Article
Medicine, Research & Experimental
Kari T. Chambers, Michael A. Cooper, Alison R. Swearingen, Rita T. Brookheart, George G. Schweitzer, Carla J. Weinheimer, Attila Kovacs, Timothy R. Koves, Deborah M. Muoio, Kyle S. McCommis, Brian N. Finck
Summary: Loss of Lipin 1 impairs cardiac functional reserve through effects on glycerolipid homeostasis, mitochondrial function, and protein kinase A signaling.
Article
Biochemistry & Molecular Biology
Emilio M. Serrano-Lopez, David Lopez-Martinez, Juan C. Gomez-Fernandez, Antonio Luis Egea-Jimenez, Senena Corbalan-Garcia
Summary: PKC epsilon is highly expressed in mast cells and its activation by acidic phospholipids, specifically phosphatidic acid (PA), leads to increased vesicle degranulation and SNAP23 phosphorylation, indicating a role in the allergic reaction. This study identified a molecular mechanism involving a collection of Arg residues in the C1B domain of PKC epsilon that selectively binds to PA, resulting in plasma membrane translocation and increased vesicle secretion. These findings highlight the importance of PA signaling in regulating PKC epsilon activity, which could be a potential target for controlling allergic responses.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Javier Casas, Clara Meana, Jose Ramon Lopez-Lopez, Jesus Balsinde, Maria A. Balboa
Summary: This study reveals the activation of the TRPC3 channel during macrophage exposure to LPS, which is essential for intracellular Ca2+ release. TRPC3 participates in cytosolic Ca2+ elevations, NF-kappa B activation, and cytokine upregulation. Pharmacological inhibition of TRPC3 reduces LPS-induced systemic inflammation in mice.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
Weijing Gu, Shujuan Gao, Huan Wang, Kaelin D. Fleming, Reece M. Hoffmann, Jong Won Yang, Nimi M. Patel, Yong Mi Choi, John E. Burke, Karen Reue, Michael Airola
Summary: This study elucidates the structural basis of lipin membrane association and identifies a crucial lipin domain with a novel protein fold that is essential for membrane binding and full functionality of lipins.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Laura Brohee, Julie Cremer, Alain Colige, Christophe Deroanne
Summary: The rewiring of lipid metabolism in cancer cells is associated with their increased aggressiveness, making the targeting of lipid metabolism an appealing therapeutic strategy. Lipin-1, a central regulator of lipid homeostasis, has been highlighted for its potential role in cancer progression, both in cancer cells and the tumor microenvironment. Inhibiting Lipin-1 may serve as an adjuvant therapy to enhance the effects of anti-cancer treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yeun Ju Kim, Nivedita Sengupta, Mira Sohn, Amrita Mandal, Joshua G. Pemberton, Uimook Choi, Tamas Balla
Summary: This study reveals that the metabolic fate of newly synthesized phosphatidic acid (PA), the lipid precursor of phosphatidylinositol (PI), is influenced by the fatty acyl-CoA used, leading to preferential routing of the arachidonoyl-enriched species towards PI synthesis. Additionally, the unsaturated forms of PI(4,5P)(2) are replenished faster than the saturated forms during PLC activation, indicating a favored recycling of the unsaturated forms of the PLC-generated hydrolytic products. CDS2 contributes to increased PI resynthesis during PLC activation, while Nir2 contributes to rapid PPIn resynthesis, but the faster re-synthesis of the 38:4 species does not depend on Nir2. Therefore, the fatty acid side-chain composition of the lipid precursors used for PI synthesis is an important determinant of their metabolic fates and the maintenance of the unique fatty acid profile of PPIn lipids.
Review
Biochemistry & Molecular Biology
Tamotsu Tsukahara, Shigeyuki Imamura, Toshiro Morohoshi
Summary: Osteoarthritis is a chronic degenerative joint disease with no cure currently available. However, recent research suggests that cPA may have potential as a treatment, as it promotes various cellular responses including proliferation, migration, and survival.
Article
Biochemistry & Molecular Biology
Pawan Sharma, Santosh K. Yadav, Sushrut D. Shah, Elham Javed, John M. Lim, Shi Pan, Ajay P. Nayak, Reynold A. Panettieri, Raymond B. Penn, Taku Kambayashi, Deepak A. Deshpande
Summary: This study demonstrated that inhibition of DGK disrupted the DAG:PA ratio, resulting in the inhibition of Gq-PLC activation in a negative feedback manner, thus protecting against ASM contraction.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Usman Ali, Shaoping Lu, Tarig Fadlalla, Sidra Iqbal, Hong Yue, Bao Yang, Yueyun Hong, Xuemin Wang, Liang Guo
Summary: This review focuses on the structure, acting mechanism, and functional significance of several phospholipase families in plant growth, development, and stress responses. The article also highlights some critical knowledge gaps in the action mechanism, metabolic and signaling roles of these phospholipases and their products in the context of plant growth, development, and stress responses.
