary: This review focuses on the preparation of multi-structure fibrous nanomaterials using multi-fluidic electrospinning technique and discusses their applications in energy, catalysis, and biology. The working principle and process of electrospinning are introduced, followed by the combination of microfluidic concept with electrospinning technique to achieve multi-fluidic electrospinning. The applications of multi-structure nanofibers in energy (Li'/Na' batteries and Li-S batteries), hetero-catalysis, and biology (drug delivery and tissue engineering) are then presented. Finally, the challenges and future directions in this emerging field are summarized.
Article
Nuclear Science & Technology
Jing-Gang Li, Jing-Han Peng, Chao Wang, Jun Chen, Fei Xu, Yun-Fan Ma
Summary: This study investigates the impact of different gadolinium rod design schemes on core axial power distribution and evaluates their effect on main core characteristics. The results show that the new gadolinium rod design significantly improves the core axial power distribution, enhancing reactor operation stability and core safety margin.
NUCLEAR SCIENCE AND TECHNIQUES
(2022)
Article
Chemistry, Analytical
Sadeq Abu-Dawas, Hawra Alawami, Mohammed Zourob, Qasem Ramadan
Summary: A low-cost, versatile, and reconfigurable fluidic routing system and chip assembly have been developed, showcasing the custom design of chip-to-chip communication and versatility for various biological models.
Article
Chemistry, Multidisciplinary
Yao Chen, Chen Ni, Lin Jiang, Zhonghua Ni, Nan Xiang
Summary: An inertial multi-force deformability cytometry (IMFDC) is developed for high-throughput, high-accuracy, and high-applicability tumor cell mechanotyping. The IMFDC is able to evaluate the deformability of different cells and identify cell types. The multiple-parameters-based machine learning model achieves high discrimination accuracy and throughput. The IMFDC has important applications in clinical cancer diagnosis.
Review
Chemistry, Analytical
Ajeet Singh Yadav, Tran Du Tuan, Adrian J. T. Teo, Yuchen Dai, Fariba Malekpour Galogahi, Chin Hong Ooi, Nguyen Nam-Trung
Summary: Core-shell particles are micro- or nanoparticles with solid, liquid, or gas cores encapsulated by protective solid shells, which have attracted increasing attention as versatile carriers for pharmaceutical and biomedical applications. This review provides an overview of fabrication methods, and discusses their application and manipulation, including assembly, sorting, and triggered release. Approaches for diagnostics and drug delivery are also discussed in detail.
Article
Chemistry, Analytical
Lei Wei, Guoqing Fang, Zhongwen Kuang, Lin Cheng, Huaping Wu, Daoyou Guo, Aiping Liu
Summary: This study introduces an accessible, low-cost fabrication and integration scheme for flexible microfluidic architecture capable of reliable sweat collection and sensing. It utilizes low-cost 3D printing technology to create positive molds from easy-to-print materials for personalized customization of microfluidic channels. An innovative approach is proposed to encapsulate microfluidic channels and integrate pH sweat sensors, providing a cost-effective alternative to soft-lithography methods and reducing the threshold of microfluidic development and manufacture.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Kaixin Sun, Fan Xu, Jingji Liu, Yajun Zhang, Yiqiang Fan
Summary: This study proposed a method for rapidly fabricating paper-based microfluidic devices and demonstrated its application in quantitatively analyzing amino acid content in tea. The method offers advantages of fast fabrication and low cost, and shows great potential for various applications.
Article
Multidisciplinary Sciences
Yuanyuan Liao, Yves Mechulam, Benedikt Lassalle-Kaiser
Summary: This study presents a novel microfluidic mixer based on the staggered herring bone pattern and split-recombination strategies, which improves mixing efficiency and reduces sample consumption. The optimized geometry allows for controlled flow structure rotation and increased contact surface, leading to enhanced mixing effects. The device, fabricated using soft-lithography, demonstrates good mixing performance under low flow rate and small working volume conditions, making it suitable for studies with precious samples.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Alexander Kutscher, Paula Kalenczuk, Mohammed Shahadha, Stefan Gruenzner, Franziska Obst, Denise Gruner, Georgi Paschew, Anthony Beck, Steffen Howitz, Andreas Richter
Summary: In this study, an alternative manufacturing process based on multi-material 3D printing is proposed to efficiently print various active polymers as microvalves on large-area substrates. The printing procedures, influencing variables, and special features are discussed using two different examples of chemofluidic valves. The functionality of the concept is demonstrated in a specific chemofluidic chip for automating analysis procedures, showing that multi-material 3D printing offers faster and more flexible production of active-material devices with comparable tolerances to photolithography, particularly for small quantities.
Article
Engineering, Multidisciplinary
Diego A. Aligia, Bruno A. Roccia, Cristian H. De Angelo, Guillermo A. Magallan, Guillermo N. Gonzalez
Summary: A new orientation estimation strategy for a non-accelerated platform is proposed in this work, using a low-cost IMU and addressing common offset issues on the magnetometer with a recursive least-square algorithm. Implemented with a TM4C123GXL microcontroller and a MPU9150 sensor, the strategy is proven to be feasible through experimental results for calibration and estimation procedures.
