Article
Chemistry, Applied
Min Wang, Jianjun Zhou, Juan Manuel Castagnini, Houda Berrada, Francisco J. Barba
Summary: This study investigated the effects of pulsed electric field, solvent, and time on the extraction of Chlorella antioxidant bio-molecules and minerals. The results showed that PEF treatment increased the biomolecules recovery, and using water as the extraction solvent for 120 minutes obtained more proteins and polyphenols, while using 50% DMSO obtained more chlorophyll a and b, and carotenoids.
Article
Energy & Fuels
Ankesh Ahirwar, Mohd Jahir Khan, Vandana Sirotiya, Megha Mourya, Anshuman Rai, Benoit Schoefs, Justine Marchand, Sunita Varjani, Vandana Vinayak
Summary: Haematococcus pluvialis is a potential source of natural astaxanthin with remarkable antioxidant properties. The high cost of extracting this pigment makes the cost of astaxanthin very high. Pulsed electric field (PEF) has been found to be a simple yet sensitive technique to permeabilize the thick cell wall of H. pluvialis and extract the pigment.
BIOENERGY RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Ralf Straessner, Marcell Nikolausz, Aude Silve, Natalja Nazarova, Ruediger Wuestner, Ioannis Papachristou, Sahar Akaberi, Klaus Leber, Georg Mueller, Wolfgang Frey
Summary: This study proposes a cascade processing method for utilizing lipid-rich microalgae Auxenochlorella protothecoides. The method includes pre-concentration of biomass, pretreatment using pulsed electric fields (PEF), separation of aqueous fraction for recovery of valuable components, and lipid extraction from the remaining wet pellet. The remaining biomass is then subjected to anaerobic digestion (AD) for biogas production. The study demonstrates the feasibility and potential of this approach for industrial implementation.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Chemistry, Multidisciplinary
Carlota Delso, Aude Silve, Ruediger Wuestner, Natalja Nazarova, Ignacio Alvarez, Javier Raso, Wolfgang Frey
Summary: This study focuses on under-investigated processing parameters of pulsed electric field (PEF) treatment for efficient lipid extraction from microalgae, such as media pH, pulse application, and incubation protocols. The results show that medium pH is critical in the final electroporation and lipid extraction yields, and should be considered in further studies.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Food Science & Technology
Tong-Ge Bai, Liang Zhang, Jian-Ya Qian, Wei Jiang, Mangang Wu, Sheng-Qi Rao, Qian Li, Chen Zhang, Chunsen Wu
Summary: PEF pretreatment has significant impacts on the properties of rice, including increased starch hydrolysis degree and hardness, decreased integrity of cell structure, and altered chemical composition of cooked rice.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Si-Yeon Kim, Byung-Min Lee, Seok-Young Hong, Hyun-Ho Yeo, Se-Ho Jeong, Dong-Un Lee
Summary: This study investigated the effects of pulsed electric field (PEF) treatment on the drying and rehydration kinetics, microstructure, and carotenoid content of carrot. The results showed that PEF treatment reduced drying time, improved rehydration ability, and preserved carotenoid content.
Article
Food Science & Technology
Se-Ho Jeong, Young-Min Jung, Siyeon Kim, Jong-Hun Kim, Hyunho Yeo, Dong-Un Lee
Summary: This study investigated the effects of pulse electric field (PEF) treatment on the tenderization of beef semitendinosus muscle. An adjustable PEF chamber was designed to directly contact the beef sample without water as the transmitting medium. PEF treatment induced structural changes in beef muscle, and the degree of changes depended on the electric field strength. At 2.0 kV/cm, cutting force, hardness, and chewiness significantly decreased, and troponin-T was extensively degraded. Under a certain intensity (1.0 kV/cm), PEF treatment retained the fresh quality factors such as color and lipid oxidation.
