4.8 Article

3D printed microfluidic devices for circulating tumor cells (CTCs) isolation

期刊

BIOSENSORS & BIOELECTRONICS
卷 150, 期 -, 页码 -

出版社

ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.111900

关键词

3D printing; Circulating tumor cell (CTC); Microfluidic separation; Immunocapture; Diagnostics

资金

  1. Hangzhou Wahaha Group Co., Ltd. (China)

向作者/读者索取更多资源

Isolation of circulating tumor cells (CTCs) from blood samples has important prognostic and therapeutic implications for cancer treatments, but the process is very challenging due to the low concentration of CTCs. In this study, we report a novel 3D printed microfluidic device functionalized with anti-EpCAM (epithelial cell adhesion molecule) antibodies to isolate CTCs from human blood samples. A 3D printing technology was utilized with specially designed interior structures to fabricate a microfluidic device with high surface area and fluid flow manipulation, increasing capture efficiency of tumor cells. These devices with the optimal flow rate (1 mL/h) and channel length (2 cm) were demonstrated to test three kinds of EpCAM positive cancer cell lines (MCF-7 breast cancer, SW480 colon cancer, and PC3 prostate cancer), and one kind of EpCAM negative cancer cell line (293T kidney cancer). Experimentally, the capture efficiency higher than 90% has been achieved, and the isolation of MCF-7 tumor cells from spiked human blood samples has also been demonstrated. Combined with DNA-based detection (e.g. polymerase chain reaction or DNA sequencing), the detection and analysis of released DNAs from captured tumor cells could be another future direction for clinical diagnosis and cancer treatment.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

Article Chemistry, Organic

On Topological Properties of Planar Octahedron Networks

Jia-Bao Liu, Haidar Ali, Muhammad Kashif Shafiq, Ghulam Dustigeer, Parvez Ali

Summary: The research focuses on the applications of topological indices in analyzing the bio-compatibility of chemical compounds in the context of QSAR and QSPR correlations. By combining Chemistry and Graph theory, Chemical graph theory deals with topology and plays a significant role in the development of topological indices. The article specifically explores the use of degree based topological indices in Octahedron networks.

POLYCYCLIC AROMATIC COMPOUNDS (2023)

Article Substance Abuse

Applying topic modelling and qualitative content analysis to identify and characterise ENDS product promotion and sales on Instagram

Neal Shah, Matthew Nali, Cortni Bardier, Jiawei Li, James Maroulis, Raphael Cuomo, Tim K. Mackey

Summary: This study used unsupervised machine learning to analyze the sales of electronic nicotine delivery systems (ENDS) on Instagram. The results showed that there is a significant amount of unregulated ENDS sales on Instagram, including flavored products, and many websites lack age verification. These findings may violate Instagram's policies and US laws, highlighting the need for stronger regulation to prevent youth access to and use of ENDS.

TOBACCO CONTROL (2023)

Article Computer Science, Artificial Intelligence

Aerial Images Meet Crowdsourced Trajectories: A New Approach to Robust Road Extraction

Lingbo Liu, Zewei Yang, Guanbin Li, Kuo Wang, Tianshui Chen, Liang Lin

Summary: This paper discusses an important task in land remote-sensing analysis, which is the automatic extraction of traffic roads from remote-sensing data. To address this problem, the authors introduce a novel neural network framework that utilizes the complementary information from different data sources to enhance the accuracy and robustness of road extraction.

IEEE TRANSACTIONS ON NEURAL NETWORKS AND LEARNING SYSTEMS (2023)

Article Biochemical Research Methods

A strategy to explore the quality markers of Ziziphi Spinosae semen by combining metabolic in vivo study with network pharmacology

Yan Yan, Jiaxing Liu, Min Zhang, Yinjie Zhang, Biyun Shi, Xuemei Qin, Chenhui Du

Summary: In this study, quality markers for Ziziphi Spinosae semen (ZSS) were proposed using a combination of metabolic in vivo study and network pharmacology. An UHPLC-Q-Orbitrap-MS method was used to identify and assign components in ZSS, and metabolic networks of flavonoids, saponins, and alkaloids were established. Twelve key bioactive components against insomnia were selected as quality markers for ZSS.

BIOMEDICAL CHROMATOGRAPHY (2023)

Article Cell Biology

HELQ suppresses migration and proliferation of non-small cell lung cancer cells by repairing DNA damage and inducing necrosis

Nan Shan Zhong, Wei Lai Tong, Yu Zhang, Shi Ning Xiao, Jia Ming Liu, An An Li, Ge Liang Yao, Qing Lin, Zhi Li Liu

Summary: HELQ plays a tumor-suppressive role in non-small-cell lung cancer and has the potential to be a predictive biomarker for prognosis and treatment of lung cancer.

