Article
Pharmacology & Pharmacy
Ziyao Kang, Chunlan Zeng, Long Tian, Taoran Wang, Sen Yang, Qin Cheng, Jing Zhang, Qingbin Meng, Changhao Zhang, Zhao Meng
Summary: “This study designed a series of peptide-based gene delivery vectors decorated with T7 segment to enhance gene delivery efficiency by binding to transferrin receptors highly expressed on brain tumor cells. PT-02/DNA complexes showed the highest transfection efficiency in glioma cells with low cytotoxicity in normal cell lines, and it could transport DNA across the BBB model in vitro. Moreover, in vivo experiments demonstrated that PT-02/DNA could successfully deliver pIRES2-EGFP into the brain site of zebrafish. The designed peptide vectors offer a promising approach for gene therapy in glioma.”
Article
Polymer Science
Basudeb Mondal, Namita S. Mahadik, Rajkumar Banerjee, Sayam Sen Gupta
Summary: The development of end-functionalized shikimoylated-polypeptides offers a novel approach for targeted therapy of cancer cells, with the potential to deliver various biological entities into the nucleus. These polypeptides can enter mammalian cells through mannose-receptors and exclusively traffic into the nucleus, exhibiting a dual function of specific receptor binding and nuclear transport.
Article
Chemistry, Physical
Xiaomin Xu, Yang Liu, Yujie Yang, Jingjing Wu, Meiwen Cao, Li Sun
Summary: A facile and mild method for constructing peptide-modified AuNPs for gene delivery has been developed. The synthesized AuNPs have a high density of surface positive charges and can form condensed complexes with DNA, showing low cytotoxicity. The study paves a new way for synthesizing peptide-functionalized AuNPs for efficient gene delivery.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Chemistry, Multidisciplinary
Jiaojiao Zhang, Zixuan Wang, Jiwei Min, Xuelin Zhang, Rongxin Su, Yuefei Wang, Wei Qi
Summary: A transfection formulation is developed using an auxiliary lipid (DOTAP) added to the peptide, resulting in a transfection efficiency of 72.6% for pDNA, which is comparable to Lipofectamine 2000. The designed KHL peptide-DOTAP complex exhibits good biocompatibility and significantly improves mRNA delivery compared to KHL or DOTAP alone. The complex also achieves efficient endolysosomal escape, providing a new platform for enhancing peptide vector transfection efficiency.
Review
Biochemistry & Molecular Biology
Lidia Gurba-Bryskiewicz, Wioleta Maruszak, Damian A. Smuga, Krzysztof Dubiel, Maciej Wieczorek
Summary: This review discusses the concepts and applications of quality by design (QbD) and design of experiments (DOE) for optimizing lipid nanoparticles (LNPs) for RNA delivery. It highlights the latest trends, regulatory requirements, and advancements in mathematical and statistical design methods. QbD is described in a limited number of papers, while DOE is widely used in optimization works. Traditional statistical tests and modeling are being replaced by artificial intelligence and machine learning methods.
Article
Pharmacology & Pharmacy
Yoko Endo-Takahashi, Ryo Kurokawa, Kanako Sato, Nao Takizawa, Fumihiko Katagiri, Nobuhito Hamano, Ryo Suzuki, Kazuo Maruyama, Motoyoshi Nomizu, Norio Takagi, Yoichi Negishi
Summary: This study successfully enhanced the efficiency of delivering pDNA to neuronal cells crossing the blood-brain barrier by utilizing special peptides and polymer complexes, along with ultrasound and microbubble technology.
