Nucleic acids delivery methods for genome editing in zygotes and embryos: the old, the new, and the old-new

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

(2015) Sergey Shmakov et al.   MOLECULAR CELL

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Genome editing at the crossroads of delivery, specificity, and fidelity

(2015) Ignazio Maggio et al.   TRENDS IN BIOTECHNOLOGY

GONAD: Genome-editing via Oviductal Nucleic Acids Delivery system: a novel microinjection independent genome engineering method in mice

(2015) Gou Takahashi et al.   Scientific Reports

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing

(2015) Masakazu Hashimoto et al.   Scientific Reports

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

A CRISPR view of development

(2014) Melissa M. Harrison et al.   GENES & DEVELOPMENT

Adenoviral vector DNA for accurate genome editing with engineered nucleases

(2014) Maarten Holkers et al.   NATURE METHODS

Microinjection free delivery of miRNA inhibitor into zygotes

(2014) Jin Young Joo et al.   Scientific Reports

Simple knockout by electroporation of engineered endonucleases into intact rat embryos

(2014) Takehito Kaneko et al.   Scientific Reports

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

Correction: Release of ATP from Marginal Cells in the Cochlea of Neonatal Rats Can Be Induced by Changes in Extracellular and Intracellular Ion Concentrations

(2012) Yating Peng et al.   PLoS One

In vivogene transfer in mouse preimplantation embryos after intraoviductal injection of plasmid DNA and subsequentin vivoelectroporation

(2012) Masahiro Sato et al.   Systems Biology in Reproductive Medicine

Targeting DNA With Fingers and TALENs

(2012) Daniel F Carlson et al.   Molecular Therapy-Nucleic Acids

Lentiviral Mediated Transgenesis by In Vivo Manipulation of Spermatogonial Stem Cells

(2011) Lalit Sehgal et al.   PLoS One

The creation of transgenic pigs expressing human proteins using BAC-derived, full-length genes and intracytoplasmic sperm injection-mediated gene transfer

(2011) Masahito Watanabe et al.   TRANSGENIC RESEARCH

Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression

(2010) Masato Ohtsuka et al.   NUCLEIC ACIDS RESEARCH

In-vivo gene transfer induces transgene expression in cells and secretions of the mouse cauda epididymis

(2009) P. Esponda et al.   MOLECULAR HUMAN REPRODUCTION

Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases

(2009) Aron M. Geurts et al.   SCIENCE

Zonula occludens-1 (ZO-1) is involved in morula to blastocyst transformation in the mouse

(2008) Hehai Wang et al.   DEVELOPMENTAL BIOLOGY

Progress in gene transfer by germ cells in mammals

(2008) Yidong Niu et al.   Journal of Genetics and Genomics

Transgenesis via permanent integration of genes in repopulating spermatogonial cells in vivo

(2008) Suveera Dhup et al.   NATURE METHODS