Article
Polymer Science
Ahmed Taha, Federico Casanova, Martynas Talaikis, Voitech Stankevic, Nerija Zurauskiene, Povilas Simonis, Vidas Pakstas, Marijus Jurkunas, Mohamed A. E. Gomaa, Arunas Stirke
Summary: Pulsed electric field (PEF) as a green processing technology can change the structure and physicochemical properties of casein micelles (CSMs), enhancing their techno-functional properties. The effects of different electric field strengths on CSMs were investigated, showing that particle size, surface hydrophobicity, and protein solubility were influenced by PEF treatment. Moreover, PEF treatment affected the fluorescence spectrum and secondary structure of CSMs, as well as disulfide linkages. Overall, PEF technology has the potential to improve CSMs' properties and contribute to sustainable development goals.
Article
Food Science & Technology
Greta Canelli, Isabelle Kuster, Luc Jaquenod, Leandro Buchmann, Patricia Murciano Martinez, Zhen Rohfritsch, Fabiola Dionisi, Christoph J. Bolten, Paolo Nanni, Alexander Mathys
Summary: The study demonstrated that pulsed electric field treatment can enhance lipid bioaccessibility of Chlorella vulgaris while maintaining cell integrity and oxidative stability, requiring incubation post-treatment. Proteome analysis identified four proteins that may be involved in cell wall lytic activity during incubation after PEF. Future research should focus on further understanding the mechanism behind incubation after PEF and the potential effect played by endogenous cell wall-degrading enzymes.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2022)
Article
Food Science & Technology
Tomas Bolumar, Bo-Anne Rohlik, Janet Stark, Anita Sikes, Peter Watkins, Roman Buckow
Summary: This study investigated the pulsed electric field (PEF) conditions for tenderizing beef topside. The results showed that PEF treatment did not affect meat tenderness after 1 day of storage, but resulted in a reduction in shear force after 14 days of storage. Other quality traits were not impaired. PEF technology is a possible intervention to improve meat tenderness after 2 weeks of storage.
Article
Food Science & Technology
Dagmar Ammelt, Alica Lammerskitten, Artur Wiktor, Francablisco J. Barba, Stefan Toepfl, Oleksii Parniakov
Summary: The study investigated the impact of pulsed electric fields (PEF) on the cell disintegration index (Zp), freeze-drying process, and final product quality of red beet and pineapple tissue. While it did not reduce drying time, PEF pre-treatment significantly enhanced the quality of the freeze-dried product. The effectiveness of PEF was found to be dependent on the type of plant material used.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Nutrition & Dietetics
Serena Carpentieri, Anet Rezek Jambrak, Giovanna Ferrari, Gianpiero Pataro
Summary: The effect of pulsed electric field (PEF) pre-treatment on the extractability of target aroma and bioactive compounds in green solvents was investigated. PEF treatment significantly enhanced the extraction yield of the target compounds in both solvents, indicating potential for sustainable recovery of natural compounds.
FRONTIERS IN NUTRITION
(2022)
Article
Food Science & Technology
Jianjun Zhou, Min Wang, Houda Berrada, Zhenzhou Zhu, Nabil Grimi, Francisco J. Barba
Summary: This study evaluates the impact of different processes (PEF, PLE, and PEF + PLE) on the yield of antioxidant compounds from Spirulina. The results show that PEF + PLE significantly improves the extraction yield and increases the protein, polyphenol, chlorophyll a, and antioxidant capacity values of Spirulina extracts.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2022)
Article
Environmental Sciences
Ozlem Selcuk Kuscu, Ekin EKE
Summary: In this study, nutrient recovery from sewage sludge as struvite using pulsed electric field technique was successfully conducted. The best struvite formation conditions were determined using response surface methodology, with high agreement between predicted and experimental results. Pre-treatment with PEF resulted in a significant decrease in heavy metal content in the sludge.