Review
Pharmacology & Pharmacy
Manon Berger, Anna Lechanteur, Brigitte Evrard, Geraldine Piel
Summary: RNA interference is a promising mechanism for gene silencing and disease treatment, but the stability of siRNA in blood is a challenge. Lipoplexes and LNPs are commonly used to deliver and protect siRNA, yet clinical translation remains difficult with only few RNAi drugs approved by the FDA. Innovative formulations incorporating pH-sensitive lipids, peptides, and polymers aim to enhance siRNA efficacy for effective antitumor effects in complex tumor environments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Chemistry, Physical
Mehri Karimi Jabali, Ali Reza Allafchian, Seyed Amir Hossein Jalali, Hamideh Shakeripour, Rezvan Mohammadinezhad, Fahime Rahmani
Summary: The study introduces the use of ascorbic acid-modified chitosan as a pDNA nanocarrier, demonstrating promising pDNA release in intestinal fluids and potential biocompatibility with cell lines Vero and EPC. Chitosan effectively shields pDNA in gastric environments and allows for release in alkaline intestinal tissues, presenting a novel approach for gene delivery through oral administration.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Biochemistry & Molecular Biology
Huiting Jia, Xindong Wang, Zhaoming Chen, Shuai Liu, Tongtong Zhou, Hao Zhou, Tianying Guo
Summary: Zinc ion complexes of dipicolylamine analogs have been used as a third component in the PEI1.8k/DNA gene delivery system to improve in vitro transfection performance, and the effects of different structural characteristics on the ternary complex have been systematically investigated. This ternary hybrid system provides an effective strategy to enhance gene delivery of cationic polymers.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Pharmacology & Pharmacy
Alexandru Chivu, Kantaraja Chindera, Graca Mendes, Angela An, Brian Davidson, Liam Good, Wenhui Song
Summary: This study demonstrates the efficient intracellular delivery of pDNA and ODNs using PHMB as a nano-vector, with comparable or even higher gene expression levels than PEI analogues in various cell lines. The well-established safety profile of PHMB makes it a promising candidate for further research and potential clinical applications.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2021)
Article
Nanoscience & Nanotechnology
Rong Ni, Ruilu Feng, Ying Chau
Summary: Nonviral vectors are a safer alternative to viral vectors for gene therapy, and artificial viruses combining viral and nonviral vector strengths have emerged as safe and efficient gene delivery vectors. In this study, peptide-DNA coassembled nanoparticles were developed as a new efficient vector for gene delivery, achieving high transfection efficiency through the introduction of rationally designed functional short peptide modules. These nanoparticles achieved over 90% transfection efficiency in a serum-containing medium. This work demonstrates the effectiveness of the module-based design approach and lays the groundwork for potential biomedical applications of peptide-based biomaterials.
ACS APPLIED NANO MATERIALS
(2023)
Review
Engineering, Biomedical
Yi Wang, Kiara F. Bruggeman, Stephanie Franks, Vini Gautam, Stuart I. Hodgetts, Alan R. Harvey, Richard J. Williams, David R. Nisbet
Summary: Gene delivery is crucial for treating genetic or epigenetic disorders, with efforts focused on improving the delivery and efficacy of viral gene therapy. Biomaterials offer a promising option for controlled and targeted gene delivery, potentially overcoming major challenges in current gene therapy approaches.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Pharmacology & Pharmacy
Emma M. McErlean, Cian M. McCrudden, John W. McBride, Grace Cole, Vicky L. Kett, Tracy Robson, Nicholas J. Dunne, Helen O. McCarthy
Summary: RALA, a cationic amphipathic peptide, has shown promise as an efficient nucleic acid delivery system. Rational peptide design was used to create six amphipathic peptides with potential for nucleic acid delivery, but not all were successful in transfection, highlighting the importance of peptide design for cellular uptake and endosomal escape. Pep2 showed similar characteristics to RALA but had issues with toxicity and lack of pH-responsiveness, making RALA the superior choice for non-toxic gene delivery.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Chemistry, Multidisciplinary
Naoto Yoshinaga, Takaaki Miyamoto, Masaki Odahara, Noriko Takeda-Kamiya, Kiminori Toyooka, Seia Nara, Haruna Nishimura, Feng Ling, Masayuki Su'etsugu, Minoru Yoshida, Keiji Numata
Summary: This study proposes a simple and effective mitochondrial gene delivery system that uses artificial peptides to guide exogenous mtDNA into mitochondria, enabling simultaneous multigene expression and restoration of mitochondrial functions. This technology is of great importance for mitochondrial engineering and clinical applications for mitochondrial diseases.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Materials Science, Biomaterials
Yu-xian Lai, Jia-yin Fu, Shao-fei Wu, Ren-yun Li, Jia-qi Hu, You-xiang Wang, M. Cristina L. Martins, Ke-feng Ren, Jian Ji, Guo-sheng Fu
Summary: Drug-coated balloon (DCB) is a therapeutic method for delivering antiproliferative drugs without permanent implants. However, delayed reendothelialization leads to poor therapeutic effects. The proposed PrS/pDNA/RAPA coating effectively promotes endothelial repair and inhibits neointimal hyperplasia after balloon-induced vascular injuries.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Polymer Science
Tania Albuquerque, Ana R. Neves, Telma Quintela, Diana Costa
Summary: The combination of novel anticancer delivery systems and the circadian clock has been shown to optimize cancer therapy by improving drug/gene delivery efficiency. This study demonstrates the influence of circadian rhythm on the targeting ability and transfection performance of PEI/pDNA/MTX carriers, providing insights into the optimal time for drug/gene delivery.
