Article
Multidisciplinary Sciences
Lijuan Zhao, Haibo Xie, Yunsi Kang, Yiwen Lin, Gai Liu, Miho Sakato-Antoku, Ramila S. Patel-King, Bing Wang, Cuihong Wan, Stephen M. King, Chengtian Zhao, Kaiyao Huang
Summary: CYB5D1 protein plays a crucial role in the coordinated beating of multiple cilia by regulating the redox state of cilia.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Francesco Chiuso, Rossella delle Donne, Giuliana Giamundo, Laura Rinaldi, Domenica Borzacchiello, Federica Moraca, Daniela Intartaglia, Rosa Iannucci, Emanuela Senatore, Luca Lignitto, Corrado Garbi, Paolo Conflitti, Bruno Catalanotti, Ivan Conte, Antonio Feliciello
Summary: Bardet-Biedl syndrome (BBS) is a ciliopathy characterized by retinal degeneration, obesity, renal abnormalities, postaxial polydactyly, and developmental defects. The E3 ubiquitin ligase PJA2 has been identified as a regulator of the BBSome, an octameric complex that controls ciliary trafficking. Ubiquitylation of BBS1 by PJA2 stabilizes the BBSome and promotes its binding to BBS3, leading to proper ciliary membrane targeting. Disruption of PJA2 or expression of a ubiquitylation-defective BBS1 mutant affects GPCR trafficking and gene transcription, recapitulating the BBS phenotype in a medaka fish model.
Article
Multidisciplinary Sciences
Luke A. Parry, Rudy Lerosey-Aubril, James C. Weaver, Javier Ortega-Hernandez
Summary: Ctenophores, predatory macroinvertebrates, have a controversial phylogenetic position with competing theories on the evolution of animal organ systems. Recent discoveries of two ctenophore species in Cambrian Utah shed light on the early evolution of nervous and sensory features in the phylum, showing a substantial complexity in the nervous system of Cambrian ctenophores.
Article
Zoology
Guillermo San Martin, Patricia Alvarez-Campos, Yusuke Kondo, Jorge Nunez, Maria Ana Fernandez-Alamo, Fredrik Pleijel, Freya E. Goetz, Arne Nygren, Karen Osborn
Summary: A new genus of ectoparasitic marine annelids, Ctenophoricola gen. nov., living on ctenophores, has been described with unique body structures and eye features. Two new species have been identified, differing mainly in coloration, shape of parapodia, number of chaetae, and body ciliation.
ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY
(2021)
Article
Biology
Suzanne H. Hodge, Amy Watts, Richard Marley, Richard A. Baines, Ernst Hafen, Lindsay K. MacDougall
Summary: Primary cilia are compartmentalised from the rest of the cell by a ciliary gate, allowing selective import and export of molecules for assembly, function, and maintenance. The protein twitchy plays a crucial role in both compartmentalised sensory cilia and cytoplasmic sperm flagella.
Article
Biochemistry & Molecular Biology
Ewa Stypulkowski, Qiang Feng, Ivor Joseph, Victoria Farrell, Juan Flores, Shiyan Yu, Ryotaro Sakamori, Jiaxin Sun, Sheila Bandyopadhyay, Soumyashree Das, Radek Dobrowolski, Edward M. Bonder, Miao-Hsueh Chen, Nan Gao
Summary: The research reveals the crucial role of Rab8 in differentiation of mesenchymal stem cells into adipocytes by controlling signaling pathways and vesicular compartments to promote proper attenuation of Wnt signaling, thereby regulating adipogenesis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Medicine, Research & Experimental
Guang-Jie Zhu, Yuhang Huang, Linqing Zhang, Keji Yan, Cui Qiu, Yihan He, Qing Liu, Chengwen Zhu, Matias Morin, Miguel Angel Moreno-Pelayo, Min-Sheng Zhu, Xin Cao, Han Zhou, Xiaoyun Qian, Zhigang Xu, Jie Chen, Xia Gao, Guoqiang Wan
Summary: CGN is a cytoskeleton-associated protein that regulates RhoA activity and is found at the apical junctions of epithelial cells. A truncating variant of CGN has been identified as a pathogenic variant for autosomal dominant non-syndromic hearing loss, leading to reduced expression and abnormal localization of CGN, as well as impaired morphology of cochlear hair cell structures and auditory function.