CELL BIOLOGY INTERNATIONAL (2023)

Article Materials Science, Paper & Wood

Fabrication of durable coatings for cotton fabrics with flame retardant, antibacterial, Fluorine-free Superhydrophobic and self-cleaning properties

Jianjun Gu, Xiaofei Yan, Dongming Qi, Ruyi Xie, Xiaoming Yang, Yaobang Li, Jiawei Li

Summary: In this study, a durable multifunctional coating was successfully fabricated on cotton fabric, providing it with flame retardant, antibacterial, superhydrophobic and self-cleaning properties. The coated fabric exhibited excellent durability even after multiple washing cycles.

CELLULOSE (2023)

Article Gastroenterology & Hepatology

Application of endoscopic purse-string sutures in high-risk peptic ulcer hemorrhage: preliminary experience of 38 cases

Jingxian Hu, Miao Jiang, Hongyan Liu, Hao Zhou, Yajie Wang

Summary: The study finds that endoscopic purse-string sutures appear to be safe and effective in the treatment of high-risk peptic ulcer hemorrhage patients.

SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY (2023)

Review Materials Science, Textiles

Simulation of fiber arrangement in slub strands and twisting on the slub yarn with the finite element method

Shifeng Wu, Jiawei Li, Chongwen Yu

Summary: This paper proposes a simulation method for the fiber arrangement in slub yarn and uses the finite element method to simulate the twist distribution of slub yarn, which is consistent with actual spinning results. The research is significant for further exploration of the properties of slub yarn.

TEXTILE RESEARCH JOURNAL (2023)

Review Chemistry, Multidisciplinary

Progress in the Foaming of Polymer-based Electromagnetic Interference Shielding Composites by Supercritical CO2

Jianming Yang, Xin Yan, Xinru Xu, Yujian Chen, Wei Han, Xianzhi Chai, Xiang Liu, Jiaxing Liu, Chen Liu, Hexin Zhang, Xiao Li, Zhen Zhang, Tie Wang

Summary: Polymeric electromagnetic interference (EMI) shielding materials based on CO2 foaming technology have gained widespread attention due to their efficient use of CO2, high strength, corrosion resistance, and low cost. New design concepts and preparation techniques have led to the development of high-performance EMI shielding materials. This review summarizes the progress made in fabricating EMI shielding composite foams using supercritical carbon dioxide (scCO(2)) foaming and explores the relationship between component/distribution and EMI shielding properties. Additionally, it discusses the application prospects and development challenges of new EMI shielding composite foams.

CHEMISTRY-AN ASIAN JOURNAL (2023)

Article Computer Science, Interdisciplinary Applications

Sequential fully implicit newton method for flow and transport with natural black-oil formulation

Jiawei Li, Pavel Tomin, Hamdi Tchelepi

Summary: There is an increasing interest in developing robust and efficient sequential methods for reservoir simulation. The sequential fully implicit Newton (SFIN) method addresses the slow sequential coupling convergence issue when flow and transport problems are strongly coupled. However, the original SFIN algorithm requires fixed primary variables during the simulation. In this work, strategies are proposed to handle inconsistent primary variables and extend the SFIN method to the natural black-oil formulation.

COMPUTATIONAL GEOSCIENCES (2023)

Article Dentistry, Oral Surgery & Medicine

Impaired autophagy flux by lncRNA NEAT1 is critical for inflammation factors production in human periodontal ligament stem cells with nicotine treatment

Taotao Zhang, Kuan Yang, Yujiang Chen, Yuran Jiang, Zhifei Zhou, Jiajia Liu, Yang Du, Lulu Wang, Xinxin Han, Xingan Wu, Xiaojing Wang

Summary: This study found that nicotine treatment impairs autophagy function in periodontal ligament stem cells, leading to the production of inflammatory factors. Activation of α7nAChR by nicotine upregulates NEAT1, which in turn decreases autophagy flux by downregulating STX17.