WATER AIR AND SOIL POLLUTION
(2021)
Article
Food Science & Technology
Chen Zhang, Ya-Hui Yang, Xu-Dong Zhao, Liang Zhang, Qian Li, Chunsen Wu, Xiangli Ding, Jian-Ya Qian
Summary: The study demonstrated that PEF treatment had significant effects on the functional, rheological, and structural properties of vital wheat gluten, impacting parameters such as solubility, waterholding capacity, oil-holding capacity, foaming capacity, and more. Additionally, the increase in electric field intensity resulted in changes in the viscoelasticity and stability of the gluten protein.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Plant Sciences
Olivia Spain, Martin Plohn, Christiane Funk
Summary: Heavy metals in industrial wastewaters pose a serious threat to the environment and human health. Microalgae are increasingly being recognized as potential solutions for removing pollutants through biosorption due to their unique cell wall structure. The review focuses on the composition and structure of microalgae cell walls in heavy metal removal, as well as commonly used models to predict the velocity and capacity of biosorption by microalgae.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Food Science & Technology
L. Astrain-Redin, J. Moya, M. Alejandre, E. Beitia, J. Raso, B. Calvo, G. Cebrian, I Alvarez
Summary: The study showed that regulating the electrode temperature can reduce temperature differences, increase the uniformity of heat treatment, and improve microbial inactivation effectiveness.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Carlota Delso, Alejandro Berzosa, Jorge Sanz, Ignacio Alvarez, Javier Raso
Summary: The aim of this study was to evaluate the potential of Pulsed Electric Fields (PEF) technology as an alternative method for processing red grape juice. The results showed that PEF-treated grape juice had higher polyphenol content and color intensity compared to control juices. The subsequent PEF decontamination treatment achieved complete microbial inactivation and the PEF-treated juice remained microbiologically stable for up to 45 days. The quality and sensory characteristics of the PEF-treated juice were not affected by the decontamination treatment or storage time and temperature.
Article
Environmental Sciences
Martin Plohn, Kim Scherer, Stefan Stagge, Leif J. Jonsson, Christiane Funk
Summary: Microalgae have potential as candidates for sustainable wastewater treatment and the production of biofuels, bioplastics, and bio-fertilizers. In Nordic countries, where light is limited during the winter season, the utilization of carbon sources from industrial wastewater streams, particularly those from the pulp and paper industry, could enhance biomass production. This study focused on the utilization of methanol, glycerol, and xylose by five different Nordic microalgae strains. Two of these strains were able to grow in the presence of xylose or methanol, while glycerol increased growth for one strain. These microalgae strains show potential for the production of biofuels and further optimization of cultivation conditions is required.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Olivia Spain, Christiane Funk
Summary: This study investigated the cell wall compositions and morphologies of four Nordic microalgal strains, revealing significant differences in sugar and protein composition of the Scenedesmus sp. (B2-2) cell wall compared to the other three strains. Microscopy, spectroscopy, and derivatization techniques were employed to analyze the cell walls.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agricultural Engineering
Sanjeet Mehariya, Martin Plohn, Antonio Leon-Vaz, Alok Patel, Christiane Funk
Summary: Nordic Desmodesmus microalgal strains showed increased lipid and carotenoid production under abiotic stress conditions of light and salt. The biomass output of these strains also significantly improved under stress, indicating their potential for use in low-cost algal bio-factories.
BIORESOURCE TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Vida Strancar, Katarina P. van Midden, Daniel Krahn, Kyoko Morimoto, Marko Novinec, Christiane Funk, Simon Stael, Christopher J. Schofield, Marina Klemencic, Renier A. L. van der Hoorn
Summary: This study developed activity-based probes for the three main types of Metacaspases and demonstrated the essential role of calcium in the activation process. The findings suggest the formation of active, unprocessed intermediates upon calcium binding before precursor protein maturation.
Article
Environmental Sciences
Antonio Leon-Vaz, Juan Cubero-Cardoso, Angeles Trujillo-Reyes, Fernando G. Fermoso, Rosa Leon, Christiane Funk, Javier Vigara, Juan Urbano
Summary: In recent years, there has been growing concern over the pollution of aqueous ecosystems by heavy metals, prompting the need for better remediation techniques. This study explores the combination of a copolymer synthesized from sulfur and vegetable oils and the microalga Chlorella sorokiniana to remove heavy metals from water. Results show that the AlgaPol biofilm is capable of removing over 90% of these metals from the growth medium. The excellent metal adsorption capacity of the biofilm can be described by a pseudo-second-order kinetic model.