Article
Pharmacology & Pharmacy
Ruben Faria, Milan Paul, Swati Biswas, Eric Vives, Prisca Boisguerin, Angela Sousa, Diana Costa
Summary: Mitochondria have their own genome and mutations in mitochondrial DNA can lead to various diseases. Developing a gene delivery system specifically targeting mitochondria is crucial for mitochondrial gene therapy. This study compared polymer- and peptide-based systems and found that peptides have a better capability to complex with DNA, forming smaller particles suitable for cellular transfection and mitochondrial gene therapy.
Article
Pharmacology & Pharmacy
Ana Raquel Neves, Tania Albuquerque, Ruben Faria, Ana M. Goncalves, Cecilia Santos, Eric Vives, Prisca Boisguerin, Luis A. Passarinha, Angela Sousa, Diana Costa
Summary: Despite progress in cancer diagnosis and treatment, glioblastoma remains the most lethal brain tumor. Gene therapy focused on non-viral vectors, such as transferrin (Tf), has been developed as a promising approach. In this study, a cell-penetrating peptide (WRAP5) targeting Tf was used to condense tumor suppressor p53-encoding plasmid DNA (pDNA), forming nanocomplexes. These complexes showed efficient cellular internalization, gene expression, and decreased viability of glioblastoma cells.
Article
Biochemistry & Molecular Biology
Joao Batista-Silva, Diana Gomes, Jorge Barroca-Ferreira, Eugenia Gallardo, Angela Sousa, Luis A. Passarinha
Summary: This work demonstrates the potential of calcium- and nickel-crosslinked Gellan Gum (GG) microspheres to capture STEAP1 directly from complex lysates in a batch method. The calcium-crosslinked microspheres were applied in an ionic exchange strategy, while the nickel-crosslinked microspheres were applied in an affinity strategy. Both formulations showed small diameters, but the calcium-crosslinked microspheres were smoother. The most promising results were obtained for the ionic strategy, where calcium-crosslinked GG microspheres were able to completely bind DM-solubilized STEAP1 in lysate samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Salome Ruiz-Demoulin, Eva Trenquier, Sanaa Dekkar, Sebastien Deshayes, Prisca Boisguerin, Cesar Serrano, Pascal de Santa Barbara, Sandrine Faure
Summary: Gastrointestinal stromal tumor (GIST) is mainly caused by a KIT receptor tyrosine kinase oncogenic mutation. Targeting KIT using tyrosine kinase inhibitors provides benefit, but resistance eventually develops. This study shows that LIX1 is upregulated in response to imatinib or sunitinib treatment in GIST cells and silencing LIX1 enhances the anti-tumor effect of imatinib by inhibiting MAPK signaling reactivation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Miguel Ferreira, Diana Gomes, Miguel Neto, Luis A. Passarinha, Diana Costa, Angela Sousa
Summary: In this study, chitosan/sulfonyl-ether-beta-cyclodextrin (SBE-beta-CD)-conjugated delivery systems were used to improve the solubility and bioavailability of quercetin in cervical cancer cells. The formulations using high molecular weight chitosan and SBE-beta-CD showed the best results, with nanoparticle sizes of 272.07 +/- 2.87 nm, a polydispersity index (PdI) of 0.287 +/- 0.011, a zeta potential of +38.0 +/- 1.34 mV, and an encapsulation efficiency of approximately 99.9%. The delivery systems exhibited increased cytotoxic effect on HeLa cells, indicating a significant improvement in quercetin bioavailability.
Review
Pharmacology & Pharmacy
Ruben Faria, Prisca Boisguerin, Angela Sousa, Diana Costa
Summary: Mitochondria are crucial cellular organelles responsible for energy production in cells, and mutations in their DNA (mtDNA) can lead to various diseases. Mitochondrial gene therapy is a promising strategy to address these disorders. Developing efficient mtDNA-based delivery systems to target and transfect mammalian mitochondria is an exciting area of research that can contribute to restoring normal mitochondrial function.
Review
Chemistry, Applied
D. Gomes, J. P. Batista-Silva, A. Sousa, L. A. Passarinha
Summary: Gellan gum, a biodegradable microbial exopolysaccharide, has potential applications in various fields such as food, pharmacy, biomedicine, and tissue engineering. Researchers have utilized the hydroxyl and carboxyl groups in each repeating unit to improve the properties of gellan gum, leading to significant advancements in the design of gellan-based materials.