Article
Chemistry, Analytical
Mohammad Derakhshani, Amir Jahanshahi, Hedayatollah Ghourchian
Summary: This manuscript proposes a new device, the Volume-Independent Autonomous box (VIA box), which addresses the challenges of the colorimetric method in microfluidic paper-based and microfluidic thread/paper-based analytical devices. The VIA box continuously monitors the transient color of the device and interprets the color profile over time to measure the glucose level independent of sample volume. Unlike previous studies, the VIA box uses an ordinary RGB sensor instead of an imaging camera, resulting in low computational cost.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Shaofei Shen, Xin Wang, Yanbing Niu
Summary: Inertial microfluidics technology has been utilized to manipulate fluid and particles for biomedical and clinical applications. By introducing ordered micro-obstacles in a semicircular microchannel with ultra-low aspect ratio, this study achieved improved fluid mixing effects and manipulation of fluid and particles. The research provides insights into the geometry-induced multi-vortex mechanism, which can potentially simplify biochemical and clinical operations.
Article
Biochemical Research Methods
Cailian Wang, Yan Chen, Xuyu Gu, Xiuxiu Zhang, Chanchan Gao, Lijun Dong, Shiya Zheng, Shicheng Feng, Nan Xiang
Summary: We developed a low-cost polymer-film spiral inertial microfluidic device for the effective size-dependent separation of malignant tumor cells. The device successfully separated tumor cells from white blood cells and rare cells, with high recovery rates and removal rates. It was also successfully employed for processing clinical samples, improving the enrichment of malignant tumor cells and downstream diagnostic accuracy. The device offers the advantages of label-free operation, low cost, and fast fabrication, making it a potential tool for effective cell separation.
Article
Nanoscience & Nanotechnology
Mohammad Amin Raoufi, Hossein Ahmadi Nejad Joushani, Sajad Razavi Bazaz, Lin Ding, Mohsen Asadnia, Majid Ebrahimi Warkiani
Summary: This study thoroughly investigates the effects of solution rheology and Dean drag force on elasto-inertial focusing of particles and cells within a spiral microchannel. It demonstrates the potential for position-adjustable and size-tunable particle and cell focusing phenomenon, while also evaluating cell viability, morphology, and growth rate after focusing. The research provides better understanding of focusing phenomena in viscoelastic fluids within spiral microfluidic channels.
MICROFLUIDICS AND NANOFLUIDICS
(2021)
Article
Biochemistry & Molecular Biology
Nan Xiang, Yihan Zhao, Bing Zhang, Qiuming Gu, Weiling Chen, Xinbo Guo
Summary: In this study, the volatile profiles during young pomelo fruits development were investigated, and the prominent volatile components were identified. The regulatory roles of transcription factors on volatiles accumulation in young pomelos were also explored. The research findings contribute to the extraction of profitable volatiles and enrich the comprehensive utilization of young pomelos.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Chemistry, Analytical
Yao Chen, Zheng Zhou, Shu Zhu, Zhonghua Ni, Nan Xiang
Summary: The rapid development of microfluidic technologies has provided a promising tool for single-cell analysis, enabling a deeper understanding of the physiological and pathological states of cells. This review provides a comprehensive overview of the recent advances in label-free microfluidics for analyzing the biophysical properties of single cells, and discusses their applications in cell identification, disease diagnosis, and drug resistance studies.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Dezhi Tang, Lin Jiang, Wenlai Tang, Nan Xiang, Zhonghua Ni
Summary: This paper proposes a cost-effective portable microfluidic impedance cytometer (MIC) for broadband impedance analysis of cells using maximum length sequence (MLS) and viscoelastic focusing. Results show that MIC has the potential for rapid detection of circulating tumor cells.
Article
Agriculture, Multidisciplinary
Bing Zhang, Tianxiang Wen, Nan Xiang, Yihan Zhao, Xinbo Guo
Summary: Ultrasonic pretreatment was found to increase the accumulation of tocopherols and carotenoids in maize seedlings but decrease tocotrienol synthesis. The carotenoid content reached its highest level at 8 minutes of ultrasonic pretreatment, while tocopherol initially dropped but began to rise after 8 minutes. The predominant component of tocochromanols in maize seedlings shifted from gamma-tocotrienol to alpha-tocopherol after ultrasonic pretreatment.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Review
Chemistry, Analytical
Lin Jiang, Hang Yang, Weiqi Cheng, Zhonghua Ni, Nan Xiang
Summary: Circulating tumor cells (CTCs) are important biomarkers in liquid biopsy, and their number and heterogeneity play a crucial role in cancer diagnosis and personalized medicine. However, due to their low abundance, conventional methods can only detect CTCs at the population level. Therefore, droplet microfluidics, as a high-throughput and sensitive single-cell analysis platform, becomes a significant tool for quantitative detection and heterogeneity analysis of CTCs.
