Article
Plant Sciences
Rocio Reyero-Saavedra, Sara Isabel Fuentes, Alfonso Leija, Gladys Jimenez-Nopala, Pablo Pelaez, Mario Ramirez, Lourdes Girard, Timothy G. Porch, Georgina Hernandez
Summary: This study aims to isolate and characterize symbiotic mutants in common bean (Phaseolus vulgaris) in order to understand the symbiotic nitrogen fixation (SNF) process in this important food crop. Three non-nodulating mutants were identified and their impairments in different symbiotic steps were characterized. The responsible mutated gene is being mapped for further understanding of SNF in common bean.
Article
Plant Sciences
Arantza del-Canto, Alvaro Sanz-Saez, Anna Sillero-Martinez, Eider Mintegi, Maite Lacuesta
Summary: Drought is a major challenge in agriculture due to its negative impact on crop growth and yield, but inoculating pulses with rhizobium strains can improve drought tolerance. This study evaluated the ability of indigenous rhizobia strains from nearby crop fields to enhance drought tolerance in common bean plants. Three strains were found to be highly efficient under drought conditions, showing better infectiveness and effectiveness compared to reference strains and chemically fertilized plants. These results provide a basis for developing efficient common bean inoculants to increase crop yield under drought conditions in Northern Spain.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Fernando de Souza Buzo, Lucas Martins Gare, Nayara Fernanda Siviero Garcia, Maura Santos Reis de Andrade da Silva, Pedro Henrique Giova da Silva, Pamela Roberta de Souza Morita, Juliana Barboza Correa, Juliana Trindade Martins, Everlon Cid Rigobelo, Amaia Nogales, Orivaldo Arf
Summary: Chemical seed treatment and mycorrhizal inoculation benefit bean plants and their nutritional status. The best combinations for the bean crop were metalaxyl + fludioxonil + tiabendazole with 41.4 mg of the inoculant per 100 seeds and pyraclostrobin + thiophanate methyl + fipronil with 62.1 mg of the inoculant per 100 seeds.
PEST MANAGEMENT SCIENCE
(2022)
Article
Microbiology
Jesus Lirio-Paredes, Katty Ogata-Gutierrez, Doris Zuniga-Davila
Summary: The study isolated Rhizobium strains from soil samples collected from coffee fields, and inoculated them on red kidney beans. The results showed that the strain RZC12 significantly improved plant growth, development, and yield of bean plants.
Article
Plant Sciences
Ioannis Karavidas, Georgia Ntatsi, Theodora Ntanasi, Anastasia Tampakaki, Ariadni Giannopoulou, Dimitra Pantazopoulou, Leo Sabatino, Pietro P. M. Iannetta, Dimitrios Savvas
Summary: This study aims to reduce the use of inorganic nitrogen fertilizer in hydroponic common bean cultivation without compromising plant performance. The 50-25% mineral-N application regime showed significant increases in nodulation, biological nitrogen fixation (BNF), and yield, while the 75-25% regime during the vegetative stage resulted in yield losses due to restricted nodulation and BNF. Both rhizobia strains stimulated nodulation and BNF, but their capacity was suppressed as the inorganic-N input increased.
Article
Plant Sciences
Angeliki Galeou, Chrysanthi Stefanatou, Anastasia Prombona
Summary: As sessile organisms, plants need to adjust their immune responses to external attacks. This study investigated the effect of water, flagellin 22 (flg22), and a pathogenic bacterial strain on the transcriptional changes and oxidative burst generation in bean plants. Results showed that microbe-triggered response had a greater and longer-lasting impact compared to elicitor-triggered response. Infection also affected the expression of clock genes and specific oxidases, indicating a circadian rhythm in plant defense.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Chang Liu, Xiaoxu Yang, Zhishan Yan, Dajun Liu, Guojun Feng
Summary: This study reveals the genetic factors and pathways responsible for the purple appearance in snap bean through transcriptome and metabolome analyses. The increased expression of a key enzyme was found to be responsible for the purple color. These findings lay a foundation for further studies on the molecular mechanism of anthocyanin synthesis in snap bean and provide a framework for breeding different colors of snap bean.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Raul Davila-Delgado, Karen Flores-Canul, Marco Adan Juarez-Verdayes, Rosana Sanchez-Lopez
Summary: PvSYMRK-EGFP undergoes constitutive and rhizobia-induced endocytosis, which is regulated by the phosphorylation status, the endocytic motif YXXo, and the kinase activity of the receptor. These findings suggest that rhizobia-induced endocytosis modulates the duration and amplitude of the SYMRK signaling pathway.