CARBOHYDRATE POLYMERS
(2023)
Article
Engineering, Biomedical
Tania Albuquerque, Ana Raquel Neves, Milan Paul, Swati Biswas, Elena Vuelta, Ignacio Garcia-Tunon, Manuel Sanchez-Martin, Telma Quintela, Diana Costa
Summary: The circadian clock has a connection with cancer development/progression and considering circadian rhythm in anticancer drug therapy can enhance its effectiveness. Nanosized drug delivery systems have been proven to be suitable platforms for targeted drug release, and studying the relationship between chronobiology and nanotechnology can greatly improve cancer clinical outcomes.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Review
Biotechnology & Applied Microbiology
Miguel Ferreira, Diana Costa, Angela Sousa
Summary: This article introduces the encapsulation delivery systems of flavonoids, which have made progress in improving the stability and bioavailability of flavonoids. They have high therapeutic potential and are of great significance in cervical cancer therapy.
BIOENGINEERING-BASEL
(2022)
Article
Biophysics
Cristina Potrich, Anna Pedrotti, Cecilia Pederzolli, Lorenzo Lunelli
Summary: This study developed different functional surfaces for capturing exosomes and verified their effectiveness through experiments. Among them, the negatively-charged surface performed the best, capturing a large number of exosomes and successfully analyzing their biomarkers.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Brojendra Nath Saren, Srushti Mahajan, Mayur Aalhate, Rahul Kumar, Essha Chatterjee, Indrani Maji, Ujala Gupta, Santosh Kumar Guru, Pankaj Kumar Singh
Summary: This study developed P-selectin-targeted dasatinib nanoparticles coated with chitosan and fucoidan (DST-CH-FUC-NPs), which showed sustained release, reduced hemolytic potential, increased cytotoxicity and cellular uptake compared to free dasatinib. These nanoparticles also demonstrated enhanced ROS production, mitochondrial membrane potential damage, apoptosis induction, cell migration inhibition, and disruption of lysosomal membrane integrity.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Weiping Deng, Xiaohui Li, Ya Li, Zhongbing Huang, Yulin Wang, Ning Mu, Juan Wang, Tunan Chen, Ximing Pu, Guangfu Yin, Hua Feng
Summary: This study demonstrates the importance of chirality in nerve repair by constructing a GO-phenylalanine derivative hydrogel system. In vivo experiments show that the dextro group significantly improves functional recovery and histological restoration in rat sciatic nerve repair models. The doped GO promotes angiogenesis and myelination. These results suggest that chirality plays a crucial role in promoting nerve regeneration.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Xiaoyan Wang, Jin Gao, Yu Gao, Linlin Zhang, Congchao Xu, Qintong Li, Lin Li, Jianliang Xue
Summary: In this study, a highly effective surfactant producer strain, Bacillus Cereus GX7, was isolated from the oil tank bottom sludge of Shengli Oil Field in China. The biosurfactant produced by GX7 was identified as surfactin, a lipopeptide surfactant, through TLC, FT-IR, and LC-MS/MS analysis. The fermentation process of GX7 was optimized using single-factor experiments, focusing on the composition of fermentation medium and fermentation conditions. Glucose and peptone were found to be the best carbon and nitrogen sources, and the optimum temperature, inoculum amount, pH, rotation speed, and fermentation time for the strain were determined to be 30°C, 1%, 7.5, 150 rpm, and 48 h, respectively. After optimization, the surface tension and emulsification index of the fermentation broth were 26.84 mN/m and 57.84%, respectively. Furthermore, the biosurfactant produced by GX7 demonstrated good stability over a wide range of temperature, pH, and salt concentration.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Rodney Marcelo do Nascimento, Christine Grauby-Heywang, Houssem Kahli, Nesrine Debez, Laure Beven, Ivan Helmuth Bechtold, Touria Cohen Bouhacina
Summary: This article presents a theoretical model based on thermodynamic rules to assess the early stages of bacterial biofilm formation on different material surfaces. By utilizing morphological characteristics of bacteria and Atomic Force Microscopy images, the model generates a dataset of energetically minimized states, which can be correlated with bacterial adhesion states.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Filipa A. L. S. Silva, Licinia Timochenco, Raquel Costa-Almeida, Jose Ramiro Fernandes, Susana G. Santos, Fernao D. Magalhaes, Artur M. Pinto
Summary: The study demonstrates that by photoreducing nanosized graphene oxide using ultraviolet radiation, nanometric particles with high light-to-heat conversion efficiency and water stability can be obtained. These nanomaterials exhibit high absorption in the near-infrared region and show no cytotoxicity towards human cells, indicating their potential for safe therapy.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
