期刊
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
卷 19, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12906-019-2735-x
关键词
Anti-inflammation; Heracleum moellendorffii; Inflammatory diseases; Inflammatory response
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2016R1D1A3B03931713, NRF-2018R1A6A1A03024862]
Background Heracleum moellendorffii roots (HM-R) have been long treated for inflammatory diseases such as arthritis, backache and fever. However, an anti-inflammatory effect and the specific mechanism of HM-R were not yet clear. In this study, we for the first time explored the anti-inflammatory of HM-R. Methods The cytotoxicity of HM-R against RAW264.7 cells was evaluated using MTT assay. The inhibition of NO and PGE(2) production by HM-R was evaluated using Griess reagent and Prostaglandin E-2 ELISA Kit, respectively. The changes in mRNA or protein level following HM-R treatment were assessed by RT-PCR and Western blot analysis, respectively. Results HM-R dose-dependently blocked LPS-induced NO and PGE(2) production. In addition, HM-R inhibited LPS-induced overexpression of iNOS, COX-2, IL-1 beta and IL-6 in RAW264.7 cells. HM-R inhibited LPS-induced NF-kappa B signaling activation through blocking I kappa B-alpha degradation and p65 nuclear accumulation. Furthermore, HM-R inhibited MAPK signaling activation by attenuating the phosphorylation of ERK1/2, p38 and JNK. HM-R increased nuclear accumulation of Nrf2 and HO-1 expression. However, NAC reduced the increased nuclear accumulation of Nrf2 and HO-1 expression by HM-R. In HPLC analysis, falcarinol was detected from HM-R as an anti-inflammatory compound. Conclusions These results indicate that HM-R may exert anti-inflammatory activity by inhibiting NF-kappa B and MAPK signaling, and activating ROS/Nrf2/HO-1 signaling. These findings suggest that HM-R has a potential as a natural material for the development of anti-inflammatory drugs.
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