PROGRESS IN LIPID RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Fumi Hoshino, Fumio Sakane
Summary: AP180 protein is crucial for driving endocytosis by interacting with clathrin and it can be selectively regulated by 18:0/22:6-PA, which attenuates its interaction with clathrin. This novel mechanism sheds light on the disassembly of AP180/clathrin-containing cages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Chemistry, Organic
Yuki Kitaura, Rina Ueshima, Kanae Sano, Takashi Kikuma, Yoichi Takeda
Summary: This study demonstrated the efficient synthesis of CDP-DAG, a crucial precursor in forming lipid membranes, using sulfonylimidazolium salt as an activator. The method provides valuable insights into the biosynthesis and regulatory mechanisms of glycerophospholipids.
TETRAHEDRON LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Takuya Kano, Ryosuke Tsumagari, Akio Nakashima, Ushio Kikkawa, Shuji Ueda, Minoru Yamanoue, Nobuyuki Takei, Yasuhito Shirai
Summary: DGK beta is an enzyme that plays a crucial role in inducing neurite outgrowth by activating the mTORC1 pathway, contributing to higher brain functions such as emotion and memory. Interaction with RalA and activation of PLD and mTORC1 are key steps in DGK beta-induced neurite outgrowth.
Article
Biochemistry & Molecular Biology
Yaroslav Kolesnikov, Serhii Kretynin, Yaroslava Bukhonska, Igor Pokotylo, Eric Ruelland, Jan Martinec, Volodymyr Kravets
Summary: Cells sense extracellular signals and regulate their metabolism and physiology accordingly. The plasma membrane plays a crucial role in this process by acting as an informational barrier and responding to regulatory inputs. Phosphatidic acid (PA) is a well-known intracellular messenger that regulates various cellular processes and is involved in plant growth, development, and stress tolerance. This review focuses on the primary signaling events triggered by rapid PA accumulation in plant cells, and discusses its role in mediating the response to hormones and hormone-like regulators.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Fumi Hoshino, Maika Nakayama, Masataka Furuta, Chiaki Murakami, Ayumu Kato, Fumio Sakane
Summary: This study identified the binding between phospholipase C (PLC) beta 1 and phosphatidic acid (PtdOH), with the strongest interaction observed with 16:0/16:0-PtdOH. Mutant experiments confirmed the importance of this binding, and it was found that 16:0/16:0-PtdOH can regulate the function of PLC beta 1.
Review
Plant Sciences
Oludoyin Adeseun Adigun, Muhammad Nadeem, Thu Huong Pham, Linda Elizabeth Jewell, Mumtaz Cheema, Raymond Thomas
Summary: Plant pathogens are a significant threat to food security and the agricultural economy, with understanding and utilizing plant immune systems being crucial for controlling pathogens and promoting sustainable agriculture. Lipids play a key role in plant defense against pathogens, yet there is limited information available on lipid signaling targets in the scientific literature.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Biochemistry & Molecular Biology
Javier Casas, Jesus Balsinde, Maria A. Balboa
Summary: Phosphorylation of IVA cytosolic phospholipase A(2)alpha is crucial for its translocation to membranes rich in phosphatidylinositol (4,5) bisphosphate. This phosphorylation process regulates the mobilization of arachidonic acid and the synthesis of prostaglandins.
Article
Biochemistry & Molecular Biology
Ines Barahona, Patricia Rada, Silvia Calero-Perez, Ruben Grillo-Risco, Laura Pereira, M. Carmen Soler-Vazquez, Laura Maria Lalglesia, Maria J. Moreno-Aliaga, Laura Herrero, Dolors Serra, Carmelo Garcia-Monzon, Agueda Gonzalez-Rodriguez, Jesus Balsinde, Francisco Garcia-Garcia, M. Pilar Valdecantos, Angela M. Valverde
Summary: The study demonstrates that PTP1B deficiency protects OCs against lipotoxicity by promoting antioxidant defenses and cellular processes, resulting in the formation and dynamics of LDs.
CELL DEATH AND DIFFERENTIATION
(2022)
Review
Cell Biology
Miguel A. Bermudez, Laura Pereira, Cristina Fraile, Laura Valerio, Maria A. Balboa, Jesus Balsinde
Summary: In this review, the effects of monounsaturated hexadecenoic fatty acids, particularly palmitoleic acid, on metabolic diseases are discussed. Palmitoleic acid has been described as a lipokine that regulates various metabolic processes. The biological effects of sapienic acid and hypogeic acid are still poorly understood, but accumulating evidence suggests their importance in metabolic regulation.