D. Costa, V. De Matteis, F. Treso, G. Montani, M. Martino, R. Rinaldi, M. Corrado, M. Cascione
Summary: This review primarily discusses the relationship between contact lens discomfort (CLD) and the surface properties of contact lenses (CLs), specifically the coefficient of friction (CoF). The review emphasizes the importance of introducing a standardized protocol for measuring CoF and calls for a more precise evaluation of the relationship between surface properties and comfort in CLs users.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Debdip Brahma, Tamal Sarkar, Rupal Kaushik, Akshay Narayan Sarangi, Amar Nath Gupta
Summary: This in-vitro study evaluates the effect of reactive oxygen species (ROS) on the structural rearrangement of elastin. The results show that oxidative stress leads to a decrease in protein size and changes in secondary structure, potentially promoting protein aggregation. This study is important for therapeutics aiming to prevent elastin degradation and aging.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Xin Yang, Qiang Wang, Chaoxi Yan, Degang Huang, Yinchang Zhang, Huazheng He, Shouliang Xiong, Congming Li, Pingbo Chen, Tingjun Ye, Dan Hu, Lei Wang
Summary: This study presents a practical and effective strategy to improve osseointegration in patients with osteoporosis. By coating titanium implants with polydopamine followed by strontium modification, the bi-functional implants promote bone regeneration and immune regulation. The results show good biocompatibility, sustained release of strontium ions, and stable osseointegration between bone tissues and implants.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Sengwang Fu, Jianping Zhu, Zhijun Jiang, Yue Cao, Yufei Chen, Lihao Zhang, Sunlong Li, Weipeng Lu, Chengbin Miao, Qing He, Qi Li, Weixing Zhang, Lehao Ren, Yachun Li, Hongchao Shi, Cihui Liu
Summary: Effective management of biofluids is crucial for in vivo surgical interventions. Recent advances include self-sealing needles, drug-eluting stents, and shear-thinning hydrogels. However, complications associated with intestinal mucosal injury and secondary damage still persist. In this study, researchers developed an interpenetrating Janus wettability stent coating that enables unidirectional draining of excessive biofluid. They also demonstrated directional biofluid movement using a self-pumping dressing with potential applications in biofluid collection and disease diagnosis through metal ion detection. This integrated system presents an opportunity for designing wound dressings with effective biofluid management and metal ion detection capabilities.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Maryam Hassani, Mojtaba Kamankesh, Mazda Rad-Malekshahi, Kobra Rostamizadeh, Farhad Rezaee, Ismaeil Haririan, Seyed Mojtaba Daghighi
Summary: Bacterial adhesion and biofilm formation on the surface of biomaterial implants is a critical problem, and a polymer brush coating with antiadhesive and antimicrobial properties has proven to be highly effective in resolving this issue.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Huaihong Zhang, Na Liu, Yuting Zhang, Hui Cang, Zhaosheng Cai, Ziqun Huang, Jun Li
Summary: A functionalized cationic polymer, CR-PQAC, was designed and synthesized for photothermal enhanced antimicrobial therapy. The CR-PQAC nanoparticles exhibited significant antibacterial activity and low cytotoxicity against mammalian cells.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Ipsita Sahu, Priyadarshi Chakraborty
Summary: Peptide nanotechnology bridges the gap between materials and biological worlds by utilizing self-assembly of short-peptide building blocks. Hydrogels engineered from these short peptides show great potential in biomedical applications, but their weak mechanical properties and limited functional diversity need to be addressed. Nanoengineering the networks of these hydrogels by incorporating small molecules, polymers, and inorganic/carbon nanomaterials can enhance their mechanical properties and expand their functional diversity.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Hao Wei, Yajun Gu, Ao Li, Panpan Song, Dingding Liu, Feihu Sun, Xiaofeng Ma, Xiaoyun Qian
Summary: In this study, a stable three-dimensional conductive hydrogel was prepared by cross-linking MXenes to Matrigel hydrogel. The conductive hydrogel promotes the proliferation and differentiation of NSCs, providing new strategies for neural tissue engineering.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Yue Zhong, Xiaoli Ge, Juan Zhang, Qun Wei, Feng Wang, Yongke Zhong
Summary: The effect of oxygenic groups on the hemocompatibility of activated carbon was studied through liquid-phase oxidation and subsequent heat treatment. Results showed that the presence of oxygenic groups improved hemocompatibility, while their removal decreased it.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)