Article
Food Science & Technology
Vanesa Abad, Marta Alejandre, Elena Hernandez-Fernandez, Javier Raso, Guillermo Cebrian, Ignacio Alvarez-Lanzarote
Summary: Larvae of the Anisakidae family can cause parasitic infections in humans through fish consumption. Methods for inactivating and eliminating these larvae need to be established, especially for raw or insufficiently treated fish products. This study evaluates the efficacy of pulsed electric fields (PEF) in inactivating Anisakis larvae and shows that PEF can be an alternative to traditional freezing processes for parasite inactivation.
Article
Agricultural Engineering
Sanjeet Mehariya, Martin Plohm, Piotr Jablonski, Stefan Stagge, Leif J. Jonsson, Christiane Funk
Summary: This study explores the use of biomass from four different Nordic microalgal species as a carbohydrate-rich feedstock for polyhydroxybutyrate (PHB) production. Thermochemical pre-treatment with hydrochloric acid or sulphuric acid significantly increased the sugar yield. The sugar-rich hydrolysate was used as a carbon source for PHB production by Halomonas halophila, and the highest PHB production was achieved with hydrolysate from Chlorococcum sp. grown on BG-11 medium.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biochemical Research Methods
Antonio Leon-Vaz, Rosa Leon, Javier Vigara, Christiane Funk
Summary: Nordic microalgae are photosynthetic organisms adapted to low temperature and varying light conditions. They have the potential for large-scale cultivation and production of high-value compounds due to their ability to withstand harsh environments. Some Nordic microalgae species have been found to produce high amounts of carotenoids and polyphenols, and exhibit improved performance under high light and cold stress conditions. Therefore, their cultivation at large scale in Nordic environments is justified.
Article
Multidisciplinary Sciences
Teodor Alling, Christiane Funk, Francesco G. Gentili
Summary: Microalgal biomass has biostimulating effects on plants and seeds due to its phytohormonal content, and harnessing this effect could contribute to sustainable agriculture.
SCIENTIFIC REPORTS
(2023)
Article
Food Science & Technology
J. M. Martinez, V. Abad, J. Quilez, J. Raso, G. Cebrian, I. Alvarez-Lanzarote
Summary: The burden of food-borne parasites on public health is underestimated and the relative importance of different transmission routes is not completely known, resulting in their inadequate control in food. Pulsed Electric Field (PEF) technology has the potential to effectively inactivate food-borne parasites without affecting food quality, providing an alternative to traditional freezing processes. Further research and collaborations across diverse fields are needed for the industrial implementation of PEF.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Chemical
Natalia Salgado-Aristizabal, Tatiana Agudelo-Patino, Sebastian Ospina-Corral, Ignacio Alvarez-Lanzarote, Carlos Eduardo Orrego
Summary: A study using GPS tracking on critically endangered Zino's petrels from Madeira, Portugal, has revealed their migration routes. The research found that these birds migrate to the African continent during the warm seasons and return to Madeira during the cold seasons. These findings are significant for the conservation of these endangered birds and understanding their migration behavior.
Article
Food Science & Technology
Florencia P. Alloggia, Roberto F. Bafumo, Daniela A. Ramirez, Marcos A. Maza, Alejandra B. Camargo
Summary: Microgreens are new foods that are easy and sustainable to grow, with high concentrations of bioactive compounds. Brassicaceae microgreens are particularly beneficial for human health due to their phenolic compounds, vitamins, glucosinolates, isothiocyanates, and indoles. The cultivation conditions, such as substrates, artificial lighting, and fertilization, can affect the phytochemical profiles and provide strategies to enhance the functionality and health benefits of Brassicaceae microgreens.
CURRENT RESEARCH IN FOOD SCIENCE
(2023)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)
