Article
Virology
Guanping Chen, Yuedong Li, Xiangshuo Kong, Shudi Zhao, Jiale Li, Xiaofeng Wu
Summary: This study found that the expression of Bombyx mori HEXIM1 (BmHEXIM1) was significantly upregulated after BmNPV infection. By suppressing BmRelish-driven immune responses, BmHEXIM1 assisted in the immune escape of BmNPV. This research provides new insights into the mechanisms of immune escape of BmNPV and theoretical guidance for the breeding of BmNPV-resistant silkworm varieties.
Article
Entomology
Zhihao Su, Chunxiao Zhao, Xinming Huang, Junli Lv, Ziqin Zhao, Kaiyi Zheng, Xia Sun, Sheng Qin, Xueyang Wang, Byung-Rae Jin, Yangchun Wu
Summary: This study found that the hormone receptor BmEcR-B1 in silkworms is more critical than BmEcR-A in responding to BmNPV infection. Further investigation confirmed that BmEcR-B1 plays an anti-BmNPV role by participating in 20E-induced apoptosis. This study provides theoretical support for understanding the resistant mechanism of silkworms to BmNPV and controlling BmNPV disease.
Review
Physiology
Mian Muhammad Awais, Muhammad Shakeel, Jingchen Sun
Summary: miRNAs are small non-coding RNAs of about 22 nucleotides that have been reported to regulate gene expression at the posttranscriptional level, involved in various biological processes such as immunity, development, metabolism, and host-pathogen interactions. Both cellular and viral miRNAs may play vital roles in host-pathogen interactions, with viral infections altering host miRNA expression profiles.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Jing Xu, Xiaole Xie, Qiuqin Ma, Lu Zhang, Yu Li, Yin Chen, Kang Li, Yang Xiao, Gianluca Tettamanti, Hanhong Xu, Ling Tian
Summary: This study characterized the molecules involved in Bombyx mori nucleopolyhedrovirus (BmNPV)-silkworm interaction by RNA sequencing. The upregulated genes were mainly involved in translation, signal transduction, folding, sorting, and degradation, while the downregulated genes were predominantly related to metabolism. Knockout of specific genes inhibited or promoted BmNPV proliferation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Weiwei Zhou, Jindie Hong, Jinying Han, Fuchuan Cai, Qi Tang, Qian Yu, Guohui Li, Shangshang Ma, Xiaoyong Liu, Shuhao Huo, Keping Chen, Feifei Zhu
Summary: In this study, the role of glycosaminoglycan modifications, including heparan sulfate (HS) and chondroitin sulfate (CS), in Bombyx mori nuclear polyhedrosis virus (BmNPV) infection was investigated. The results showed that HS and CS are important attachment factors for BmNPV and targeting them can effectively inhibit viral infection.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Qian Zhang, Yun-Fei Wu, Peng Chen, Tai-Hang Liu, Zhan-Qi Dong, Cheng Lu, Min-Hui Pan
Summary: Cdc37 is a molecular chaperone that plays an active role in the proliferation of BmNPV in silkworm cells. Studies have shown that Cdc37 interacts with Hsp90 to promote virus replication. Co-expression of Cdc37 and Hsp90 is more effective in enhancing virus proliferation than overexpression alone.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Entomology
Xiong Wang, Guangyu Ma, Feifei Ren, Mian Muhammad Awais, Jingchen Sun
Summary: Research has shown that Bombyx mori fatty acid binding protein 1 (BmFABP1) is expressed in silkmoth cells and in the tissues of silkmoth larvae, where it inhibits the replication of Bombyx mori nucleopolyhedrovirus (BmNPV). This study provides new insights into the potential antiviral role of BmFABP1 and its importance in breeding BmNPV-resistant transgenic silkmoths.
Article
Entomology
Qin Xiao, Zhan-Qi Dong, Yan Zhu, Qian Zhang, Xiu Yang, Miao Xiao, Peng Chen, Cheng Lu, Min-Hui Pan
Summary: Our study demonstrated that Bombyx mori nucleopolyhedrovirus (BmNPV) induces G2/M phase arrest in insect cells by downregulating BmCDK1 and BmCyclin B, essential for its replication. The interaction of BmNPV IAP1 with BmCDK1 was confirmed, suggesting a mechanism through which BmNPV impacts cell cycle progression. These findings enhance our understanding of virus-host interaction and provide a potential target gene linking apoptosis and the cell cycle.
