Article
Chemistry, Analytical
Ruo Yuan, Ya-Qin Chai, Jia-Hang Li, Jia-Li Liu, Xiao-Long Zhang, Xiao-Chun Zhu
Summary: A novel ultrasensitive electrochemiluminescence (ECL) biosensor was developed using 2D Co3O4 nanosheets as a coreaction accelerator for the detection of miRNA-21. The Co3O4 nanosheets promoted the decomposition of H2O2 to generate more O2.-, which enhanced the ECL signals by reacting with luminol. Additionally, the biosensor achieved ultrasensitive detection of miRNA-21 through the self-assembly of highly ordered rolling DNA nanomachines.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Emily Kerr, Ryan Farr, Egan H. Doeven, Yi Heng Nai, Richard Alexander, Rosanne M. Guijt, Beatriz Prieto-Simon, Paul S. Francis, Megan Dearnley, David J. Hayne, Luke C. Henderson, Nicolas H. Voelcker
Summary: The sensor demonstrated in this study combines an ECL molecular beacon with magnetic bead based extraction of the miRNA target sequence to provide a sensitive and portable solution for miRNA-21 detection. The sensor does not require intercalating labels, reporter probes, added enzymes or hairpin primers for target amplification, offering a simple and robust strategy for miRNA detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Yanjie Zheng, Li Chen, Xinyang Yin, Fei Lin, Yichun Xu, Xinhua Lin, Shaohuang Weng
Summary: A dual-mode electrochemical biosensor has been developed for the sensitive detection of femtomolar miRNA-155 using electrochemiluminescence (ECL) and adsorptive stripping voltammetry (AdsSV). The sensor demonstrates high selectivity, reproducibility and long-term stability, providing a practical platform for rapid, simple, selective and reliable detection of miRNAs.
MICROCHEMICAL JOURNAL
(2021)
Article
Engineering, Environmental
Mengqi Pan, Weiwei Cao, Ruo Yuan, Haijun Wang
Summary: In this study, lamellar nanostructures (LNs) of [-TPE-(COOH)4-Al-]nLNs were synthesized through the coordination between Al3+ and carboxyl groups of TPE-(COOH)4. The obtained [-TPE-(COOH)4-Al-]nLNs showed excellent aggregation-induced electrochemiluminescence (AIECL) and higher electrochemiluminescence (ECL) efficiency compared to TPE-(COOH)4. They also demonstrated better cost and biocompatibility compared to coordination aggregation structures with heavy metal or rare metal ions. Furthermore, the [-TPE-(COOH)4-Al-]nLNs were used as a luminescent material to fabricate an ultrasensitive ECL biosensor for miRNA-141 detection using the cascaded strand displacement amplification (CSDA) strategy. The biosensor exhibited excellent detection performance with a wide linear range and low detection limit. This study provides a convenient and effective AIECL strategy for TPE derivatives and expands the application of ECL technology in ultra-sensitive biochemical detection.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Analytical
Yang Yang, Jia-Ling Zhang, Wen-Bin Liang, Jin-Ling Zhang, Xiao-Li Xu, Yong-Jiang Zhang, Ruo Yuan, Dong-Rong Xiao
Summary: A conductive bimetallic MOF nanorod (NiCo-HHTP) with excellent electrical conductivity and ECL performance was synthesized using a doping strategy, which could be utilized for constructing ultrasensitive ECL biosensors.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Danyang Zhang, Manping Qian, Xiaolin Yang, Chengxiao Zhang, Hetong Qi, Honglan Qi
Summary: A label-free electrogenerated chemiluminescence (ECL) aptasensing method was developed for highly sensitive determination of dopamine (DA) based on target-induced DNA conformational change. The method utilizes the hybridization of an anti-DA specific aptamer with a capture ss-DNA to form a self-assembled ds-DNA on a gold electrode, which acts as an ECL biosensing platform. The presence of DA induces DNA conformational change, resulting in the release of intercalated Ru(phen)32+ and a decrease in ECL intensity. By combining specific recognition and amplification with ds-DNA, this method provides a promising strategy for simple, sensitive, and selective DA assay.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Weijia Sun, Nuo Zhang, Xiang Ren, Dan Wu, Yue Jia, Qin Wei, Huangxian Ju
Summary: This study successfully prepared a biosensor for detecting miRNA-21, a potential biomarker for breast cancer. The biosensor coated the electrode with a mesoporous silica encapsulated self-enhanced emitter, enabling sensitive detection of miRNA-21. The method demonstrated good accuracy and reproducibility, providing a new approach for ultra-sensitive detection of miRNA and promoting breast cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Zhuoxin Ye, Yibing Liu, Meichen Pan, Xiuli Tao, Yuxuan Chen, Pinyi Ma, Ying Zhuo, Daqian Song
Summary: In this study, low-toxic quaternary AgInZnS quantum dots were synthesized and used as novel anodic electrochemiluminescence (ECL) emitters. These QDs exhibited strong and stable ECL emission, a low excitation potential, and high ECL efficiency. An on-off-on ECL biosensor for detecting microRNA-141 was designed based on a dual isothermal enzyme-free strand displacement reaction, achieving cyclic amplification of the target and ECL signal, as well as a switch function. The ECL biosensor showed a wide linear range and low detection limit, making it a promising tool for rapid and accurate diagnosis of clinical diseases.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Yuhang Chen, Lingqiu Xu, Qin Xu, Yuqin Wu, Jing Li, Hongbo Li
