期刊
BIORESOURCE TECHNOLOGY
卷 297, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2019.122471
关键词
Tobacco stalk; Pretreatment; Alkali cooking; Enzymatic hydrolysis; Hemicelluloses
资金
- National Key RAMP
- D Program of China [2017YFB0307903]
- National Natural Science Foundation of China [31430092]
- Fundamental Research Funds for the Central Universities [2015ZCQ-CL-02]
Hereon, tobacco stalk was deconstructed by lyophilization, ball-milling, ultrasound-assisted alkali extraction, hydrothermal pretreatment (HTP), and alkali presoaking, respectively, followed by dilute alkali cooking to both improve its enzymatic digestibility and isolate the hemicellulosic streams. It was found that a maximum cellulose saccharification rate of 93.5% was achieved from the integrated substrate by ball-milling and dilute alkali cooking, which was 4.4-fold higher than that from the raw material. Interestingly, in this case, 76.9% of hemicelluloses were simultaneously recovered during the integrated treatment. Structural determination indicated that the hemicelluloses released from tobacco stalk by dilute alkali cooking were mixed polysaccharides, and the (1 -> 4)-linked beta-D-Xylp backbone branched with L-Araf units at O-2/O-3 and 4-O-Me-alpha-D-GlcpA units at O-2 of the xylose residues was the main structure. In comparison, ultrasound-assisted alkali extraction, ballmilling, and HTP favored the extraction of hemicelluloses with less branched structure and lower molecular weights in the following alkali cooking.
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