期刊
BIOSENSORS & BIOELECTRONICS
卷 142, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.111521
关键词
Electrochemiluminescence; Self-enhanced; Ru(bpy)(3)(2+); Mesoporous MOF; Double quenching; Polydopamine; Procalcitonin
类别
资金
- National Key Scientific Instrument and Equipment Development Project of China [21627809]
- National Natural Science Foundation of China [21505051, 21575050, 21607055, 21777056]
- Jinan Scientific Research Leader Workshop Project [2018GXRC024]
The rapid and reliable measurement of procalcitonin (PCT) level exerts a prominent impact on controlling infections and preventing septicemia. This work presents a signal on-off electrochemiluminescence (ECL) immunosensor for ultrasensitive PCT detection based on the double quenching effects of Fe3O4@PDA-CuxO towards a self-enhanced Ru (bpy)(3)(2+)-functionalized metal organic framework (MOF). Specifically, mesoporous and hollow MIL-101(Al)-NH2 is first applied in ECL system to load Ru (bpy)(3)(2+) abundantly and stably. Poly (ethylenimine), as the coreactant, is covalently linked with MIL-101(Al)-NH2 to prevent the leakage of Ru (bpy)(3)(2+) and form a self-enhanced Ru complex, thus strong and stable ECL signals are obtained initially. To achieve high-efficiency quenching of signal on state, Fe3O4@PDA-CuxO-Ab(2) is synthesized tactfully as the quenching probe by which catechol or benzoquinone units in PDA and Cu2+ adsorbed by PDA quench the excited states of Ru (bpy)(3)(2+) via electron transfer and resonance energy transfer. In this way, a rapid and robust signal-quenched type ECL immunosensor is developed for PCT measurement with a detection limit of 0.18 pg(-1) and a dynamic range from 0.0005 ng mL(-1) to 100 ng mL(-1). Given desirable selectivity, stability and reproducibility, the elaborated ECL immunoassay based on luminescence-functionalized MOF prefigures its great promise in bioanalysis of PCT and other biomarkers.
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