期刊
CHEMSUSCHEM
卷 12, 期 23, 页码 5142-5148出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cssc.201902413
关键词
biocatalysis; biotransformations; enzymes; gene expression; optogenetics
资金
- Ministry of Education (MOE) [MOE2017-T2-2-042]
- National Research Foundation and MOE Singapore under its Research Centre of Excellence Programme, Singapore Centre for Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore [M4330005.C70]
In green chemical synthesis, biofilms as biocatalysts have shown great promise. Efficient biofilm-mediated biocatalysis requires the modulation of biofilm formation. Optogenetic tools are ideal to control biofilms because light is noninvasive, easily controllable, and cost-efficient. In this study, a gene circuit responsive to near-infrared (NIR) light was used to modulate the cellular level of bis-(3 '-5 ') cyclic dimeric guanosine monophosphate (c-di-GMP), a central regulator of the prokaryote biofilm lifestyle, which allowed the regulation of biofilm formation by using NIR light. The engineered biofilm was applied to catalyze the biotransformation of indole into tryptophan in submerged biofilm reactors and NIR-light-enhanced biofilm formation resulted in an approximately 30 % increase in tryptophan yield, which demonstrates the feasibility of the application of light to modulate the formation and performance of catalytic biofilms for chemical production. The c-di-GMP-targeted optogenetic approach to modulate catalytic biofilms showcases applications for biofilm-mediated biocatalysis.
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