Article
Multidisciplinary Sciences
Jinge Liu, Ting Wang, Qiulin Qin, Xiaocheng Yu, Shengming Yang, Randy D. Dinkins, Anett Kuczmog, Peter Putnoky, Artur Muszynski, Joel S. Griffitts, Attila Kereszt, Hongyan Zhu
Summary: Plants have evolved the ability to distinguish between symbiotic and pathogenic microbial signals. The NS1 locus in Medicago truncatula can block the invasion and nodule induction by Sinorhizobium meliloti. The NS1 locus is controlled by two genes, NS1a and NS1b, which can regulate the nodulation blockade of specific bacterial strains.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Elizabeth Monroy-Morales, Raul Davila-Delgado, Emmanuel Ayala-Guzman, Alicia Gamboa-deBuen, Rosana Sanchez-Lopez
Summary: The development of symbiotic nitrogen-fixing nodules in legumes involves infection and organogenesis, which are well-coordinated yet their dynamics remain unclear. In this study, the connection between infection thread (IT) and cortical cell division (CCD) was visualized in Phaseolus vulgaris nodulation. It was found that the IT exits the root hair to penetrate a subepidermal cortical cell when cytokinesis is concluding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Minglong Li, Yuye Wang, Pengmin Zhang, Chunxu Bai, Lei Cao, Ludan Li, Jihong Jiang, Xiaodong Ding, Jialei Xiao
Summary: In this study, the authors identified GmNodH as an interacting protein of GmSnRK1 in soybean. They found that GmNodH is highly expressed in soybean roots and can be induced by rhizobial infection and nitrate stress. They also demonstrated that GmSnRK1 can phosphorylate GmNodH and that co-expression of GmSnRK1/GmNodH genes promotes soybean nodulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Paula Rodrigues Oblessuc, David F. Bridges, Maeli Melotto
Summary: In this study, an RNA sequencing analysis was conducted to investigate the response of common bean to halo blight caused by Pseudomonas syringae pv. phaseolicola (Pph). The results revealed differentially expressed genes that are involved in plant immune responses and mapped to known Pph resistance loci or quantitative trait loci. These findings suggest that the identified genes may play a role in conferring resistance to halo blight in common bean.
Article
Plant Sciences
Citlali Fonseca-Garcia, Noreide Nava, Miguel Lara, Carmen Quinto
Summary: The study reveals that PvRbohB plays a central role in root nodule symbiosis by regulating infection thread formation and nodule organogenesis through the production of ROS, which interact with flavonoids, carbon metabolism, cell cycle regulation, and plant hormones in the early stages.
Article
Microbiology
Judith Van Dingenen, Sonia Garcia Mendez, Stien Beirinckx, Lena Vlaminck, Annick De Keyser, Naomi Stuer, Severine Verschaete, Alexander Clarysse, Joke Pannecoucque, Stephane Rombauts, Isabel Roldan-Ruiz, Anne Willems, Sofie Goormachtig
Summary: Soybeans used for food and feed in Europe have negative impacts due to imports, leading to the need for increased local production and the search for indigenous nodulating strains in Belgium. The study highlights the diverse bacterial richness and the necessity of combining different identification techniques to capture the true nodule rhizobial community. Genomic analysis revealed multiple genetically distinct species capable of nodulating soybeans in laboratory conditions.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Plant Sciences
Anna B. Kitaeva, Artemii P. Gorshkov, Pyotr G. Kusakin, Alexandra R. Sadovskaya, Anna V. Tsyganova, Viktor E. Tsyganov
Summary: Plant cell differentiation is based on rearrangements of the tubulin cytoskeleton. This study investigated the bacteroid morphology and dynamics of the tubulin cytoskeleton in determinate nodules of four legume species. The results showed common patterns of tubulin cytoskeleton in the determinate nodules of the studied species, with species-specific differences associated with the organization of cortical microtubules in uninfected cells.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Blanca Jazmin Reyes-Hernandez, Svetlana Shishkova, Rachel Amir, Aranza Xhaly Quintana-Armas, Selene Napsucialy-Mendivil, Rocio Guadalupe Cervantes-Gamez, Hector Hugo Torres-Martinez, Jesus Montiel, Christopher D. Wood, Joseph G. Dubrovsky
JOURNAL OF EXPERIMENTAL BOTANY
(2019)
Article
Microbiology
Victor M. Flores-Nunez, Citlali Fonseca-Garcia, Damaris Desgarennes, Emiley Eloe-Fadrosh, Tanja Woyke, Laila P. Partida-Martinez
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Yolanda Ortega-Ortega, Janet Carrasco-Castilla, Marco A. Juarez-Verdayes, Roberto Toscano-Morales, Citlali Fonseca-Garcia, Noreide Nava, Luis Cardenas, Carmen Quinto
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Genetics & Heredity
Jorge Solis-Miranda, Citlali Fonseca-Garcia, Noreide Nava, Ronal Pacheco, Carmen Quinto
Article
Plant Sciences
Citlali Fonseca-Garcia, Noreide Nava, Miguel Lara, Carmen Quinto
Summary: The study reveals that PvRbohB plays a central role in root nodule symbiosis by regulating infection thread formation and nodule organogenesis through the production of ROS, which interact with flavonoids, carbon metabolism, cell cycle regulation, and plant hormones in the early stages.
