期刊
CARBOHYDRATE POLYMERS
卷 222, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2019.115031
关键词
Chondroitin sulfate; Inflammation; Shrimp; Thrombin
资金
- CNPq (Conselho Nacional de Desenvolvimento e Tecnologico) [4561602014-0]
- PPG.BIOQ (Programa de Pos-graduacao em Bioquimica/UFRN)
- CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
- FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo) [2017/14179-3, 2015/03964-6]
- FINEP (Financiadora de Estudos e Projetos) [01/2013 -736/13]
- BBSRC (Biotechonology and Biological Sciences Research Council)
- CAPES
- PDSE (Programa de Doutorado Sanduiche no Exterior) [88881.187624/2018]
- BBSRC [1945155] Funding Source: UKRI
The detailed structure of a further Chondroitin Sulfate from Litopenaeus vannamei shrimp (sCS) is described. The backbone structure was established by H-1/C-13 NMR, which identified 3-O-sulfated GlcA, 4-O-sulfated GalNAc, 6-O-sulfated GalNAc, and 4,6-di-O-sulfated GalNAc residues. GlcA is linked to GalNAc 4,6 di S and GlcA 3S is linked to GalNAc 4S, GalNAc 4,6 di-S and GalNAc6S residues. The anticoagulant properties of this sCS were evaluated by activated partial thromboplastin time, anti-IIa, anti-Xa and anti-heparin cofactor II-mediated activities, and sCS failed to stabilise antithrombin in a fluoresence shift assay. The anti-inflammatory effect of sCS was explored using a model of acute peritonitis, followed by leukocyte count and measurement of the cytokines, IL-1 beta, IL-6 and TNF-alpha. The compound showed low clotting effects, but high anti-IIa activity and HCII-mediated thrombin inhibition. Its anti-inflammatory effect was shown by leukocyte recruitment inhibition and a decrease in pro-inflammatory cytokine levels. Although the biological role of sCS remains unknown, its properties indicate that it is suitable for studies of multi-potent molecules obtained from natural sources.
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