期刊
BIOINFORMATICS
卷 33, 期 3, 页码 425-427出版社
OXFORD UNIV PRESS
DOI: 10.1093/bioinformatics/btw631
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类别
资金
- National Institute of Allergy and Infectious Diseases
- National Institutes of Health [U19AI109946, U19AI057266, U19AI082724, T32AI007090]
Motivation: The B-cell receptor enables individual B cells to identify diverse antigens, including bacterial and viral proteins. While advances in RNA-sequencing (RNA-seq) have enabled high throughput profiling of transcript expression in single cells, the unique task of assembling the fulllength heavy and light chain sequences from single cell RNA-seq (scRNA-seq) in B cells has been largely unstudied. Results: We developed a new software tool, BASIC, which allows investigators to use scRNA-seq for assembling BCR sequences at single-cell resolution. To demonstrate the utility of our software, we subjected nearly 200 single human B cells to scRNA-seq, assembled the full-length heavy and the light chains, and experimentally confirmed these results by using single-cell primer-based nested PCRs and Sanger sequencing.
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