EMBO MOLECULAR MEDICINE
(2023)
Article
Endocrinology & Metabolism
Christopher R. Paradise, M. Lizeth Galvan, Oksana Pichurin, Sofia Jerez, Eva Kubrova, S. Sharare Dehghani, Margarita E. Carrasco, Roman Thaler, A. Noelle Larson, Andre J. van Wijnen, Amel Dudakovic
Summary: The research demonstrates that Brd4 is a key epigenetic regulator required for normal chondrogenesis and endochondral ossification, with loss of Brd4 leading to abnormal cartilage development in mice and delayed long bone formation.
Article
Cell Biology
Karyn Jourdeuil, Karen M. Neilson, Helene Cousin, Andre L. P. Tavares, Himani D. Majumdar, Dominique Alfandari, Sally A. Moody
Summary: Zmym4 plays an important role in regulating embryonic cranial gene expression, and its loss results in altered early cranial gene expression as well as abnormal craniofacial cartilage morphology.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Plant Sciences
Deepika Sharma, Dhanraj Singh, Kirti Singh, Aditi Dwivedi, Aashish Ranjan, Alok Krishna Sinha
Summary: Light is a crucial environmental factor in plant growth and development. PIFs are negative regulators involved in light responses and are essential for hypocotyl elongation in Arabidopsis. This study found that MPK6 phosphorylates and interacts with PIF3, and the phosphorylation affects its binding to miR163 promoter. Overexpression of PIF3 influences the expression of RL-responsive miRNAs and genes involved in photomorphogenesis. These results suggest that the MPK6-PIF3 module plays a role in connecting RL-responsive miRNAs and PIF3-regulated genes.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Multidisciplinary Sciences
Linhui Yu, Jilian Fan, Chao Zhou, Changcheng Xu
Summary: Lipid droplet biogenesis occurs at specific sites in the endoplasmic reticulum and is regulated by a specific set of lipids and proteins. Sterols are shown to play a crucial role in coordinating oil and oleosin biosynthesis for the formation of lipid droplets in plants such as Arabidopsis.
NATURE COMMUNICATIONS
(2021)
Article
Environmental Sciences
Su Shiung Lam, Shin Ying Foong, Bernard H. K. Lee, Felicia Low, Aage K. O. Alstrup, Yong Sik Ok, Wanxi Peng, Christian Sonne
Summary: Global warming is causing a reduction in Arctic sea ice, leading to energetic stress on key predatory species such as polar bears and narwhals, which is already threatening the biodiversity and indigenous people of the vulnerable region. The increase in shipping activities in the Arctic gateway is adding further pressure to marine mammals, potentially destabilizing the global climate further. International governance through the establishment of Arctic International National Parks and Marine Protected Areas is necessary for sustainable use of the Arctic Ocean and adjacent waters.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Plant Sciences
Yasuyo Yamaoka, Seungjun Shin, Yuree Lee, Masaki Ito, Youngsook Lee, Ikuo Nishida
Summary: Phosphatidylserine (PS) plays a crucial role in root cytokinesis in Arabidopsis by mediating cargo vesicular trafficking required for cell plate formation, as the lack of PS leads to a delay in telophase progression during cell division.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Cell Biology
Miho Sakato-Antoku, Stephen M. King
Summary: In this study, a Chlamydomonas LC1 null mutant (dlu1-1) was characterized. The mutant showed reduced swimming velocity and beat frequency, loss of hydrodynamic coupling between the cilia, and disrupted kinetics of cytoplasmic preassembly of axonemal dyneins. Furthermore, loss of LC1 and I1/f resulted in the inability to build cilia under normal conditions and the absence of ciliary extension in response to lithium treatment. Overall, LC1 plays a crucial role in the maintenance of axonemal stability.
