Review
Biochemical Research Methods
Catarina M. Abreu, Bruno Costa-Silva, Rui L. Reis, Subhas C. Kundu, David Caballero
Summary: Extracellular vesicles (EVs) play a critical role in cancer and microfluidic platforms provide a promising approach for the isolation, analysis, and quantification of EVs. These platforms offer high-throughput, sensitivity, and multiplexing capabilities, and can be used for EV-based therapies.
Article
Chemistry, Analytical
Wenjie Zhao, Lingqian Zhang, Yifei Ye, Yuang Li, Xiaofeng Luan, Jinlong Liu, Jie Cheng, Yang Zhao, Mingxiao Li, Chengjun Huang
Summary: The study developed a method for isolating and detecting exosomes based on a microfluidic device, achieving successful isolation and quantitative detection of exosomes. By monitoring the protein expression levels in exosomes from different cell lines, the method shows promise for exosome-based tumor marker detection and cancer diagnosis.
Article
Biochemical Research Methods
Yang Bu, Jinhui Wang, Sheng Ni, Yusong Guo, Levent Yobas
Summary: Extracellular vesicles (EVs) are cell-derived bioparticles that have significant roles in various biological processes and can be potential biomarkers for pre-diagnostic applications. However, isolating EV subpopulations, especially exosomes, from biological fluids is challenging due to their small size. In this study, a microfluidic device combining electrothermal fluid rolls and dielectrophoresis was used for continuous-flow label-free size fractionation of EVs. The device showed high efficiency in separating submicron particles and isolating intact exosomes from cell culture medium or blood serum. This method provides a promising platform for the purification of target bioparticles directly from physiological fluids and may have important implications in EV-related diagnostic applications.
Review
Chemistry, Analytical
Junjie Bai, Xing Wei, Xuan Zhang, Chengxin Wu, Zejun Wang, Mingli Chen, Jianhua Wang
Summary: This article discusses the application of microfluidic systems in the precise manipulation and effective separation of exosomes, including immune-affinity-based microchip, size-based micro-device, and multi-approaches integration microfluidic device. The article also introduces the combination of advanced techniques with nano/microfluidic chip for individual exosomes imaging or assay and the isolation of single exosome with microfluidics. In addition, the opportunities and challenges of intelligent algorithms and machine learning in multi-dimensional profiling of individual exosomes are emphasized.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Duraichelvan Raju, Srinivas Bathini, Simona Badilescu, Anirban Ghosh, Muthukumaran Packirisamy
Summary: Extracellular vesicles (EVs) are communication organelles enclosed by a phospholipid bilayer, containing a myriad of compounds from origin cells and serving as a good source of biomarkers. Exosomes, a subset of EVs, hold significant importance in future medicine, but their practical applications are hindered by the lack of efficient isolation and detection techniques.
Article
Chemistry, Multidisciplinary
Delaram Parvin, Zahra Sadat Hashemi, Farhad Shokati, Zahra Mohammadpour, Vahid Bazargan
Summary: By integrating magnetic nanoparticles with microfluidics, an effective and low-cost method for isolating HER2-positive breast cancer cells was developed, which improved the efficiency of cell isolation and reduced the analysis time. This microfluidic system offers a competitive solution for clinical applications.
Article
Biophysics
Aynur Abdulla, Zhianan Zhang, Khan Zara Ahmad, Antony R. Warden, Hengyu Li, Xianting Ding
Summary: This study demonstrates a novel antibody functionalized microfluidic chip that can rapidly and accurately capture circulating tumor cells (CTCs) from cancer patients' whole blood. The chip offers a valuable tool for cancer screening, prognosis, and diagnosis.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Biochemical Research Methods
Long Ngo, Le Que Anh Pham, Anastasiia Tukova, Amin Hassanzadeh-Barforoushi, Wei Zhang, Yuling Wang
Summary: Cancer-derived small extracellular vesicles (sEVs) are specific subgroups of lipid bilayer vesicles secreted from cancer cells to the extracellular environment. They carry distinct biomolecules (e.g., proteins, lipids and nucleic acids) from their parent cancer cells. Therefore, the analysis of cancer-derived sEVs can provide valuable information for cancer diagnosis. However, the use of cancer-derived sEVs in clinics is still limited due to their small size, low amounts in circulating fluids, and heterogeneous molecular features, making their isolation and analysis challenging.