Review
Biochemistry & Molecular Biology
Alma M. Astudillo, Maria A. Balboa, Jesus Balsinde
Summary: Lipid homeostasis perturbations and conditions favoring oxidative stress are important in the inflammatory response. Recent research has focused on lipid signaling in oxidative stress-mediated responses and inflammation, including phagocytosis and ferroptosis. The synthesis of oxygenated metabolites of arachidonic acid and other polyunsaturated fatty acids is a common event in these responses. Advances in this area highlight the importance of compartmentalization of enzymes and lipid substrates in shaping the appropriate response. Understanding the role of specific enzymes, such as iPLA213, in oxidative stress and inflammation may lead to new therapeutic interventions.
PROGRESS IN LIPID RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Miguel A. Bermudez, Julio M. Rubio, Maria A. Balboa, Jesus Balsinde
Summary: This study investigates the metabolic fate of arachidonic acid (AA) pools in macrophages during cellular activation. The membrane phospholipid AA pool is shown to be the major, and possibly the only, source of releasable AA during acute stimulation. However, the AA pool in triacylglycerol is used at a later stage to replenish the phospholipid pools.
Article
Biochemistry & Molecular Biology
Nagore de Pablo, Clara Meana, Javier Martinez-Garcia, Pablo Martinez-Vicente, Manuel Albert, Susana Guerra, Ana Angulo, Jesus Balsinde, Maria A. Balboa
Summary: This study reveals that interferons regulate the expression of lipin-2, which can inhibit viral replication and regulate inflammation. Furthermore, LPIN2 expression levels are negatively correlated with the severity of COVID-19.
Article
Gastroenterology & Hepatology
Irma Garcia-Martinez, Rosa Alen, Laura Pereira, Adrian Povo-Retana, Alma M. Astudillo, Ana B. Hitos, Isabel Gomez-Hurtado, Eduardo Lopez-Collazo, Lisardo Bosca, Ruben Frances, Ignacio Lizasoain, Maria Angeles Moro, Jesus Balsinde, Manuel Izquierdo, Angela M. Valverde
Summary: This study reveals that small extracellular vesicles (sEV) released by hepatocytes under NAFLD conditions induce liver inflammation and insulin resistance in hepatocytes through paracrine hepatocyte-macrophage-hepatocyte crosstalk. These sEV act as transporters of saturated fatty acids (SFAs) and potent inducers of liver inflammation. Liver inflammation mediated by sEV can be attenuated by inhibiting Toll-like receptor-4 (TLR4). Evidence of macrophage-hepatocyte interactome was also found in patients with NAFLD, highlighting the relevance of sEV in SFAs-mediated lipotoxicity in NAFLD.
Article
Biochemistry & Molecular Biology
Patricia Monge, Alma M. Astudillo, Laura Pereira, Maria A. Balboa, Jesus Balsinde
Summary: This study investigates the incorporation and redistribution of DHA in mouse macrophages. DHA is initially taken up by choline glycerophospholipids and transferred to ethanolamine glycerophospholipids through prolonged incubation. Mass spectrometry analysis reveals a high abundance of DHA in ether phospholipids. Stimulation of macrophages leads to decreases in DHA-containing PC and PI species, while an unusual DHA-containing species, PI(20:4/22:6), markedly increases.
Article
Cell Biology
Emma Barroso, Marta Diaz, Ana Cristina Reguera, Mona Peyman, Jesus Balsinde, Javier Jurado-Aguilar, Meijian Zhang, Adel Rostami, Xavier Palomer, Lourdes Ibanez, Manuel Vazquez-Carrera
Summary: Placentas from newborns that are SGA display multiple pathological characteristics, and placental amino acid transport regulated by mTOR plays a key role in fetal growth. The effects of ER stress on the mTOR pathway and amino acid transporters are not well understood.
