期刊
BIORESOURCE TECHNOLOGY
卷 289, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2019.121625
关键词
Microalgal proteins; Chlorella; Antibacterial activity; Prebiotic properties
资金
- Ministry of Science and Technology [MOST 108-2218-E-006-006, MOST 107-2218-E-006-016, MOST 106-3114-E-006-008, MOST 105-2221-E-006-225-MY3, MOST 105-2621-M-006-012-MY3]
In this study, productions of microalgal proteins were explored via a circular economy concept. First, production of proteins from Chlorella vulgaris FSP-E (CV) and Chlorella sorokiniana (CS) was optimized by using favorable cultivation conditions and strategies. The optimal CO2 concentration for the growth of both microalgae was 5% (v/v), while the optimal nitrogen source for CV and CS were 12 mM of NaNO3 and NH4Cl, respectively. Addition of 12 mg/L ammonium iron (III) citrate enhanced protein production. Next, semi-batch cultivation strategy was employed to achieve a protein production of 793.3 and 812.8 mg/L for CV and C S, representing a 4.86 and 2.77 fold increase, respectively, in protein productivity. The obtained microalgal proteins consist of 40% essential amino acids. The CV and CS proteins possess prebiotic activities as they enhanced the growth of Lactobacillus rhamnosus ZY by 48 and 74%, respectively, with a good antibacterial activity against predominant pathogens.
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