Review
Chemistry, Multidisciplinary
Jie Wang, Peng Ma, Daniel H. Kim, Bi-Feng Liu, Utkan Demirci
Summary: Exosomes, nano-sized extracellular vesicles secreted by cells, have revolutionized various areas such as liquid biopsies, cancer biology, drug delivery, and immunotherapy. Microfluidic platforms show promise in overcoming the limitations of conventional techniques by offering high precision processing, microscale liquid handling, and integration with various functional units for efficient exosome separation and sensing.
Review
Engineering, Biomedical
Minh-Chau N. Le, Z. Hugh Fan
Summary: Exosomes play a crucial role in cell-to-cell communication and contain valuable information about biological processes. Microfluidic devices have emerged as superior alternatives for exosome isolation due to the limitations of conventional methods.
BIOMEDICAL MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Huiying Fang, Mei Liu, Wei Jiang
Summary: Breast cancer cell-derived exosomes have the potential to be used as biomarkers for continuous biopsies and monitoring in breast cancer. A flexible microfluidic chip with a three-dimensional structure of interlaced cylinders doped with nickel powder was designed for exosome separation. The chip achieved high exosome recovery rate (> 74%) and purity (> 67%) in continuous flow mode, making it a cutting-edge platform for exosome separation and detection.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Chungen Qian, Yujin Xiao, Jie Wang, Yiwei Li, Shunji Li, Bo Wei, Wei Du, Xiaojun Feng, Peng Chen, Bi-Feng Liu
Summary: Exosomes, along with their associated proteins and nucleic acids, are recognized as biomarkers for tumor diagnosis and prognosis. Detecting exosomes is challenging due to their small size and low concentration. A low-cost, rapid, and portable agarose-based microfluidic chip for exosome concentration and in situ exosomal microRNA detection was developed, providing a new avenue for early cancer detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Nanoscience & Nanotechnology
Xin Shi, Wei Tan, Yuwen Lu, Wenfeng Cao, Guorui Zhu
Summary: The newly proposed CCEA microdevice features small size, centrosymmetric structure, and efficient particle/cell focusing capability. Experimental results demonstrate stable fluid distribution, indicating potential applications in basic medical research and clinical diagnosis.
MICROSYSTEMS & NANOENGINEERING
(2021)
Article
Biochemical Research Methods
Mahsa Jalali, Imman Isaac Hosseini, Tamer AbdelFatah, Laura Montermini, Sebastian Wachsmann Hogiu, Janusz Rak, Sara Mahshid
Summary: EVs shed by cancer cells can provide valuable diagnostic information, but clinical use of liquid biopsy based on EVs is hindered by inefficient testing methods. A nanostructured microfluidic device utilizing SERS has been developed for unambiguous identification of EVs from different tumor cell populations, showing promising potential for broader applications in cancer research.
Article
Chemistry, Analytical
Zaidon T. Al-aqbi, Salim Albukhaty, Ameerah M. Zarzoor, Ghassan M. Sulaiman, Khalil A. A. Khalil, Tareg Belali, Mohamed T. A. Soliman
Summary: A novel microfluidic device featuring a nano-junction was developed for on-chip filtration and out-chip collection of blood plasma with a high extraction yield; the device effectively removes blood cells and plasma proteins, allowing for the detection of analytes at low concentrations.