Article
Biochemistry & Molecular Biology
Citlali Fonseca-Garcia, Claudia Marina Lopez-Garcia, Ronal Pacheco, Elisabeth Armada, Noreide Nava, Rocio Perez-Aguilar, Jorge Solis-Miranda, Carmen Quinto
Summary: This study analyzed the role of the PvMT1A gene in the Phaseolus vulgaris-Rhizobium tropici symbiosis. The results showed that PvMT1A plays an important role in the infection process and nodule development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mohammad Zarrabian, Jesus Montiel, Niels Sandal, Shaun Ferguson, Haojie Jin, Yen-Yu Lin, Verena Klingl, Macarena Marin, Euan K. James, Martin Parniske, Jens Stougaard, Stig U. Andersen
Summary: The research reveals that Lotus burttii has a higher promiscuity in interactions with rhizobia compared to Lotus japonicus, allowing it to form nodules with rhizobia from five different genera. The mapping of the Gifu/burttii promiscuity quantitative trait locus (QTL) to the same genetic locus regardless of the rhizobial genus suggests a common genetic mechanism for symbiont-range expansion.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2022)
Review
Plant Sciences
Johan Quilbe, Jesus Montiel, Jean-Francois Arrighi, Jens Stougaard
Summary: The establishment of root-nodule symbiosis in legumes can occur through either root hair infection threads or intercellular passage of bacteria. Research has shown that the intercellular infection mechanism is still poorly understood, but recent discoveries have shed some light on this process.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Jesus Montiel, Ivette Garcia-Soto, Euan K. James, Dugald Reid, Luis Cardenas, Selene Napsucialy-Mendivil, Shaun Ferguson, Joseph G. Dubrovsky, Jens Stougaard
Summary: This study demonstrates the crucial role of the regulation of aromatic amino acid biosynthesis in sustaining root hair growth and establishing symbiotic relationships in the legume Lotus japonicus. The enzyme DAHPS1 is found to be critical for root hair development and for the symbioses with both rhizobial and mycorrhizal microbes. Mutants lacking DAHPS1 show altered root hair morphology, reduced symbiotic interactions, and downregulation of cell wall-related genes.
Article
Plant Sciences
Jesus Montiel, Dugald Reid, Thomas H. Gronbaek, Caroline M. Benfeldt, Euan K. James, Thomas Ott, Franck A. Ditengou, Marcin Nadzieja, Simon Kelly, Jens Stougaard
Summary: This study reports intercellular infection of the model legume Lotus japonicus by the IRBG74 strain and reveals the genetic requirements and differences in the pathways governing this process compared to intracellular infection. The results indicate a differential requirement for cytokinin signaling during intercellular rhizobial entry, highlighting distinct modalities of inter- and intracellular infection mechanisms in L. japonicus.
Article
Agronomy
Citlali Fonseca-Garcia, Jorge Solis-Miranda, Ronal Pacheco, Carmen Quinto
Summary: LTPs are a large protein family in plants with key functions in both biotic and abiotic stress. They act as regulators of defense responses in plants-pathogen interactions. However, their roles in symbiotic interactions, especially in root nodule symbiosis, are still poorly understood.
FRONTIERS IN AGRONOMY
(2021)
Proceedings Paper
Agronomy
Jesus Montiel, Carmen Quinto
MICROBIAL PROBIOTICS FOR AGRICULTURAL SYSTEMS: ADVANCES IN AGRONOMIC USE
(2019)
Review
Agronomy
Jesus Montiel, Citlali Fonseca-Garcia, Carmen Quinto