CELL COMMUNICATION AND SIGNALING
(2023)
Meeting Abstract
Gastroenterology & Hepatology
Rosa Alen Alonso, Irma Garcia-Martinez, Laura Pereira, Adrian Povo-Retana, Isabel Gomez-Hurtado, Eduardo Lopez-Collazo, Lisardo Bosca, Ruben Frances, Jesus Balsinde, Manuel Izquierdo, Angela M. Valverde
Meeting Abstract
Gastroenterology & Hepatology
Pilar Valdecantos, Ines Barahona, Silvia Calero, Ruben Grillo-Risco, Laura Pereira, Carmen Soler, Lara LaIglesia, M. Jesus Moreno-Aliaga, Laura Herrero, Dolors Serra, Carmelo Garcia-Monzon, Agueda Gonzalez, Jesus Balsinde, Francisco Garcia-Garcia, Angela Martinez Valverde
JOURNAL OF HEPATOLOGY
(2022)
Article
Medicine, General & Internal
Ignacio Alcalde, Cristina Sanchez-Fernandez, Carla Martin, Nagore De Pablo, Nahla Jemni-Damer, Gustavo V. Guinea, Jesus Merayo-Lloves, Susana Del Olmo-Aguado
Summary: The objective of this study is to analyze the current data from clinical trials using stem cells to treat human retinal diseases. Although there are currently no approved therapies based on stem cell transplantation for patients, the outcomes of many clinical trials show promising results in terms of implantation techniques and long-term safety. Quantifiable results related to visual function improvement are expected in the coming years.
MEDICINA-LITHUANIA
(2022)
Article
Biochemistry & Molecular Biology
Chenchen Bian, Xiangtong Yuan, Caihong Zeng, Jian Sun, Gen Kaneko, Hong Ji
Summary: This study investigated the mechanism of docosahexaenoic acid (DHA)-induced apoptosis mediated by mitophagy, using grass carp as an animal model. The results showed that inhibition of mitophagy alleviated apoptosis and eliminated the inhibition of lipid accumulation induced by DHA. Mechanistically, DHA induced mitophagy by activating the PPAR gamma-LC3-BNIP3 pathway. Inhibition of PPAR gamma decreased autophagy-related gene expression and prevented BNIP3/NIX-mediated mitophagy-induced apoptosis, thereby alleviating the inhibition of lipid accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2024)
Article
Biochemistry & Molecular Biology
Venkatesan Ramya, Karuppiah Prakash Shyam, Arulanandu Angelmary, Balamuthu Kadalmani
Summary: This study reveals that Lauric acid (LA) exerts an epigenetic regulation and metabolic reprogramming on SH-SY5Y neuroblastoma cells through modulation of lncRNA HOTAIR, remodeling of chromatin H3K4 tri-methylation and regulation of glucose uptake by controlling NF-kappa B activation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2024)
Article
Biochemistry & Molecular Biology
Sreelekshmi Sreekumar, Karyath Palliyath Gangaraj, Manikantan Syamala Kiran
Summary: This study investigates the intricate interplay between angiogenic regulation and the browning of white adipocytes. The findings reveal that concurrent activation of angiogenesis is necessary for inducing browning of white adipocytes. The study also highlights the role of Vascular endothelial growth factor (VEGF) in promoting angiogenesis and triggering the browning process through the activation of Estrogen receptor alpha (ER alpha) signaling pathway.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2024)
Article
Biochemistry & Molecular Biology
Zanxia Cao, Liling Zhao, Mingcui Chen, Zhihong Shi, Lei Liu
Summary: This study investigated the translocation process of cholesterol/calcitriol in bacterial membranes and their effects on membrane structure. Calcitriol facilitated water transport across the membrane, while cholesterol had the opposite effect. These findings contribute to a better understanding of the relationship between cholesterol/calcitriol concentrations, lipid bilayer structure, and water permeation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2024)
Article
Biochemistry & Molecular Biology
Xiaozhen Guo, Jiawen Wang, Hualing Xu, Yangyang Wang, Yutang Cao, Yingquan Wen, Jiaqi Li, Yameng Liu, Kanglong Wang, Jue Wang, Xianchun Zhong, Chuying Sun, Yongxin Zhang, Jingyi Xu, Cuina Li, Pengxiang Mu, Lingyan Xu, Cen Xie
Summary: This study aims to investigate the role of the gut microbiota-bile acid axis in regulating the diurnal rhythms of metabolic homeostasis and assess the impact of obesity on them. The results show that high fat diet feeding and Leptin gene deficiency disrupt the rhythmic patterns of insulin sensitivity and serum total cholesterol levels. The study provides compelling evidence for the association between diurnal rhythm of insulin sensitivity and gut microbiota-bile acid axis, and elucidates the deleterious effects of obesity on gut microbiome-bile acid metabolism.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2024)
Article
Biochemistry & Molecular Biology
Giuseppe Pepe, Maria Cotugno, Federico Marracino, Luca Capocci, Ludovica Pizzati, Maurizio Forte, Rosita Stanzione, Pamela Scarselli, Alba Di Pardo, Sebastiano Sciarretta, Massimo Volpe, Speranza Rubattu, Vittorio Maglione
Summary: The study found that enzymes involved in sphingolipid metabolism show abnormal expression in the cardiac tissue of hypertensive rat models, which may be related to the susceptibility to cardiac damage.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2024)