JOURNAL OF PERIODONTAL RESEARCH (2023)

Review Chemistry, Multidisciplinary

Flexible Electronics Based on Organic Semiconductors: from Patterned Assembly to Integrated Applications

Haoran Liu, Dong Liu, Junchuan Yang, Hanfei Gao, Yuchen Wu

Summary: Organic flexible electronic devices with outstanding properties, such as solution processability, lightweight, and flexibility, have the potential to revolutionize lifestyles. The precise patterning and ordered assembly of organic semiconductors for integration in flexible electronics have attracted attention and rapidly developed. This review focuses on recent advances in the design, patterned assembly, and multifunctional applications of flexible organic field-effect transistors (FOFETs). It introduces typical organic semiconductor materials and material design methods, discusses patterned assembly strategies on flexible substrates, and highlights advanced applications of flexible electronic devices based on organic semiconductor patterns. Future challenges and possible directions for the development of the next generation of flexible electronics are also proposed.
Article Spectroscopy

Theoretical perspective for substitution effect on luminescent properties of through space charge transfer-based thermally activated delayed fluorescence molecules

Haipei Zou, Huanling Liu, Qingfang Mu, Kai Zhang, Yuzhi Song, Lili Lin, Yuanyuan Xu, Chuan-Kui Wang, Jianzhong Fan

Summary: This study investigates the relationship between molecular structures and luminescent properties, and proposes theoretically efficient TSCT-based TADF molecules. These molecules have a large number of substitutions and adopt twist structures to achieve small energy gaps between the lowest excited states.

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY (2023)

Article Endocrinology & Metabolism

Identification of Risk Factors and Development of a Predictive Model for Postoperative Hypoglycemia among Diabetic Patients during the Perioperative Period

Zixuan Liu, Jing Dai, Xiaodie He, Jiaxi Li, Haixia Zhang, Cheng Ji

Summary: This study aimed to investigate the incidence and influencing factors of postoperative hypoglycemia in diabetic patients during the perioperative period and construct a risk prediction model. Patients with type 2 diabetes admitted to Nanjing Drum Tower Hospital from December 2019 to January 2022 were included. A total of 440 patients were included, and the incidence of postoperative hypoglycemia was 24.78%. Preoperative C-peptide and operation duration were identified as risk factors, while BMI and preoperative fasting blood glucose were protective factors.

INTERNATIONAL JOURNAL OF ENDOCRINOLOGY (2023)

Article Computer Science, Artificial Intelligence

Integrity verification for scientific papers: The first exploration of the text

Xiang Shi, Yinpeng Liu, Jiawei Liu, Qikai Cheng, Wei Lu

Summary: Scientific papers are crucial for academic communication, but many of them lack in-depth research and present core content ambiguously, hindering the progression of science and technology. To address this challenge, the INTEGrity vERification (INTEGER) task is introduced to help researchers assess the integrity of their papers and verify the clarity of each knowledge unit. A multi-task learning model utilizing Tucker decomposition and span-level attention mechanism is proposed to accurately identify terms and their integrity. Experimental results show the effectiveness of the model.

EXPERT SYSTEMS WITH APPLICATIONS (2024)

Article Biophysics

An integrated centrifugal microfluidic strategy for point-of-care complete blood counting

Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari

Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Immunity testing against COVID-19 from blood by an IoT-enabled and AI-controlled multiplexed microfluidic platform

Nabil H. Bhuiyan, Joon S. Shim

Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Influence of shear stress on electroactive biofilm characteristics and performance in microbial fuel cells

Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour

Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Cheap and portable paper chip with terrific oxidase-like activity and SERS enhancement performance for SERS-colorimetric bimodal detection of intracellular glutathione

Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li

Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Tracking the EMT-like phenotype switching during targeted therapy in melanoma by analyzing extracellular vesicle phenotypes

Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau

Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.

BIOSENSORS & BIOELECTRONICS (2024)

Review Biophysics

Protease detection in the biosensor era: A review

Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov

Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Controllable self-assembled DNA nanomachine enable homogeneous rapid electrochemical one-pot assay of lung cancer circulating tumor cells

Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin

Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Therapeutic drug monitoring mediated by the cooperative chemical and electromagnetic effects of Ti3C2TX modified with Ag nanocubes

Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang

Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

CRISPR-Cas12a powered hybrid nanoparticle for extracellular vesicle aggregation and in-situ microRNA detection

Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu

Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Facile synthesis of dual-ligand europium-metal organic gels for ratiometric electrochemiluminescence detecting I27L gene

Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li

Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

The use of biological fluids in microfluidic paper-based analytical devices (μPADs): Recent advances, challenges and future perspectives

Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho

Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Development of a peptide microarray-based metal-enhanced fluorescence assay for ultrasensitive detection of multiple matrix metalloproteinase activities by using a gold nanorod-polymer substrate

Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang

Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Plasmonic digital PCR for discriminative detection of SARS-CoV-2 variants

Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon

Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

A wearable and flexible lactic-acid/O2 biofuel cell with an enhanced air-breathing biocathode

Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu

Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Gas-responsive two-dimensional metal-organic framework composites for trace visualization of volatile organic compounds

Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang

Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.

BIOSENSORS & BIOELECTRONICS (2024)