Summary: DNA nanotechnology is widely used in various fields, and entropy-driven DNA nanomachines have advantages in biosensors. However, the atomic economy of non-covalent molecular reactions in these machines is low, resulting in DNA waste. This study presents a smart photoelectrochemical biosensor design that efficiently avoids waste and achieves high specificity and sensitivity through the use of engineered waste-free DNA amplifiers and superparamagnetic particles.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Yong-ping Gao, Ke-jing Huang, Fu-ting Wang, Yang-yang Hou, Lu-di Zhao, Bo-ya Wang, Jing Xu, Honglei Shuai, Guoqiang Li
Summary: A self-powered biosensor (SPB) utilizing a capacitor/enzymatic biofuel cell (EBFC), rolling circle amplification (RCA), and a digital multimeter (DMM) was developed for ultra-sensitive detection of miR-155. The EBFC-SPB exhibited a sensitivity of 15.85 mu A/pM with the matching capacitor, which is 513% higher than without capacitor (3.09 mu A/pM), achieving the first signal amplification. RCA triggered by miR-155 generated continuous electrons that flowed to the biocathode, catalyzing oxygen reduction and achieving the second signal amplification. DMM was used for signal conversion and real-time amplification, resulting in a LOD of 0.17 fM and a linear range of 0.5 fM to 10,000 fM, indicating the broad application prospects of EBFC-SPB for ultra-sensitive detection of miR-155.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Shuzhen Gao, Jia-Ling Zhang, Jin-Ling Zhang, Mei-Ling Lu, Wen-Bin Liang, Ruo Yuan, Dong-Rong Xiao
Summary: Polycyclic aromatic hydrocarbons (PAHs) are limited in their electrochemiluminescence (ECL) performance in water due to the aggregation-induced quenching (ACQ) effect. To overcome this, PAH derivative ligands were assembled into metal-organic framework nanosheets (MONs) to separate ACQphores and enhance ECL performance. The Hf-DEADB MON prepared using this strategy showed a 5.71-fold improvement in ECL intensity compared to H2DEADB aggregates. The enlarged distance between ACQphores and the ultrathin porous Hf-DEADB MON shortened diffusion pathways, boosting electrochemical activation and enabling the fabrication of a hypersensitive biosensor for microRNA-21 detection with a broad response range and ultralow detection limit.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Liangzhen Xu, Shuang Hou, Xiaocui Huang, Mengyuan Wang, Cheng Li, Nuo Dong, Zhenyu Lin
Summary: A homogeneous electrochemiluminescence (ECL) biosensor based on catalyze hairpin assembly (CHA) and hybridization chain reaction (HCR) and magnetic assisted enrichment has been developed for miR-155 detection in dry eye diseases caused by Sjogren's syndrome. The biosensor offers a simple and sensitive detection method and has been successfully applied in clinical samples.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Linlei Liu, Yuanqi Xu, Yidan Zhu, Haijun Wang, Yaqin Chai, Ruo Yuan
Summary: In this study, carbon nitride quantum dots (CNQDs) with efficient near-infrared electrochemiluminescence (NIR-ECL) and excellent biocompatibility were prepared as an emitter to construct an ultrasensitive regenerative biosensing platform for detection of microRNA-222 (miRNA-222). The introduction of electron-rich pyrimidine effectively increased the conjugation effect of electrons on triazine ring in CNQDs, resulting in red shift of ECL emission wavelength. An improved circular DNA walker was assembled by the catalytic ligation of T4 DNA ligase to enhance its stability and walking efficiency. By utilizing CNQDs as emitter and circular DNA walker as signal amplifier, a regenerative biosensor platform was developed for ultrasensitive detection of miRNA-222 with high sensitivity and successfully applied for detection in cancer cells lysate. This work opens up a promising approach for synthesizing efficient NIR-ECL CNQDs, reducing damage to organisms and achieving deeper tissue penetration, and provides a new method for biomarker detection and disease diagnosis.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemical Research Methods
Yanjie Zheng, Jinyuan Chen, You Li, Yichun Xu, Li Chen, Wei Chen, Ailin Liu, Xinhua Lin, Shaohuang Weng
Summary: This study developed a method for simultaneous detection of miRNA-21 and miRNA-155 using T7 exonuclease-assisted target recycling amplification and graphene oxide fluorescence quenching, showing high sensitivity and excellent analytical performance. The method has the potential to effectively warn drug resistance in lung cancer through monitoring abnormal miRNA expression.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Liang Tian, Jiyuan Zhang, Yanci Zhang, Huiling Fan, Chun Liu, Yihong Wang, Jiansheng Cui
Summary: A dual-model photoelectrochemical biosensor based on label-free trimetallic nanoprobe and Mg2+ dependent DNAzyme driven DNA walker was developed for ultrasensitive detection of microRNA-155 in serum at the early stage of cancer. The biosensor exhibited a low limit of detection (1.2 fM), excellent stability, and superior performance in complex human serum samples and tumor cell lysates, showing great potential for early-stage clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)