Article
Biochemical Research Methods
Hang Yang, Lin Jiang, Kefan Guo, Nan Xiang
Summary: We developed a manually operated device based on a static droplet array (SDA) for the synthesis of nonspherical microparticles. The device utilized an improved SDA structure and reversible bonding between PDMS for large-scale synthesis and rapid extraction. We investigated the effects of flow rate, SDA well size, and shape on droplet generation and found that it was insensitive to flow rate. The device was capable of producing monodisperse droplets by manual pushing of the syringe. Additionally, we successfully integrated four different SDA structures in one device and achieved synthesis and extraction of nonspherical microparticles with various shapes and materials.
Review
Chemistry, Analytical
Yao Chen, Kefan Guo, Lin Jiang, Shu Zhu, Zhonghua Ni, Nan Xiang
Summary: Cell deformability plays a crucial role in cellular processes and functions, and its measurement at the single-cell level is highly sought after in biomedicine for various applications. Microfluidics has emerged as a promising tool for single-cell deformability analysis, offering advantages such as reduced complexity and reagent consumption. This review discusses recent advances in microfluidic technologies for single-cell deformability analysis, including different methods of stress generation and their respective applications, advantages, and disadvantages. Future research directions are also explored, such as improving detection performance and integration of detection technologies.
Article
Chemistry, Analytical
Nan Xiang, Zhonghua Ni
Review
Biochemical Research Methods
Shu Zhu, Yaohui Fang, Kefan Guo, Zhonghua Ni, Nan Xiang
Summary: Conventional cancer diagnosis requires invasive tissue biopsy, causing severe harm to patients. Liquid biopsy, with its minimal invasiveness, has shown potential for real-time cancer diagnosis but still cannot replace tissue biopsy in most settings. This paper discusses the challenges and limitations of existing liquid biopsy instruments, as well as the opportunities and future progression of next-generation tools. Ultimately, the goal is to integrate liquid biopsy into clinical workflows and establish it as a validated and reliable tool for cancer diagnosis.
Article
Materials Science, Multidisciplinary
Nan Xiang, Lin Li, Hua Wang, Jiang Qian, Tonghua Wang
Summary: Graphene oxide-polyvinyl alcohol mixed matrix membranes were prepared on the surface of polysulfone membranes for the dehydration of alcohols through pervaporation. The concentration and size of graphene oxide nanosheets were found to affect the separation performance of the membranes.
JOURNAL OF MATERIALS SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Yao Chen, Chen Ni, Lin Jiang, Zhonghua Ni, Nan Xiang
Summary: An inertial multi-force deformability cytometry (IMFDC) is developed for high-throughput, high-accuracy, and high-applicability tumor cell mechanotyping. The IMFDC is able to evaluate the deformability of different cells and identify cell types. The multiple-parameters-based machine learning model achieves high discrimination accuracy and throughput. The IMFDC has important applications in clinical cancer diagnosis.
Article
Food Science & Technology
Yaoyao Cheng, Nan Xiang, Honglin Chen, Yihan Zhao, Lixia Wang, Xuzhen Cheng, Xinbo Guo
Summary: This study aimed to investigate the regulatory mechanisms of white, blue, and red lights on carotenoid and tocopherol biosynthesis in mung bean sprouts. Results showed that these lights stimulated the increase of carotenoids and tocopherols in sprouts, with the best yield observed under white light. Genes involved in carotenoid and tocopherol biosynthesis exhibited high expression levels under light quality conditions. These findings provide insights into light-mediated regulation and fortification of carotenoids and tocopherols, as well as guidance for future agricultural cultivation of mung bean sprouts.
FOOD CHEMISTRY: MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Yao Chen, Lin Jiang, Xiaozhe Zhang, Zhonghua Ni, Nan Xiang
Summary: This article introduces an efficient label-free sorting and mechanical phenotyping method for tumor cells, which utilizes a viscoelastic-sorting integrated deformability cytometer (VSDC) to sort, extract, and analyze the mechanical properties of tumor cells. Machine learning algorithms are used to distinguish tumor cells from white blood cells.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Zheng Zhou, Chen Ni, Zhixian Zhu, Yao Chen, Zhonghua Ni, Nan Xiang
Summary: Cell mechanical properties can be used as label-free markers to indicate cell states and disease processes. This study proposes a high-throughput adjustable deformability cytometry system that combines three-dimensional elasto-inertial focusing and a virtual fluidic channel to enable fast mechanical phenotyping of various cell sizes.
Article
Biochemical Research Methods
Nan Xiang, Zhonghua Ni
Summary: Inertial microfluidics is a technique that utilizes hydrodynamic effects induced at finite Reynolds numbers to passively manipulate particles, cells, or fluids. It offers advantages such as high-throughput processing, simple channel geometry, and label-free and external field-free operation. It has gained increasing interest and is widely used in single-cell detection and analysis for sample preparation. Although there have been achievements in the field, improvements can still be made in the performance and outcomes of inertial microfluidics with a better understanding of the physical mechanisms and the development of novel channels, integration strategies, and commercial instruments. Enhanced inertial microfluidic platforms may be a new foundation for advancing biomedical research and disease diagnosis.