Review
Food Science & Technology
Duo Liang, Wentao Su, Mingqian Tan
Summary: The use of microfluidic intestine-on-a-chip offers a novel way to mimic human intestinal pathophysiology, potentially reducing animal testing. However, research on the effect of bioactive food compounds on intestinal inflammation is still largely unexplored. Standardizing key parameters such as cell types and shear stress is essential for accurate modeling.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Agriculture, Multidisciplinary
Duo Liang, Wentao Su, Xue Zhao, Jiaxuan Li, Zheng Hua, Song Miao, Mingqian Tan
Summary: This study utilized a microfluidic device to synthesize a nanoscale delivery system for encapsulating and colon-target release of fucoxanthin. In vitro experiments demonstrated a pH stimulus-responsive release of fucoxanthin under alkaline conditions and a protective effect on cells damaged by H2O2. The work illustrated the potential of using a microfluidic approach for better biocompatibility and targeting efficacy of the nanodelivery system.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Medical Laboratory Technology
Yuting Zhou, Yehong Liu, Shiyi Xuan, Tianhui Jin, Ke Chen, Zufei Wu, Wentao Su, Liang Chen, Gangjun Zong
Summary: The study found that circSamd4a exhibited a stable downward trend in different specimens, and showed significant advantages as a biomarker for the diagnosis of VC.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Article
Engineering, Chemical
Tongdong Shen, Huijie Bao, Wentao Su, Yan Jiang, Shaoping Tong
Summary: Transition metal oxides and transition metal containing oxides have been extensively researched in heterogeneous catalytic ozonation for water treatment. The catalytic mechanism for typical transition metal oxide - manganese oxide (MnO2) in catalytic ozonation remains unclear. The study found that electron transfer reaction occurs in manganese containing oxides catalytic ozonation when ozone is adsorbed on protonated surface hydroxyl groups of acid sites, but not in magnesium manganese oxides (MgMnxOy). The protonation ability of hydroxyl groups on acid sites and electron transfer ability of acid sites are positively related to Mg content in MgMnxOy.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Wen-Tao Su, Wei Zhao, Maxime Binama, Yue Zhao, Jian-Ying Huang, Xue-Ren Chen
Summary: An investigation was conducted on the cavitation characteristics of the Francis turbine and its influence on the hydraulic stability using flow visualization and acoustic emission methods. As the cavitation coefficient decreases, gap cavitation develops first, followed by airfoil cavitation. Airfoil cavitation causes variations in machine flow and head and reduces the amplitude of pressure pulsation.
Article
Agriculture, Multidisciplinary
Weina Cui, Shanshan Tie, Meng Guo, Fengzhi Qiao, Mingqian Tan, Wentao Su
Summary: In this study, a targeted hyaluronic acid-modified milk exosome-based astaxanthin delivery system was constructed to improve the biocompatibility, stability, and targeted transport properties of astaxanthin. The results showed that this delivery system had a significant targeting effect, enhanced cellular uptake, and effectively alleviated inflammation and inhibited the expression of inflammatory factors.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Review
Green & Sustainable Science & Technology
Kan Kan, Maxime Binama, Huixiang Chen, Yuan Zheng, Daqing Zhou, Wentao Su, Alexis Muhirwa
Summary: This article provides a comprehensive review of the cavitation dynamics and influencing parameters of pump as turbines (PATs), with a focus on their performance in small-scale hydroelectric power plants. The study reveals that the reverse mode of PATs is more susceptible to cavitation, while the conventional mode leads to more damages. Modifying the geometric design parameters of PATs can significantly improve their cavitation performance, which requires further research for the reverse operating mode.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2022)
Article
Food Science & Technology
Yannan Chen, Wentao Su, Shanshan Tie, Lijuan Zhang, Mingqian Tan
Summary: This review highlights the construction of astaxanthin-based delivery systems and the potential benefits in precision nutrition. Well-designed delivery systems can enhance the stability of astaxanthin during food processing and digestion, and can achieve precision nutrition effects for specific populations.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2022)
Review
Multidisciplinary Sciences
Lu Wen, Guoqiang Li, Tao Huang, Wei Geng, Hao Pei, Jialiang Yang, Miao Zhu, Pengfei Zhang, Rui Hou, Geng Tian, Wentao Su, Jian Chen, Dake Zhang, Pingan Zhu, Wei Zhang, Xiuxin Zhang, Ning Zhang, Yunlong Zhao, Xin Cao, Guangdun Peng, Xianwen Ren, Nan Jiang, Caihuan Tian, Zi-Jiang Chen
Summary: With the advancement of single-cell technologies, a vast amount of single-cell omics data has been generated, and numerous bioinformatics algorithms have been developed. These single-cell technologies have found successful applications in various fields, including oncology, assisted reproduction, embryonic development, and plant breeding, and are expected to become a booming business for mechanism research and commercial industry in the future.