Article
Biochemistry & Molecular Biology
Peng Chen, Min Cai, Yu-Jie Feng, Cong Li, Zhan-Qi Dong, Wen-Fu Xiao, Liang Tang, Yan Zhu, Ting Tian, Bo-Yuan Deng, Min-Hui Pan, Cheng Lu
Summary: Transcriptome sequencing revealed 23 differentially expressed lncRNAs associated with the mitochondrial apoptosis pathway in silkworm cells infected with BmNPV. It was demonstrated that LINC5438 inhibits apoptosis and promotes BmNPV proliferation, potentially through the regulation of key genes in the mitochondrial pathway.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Agronomy
Xin Wang, Jun-Li Lv, Shuang Cheng, Zhi-Hao Su, Sheng Qin, Xia Sun, Xu-Dong Tang, Qiu-Ning Liu, Mu-Wang Li, Xue-Yang Wang
Summary: This study elucidates the important role of BmE74A in promoting the proliferation of BmNPV by directly interacting with the virus. The high expression of BmE74A in eggs, hemolymph, and midgut, as well as the differential expression in different resistant strains, confirms its significance in viral infection.
PEST MANAGEMENT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Guobao Wang, Dandan Xu, Dingge Guo, Yuzhuo Zhang, Xiaoxi Mai, Baoren Zhang, Hui Cao, Shengxiang Zhang
Summary: In this study, the metabolomic changes in Bombyx mori following BmNPV infection were investigated, revealing alterations in carboxylic acids, fatty acyls, and glycerophospholipids in the hemolymph, fat body, and midgut. BmNPV infection also affected immune-related pathways, such as amino acid metabolism, phospholipase D signaling pathway, and lipid metabolism. This research provides insights into the antiviral mechanism of Bombyx mori during BmNPV infection.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lin-Bao Zhu, Zhi-Hao Huang, Sadaf Ayaz, Zhe-Xiao Guo, Bing Ling, Xi-Ya Chen, Shi-Huo Liu, Jia-Ping Xu
Summary: This study shows that BmNPV infection leads to time-dependent ROS accumulation, which promotes the expression of BmFerHCH. BmFerHCH inhibits BmNPV-induced ROS accumulation by chelating Fe2+, thereby regulating ROS-mediated autophagy and apoptosis and affecting BmNPV replication.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Yajie Zhu, Miao Hu, Jonas Ngowo, Xu Gao, Xi Chen, Huihui Yan, Wei Yu
Summary: Silkworm is an important insect model with biological significance and is crucial in sericulture and bioreactors. The Bombyx mori nucleopolyhedrovirus (BmNPV) is a principal pathogen of silkworm. Through studying the acetylation of BmAda3 after BmNPV infection, it was found that acetylation modification affected the stability of P53, promoting cellular apoptosis. The research revealed the regulatory role of BmAda3 in the process of BmNPV infection.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Fisheries
Xiu Shi, Yaxin Zhang, Tianchen Zhu, Nan Li, Sufei Sun, Min Zhu, Jun Pan, Zeen Shen, Xiaolong Hu, Xing Zhang, Chengliang Gong
Summary: The study utilized 16S rDNA sequencing and metabolomics to show significant alterations in gut microbiota and metabolites of silkworm midgut after BmNPV infection. Analysis revealed enrichment of specific pathways related to viral infection and metabolism, highlighting the relationship between digestive tract gut microbiota, metabolites, and host-virus interaction. These findings enhance understanding of viral infection links to midgut microbiota and metabolic activity in the silkworm model.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Immunology
Mengmeng Zhang, Shigang Fei, Junming Xia, Yeyuan Wang, Hongyun Wu, Xian Li, Yiyao Guo, Luc Swevers, Jingchen Sun, Min Feng
Summary: This study is the first to investigate the role of Sirtuins in insect-virus interactions. It found that silkworm Sirt2 and Sirt5 have antiviral functions against BmNPV, and silkworm Sirt5 enhances host antiviral response by activating the immune pathway mediated by Relish.
FRONTIERS IN IMMUNOLOGY
(2022)