MOLECULAR BIOLOGY OF THE CELL
(2023)
Article
Cell Biology
Yukiko Imai, Kenji Saito, Kazumasa Takemoto, Fabien Velilla, Toshihiro Kawasaki, Kei-ichiro Ishiguro, Noriyoshi Sakai
Summary: The mutation in the zebrafish gene encoding the transverse filament protein Sycp1 leads to incomplete pairing of homologs in zebrafish meiosis, while not affecting double-strand break formation near telomeres.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Plant Sciences
Sakiko Ishida, Hidemasa Suzuki, Aya Iwaki, Shogo Kawamura, Shohei Yamaoka, Mikiko Kojima, Yumiko Takebayashi, Katsushi Yamaguchi, Shuji Shigenobu, Hitoshi Sakakibara, Takayuki Kohchi, Ryuichi Nishihama
Summary: In this study, it was demonstrated that in the liverwort Marchantia polymorpha, the decrease in endogenous auxin level at the cut surface of decapitated explants induced the expression of a key transcription factor gene, MpLAXR. The expression of MpLAXR was able to overcome the inhibition of regeneration by exogenously applied auxin, and ectopic expression of MpLAXR promoted cell proliferation in normally quiescent tissues. These findings provide insights into stem cell regulation and apical dominance establishment in land plants.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Shuji Shigenobu, Yoshinobu Hayashi, Dai Watanabe, Gaku Tokuda, Masaru Y. Hojo, Kouhei Toga, Ryota Saiki, Hajime Yaguchi, Yudai Masuoka, Ryutaro Suzuki, Shogo Suzuki, Moe Kimura, Masatoshi Matsunami, Yasuhiro Sugime, Kohei Oguchi, Teruyuki Niimi, Hiroki Gotoh, Masaru K. Hojo, Satoshi Miyazaki, Atsushi Toyoda, Toru Miura, Kiyoto Maekawa
Summary: Termites are social organisms with a polyphenic caste system, and gene duplication plays a crucial role in their social evolution. The duplicated genes in the termite genome are associated with caste-biased gene expression and diverse social functions, contributing to caste-specialized functions. Gene duplication facilitates social evolution through regulatory diversification, leading to caste-biased expression and subfunctionalization and/or neofunctionalization.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biology
Shuji Shigenobu, Shunta Yorimoto
Summary: This review summarizes recent progress in genomics of aphids and their symbionts, revealing pervasive gene duplication and chromosomal rearrangements, and identifying novel symbionts using modern deep-sequencing technologies.
CURRENT OPINION IN INSECT SCIENCE
(2022)
Article
Biology
Yui Uchida, Shuji Shigenobu, Hiroyuki Takeda, Chikara Furusawa, Naoki Irie
Summary: This study demonstrates that the phylotypic period, characterized by low potential for phenotypic variations, is developmentally stable and evolutionarily conserved. The results suggest that the intrinsic nature of organisms, specifically developmental stability, may limit the phenotypic variations and bias evolutionary outcomes.
Article
Developmental Biology
Takashi Takeuchi, Haruka Matsubara, Fumina Minamitani, Yukio Satoh, Sayo Tozawa, Tomoki Moriyama, Kohei Maruyama, Ken-ichi T. Suzuki, Shuji Shigenobu, Takeshi Inoue, Koji Tamura, Kiyokazu Agata, Toshinori Hayashi
Summary: This study generated mutants of Hox13 genes using the CRISPR/Cas9 system in newts and found that Hox13 genes are essential for digit formation in both development and regeneration. The predominant role of Hoxa13 in newt limbs, unlike in mouse limbs, suggests a potential contribution of Hoxa13 in the transition from fins to limbs. These findings demonstrate the conservation of Hox13 gene function in limb development and regeneration across taxa.
Article
Biochemistry & Molecular Biology
Chen-yo Chung, Shuji Shigenobu
Summary: Differentiation of the non-reproductive caste is a unique feature of eusocial insects, and apoptosis in oocytes plays a major role in constraining their reproductivity. This study investigated the soldier sterility in a hemipteran insect, the social aphid Ceratovacuna japonica, and found that soldiers possess ovaries and germaria but their ovarioles are small and lacking gastrulating embryos. The study also revealed apoptosis in the maternal nutritive cells of soldier ovaries and developmental failure of embryos.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Evolutionary Biology
Hajime Yaguchi, Shogo Suzuki, Naoto Kanasaki, Yudai Masuoka, Ryutaro Suzuki, Ryouhei H. Suzuki, Yoshinobu Hayashi, Shuji Shigenobu, Kiyoto Maekawa
Summary: In this study, the researchers investigated the role of vitellogenin (Vg) in nonreproductive castes of termites. They found that Vg4 plays an important role in the nonreproductive functions of termites. This suggests that Vg genes have undergone functionalization during the eusocial transition of termites.
JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION
(2023)
Article
Multidisciplinary Sciences
Satoshi Ishishita, Shumpei Kitahara, Mayuko Takahashi, Sakura Iwasaki, Shoji Tatsumoto, Izumi Hara, Yoshiki Kaneko, Keiji Kinoshita, Katsushi Yamaguchi, Akihito Harada, Yasushige Ohmori, Yasuyuki Ohkawa, Yasuhiro Go, Shuji Shigenobu, Yoichi Matsuda, Takayuki Suzuki
Summary: This study investigated the molecular basis of low protoporphyrin IX (PPIX) production in the uterus of a white eggshell phenotype in Japanese quails, using transcriptome analysis and genetic linkage analysis. The results showed that several genes involved in heme biosynthesis, including ALAS1, HEPHL1, and PITX3, were specifically upregulated in the wild-type uterus and downregulated in the mutant. Further biochemical analysis indicated that the restricted availability of 5'-aminolevulinic acid is the main cause of low PPIX production. These findings suggest that uterus-specific transcriptional regulation of heme-biosynthesis-related genes plays an important role in eggshell pigment production in Japanese quails.
Article
Multidisciplinary Sciences
Tomonari Nozaki, Shuji Shigenobu
Summary: It has been discovered that aphid bacteriocytes exhibit hyper polyploidy, with different ploidy levels observed in different developmental stages and sexes of aphids. This finding provides a foundation for understanding the functional roles and biological significance of insect bacteriocyte polyploidy.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Shunta Yorimoto, Mitsuru Hattori, Maki Kondo, Shuji Shigenobu
Summary: This study reports a co-obligate symbiosis in the eusocial aphid, Ceratovacuna japonica, involving a novel Arsenophonus sp. symbiont and the common obligate endosymbiont Buchnera aphidicola. Both symbionts share anatomical and genomic properties similar to independently evolved multi-partner symbiotic systems. Additionally, symbiont populations and bacteriome morphology differ between reproductive and soldier castes.
Article
Genetics & Heredity
Yuuki Kobayashi, Tomoko F. Shibata, Hideki Hirakawa, Tomoaki Nishiyama, Akiyoshi Yamada, Mitsuyasu Hasebe, Shuji Shigenobu, Masayoshi Kawaguchi
Summary: Mycorrhizae are an important symbiotic relationship between plants and fungi, with ectomycorrhizae being the most common in boreal forest ecosystems. The genomic changes associated with the transition to the ectomycorrhizal lifestyle are not well understood.
Article
Biochemistry & Molecular Biology
Ryohei H. Suzuki, Takumi Hanada, Yoshinobu Hayashi, Shuji Shigenobu, Kiyoto Maekawa, Masaru K. Hojo
Summary: Termites have an intricate social system with cooperation and division of labor. The perception of chemical signals in this social system is still not well understood. This study identified chemosensory genes involved in signal transduction in termites and found that the expression levels of certain non-receptor genes are affected by both individual and collective behaviors among termite colony members.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Plant Sciences
Chihiro Miura, Yuki Furui, Tatsuki Yamamoto, Yuri Kanno, Masaya Honjo, Katsushi Yamaguchi, Kenji Suetsugu, Takahiro Yagame, Mitsunori Seo, Shuji Shigenobu, Masahide Yamato, Hironori Kaminaka
Summary: Orchids autoactivate the mycorrhizal symbiosis pathway through gibberellin inactivation to accept the fungal partner during seed germination.
Article
Genetics & Heredity
Yuki Yoshida, Nurislam Shaikhutdinov, Olga Kozlova, Masayoshi Itoh, Michihira Tagami, Mitsuyoshi Murata, Hiromi Nishiyori-Sueki, Miki Kojima-Ishiyama, Shohei Noma, Alexander Cherkasov, Guzel Gazizova, Aigul Nasibullina, Ruslan Deviatiiarov, Elena Shagimardanova, Alina Ryabova, Katsushi Yamaguchi, Takahiro Bino, Shuji Shigenobu, Shoko Tokumoto, Yugo Miyata, Richard Cornette, Takahiro G. Yamada, Akira Funahashi, Masaru Tomita, Oleg Gusev, Takahiro Kikawada
Summary: The study reveals the distinct functional specialization of Chromosome 4 in the sleeping chironomid for desiccation tolerance genes, as well as the high genetic turnover in this chromosome, enabling midges to rapidly adapt to harsh environments.
NAR GENOMICS AND BIOINFORMATICS
(2022)