期刊
CELL PROLIFERATION
卷 52, 期 6, 页码 -出版社
WILEY
DOI: 10.1111/cpr.12703
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资金
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Jiangsu Planned Projects for Postdoctoral Research Funds [1601190C]
- China Postdoctoral Science Foundation [2016M591925]
- Natural Science Foundation of Jiangsu Province [BK20170263]
Objectives Interleukin-34 (IL-34) is associated with hepatitis B virus (HBV) infection and hepatocellular carcinoma (HCC). However, the role and associated mechanisms of IL-34 in HBV-related HCC remain unclear. In this study, the expression, biological function and associated mechanisms of IL-34 in HBV-related HCC cells were investigated. Methods IL-34 expression induced by HBV and HBV X (HBX) gene was measured in hepatoma cells. The role of CCAAT/enhancer-binding protein alpha (CEBP/alpha) in HBX-induced IL-34 expression was examined. The signal pathways involved in the expression of CEBP/alpha and IL-34 induced by HBX were assessed. The role of IL-34 in the proliferation and migration of HCC cells, and related mechanisms were explored. Results Dependent on HBX, HBV increased IL-34 expression in hepatoma cells, and HBX upregulated and interacted with CEBP/alpha to enhance the activity of IL-34 promoters. CEBP/alpha mediated by HBX was associated with the activation of PI3-K and NF-kappa B pathways to promote IL-34 expression. Via CSF1-R and CD138, IL-34 promoted the proliferation and migration of hepatoma cells, and contributed to the activation of ERK and STAT3 pathways and the upregulation of Bcl-xl and c-Myc mediated by HBX. Conclusion We demonstrate that IL-34 contributes to HBX-mediated functional abnormality of HCC cells and provides a novel insight into the molecular mechanism of carcinogenesis mediated by HBX.
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