Article
Engineering, Biomedical
Duo Liang, Chenyue Liu, Yu Li, Caiyun Wu, Yuling Chen, Mingqian Tan, Wentao Su
Summary: A large-scale engineering preparation strategy has been developed to produce synthetic probiotic membrane vesicles (FX-MVs) for encapsulating fucoxanthin, which can be used for treating colitis. The engineering membrane vesicles showed a significantly higher yield and richer protein content compared to naturally secreted vesicles. FX-MVs exhibited improved gastrointestinal stability of fucoxanthin and effectively inhibited oxidative damage and inflammatory response in vivo.
Article
Food Science & Technology
Meng Guo, Weina Cui, Yuanchao Li, Siyuan Fei, Chaofan Sun, Mingqian Tan, Wentao Su
Summary: In this study, a microfluidic strategy for the preparation of astaxanthin nano-encapsulation system was proposed. The resulting nano-encapsulation system showed better stability and protection effect compared to free astaxanthin under different conditions.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Agriculture, Multidisciplinary
Kuiyou Wang, Shasha Huang, Shanghua Xing, Shida Wu, Hongliang Li, Xu Zhong, Xin Na, Mingqian Tan, Wentao Su
Summary: This study proposes an innovative approach using microfluidic chip to create core-shell microcapsules that contain Lactobacillus plantarum Lp90, which aims to enhance the stability of probiotics in fruit juices. These microcapsules provide a robust barrier and significantly improve the probiotic stability compared to free probiotics, offering a promising solution for probiotic delivery in functional fruit juice beverages.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Food Science & Technology
Hui Wang, Cong Xu, Mingqian Tan, Wentao Su
Summary: Bioengineered gut chips accurately replicate important features of the intestinal microenvironment, making them valuable tools in nutrition research and food technology. This review provides an overview of different approaches to constructing gut-on-chip platforms and explores their applications and potential in studying nutrition metabolism and carotenoid transport.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Shanshan Tie, Siyuan Xiang, Yannan Chen, Fengzhi Qiao, Weina Cui, Wentao Su, Mingqian Tan
Summary: In this study, food-grade nanocarriers were prepared using a one-pot method, which exhibited controllable sizes and good antioxidant capacity. The nanocarriers also showed inhibitory effects on cell damage and could be used for delivering active ingredients.
Article
Green & Sustainable Science & Technology
Maxime Binama, Kan Kan, Hui-Xiang Chen, Yuan Zheng, Daqing Zhou, Wen-Tao Su, Alexis Muhirwa, James Ntayomba
Summary: This study investigates the development mechanism of flow instability in the vaneless space of reversible pump turbines, as well as the impact of machine influx and runner blade number on its transferability. Results show that the pressure pulsation level in the vaneless space decreases with decreasing runner blade number, while a continuous decrease in machine influx leads to a corresponding drop in the pulsation level within the turbine zone before gradually increasing in the runaway zone and dropping again in the deep turbine brake operating zone. The effect of machine flow conditions and runner blade number on the propagation mode of flow instability in the vaneless space to upstream flow zones is more significant than in downstream flow zones.