期刊
BIOLOGY OF REPRODUCTION
卷 102, 期 2, 页码 327-338出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/biolre/ioz183
关键词
estradiol/estradiol receptor; genomics; gene expression; transcriptional regulation; uterus
资金
- NIH/NIDDK [DK058110]
- NIH/NICHD [HD087150]
- Cancer Prevention and Research Institute of Texas (CPRIT) [RP160318/RP190235, RP160319/RP190236, RR170020]
- Cecil H. and Ida Green Center for Reproductive Biology Sciences Endowment
- Lalor Foundation
The ovarian hormones estrogen and progesterone orchestrate the transcriptional programs required to direct functions of the uterus for initiation and maintenance of pregnancy. Estrogen, acting via estrogen receptor alpha, regulates gene expression by activating and repressing distinct genes involved in signaling pathways that regulate cellular and physiological responses including cell division, water influx, and immune cell recruitment. Historically, these transcriptional responses have been postulated to reflect a biphasic physiological response. In this study, we explored the transcriptional responses of the ovariectomized mouse uterus to 17 beta-estradiol (E2) by RNA-seq to obtain global expression profiles of protein-coding transcripts (mRNAs) and long noncoding RNAs (lncRNAs) following 0.5, 1, 2, and 6 hours of treatment. The E2-regulated mRNA and lncRNA expression profiles in the mouse uterus indicate an association between lncRNAs and mRNAs that regulate E2-driven pathways and reproductive phenotypes in the mouse. The transient E2-regulated transcriptome is reflected in the time-dependent shifting of biological processes regulated in the uterus in response to E2. Moreover, high expression of some conserved lncRNAs that are E2 regulated in the mouse uterus are predictive of low overall survival in endometrial carcinoma patients (e.g., H19, KCNQ1OT1, MIR17HG, and FTX). Collectively, this study (1) describes a genomic approach for identifying E2-regulated lncRNAs that may serve critical function in the uterus and (2) provides new insights into our understanding of the regulation of hormone-regulated transcriptional responses with implications in pregnancy and endometrial pathologies. Summary sentence Estrogen regulates protein-coding genes and long noncoding RNAs with expression kinetics that reflect the shifting biological programs and functions of the uterus.
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